scholarly journals Clinical significance of the level of intercellular adhesion molecules 1 in patients with Candida stomatitis depending on the ability of Candida spp to foms biofilms

2020 ◽  
pp. 52-57
Author(s):  
D Novikov ◽  
A Pozharitskaya ◽  
I Karpuk
Keyword(s):  
Adhesion Molecules ◽  
Oral Mucosa ◽  
Biofilm Formation ◽  
Oral Fluid ◽  
Oral Candidiasis ◽  
Intercellular Adhesion ◽  
Control Group ◽  
Microbiological Method ◽  
Candida Spp ◽  
Intercellular Adhesion Molecules

Aim: to assess the level of intercellular adhesion molecules 1 (ICAM-1) in the oral fluid of patients with candidal stomatitis, depending on the ability of fungi Candida to form a biofilm. The object of the study were 67 patients with oral mucosa candidiasis and 23 patients of the control group without oral candidiasis. A clinical examination of 90 patients was carried out, smears were taken to confirm the diagnosis by a microbiological method, ELISA to assess the level of ICAM-1 in the oral fluid, to determine the biofilm-forming ability of strains of fungi Candida. According to the results of the study, in 41 (61,2%) patients with candidal stomatitis, strains of fungi Candida had the ability to form a biofilm, and in 26 (38,8%) patients this ability was absent, and in 19 patients (46,3%) it was low ability to biofilm formation, in 21 (51,3%) - moderate ability and in 1 (2,4%) - high. In patients with candidiasis stomatitis, the level of ICAM-1 concentration in saliva was 8,51 ± 0,5 ng / ml, which significantly distinguished it (p <0,001) from the indicator in the control group without oral mucosa candidiasis (4,51 ± 0,32 ng / ml). In patients with candidal stomatitis (n = 67), the level of ICAM-1 in saliva was significantly higher (p <0,01) in the group with biofilm-forming strains than with biofilm-non-forming strains.

scholarly journals Pathogenetic Relationship between Endothelial Dysfunction and Disorders of Blood Coagulation Potential in Pregnancy Complicated by Pre-Eclampsia

2015 ◽  
Vol 70 (5) ◽  
pp. 599-603 ◽  
Author(s):  
O. N. Sergeeva ◽  
N. P. Chesnokova ◽  
E. V. Ponukalina ◽  
I. E. Rogozhina ◽  
T. N. Glukhova
Keyword(s):  
Nitric Oxide ◽  
Endothelial Dysfunction ◽  
Blood Plasma ◽  
Blood Coagulation ◽  
Adhesion Molecules ◽  
Intercellular Adhesion ◽  
Control Group ◽  
Endothelin 1 ◽  
Intercellular Adhesion Molecules ◽  
Nitric Oxide Metabolites

Until now, there is no systematic information on the role of endothelial dysfunction in the mechanisms of disorders of blood coagulation potential and microcirculation in different organs and tissues in preeclampsia.Objective: Our aim was to extend the existing principles of diagnosis of pre-eclampsia by establishing the role of endothelial dysfunction in the mechanisms of blood coagulation potential violations. Methods: A prospective comparative study was performed. Condition of coagulation processes studied by conventional techniques, parameters of a functional endothelium (nitric oxide metabolites, endothelin 1, thrombospondin, thrombomodulin and intercellular adhesion molecules in blood plasma) — by ELISA.Results: The study group included 55 patients with moderate preeclampsia and 49 pregnant women with severe pre-eclampsia, in the control group — 40 women with physiological pregnancy. In patients with pre-eclampsia moderate observed increase in plasma endothelin-1 (p 0.001), thrombospondin (p 0.001), intercellular adhesion molecules (p 0.001) while reducing the level of nitrogen oxide (p 0.001), increase in time of fibrinolysis (p 0.050) and decreased international normalized ratio (p 0.050) compared with the control group. With increasing severity of preeclampsia the researchers detected in blood plasma of patients a progressive increase in endothelin 1 (p1 0.020), thrombospondin (p1 0.001), intercellular adhesion molecules (p1 0.001) and decrease of nitric oxide metabolites (p1 0.001) and thrombomodulin (p1 0.001); the last combined with the activation of procoagulant hemostasis.Conclusion: There is a pathogenetic relationship between the development of endothelial dysfunction, impaired blood coagulation potential and the severity of clinical signs of preeclampsia. To widen the number of existing techniques to diagnose the severity of pre-eclampsia we recommende to mesure endothelin 1, thrombomodulin, thrombospondin, intercellular adhesion molecules and nitric oxide metabolites in the blood plasma, and use traditional indicators to assess the hemostatic system.

