Fortification of Tris Extender with Mifepristone, Sericin and Taurine Improves Velocity and Kinematics of Fresh and Frozen-thawed Bovine Spermatozoa 

2021 ◽  
Author(s):  
Devangana Chaturvedi ◽  
A.J. Dhami ◽  
D.V. Chaudhari
Keyword(s):  
Sperm Quality ◽  
Semen Analysis ◽  
Freezing Stress ◽  
Computer Assisted ◽  
Motile Sperm ◽  
Sperm Analysis ◽  
Head Displacement ◽  
Antioxidant Additives ◽  
The Mean ◽  
Motion Characteristics

Background: The evaluation of bull fertility essentially involves semen analysis and conception rates among inseminated females. The conventional evaluation is relatively imprecise, time consuming and subjected to individual variations, while computer assisted sperm analysis (CASA) is a quite faster, precise and objective tool to identify differences in sperm motility and velocity/kinematics attributes and avoids subjective errors. The present study was planned to evaluate and compare the CASA traits of fresh and frozen-thawed semen of Gir and Murrah bulls in Tris extender without and with different antioxidant additives by adopting Biovis CASA.Methods: The semen ejaculates with greater than 75% initial motility from 3 Gir and 3 Murrah bulls were split-diluted at 100 million sperm per ml using Tris-citrate-fructose-yolk-glycerol (TFYG) extender without (control) and with three antioxidant additives, viz., Mifepriston (10 µg/ml), Sericin (5 mg/ml) and Taurine (4 mg/ml), were filled in French mini straws and frozen in liquid nitrogen vapour using a programmable biofreezer. The freshly diluted and frozen-thawed semen samples were assessed for sperm motion characteristics, velocity/kinematics using Biovis CASA. Result: The mean percentages of total motile spermatozoa, irrespective of additives, in freshly diluted and frozen-thawed semen were 85.50±0.92 and 48.76±1.69 for Gir bulls and 85.03±0.72 and 52.61±1.46 for Murrah bulls, respectively. The mean total motile as well as rapid and slow progressive motile sperm percentage were significantly enhanced by fortification of TFYG extender with Mifepristone than in control extender, while values for Sericin and Taurine were intermediary and statistically similar to Mifepristone. The per cent decline in total motile sperm due to freezing-thawing was more or less same in both the breeds for all the extender additives, being lowest in Mifepristone supplemented extender in cattle (32.27 vs 48.81%) and buffalo (31.27 vs 43.14%) semen. The rapid and slow progressive motile sperm also followed the same pattern. The values of VAP and VCL were significantly (p less than 0.05) higher and VSL lower in Murrah than Gir breed at post-thaw stage. The overall mean linearity (%), straightness (%), beat-cross frequency (hz), lateral head displacement (µm), wobbling index (%), dancing velocity (µm2/s) and dancing mean (µm2/s) of sperm were higher in Murrahs than in Gir semen with significant (p less than 0.01) difference at post-thaw stage. The fortification of extender with Mifepristone improved all these traits at post-thaw stage compared to other additives and control TFYG extender. The per cent decline in these traits due to freezing stress was much lower in buffalo sperm than the cattle sperm particularly with Mifepristone fortification. It was concluded that freezing-thawing stress adversely affected the motion and kinematics of both cattle and buffalo sperm and that Tris extender fortified with antioxidant additives, particularly Mifepristone @ 10 µg/ml, protected sperm from adverse effect of dilution and cryopreservation with improved post-thaw sperm quality.

