Molecular identification of sex in the monomorphic breed of pigeons

In many avian species, especially in monomorphic species and breeds, sex identification creates a serious problem, as they do not show any phenotypic differences. One of such breeds is the Wroclaw Meat Pigeon. In this study, molecular identification of sex with P2 and P8 primers used for the CHD1 (chromo-helicase-DNA-binding-protein) gene amplification was performed. Peripheral blood samples were analyzed from 46 birds, and their DNA was isolated with the phenol-chloroform method. The fragments (370 bp CHD1-Z; 350 bp CHD1-W) obtained from the PCR were cut with the BsuRI. Only the sequence in the Z chromosome was cut into fragments of 305 and 65 bp by the restriction enzyme. The difference between CHD1-Z and CHD1-W was visualized in 3% agarose gel. A single band was identified as male, whereas two bands (plus 1 invisible) were identified as female. Consequently, 23 specimens in each sex were identified.

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New late gene, dar, involved in the replication of bacteriophage T4 DNA. II. Overproduction of DNA binding protein (gene 32 protein) and further characterization.

Journal of Virology ◽

10.1128/jvi.27.1.90-102.1978 ◽

1978 ◽

Vol 27 (1) ◽

pp. 90-102 ◽

Cited By ~ 4

Author(s):

J R Wu ◽

Y C Yeh

Keyword(s):

Dna Binding ◽

Protein Gene ◽

Binding Protein ◽

Bacteriophage T4 ◽

Dna Binding Protein ◽

Late Gene ◽

Binding Protein Gene ◽

Gene 32

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Regulation of Mitochondrial Single-stranded DNA-binding Protein Gene Expression Links Nuclear and Mitochondrial DNA Replication inDrosophila

Journal of Biological Chemistry ◽

10.1074/jbc.275.18.13628 ◽

2000 ◽

Vol 275 (18) ◽

pp. 13628-13636 ◽

Cited By ~ 30

Author(s):

Inmaculada Ruiz de Mena ◽

Etienne Lefai ◽

Rafael Garesse ◽

Laurie S. Kaguni

Keyword(s):

Gene Expression ◽

Mitochondrial Dna ◽

Dna Replication ◽

Dna Binding ◽

Protein Gene ◽

Binding Protein ◽

Dna Binding Protein ◽

Single Stranded Dna ◽

Binding Protein Gene ◽

Mitochondrial Dna Replication

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Nucleotide sequence of the major DNA-binding protein gene of herpes simplex virus type 2 and a comparison with the type 1 counterpart

Archives of Virology ◽

10.1007/bf01316894 ◽

1993 ◽

Vol 129 (1-4) ◽

pp. 183-196 ◽

Cited By ~ 3

Author(s):

Y. Toh ◽

Y. Liu ◽

S. Tanaka ◽

R. Mori

Keyword(s):

Herpes Simplex Virus ◽

Herpes Simplex ◽

Nucleotide Sequence ◽

Herpes Simplex Virus Type ◽

Protein Gene ◽

Dna Binding Protein ◽

Binding Protein Gene ◽

Simplex Virus

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Sequence-specific 1H-NMR assignment and secondary structure of the Tyr41 His mutant of the single-stranded DNA binding protein, gene V protein, encoded by the filamentous bacteriophage M13

European Journal of Biochemistry ◽

10.1111/j.1432-1033.1991.tb16382.x ◽

1991 ◽

Vol 202 (2) ◽

pp. 349-360 ◽

Cited By ~ 26

Author(s):

Paul J. M. FOLKERS ◽

John P. M. DUYNHOVEN ◽

Aafke J. JONKER ◽

Ben J. M. HARMSEN ◽

Ruud N. H. KONINGS ◽

...

Keyword(s):

Secondary Structure ◽

Dna Binding ◽

1H Nmr ◽

Nmr Assignment ◽

Protein Gene ◽

Dna Binding Protein ◽

Filamentous Bacteriophage ◽

V Protein ◽

Bacteriophage M13 ◽

Binding Protein Gene

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A simple and reliable molecular method for sex identification of the Brown-eared pheasant (Crossoptilon mantchuricum) from non-invasively collected samples

Animal Biology ◽

10.1163/157075511x566498 ◽

2011 ◽

Vol 61 (2) ◽

pp. 163-173 ◽

Cited By ~ 1

Author(s):

Qiuhua Zhang ◽

Chungui Zhao ◽

Mengben Wang ◽

Feng Zhang ◽

Yuzhen Wu ◽

...

Keyword(s):

Sex Identification ◽

Agarose Gel ◽

Wild Populations ◽

Molecular Method ◽

Non Invasive ◽

Endemic Birds ◽

Captive Populations ◽

Invasive Method ◽

The Difference ◽

First Time

AbstractFor sex identification of the Brown-eared pheasant (Crossoptilon mantchuricum), one of the critically endangered endemic birds in China, the morphological method of checking the astragalus, an extra tiny bone on the ankle only of male ones is inconvenient and even impossible for wild populations. In this paper, we investigated a simple reliable non-invasive method according to the difference of the sizes of sex-linked genes CHD1-W and CHD1-Z (Griffiths et al., 1996; Ellegren, 1996) to identify the gender of individuals in two captive populations of the Brown-eared pheasant. We extracted DNA from blood and feather samples and amplified the genes by PCR using two pairs of primers P2/P8 (Griffiths et al., 1998) and 2550F/2718R (Fridolfsson et al., 1999). The products amplified with P2/P8 failed to show the sex due to the low resolution of the agarose gel. PCR using the 2550F/2718R primer set amplified two products of different sizes for all known females and a single product for all known males when scored on the 2.0% agarose gel, which indicated that this primer set enabled sex identification. Both blood and feather samples gave identical results although the products amplified from the feather samples were fewer than the blood samples which were taken invasively and acted as control. This is the first time molecular methods was used for sex identifications of the Brown-eared pheasant and will assist their management by means of artificial propagation and allow the study of the ecology and conservation genetics of the Brown-eared pheasant.

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