Biofilm Formation Enhances the Oxygen Tolerance and Invasiveness ofFusobacterium nucleatumin an Oral Mucosa Culture Model

2010 ◽  
Vol 81 (7) ◽  
pp. 1084-1091 ◽  
Author(s):  
Ulvi Kahraman Gursoy ◽  
Marja Pöllänen ◽  
Eija Könönen ◽  
Veli-Jukka Uitto
Keyword(s):  
Oral Mucosa ◽  
Biofilm Formation ◽  
Oxygen Tolerance ◽  
Culture Model

Camphorquinone alters the expression of extracellular proteases in a 3D co-culture model of the oral mucosa

2020 ◽  
Author(s):  
Renke Perduns ◽  
Joachim Volk ◽  
Melanie Plum ◽  
André Jochums ◽  
Frank Gutzki ◽  
...  
Keyword(s):  
Oral Mucosa ◽  
Extracellular Proteases ◽  
Culture Model

scholarly journals Synergistic Interaction between Candida albicans and Commensal Oral Streptococci in a NovelIn VitroMucosal Model

2011 ◽  
Vol 80 (2) ◽  
pp. 620-632 ◽  
Author(s):  
Patricia I. Diaz ◽  
Zhihong Xie ◽  
Takanori Sobue ◽  
Angela Thompson ◽  
Basak Biyikoglu ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Gastrointestinal Tract ◽  
Oral Mucosa ◽  
Biofilm Formation ◽  
Salivary Flow ◽  
Flow Cell ◽  
Synergistic Interaction ◽  
Esophageal Mucosa ◽  
Oral Streptococci ◽  
Content Type

ABSTRACTCandida albicansis a commensal colonizer of the gastrointestinal tract of humans, where it coexists with highly diverse bacterial communities. It is not clear whether this interaction limits or promotes the potential ofC. albicansto become an opportunistic pathogen. Here we investigate the interaction betweenC. albicansand three species of streptococci from the viridans group, which are ubiquitous and abundant oral commensal bacteria. The ability ofC. albicansto form biofilms withStreptococcus oralis,Streptococcus sanguinis, orStreptococcus gordoniiwas investigated using flow cell devices that allow abiotic biofilm formation under salivary flow. In addition, we designed a novel flow cell system that allows mucosal biofilm formation under conditions that mimic the environment in the oral and esophageal mucosae. It was observed thatC. albicansand streptococci formed a synergistic partnership whereC. albicanspromoted the ability of streptococci to form biofilms on abiotic surfaces or on the surface of an oral mucosa analogue. The increased ability of streptococci to form biofilms in the presence ofC. albicanscould not be explained by a growth-stimulatory effect since the streptococci were unaffected in their growth in planktonic coculture withC. albicans. Conversely, the presence of streptococci increased the ability ofC. albicansto invade organotypic models of the oral and esophageal mucosae under conditions of salivary flow. Moreover, characterization of mucosal invasion by the biofilm microorganisms suggested that the esophageal mucosa is more permissive to invasion than the oral mucosa. In summary,C. albicansand commensal oral streptococci display a synergistic interaction with implications for the pathogenic potential ofC. albicansin the upper gastrointestinal tract.

Effects of tacrolimus on an organotypic raft-culture model mimicking oral mucosa

2012 ◽  
Vol 37 (8) ◽  
pp. 897-903 ◽  
Author(s):  
J. Rautava ◽  
M. Pöllänen ◽  
M. A. Laine ◽  
J. Willberg ◽  
H. Lukkarinen ◽  
...  
Keyword(s):  
Oral Mucosa ◽  
Raft Culture ◽  
Culture Model

Effects of HEMA on Nrf2-related gene expression using a newly developed 3D co-culture model of the oral mucosa

2019 ◽  
Vol 35 (9) ◽  
pp. 1214-1226 ◽  
Author(s):  
Renke Perduns ◽  
Joachim Volk ◽  
Melanie Plum ◽  
Frank Gutzki ◽  
Volkhard Kaever ◽  
...  
Keyword(s):  
Gene Expression ◽  
Oral Mucosa ◽  
Related Gene ◽  
Culture Model

scholarly journals Clinical significance of the level of intercellular adhesion molecules 1 in patients with Candida stomatitis depending on the ability of Candida spp to foms biofilms