17 Increased inbreeding levels negatively affect sperm kinetics and motility in Purebred Spanish horses

2021 ◽  
Vol 33 (2) ◽  
pp. 116
Author(s):  
Y. Pirosanto ◽  
A. Molina ◽  
M. Valera ◽  
J. Dorado ◽  
E. Terán ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Quality ◽  
Reproductive Traits ◽  
Study Groups ◽  
Inbreeding Coefficient ◽  
Computer Assisted ◽  
Effective Population ◽  
Mean Velocity ◽  
Sperm Analysis ◽  
Head Displacement

Reproductive performance is one of the key factors in livestock production. It is well known that reproductive traits are influenced by several genetic factors, such as the increase of individual inbreeding levels, which are associated with changes in sperm motility and shape in several species. In horses, the increase in inbreeding is a common problem because of the reduction in effective population size and the increase in selection intensity observed in several breeds. However, studies assessing the effect of high levels of inbreeding on the sperm quality of stallions are scarce. In the present study, we aimed to determine the effect of increased inbreeding levels and age on the sperm motility patterns of Purebred Spanish horses (PRE). We performed kinetic characterisation of 557 sperm samples of 82 PRE stallions aged between 3 and16 years, using computer-assisted sperm analysis (Androvision™, Minitube). We evaluated 5 parameters in 6 different fields per sample: curved line velocity (VCL, µm/s), velocity average path (VAP, µm/s), velocity straight line (VSL, µm/s), amplitude of lateral head displacement (ALH, µm), and beat-cross frequency (BCF, Hz). We determined the pedigree-based inbreeding coefficient (Fped) based on ∼300,000 PRE pedigree records to evaluate the inbreeding effect. Individuals were separated into 2 groups: highly inbred (n=339) and lowly inbred (n=218) according to an F value of 12.5%. Differences between groups were analysed using a generalized linear model. The analysis did not show significant differences (P>0.05) in the variables analysed with respect to the age of stallions. However, VAP, VCL, and AHL were lower in highly inbred than in lowly inbred animals (P<0.05), suggesting less velocity and amplitude of head displacement. In the case of BCF, no significant differences (P>0.05) were observed between the two study groups. In conclusion, age did not affect sperm quality parameters in the age group of stallions analysed. In addition, we demonstrated that high inbreeding coefficient reduced the mean velocity and trajectory pattern of spermatozoa in PRE.

72 EFFECT OF SINGLE-LAYER CENTRIFUGATION WITH EQUIPURE™ ON MOTILITY KINEMATICS OF FROZEN - THAWED DONKEY SPERM

2013 ◽  
Vol 25 (1) ◽  
pp. 183 ◽  
Author(s):  
I. Ortiz ◽  
J. Dorado ◽  
D. Acha ◽  
L. Ramirez ◽  
M. Urbano ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Single Layer ◽  
Bottom Layer ◽  
Computer Assisted ◽  
Kinematic Parameters ◽  
Sperm Analysis ◽  
Progressive Motility ◽  
Head Displacement ◽  
Straight Line ◽  
Computer Assisted Sperm Analysis

Single-layer centrifugation (SLC) with EquipureTM Bottom Layer has been used to enhance the quality of stallion semen samples; however, no studies have been performed on donkeys. The aim of this study was to determine if SLC with EquipureTM Bottom Layer improves kinematic parameters on frozen–thawed donkey sperm. Semen was collected from 4 Andalusian donkeys by artificial vagina. Three ejaculates from each donkey were centrifuged with EquiproTM, supernatant was removed, and pellet was re-extended in the freezing medium GentTM to a final concentration of 200 × 106 spermatozoa per milliliter. Sperm were slowly cooled to 5°C for 2 h, loaded in 0.5-mL plastic straws, and frozen in liquid-nitrogen vapors. After at least one week of storage, straws were thawed in a water bath at 37°C for 30 s. After thawing, semen samples were divided in 2 aliquots: aliquot 1 was used as such (control) and aliquot 2 was processed by SLC using EquipureTM Bottom Layer. Computer-assisted sperm analysis was performed, and sperm kinematics total motility (%), progressive motility (%), curvilinear velocity (VCL; µm s–1), velocity straight line (VSL; µm s–1), velocity average path (VAP; µm s–1), linearity (LIN; %), straightness (STR; %), wobble (WOB; %), lateral head displacement (ALH; µm), and beat cross frequency (BCF; Hz) were statistically compared using GLM model between frozen–thawed semen samples processed or not with EquipureTM. Results were expressed as mean ± standard error. Significant differences (P < 0.05) were found between SLC-selected and unselected semen for total motility (77.44 ± 5.83 v. 58.89 ± 6.07), progressive motility (76.88 ± 4.52 v. 56.59 ± 5.44), VCL (137.50 ± 0.75 v. 133.0 ± 0.99), LIN (69.43 ± 0.31 v. 68.23 ± 0.41), STR (78.45 ± 0.29 v. 76.90 ± 0.37), WOB (85.06 ± 0.18 v. 83.91 ± 0.26), ALH (2.76 ± 0.01 v. 2.44 ± 0.01), and BCF (9.13 ± 0.05 v. 8.53 ± 0.06), respectively. No significant differences were observed for VSL (102.89 ± 0.70 v. 104.32 ± 0.95) and VAP (123.21 ± 0.71 v. 121.50 ± 0.98). Most of the computer-assisted sperm analysis parameters used in the present study have been previously identified as reliable markers of sperm motility in relation to sperm quality and fertility. It has also been reported that VCL appears to be critical for the formation of the sperm reservoir and penetration of the zona pellucida. In addition, other variables improved in the SLC-selected samples have been described as measure of progressivity (LIN, STR) and spermatozoa vigor (BCF, ALH). These preliminary results suggest an additional option for improving sperm quality in donkey semen doses. In conclusion, SLC with EquipureTM can be used to enhance kinematic parameters on frozen–thawed donkey sperm.