scholarly journals The relationship of the level of β-defensin-1 in the saliva of patients with oral candidiasis after CoVID-19 infection

2021 ◽  
pp. 97-102
Author(s):  
A. Pozharitskaya ◽  
I. Karpuk
Keyword(s):  
Epithelial Cells ◽  
Oral Mucosa ◽  
Oral Candidiasis ◽  
Control Group ◽  
Microbiological Method ◽  
Objective Indicator ◽  
History Of ◽  
Coronavirus Infection ◽  
Relationship Of ◽  
The Relationship

Aim: to determine the level and clinical significance of β-defensin-1 in the saliva in patients with Candida stomatitis after a previous coronavirus infection. The object of the study was 67 patients with Candida stomatitis (of which 31 patients had a PCR-confirmed diagnosis of coronavirus infection) and 23 patients of the control group without candidal stomatitis. Research methods: taking smears from the oral mucosa to confirm the diagnosis with a microbiological method, ELISA to assess the level of β-defensin 1 in the saliva. Аccording to the results of the study, in the group of patients with oral candidiasis (ОС) after COVID-19 (n=31), the most frequent was the chronic hyperplastic form of candidiasis (64,5%), in terms of localization – Candida glossitis (77,4%, p<0,05). It was found that the primary ОС in the group of patients after COVID-19 (n=31) was 74,2% of cases (p<0,05), and in the group of patients with ОС without a history of COVID-19 (n=36) this indicator amounted to 41,7%. Moreover, in patients in the group with ОС after COVID-19, the level of β-defensin-1 strongly positively correlated with the incidence of primary episodes of ОС (RSpearman=0,76, p<0,001). It was also found that the lowest β-defensin-1 level in saliva was in patients with ОС after COVID-19 (2,1±0,8 ng/ml, p<0,001). It was significantly lower (p<0,001) than in the group of patients with ОС and the control group. It was found that in patients with ОС after COVID-19, the level of β-defensin-1 in the saliva was negatively correlated with the duration of the COVID-19 (RSpearman=- 0,78, p<0,001), which can be interpreted as an objective indicator of the effect of SARS-CoV-2 on epithelial cells of the oral mucosa.

scholarly journals Evaluation of the Effectiveness of Crotoxin as an Antiseptic against Candida spp. Biofilms

Toxins ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 532
Author(s):  
Amanda Pissinatti Canelli ◽  
Taís Fernanda dos Santos Rodrigues ◽  
Vivian Fernandes Furletti de Goes ◽  
Guilherme Ferreira Caetano ◽  
Maurício Ventura Mazzi
Keyword(s):  
Biofilm Formation ◽  
Colorimetric Assay ◽  
Oral Candidiasis ◽  
Hacat Cells ◽  
Candida Spp ◽  
Oral Infections ◽  
Minimum Fungicidal Concentration ◽  
Antimicrobial Susceptibility Tests ◽  
Susceptibility Tests

The growing number of oral infections caused by the Candida species are becoming harder to treat as the commonly used antibiotics become less effective. This drawback has led to the search for alternative strategies of treatment, which include the use of antifungal molecules derived from natural products. Herein, crotoxin (CTX), the main toxin of Crotalus durissus terrificus venom, was challenged against Candida tropicalis (CBS94) and Candida dubliniensis (CBS7987) strains by in vitro antimicrobial susceptibility tests. Minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC), and inhibition of biofilm formation were evaluated after CTX treatment. In addition, CTX-induced cytotoxicity in HaCaT cells was assessed by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) colorimetric assay. Native CTX showed a higher antimicrobial activity (MIC = 47 μg/mL) when compared to CTX-containing mouthwash (MIC = 750 μg/mL) and nystatin (MIC = 375 μg/mL). Candida spp biofilm formation was more sensitive to both CTX and CTX-containing mouthwash (IC100 = 12 μg/mL) when compared to nystatin (IC100 > 47 μg/mL). Moreover, significant membrane permeabilization at concentrations of 1.5 and 47 µg/mL was observed. Native CTX was less cytotoxic to HaCaT cells than CTX-containing mouthwash or nystatin between 24 and 48 h. These preliminary findings highlight the potential use of CTX in the treatment of oral candidiasis caused by resistant strains.

