Genetic improvement of two cultivars of Vicia faba L. using gamma irradiation and ethyl methanesulphonate mutagenesis

2016 ◽  
Author(s):  
Shahnawaz Khursheed ◽  
Samiullah Khan
Keyword(s):  
Gamma Irradiation ◽  
Protein Content ◽  
Vicia Faba ◽  
Mineral Content ◽  
Sodium Dodecyl ◽  
Ethyl Methanesulphonate ◽  
Mineral Contents ◽  
Sds Page ◽  
Vicia Faba L ◽  
Mutant Lines

The present experiment was conducted to induce genetic variation using gamma irradiation and ethyl methanesulphonate mutagenesis and isolate and analyze the desirable mutant lines of Vicia faba L. var. Vikrant and PRT-12 in the M3 generation. The seeds of the mutant lines isolated from 3rd generation plants were analyzed for different parameters including total protein content, protein profiles and mineral content using Lowry method, Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and atomic absorption spectrophotometer, respectively. SDS-PAGE profile of mutants from variety Vikrant showed more polymorphism and expression of proteins than mutants isolated from variety PRT-12. Mutant seeds showed significantly higher protein content (0.18 to 1.29%) and mineral content (0.21 to 2.98 mg.g-1) compared to the controls. The mutants selected from variety Vikrant showed more increase in protein and mineral contents than the mutants selected from PRT-12. The selected mutant lines may serve as a valuable genetic material to breed for the improvement of protein and mineral contents in faba bean and other valuable crop plants.

Peculiarities of extraction of β-lactoglobuline in protein mineral concentrates at electroactivation of whey

2020 ◽  
Vol 2 (1) ◽  
pp. 52-68
Author(s):  
Mircea BOLOGA ◽  
Elvira VRABIE ◽  
Irina PALADII ◽  
Olga ILIASENCO ◽  
Tatiana STEPURINA ◽  
...  
Keyword(s):  
Protein Content ◽  
Whey Proteins ◽  
Sodium Dodecyl ◽  
Total Protein Content ◽  
Treatment Regimens ◽  
Sds Page ◽  
Different Types ◽  
Lower Protein ◽  
Β Lactoglobulin ◽  
Excellent Source

Introduction. Whey is a by-product and an excellent source of proteins that is rather aggressive due to a large amount of organic substances it contains. The electro-activation of whey applied in the experiments is a wasteless method that allows the va-lorification of all whey components. β-lactoglobulin (β-Lg) makes up 50% of the whey proteins and 12% of the total protein content in milk. Material and methods. The recovery of β-Lg in protein-mineral concentrates (PMC) by electro-activation processing of different types of whey with different initial protein content was investigated in seven configurations. The recovery of protein fractions in the PMCs were analyzed via electrophoresis with sodium dodecyl sulfate (SDS-PAGE) and 15% non-denaturing polyacrylamide gel (PAAG).      Results. Whey electro-fractionation and the obtaining of PMCs with predetermined protein content, namely of β-Lg, were studied on three whey types, processed at different treatment regimens and in seven configurations. The proper management of electroactivation by varying the treatment regimens will allow the electro-fractionation of different types of dairy by-products. Conclusions. The maximum amount of β-Lg recovered in PMCs on electroactivation is  66-71% depending on the processed whey and on the treatment regimens. Obviously, the extraction of β-Lg from initially lower protein content shows a higher recovery degree of β-Lg. The registered temperatures allows formation of PMCs without thermal denaturation.