Computer assisted sperm analysis of fresh and frozen-thawed buffalo semen and their interrelationship

2016 ◽  
Vol 50 (1) ◽  
Author(s):  
J. B. Patel ◽  
A. J. Dhami
Keyword(s):  
Sperm Motility ◽  
Computer Assisted ◽  
Motile Sperm ◽  
Mean Values ◽  
Sperm Analysis ◽  
Head Displacement ◽  
Straight Line ◽  
Buffalo Semen ◽  
Live Sperm ◽  
Fresh Semen

Sixty semen ejaculates from 10 mature bulls, 5 each of Jafarabadi and Mehsana breed, were studied for sperm motility and velocity parameters of fresh and frozen-thawed spermatozoa using computer assisted sperm analyzer (CASA). The mean values of motile and progressively motile spermatozoa observed in fresh semen of Jafarabadi and Mehsana bulls (79.77±1.62 and 61.80±1.85, and 78.90±1.22 and 61.37±1.58%) were highly significantly (P<0.01) reduced (51.20±1.57 and 33.20±1.45, and 52.10±1.70 and 34.30±1.54 %, respectively) in post-thawed semen. The average path velocity, straight line velocity and curvilinear velocity (μm/sec) of spermatozoa of Jafarabadi and Mehsana bulls noted in fresh semen were also reduced highly significantly (P<0.01) in frozen-thawed semen. Among the other velocity parameters, amplitude of lateral head displacement (μm), elongation (%) and medium motile sperm (%) increased, while beat-cross frequency (Hz), straightness (%), linearity (%), sperm area (μm<sup>2</sup>) and rapidly motile sperm (%) decreased significantly in post-thawed sperms when compared with the fresh sperm of both Jafarabadi and Mehsana bulls. The initial motility and live sperm per cent were significantly correlated with CASA traits of fresh and frozen-thawed semen, and all the sperm motility and velocity traits of fresh and frozen-thawed semen assessed by CASA were significantly interrelated among both the breeds. The interrelationships were stronger in Mehsana bulls as compared to Jafarabadi bulls.

scholarly journals The structure of subpopulations of stallion spermatozoa after thawing differs between good and poor freezers

2020 ◽  
Vol 65 (No. 11) ◽  
pp. 403-410
Author(s):  
Filipa Bubenickova ◽  
Jiří Šichtař ◽  
Linda Nováčková ◽  
Jitka Sirohi ◽  
Ondřej Šimoník
Keyword(s):  
Cluster Analysis ◽  
Sperm Quality ◽  
Computer Assisted ◽  
Motile Sperm ◽  
Kinematic Parameters ◽  
Sperm Analysis ◽  
Total Proportion ◽  
And Cluster Analysis ◽  
The Times ◽  
The Individual