Intercellular adhesion molecules in systemic lupus erythematosus patients with lupus nephritis

2007 ◽  
Vol 26 (11) ◽  
pp. 1819-1823 ◽  
Author(s):  
Alaa Sabry ◽  
Hussein Sheashaa ◽  
Amr El-Husseini ◽  
Khaled El-Dahshan ◽  
Mona Abdel-Rahim ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Nephritis ◽  
Adhesion Molecules ◽  
Lupus Erythematosus ◽  
Intercellular Adhesion ◽  
Systemic Lupus ◽  
Intercellular Adhesion Molecules

scholarly journals Differential regulation of leukocyte function-associated antigen-1/ intercellular adhesion molecules-1-dependent adhesion and aggregation in HL-60 cells

Blood ◽  
1996 ◽  
Vol 87 (10) ◽  
pp. 4276-4285 ◽  
Author(s):  
K Katagiri ◽  
T Kinashi ◽  
S Irie ◽  
T Katagiri
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecules ◽  
Transforming Growth Factor ◽  
De Novo ◽  
Active Form ◽  
Intercellular Adhesion ◽  
Cytoskeletal Proteins ◽  
Leukocyte Function ◽  
Cellular Aggregation ◽  
Intercellular Adhesion Molecules

Activation of integrin and organization of cytoskeletal proteins are highly regulated in cell adhesion and aggregation. The interaction of leukocyte function-associated antigen-1 (LFA-1) and intercellular adhesion molecules-1 (ICAM-1) mediates cell adhesion and aggregation, which facilitate leukocyte trafficking to inflamed tissues and augment effector functions. We investigated how LFA-1/ICAM-1-mediated adhesion and aggregation are regulated in HL-60 cells induced to differentiate into neutrophils by retinoic acid (RA). Uninduced HL-60 cells did not bind to ICAM-1 even with stimulation by 12–0-tetradecanoyl phorbol-13- acetate, although they express LFA-1 on the cell surface. When cultured with RA for 24 hours, HL-60 cells were able to adhere to ICAM-1 constitutively. The induction of adhesion did not accompany any change in surface density of LFA-1, indicating that the avidity of LFA-1 was increased. The change in its avidity required de novo synthesis of proteins. Although ICAM-1 was intensely expressed on RA-induced HL-60 cells, these cells did not show any cellular aggregation. The HL-60 cells transfected with the active form of Ras (Val12) exhibited LFA- 1/ICAM-1-dependent aggregation by RA stimulation without change in the avidity of LFA-1. In these Ras-transfectants, a cytoskeletal protein, paxillin, was tyrosine-phosphorylated, and the level of F-actin increased. Transforming growth factor (TGF) beta, as well as cytochalasin D, prevented both the tyrosine phosphorylation of paxillin and the aggregation without any effects on the avidity of LFA-1. Thus, an increase in the avidity of LFA-1 was not sufficient for the induction of aggregation, which required activation of Ras and reorganization of cytoskeletal proteins. These results suggest that distinct regulatory mechanisms control LFA-1/ICAM-1-dependent adhesion and aggregation in HL-60 cells differentiating into neutrophils.

Soluble Intercellular Adhesion Molecules, Soluble Vascular Cell Adhesion Molecules, and Risk of Coronary Heart Disease*

Obesity ◽  
2006 ◽  
Vol 14 (11) ◽  
pp. 2099-2106 ◽  
Author(s):  
Iris Shai ◽  
Tobias Pischon ◽  
Frank B. Hu ◽  
Alberto Ascherio ◽  
Nader Rifai ◽  
...  
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Cell Adhesion ◽  
Adhesion Molecules ◽  
Cell Adhesion Molecules ◽  
Intercellular Adhesion ◽  
Vascular Cell Adhesion ◽  
Vascular Cell ◽  
Intercellular Adhesion Molecules
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