2020 ◽  
pp. 52-57
Author(s):  
D Novikov ◽  
A Pozharitskaya ◽  
I Karpuk
Keyword(s):  
Adhesion Molecules ◽  
Oral Mucosa ◽  
Biofilm Formation ◽  
Oral Fluid ◽  
Oral Candidiasis ◽  
Intercellular Adhesion ◽  
Control Group ◽  
Microbiological Method ◽  
Candida Spp ◽  
Intercellular Adhesion Molecules

Aim: to assess the level of intercellular adhesion molecules 1 (ICAM-1) in the oral fluid of patients with candidal stomatitis, depending on the ability of fungi Candida to form a biofilm. The object of the study were 67 patients with oral mucosa candidiasis and 23 patients of the control group without oral candidiasis. A clinical examination of 90 patients was carried out, smears were taken to confirm the diagnosis by a microbiological method, ELISA to assess the level of ICAM-1 in the oral fluid, to determine the biofilm-forming ability of strains of fungi Candida. According to the results of the study, in 41 (61,2%) patients with candidal stomatitis, strains of fungi Candida had the ability to form a biofilm, and in 26 (38,8%) patients this ability was absent, and in 19 patients (46,3%) it was low ability to biofilm formation, in 21 (51,3%) - moderate ability and in 1 (2,4%) - high. In patients with candidiasis stomatitis, the level of ICAM-1 concentration in saliva was 8,51 ± 0,5 ng / ml, which significantly distinguished it (p <0,001) from the indicator in the control group without oral mucosa candidiasis (4,51 ± 0,32 ng / ml). In patients with candidal stomatitis (n = 67), the level of ICAM-1 in saliva was significantly higher (p <0,01) in the group with biofilm-forming strains than with biofilm-non-forming strains.

scholarly journals Evaluation of Absorbability of Macromolecular Substances in the Oral Mucosa and Skin using a Three-Dimensional Tissue Culture Model

2018 ◽  
Vol 10 (5) ◽  
Author(s):  
Mariko Takenokuchi ◽  
Keiichi Kadoyama ◽  
Daisuke Yoshida ◽  
Shigeki Takaki ◽  
Ryoma Yamamoto ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Oral Mucosa ◽  
Three Dimensional ◽  
Culture Model ◽  
Tissue Culture Model

scholarly journals The Effects of Nonnutritive Sweeteners on the Cariogenic Potential of Oral Microbiome

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Jianhui Zhu ◽  
Jiaxin Liu ◽  
Zhengyi Li ◽  
Ranhui Xi ◽  
Yuqing Li ◽  
...  
Keyword(s):  
Dental Caries ◽  
Biofilm Formation ◽  
Ph Value ◽  
Single Species ◽  
Oral Microbiome ◽  
Sugar Consumption ◽  
Culture Model ◽  
Frequent Exposure ◽  
Multispecies Biofilms

Nonnutritive sweeteners (NNSs) are sugar substitutes widely used to reduce the negative health effects of excessive sugar consumption. Dental caries, one of the most prevalent chronic diseases globally, results from a pathogenic biofilm with microecological imbalance and frequent exposure to sugars. Some research has shown that certain NNSs possess less cariogenic potential than sucrose, indicating their putative effect on oral microbiome. To uncover the alterations of acidogenic pathogens and alkali-generating commensals, as well as the biofilm cariogenic potential under the influence of NNSs, we selected four common NNSs (acesulfame-K, aspartame, saccharin, and sucralose) and established single-, dual-, and multispecies in vitro culture model to assess their effects on Streptococcus mutans (S. mutans) and/or Streptococcus sanguinis (S. sanguinis) compared to sucrose with the same sweetness. The results showed that NNSs significantly suppressed the planktonic growth, acid production, and biofilm formation of S. mutans or S. sanguinis compared with sucrose in single-species cultures. Additionally, decreased S. mutans/S. sanguinis ratio, less EPS generation, and higher pH value were observed in dual-species and saliva-derived multispecies biofilms with supplementary NNSs. Collectively, this study demonstrates that NNSs inhibit the cariogenic potential of biofilms by maintaining microbial equilibrium, thus having a promising prospect as anticaries agents.

Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

1982 ◽  
Vol 40 ◽  
pp. 142-145
Author(s):  
E. J. Kollar
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Basal Lamina ◽  
Functional Derivatives ◽  
Specific Source ◽  
Dental Epithelium ◽  
Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

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