The aim of this study was to evaluate differences in the presence of sperm subpopulations in frozen-thawed semen in stallions with different freezability. The motility of individual spermatozoa of 24 stallions from 15 breeds was evaluated using computer-assisted sperm analysis (CASA) immediately after thawing (T0) and after 30 min of incubation (T30). In accordance with our previous studies, samples were initially divided based on their total motility into categories of good (GF) and poor (PF) freezers. K-means cluster analysis of kinematic parameters of spermatozoa was used to divide motile sperm (n = 57 630) into three subpopulations. Analysis of variance was used to evaluate differences in the subpopulations between GF and PF stallions at the times of incubation T0 and T30. Statistically significant differences were found in most kinematic parameters between PF and GF stallions as well as between the times of incubation T0 and T30 (P &lt; 0.05). Spermatozoa of good freezers are represented more frequently in the fast and medium fast subpopulations and are faster and more linear than those of poor freezers (P &lt; 0.05). Sperm from PF stallions were more strongly affected by longer incubation. The percentage of sperm in the fast and medium fast subpopulations was lower in samples from PF stallions, but assessment of the motility parameters in particular sperm subpopulations revealed that these sperm had good velocity. Poor freezer samples had lower sperm quality due to a reduced total proportion of motile sperm, and these samples were more sensitive to prolonged time after thawing. Thus, an efficient sperm selection method or a special insemination technique should be used for obtaining doses from stallions with poor freezability. Our study showed that the CASA system and cluster analysis are promising tools for better understanding the significant differences in the individual stallion freezability, and further research should be focused on their application in the field.

scholarly journals Advanced Techniques of Bovine Semen Analysis

2018 ◽  
Vol 75 (1) ◽  
pp. 58
Author(s):  
Mihai CENARIU ◽  
Emoke PALL ◽  
Mihai BORZAN ◽  
Liviu BOGDAN ◽  
Ioan GROZA
Keyword(s):  
Quality Evaluation ◽  
Sperm Quality ◽  
Semen Analysis ◽  
Computer Assisted ◽  
Sperm Analysis ◽  
Bovine Semen ◽  
Bull Semen ◽  
Proper Training ◽  
Computer Assisted Sperm Analysis ◽  
Automated Methods

The aim of the study was to assess bull semen fertility parameters using the classical techniques of sperm quality evaluation (density, motility, viability, and morphology, evaluated by light microscopy, in addition to concentration, evaluated via the hemocytometer and microspermatocrit), as well as advanced techniques, like computer assisted sperm analysis (CASA) and flow cytometry. Results obtained for classical techniques were comparable to those obtained by automated methods, without significant differences between parameters. The classical methods were inexpensive but required more time and attention, while the operator’s experience was a key element for accurate assessment of sperm parameters. The advanced techniques were fast and objective, but required expensive equipment and dedicated personnel, with proper training in the field. Therefore, classical techniques are suitable for clinics where occasional evaluation of bulls’ fertility parameters is performed, while the advanced methods should be implemented in semen companies, as well as in fertility clinics and research laboratories.

Correlations of Motion Characteristics and KinematicAttributes of Fresh and Frozen-thawed Spermatozoaof Gir Bulls

2019 ◽  
Vol 15 (01) ◽  
pp. 9-13
Author(s):  
P K Pathak ◽  
A J Dhami ◽  
D V Chaudhari
Keyword(s):  
Sperm Motility ◽  
Sperm Quality ◽  
Motile Sperm ◽  
Kinematic Parameters ◽  
Head Displacement ◽  
Straight Line ◽  
Motion Characteristics ◽  
Kinematics Parameters ◽  
Fresh Semen ◽  
Lateral Head