Effect of sodium azide on Bambara groundnut (Vigna subterranean (L.) verdc.) as revealed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page)

2021 ◽  
Vol 20 (1) ◽  
pp. 183-194
Author(s):  
Odunayo Joseph Olawuyi ◽  
Juliet Ese Naworu ◽  
Roseline Tolulope Feyisola
Keyword(s):  
Protein Content ◽  
Sodium Azide ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Lethal Effect ◽  
Bambara Groundnut ◽  
International Institute ◽  
Yield Traits ◽  
North East ◽  
Sds Page

This study investigated the mutagenic effects of Sodium Azide (NaN3) on the agromorphological and protein content of eight Bambara groundnut genotypes. The seeds of six genotypes; TVSu-86, TVSu-91, TVSu-186, TVSu-235, TVSu-242, TVSu-350 were collected fromthe International Institute of Tropical Agriculture (IITA) and two landraces from Abia State and Enugu State North East, Nigeria local markets. The seeds were treated with five concentrations: 0.00%(control), 0.01%, 0.03%, 0.05% and 0.07% of NaN3 after pre-soakingfor 6hrs in distilled water and sown in pots arranged in a Complete Randomized Design with three replicates. There was reduction in germination percentage and growth characters as concentrations of NaN3 increases. Early flowering was recorded at 37 days mutated with 0.07% of NaN3 compared to control which flowered late at 42 days. NaN3 (0.07%) caused lethal effect on Abia and Enugu landraces. There was no significant (P>0.05) difference in yield traits among mutants and control. Mutant seeds significantly (P<0.05) increased protein content (19.12%) at 0.05% of NaN3 compared to control (18.5%). The number of seeds (0.99), seed yield (0.89) and pod yield (0.96) strongly correlated with seeds per pod (0.85). The SDS-PAGE revealed the presence of polypeptide bands in mutants compared to control. TVSu-235 and TVSu-350 genotypes had higher tolerance and yield traits to 0.01% concentration of NaN3, thuscould be further improved in subsequent breeding. Keywords: Bambara groundnut, Sodium azide, SDS-PAGE, polypeptide bands.

RESPONSE OF SMALL FABABEANS TO SEED RATE AND SPACING

1973 ◽  
Vol 53 (2) ◽  
pp. 279-283 ◽  
Author(s):  
J. F. SEITZER ◽  
L. E. EVANS
Keyword(s):  
Protein Content ◽  
Vicia Faba ◽  
Seed Yield ◽  
Seed Weight ◽  
Unit Area ◽  
Yield Component ◽  
Direct Function ◽  
Seed Rate ◽  
Wide Spacing ◽  
Vicia Faba L

Small fababeans (Vicia faba L.) were tested at one location for 2 yr at six seed rates (60–260 kg/ha) and two row spacings (15 and 30 cm). Results indicate that narrow spacing outyields wide spacing at all seed rates, the average advantage being 18.9 q/ha and 5.0 q/ha for 1971 and 1972, respectively. Seed yield increased with seed rate; highest yields were obtained at a seed rate of 260 kg/ha and 15 cm spacing. Treatments had no significant effects on 1,000-seed weight hectoliter weight, or protein content. Yield component analysis revealed that the number of pods per plant decreased with increased seed rates, but weight per pod was not affected. It is concluded that seed yield is a direct function of the number of mature pods per unit area.

scholarly journals Variation of some seed mineral contents in open pollinated faba bean (Vicia faba L.) landraces from Turkey

2014 ◽  
Vol 38 ◽  
pp. 591-602 ◽  
Author(s):  
Faheem Shehzad BALOCH ◽  
Tolga KARAKÖY ◽  
Ahmet DEMİRBAŞ ◽  
Faruk TOKLU ◽  
Hakan ÖZKAN ◽  
...  
Keyword(s):  
Vicia Faba ◽  
Faba Bean ◽  
Mineral Contents ◽  
Vicia Faba L

Genetic variability for protein content and S-aminoacids in broad-beans (Vicia faba L.)

1981 ◽  
Vol 29 (1) ◽  
pp. 115-127 ◽  
Author(s):  
Domenico Lafiandra ◽  
Gian Battista Polignano ◽  
Andrea Filippetti ◽  
Enrico Porceddu
Keyword(s):  
Genetic Variability ◽  
Protein Content ◽  
Vicia Faba ◽  
Broad Beans ◽  
Vicia Faba L
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