This investigation was carried out on semen of three healthy mature breeding bulls of Gir breed to evaluate the interrelationships among sperm quality attributes of fresh and frozen-thawed semen assessed by Biovis CASA. The ejaculates (n = 24) having >75% initial motility were diluted @80 million sperm/mL using TFYG extender, filled in French mini straws, and were frozen using a programmable bio freezer after 4 hours of equilibration. The straws were thawed in a water bath at 37°C for 30 sec. The freshly diluted and frozenthawed samples were assessed for routine subjective tests and various motion characteristics/kinematics by Biovis CASA. The Pearson’s correlations for sperm motility and velocity/kinematic parameters of total motile sperm as well as of progressively motile sperm were studied in freshly diluted and frozen-thawed semen. In fresh semen, total motile sperm assessed by CASA had significant (p less than 0.05, 01) correlations with rapid progressive motile sperm (r = 0.46), wobbling index (r = 0.52) and dancing frequency (r = -0.43) in fresh semen. In frozen-thawed semen, it was significantly correlated only with linearity (r = 0.46). The rapid progressive motile sperm in both fresh (r = 0.41 to 0.92) and frozen-thawed (r = 044 to 0.88) semen, however, had significant correlations with most of their velocity traits. Further, the average path velocity (VAP), curvilinear velocity (VCL), straight line velocity (VSL), linearity (LIN), straightness (STR), wobbling (WOB), beat-cross frequency (BCF), amplitude of lateral head displacement (ALH), and dancing mean (DNM) of sperm showed significant positive or negative interrelationships among each other in both fresh (r = 0.41 to 0.91) as well as post-thawed (r = 0.44 to 0.90) semen. Moreover, the correlations of motility and kinematics parameters of total motile sperm in both fresh and frozen-thawed semen were highly significant with velocity/kinematics traits of only progressively motile sperm, and the velocity traits among only motile sperm were highly significantly interrelated in both fresh (r = 0.46 to 0.98) and frozen-thawed (r = 0.43 to 0.93) semen of Girbulls, though the magnitudes of correlations were lower in frozen-thawed semen as compared to fresh semen. Thus, CASA analysis offresh semen for motility and velocity traits could predict the post-thawed sperm motility and velocity/kinematics of bovine semen.

scholarly journals Computer Assisted Sperm Analysis: A Review

2020 ◽  
Vol 1 (2) ◽  
pp. 60-66
Author(s):  
Agustinus Agustinus ◽  
Cennikon Pakpahan
Keyword(s):  
Semen Analysis ◽  
Sperm Concentration ◽  
World Health ◽  
Computer Assisted ◽  
Morphological Examination ◽  
Sperm Analysis ◽  
Head Displacement ◽  
Sperm Suspension ◽  
Computer Assisted Sperm Analysis ◽  
Time Required

Background: A semen analysis exam is a routine check that is done to evaluate fertility. The World Health Organization (WHO) recommended a manual method to obtain objective and standardized values. However, sometimes some errors can be found with this method such as motility. Computer-Assisted Sperm Analysis offers a way to reduce inaccuracies that often occur with manual methods. Reviews:  CASA systems consist of a microscope which connected into a camera to detect microscopic sperm suspension images and a computer installed with special software to extract desired information and produce the desired output. In the morphological examination, CASA can reduce the coefficient of variation (CV) which is around 4.8% compared to the manual examination but the time required is longer than manual. CASA can visualize and evaluate sperm kinematics. Various parameters such as mean path velocity (VAP), curved velocity (VCL), straight-line velocity (VSL), lateral head displacement amplitude (ALH), or beat cross frequency can be obtained, and this allows a detailed view into the behavior of individual sperm. The limitations affecting CASA's ability to provide accurate results for sperm concentration and percentages of motile or progressively motile spermatozoa. Summary: CASA has several advantages through its ability to calculate more detailed parameters, but a qualified operator must operate it because there is some potential for misinterpretation. The combination of The Manual and CASA is highly recommended for better results.

100 The evolution and premature hyperactivation of kinetic sperm subpopulations are affected by inbreeding level in Retinta cattle

2021 ◽  
Vol 33 (2) ◽  
pp. 157
Author(s):  
E. Teran ◽  
A. Molina ◽  
Z. P. Rodriguez ◽  
J. Dorado ◽  
S. D. Peyrás
Keyword(s):  
Sperm Quality ◽  
Mean Amplitude ◽  
Computer Assisted ◽  
Genetic Trait ◽  
Sperm Analysis ◽  
Head Displacement ◽  
Significant Difference ◽  
Genomic Inbreeding ◽  
Inbreeding Level ◽  
Close Relatives

Computer-assisted sperm analysis (CASA) has become a powerful tool to study sperm heterogeneity because it allows to cluster individual sperm in subpopulations (Sp) according to their kinetic parameters, which are associated with fertility and sperm quality. In cattle, inbreeding (mating between close relatives) affects sperm quality. Inbreeding estimation has become more accurate with the increasing availability of genomic methodologies, such as the detection of runs of homozygosity (ROH). Additionally, genomic inbreeding values (FROH) allow us to determine which metabolic pathways are differentially affected by this genetic trait. The aim of this study was to assess the effect of inbreeding on evolution of sperm Sp over time in cattle. Sperm samples (n=100) from 50 Retinta bulls (two replicates) were analysed in a sperm longevity experiment at time 0 (T0, after thawing), 1 (T1) and 2 (T2) h. At each time point, eight parameters were measured using a CASA system (Sperm Class Analyzer 5.4), including curvilinear velocity (VCL, μm/s), straight-line velocity (VSL, μm/s); average path velocity (VAP, μm/s), percentage of linearity (LIN,%: VSL/VCL), percentage of straightness (STR,%: VSL/VAP), wobble coefficient (WOB,%: VAP/VCL), mean amplitude of lateral head displacement (ALH, μm) and beat-cross frequency (BCF, Hz). The presence of Sp was determined by a two-step multivariate analysis including non-hierarchical followed by hierarchical analysis in 80,154 motile sperm. Four sperm Sp were identified: Sp1 (rapid and highly progressive sperm); Sp2 (progressive sperm with intermediate speed); Sp3 (slow and non-progressive sperm); and Sp4 (fastest, hyperactive-like, non-progressive sperm). To determine the effect of inbreeding, individuals were clustered into lowly (FROH &lt; 0.125, n=27) and highly (FROH &gt; 0.125, n=23) inbred individuals. After thawing (T0), the percentage of sperm Sp4 was higher and showed premature hyperactivation in highly inbred animals, which was previously associated with reduced fertility (Table 1). However, highly inbred individuals showed an increased percentage of rapid (Sp1) and intermediate (Sp2) progressive sperm after T1 and T2 incubation, respectively, compared with lowly inbred bulls (P&lt;0.001). These results suggest that sperm velocity and progression persist for longer in highly inbred bulls, at least after a short incubation. In conclusion, our results demonstrate that inbreeding affects sperm motility pattern and premature hyperactivation in cattle. Table 1. Percentage of subpopulations (Sp) by inbreeding group and time Time Sp1 Sp2 Sp3 Sp4 Highly inbred Lowly inbred Highly inbred Lowly inbred Highly inbred Lowly inbred Highly inbred Lowly inbred 0 33.17a 34a 32.39a 32.56a 28.07a 27.93a 6.37b 5.51a 1 31.73b 26.13a 36.75a 41.65b 23.80a 24.37a 7.72a 7.85a 2 11.73b 7.67a 53.75b 48.78a 29.68a 39.27b 4.83a 4.28a a,bDifferent superscripts by subpopulation in the same row denote significant difference (P&lt;0.05).

scholarly journals Motion Characteristics and Kinematics of Fresh Spermatozoa of Gir, Surti and Murrah Bulls Assessed By Computer Assisted Semen Analyzer

2018 ◽  
Vol 14 (3) ◽  
Author(s):  
A.J. Dhami ◽  
P.K. Pathak ◽  
D.V. Chaudhari ◽  
K. K. Hadiya
Keyword(s):  
Computer Assisted ◽  
Species Variation ◽  
Sperm Velocity ◽  
Mean Values ◽  
Head Displacement ◽  
Straight Line ◽  
Buffalo Semen ◽  
The Mean ◽  
Motion Characteristics ◽  
Lateral Head

A study was carried out on semen of nine breeding bulls, three each of Gir, Surti and Murrah breeds, to evaluate the comparative motion characteristics and kinematics of their fresh spermatozoa by CASA. The ejaculates (n=72, 24 of each breed) having >75% initial motility were diluted @ 80 million sperm/ml using TFYG extender and were assessed for motion characteristics by CASA. The overall mean values of rapid motile and immotile sperm per cent were observed significantly greater in Gir bulls semen, while total motile and slow motile sperms were apparently higher in buffalo semen. The mean values of sperm velocity/ kinematic parameters observed based on all motile sperms in Gir, Surti and Murrah bulls semen were: average path velocity 50.01±1.25, 48.51±1.03 and 49.14±1.30 μm/s; curvilinear velocity 88.62±1.66, 87.90 ±1.74 amd 88.93±1.69 μm/s, straight line velocity 44.51±1.35, 43.14±1.12 amd 41.73±2.24 μm/s; linearity 50.06±1.42, 48.86±1.32 amd 48.49±1.84 %; straightness 85.17±0.92, 84.97±0.88 and 83.90±1.17 %; wobbling index 57.00±1.17, 55.32±1.05 and 55.30±1.48 %; beat-cross frequency 15.55±0.58, 16.14±0.43 and 14.97±0.54 hz; amplitude of lateral head displacement 2.39±0.18, 2.57±0.12 and 2.31±0.14 μm; dancing frequency 208.34±15.52,225.00 ±10.74 and 211.29±13.03 μm2/s, and dancing mean 5.70±0.46, 6.33±0.35 and 6.33±0.50 μm2/s, respectively. Almost similar trend with little higher values were noted for sperm velocity/kinematics based on only progressively motile sperm, without breed/species variation. The semen of all three breeds behaved identically for sperm kinematics. The bull variation was insignificant for all the traits in all the three breeds.

scholarly journals Quantitative sperm characteristics of Tankwa goats with special reference to hyperactivated motility

2021 ◽  
Vol 50 (5) ◽  
Author(s):  
Ngcauzele Ngcauzele ◽  
G. Van der Horst ◽  
A. Kotze ◽  
T. Jonker ◽  
L. Maree
Keyword(s):  
Sperm Motility ◽  
Animal Species ◽  
Sperm Quality ◽  
Semen Analysis ◽  
Procaine Hydrochloride ◽  
Computer Assisted ◽  
Sperm Analysis ◽  
Sperm Morphometry ◽  
Fertility Potential ◽  
Phosphate Buffered Saline

Computer-assisted semen analysis (CASA) is an automated and objective method of evaluating structural (e.g. morphology) and functional sperm parameters (e.g. motility and hyperactivation). Sperm hyperactivation is essential for successful fertilization and is thus an important aspect in determining the fertility potential of a male. In the current study, CASA was used for standard semen analysis and for comparison of the ability of phosphate buffered saline (PBS), BO sperm wash (10 mM caffeine), 4% lignocaine, and 5 mM procaine hydrochloride to induce hyperactivation in Tankwa goat spermatozoa. Twenty-nine ejaculates were collected from randomly selected male goats by electroejaculation. Although none of the four media affected percentage total sperm motility, lignocaine caused a significant decrease (P >0.05) in percentage progressive motility. Exposure to procaine resulted in an increase in swimming speed (P ≤0.05) and star-spin motility tracks, which are typical of sperm hyperactivation. Using PBS and procaine motility data from individually selected spermatozoa, receiver operator characteristic curves were constructed to distinguish the kinematic parameters employed as cut-off values for sperm hyperactivation. PBS and BO sperm wash did not induce hyperactivation (0.1 + 0.2% and 0.04  0.2% respectively), while lignocaine induced little hyperactivation (3.4 + 3.0%) and procaine hydrochloride had the highest percentage hyperactivation (25.3 + 13.6%). The large variation in hyperactivation (0–54.5%) may reflect inter-individual differences in sperm quality among these males. This study indicated procaine hydrochloride was the most promising hyperactivation-inducing medium for Tankwa goat spermatozoa and should be considered for similar assessments in other animal species Keywords: computer-aided sperm analysis, procaine hydrochloride, sperm kinematics, sperm morphometry, sperm motility

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