The Expression of Toll-like Receptor (TLR)4 of Immune Cells In The Saliva Associated with Serum 25-hydroxy vitamin D/25(OH)D in Systemic Lupus Erythematosus: A Pilot Study

Objective: Vitamin D deficiency may contribute to Systemic Lupus Erythematosus (SLE) development. Vitamin D may involve in pathogen recognition through Toll-like receptor (TLR) 2 in immune cells in saliva. This study aimed to determine the correlation between serum vitamin D/25(OH)D and TLR2 expression of immune cells in the saliva of SLE. Materials and methods: This cross-sectional study conducted at the the SLE patients who met the inclusion and exclusion criteria. Those who had signed informed consent involved to underwent unstimulated saliva collection and blood samples for TLR2 and vitamin D/25(OH)D examination. The correlation between serum vitamin D/25(OH)D concentration and salivary TLR2 expression was analyzed using the correlation test, linear regression with 95% confidence level. Results: Thirty SLE patients had a mean serum vitamin D/25(OH)D concentration of 9.98 ± 4.64 ng/ml. The mean of TLR2 expression of CD11b+ cells in saliva was 26.03 ± 20.92%. There was a significant positive correlation between serum vitamin D/25(OH)D concentration and TLR 2 expression of CD11b+ cells in the saliva. (r = 0.434; P < 0.05). Vitamin D/25(OH)D was the only predictor for TLR 2 expression. Conclusion: Serum vitamin D/25(OH)D concentrations associated with TLR2 expression of CD11b+ cells in the saliva of SLE.

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25-Hydroxy vitamin D levels and its relation to disease activity and cardiovascular risk factors in women with systemic lupus erythematosus

The Egyptian Rheumatologist ◽

10.1016/j.ejr.2011.07.004 ◽

2011 ◽

Vol 33 (4) ◽

pp. 195-201 ◽

Cited By ~ 5

Author(s):

Yasser Ezzat ◽

Safaa Sayed ◽

Wafaa Gaber ◽

Abeer M. Mohey ◽

Tamer Wahid Kassem

Keyword(s):

Risk Factors ◽

Systemic Lupus Erythematosus ◽

Vitamin D ◽

Cardiovascular Risk ◽

Disease Activity ◽

Cardiovascular Risk Factors ◽

Lupus Erythematosus ◽

Systemic Lupus ◽

Vitamin D Levels ◽

25 Hydroxy Vitamin D

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The Relationship Between Serum Vitamin D Level and Systemic Lupus Erythematosus Activity

International journal of epidemiologic research ◽

10.34172/ijer.2020.01 ◽

2020 ◽

Vol 7 (1) ◽

pp. 1-5 ◽

Cited By ~ 1

Author(s):

Batool Zamani ◽

Hossein Akbari ◽

Mehrdad Mahdian ◽

Ehsan Dadgostar

Keyword(s):

Systemic Lupus Erythematosus ◽

Vitamin D ◽

Lupus Erythematosus ◽

Healthy Subjects ◽

Linear Regression Analysis ◽

Serum Vitamin ◽

Control Group ◽

Systemic Lupus ◽

25 Hydroxy Vitamin D ◽

The Relationship

Background and aims: : Systemic lupus erythematosus (SLE) is an autoimmune disease which involves various organs. Vitamin D is an essential ingredient in regulating the immune system. This study aimed to investigate the relationship between vitamin D and the severity of lupus activity. Materials and Methods: This case-control study was carried out on 38 patients with lupus on the basis of the American College of Rheumatology (ACR) criteria and 44 healthy subjects with no history of rheumatologic disease. To measure the level of 25-hydroxy vitamin D, venous blood samples (5 cc) were taken from each participant and the activity of the lupus disease was measured by the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) scale. Finally, the chi-square test, independent sample t test, one-way ANOVA, and multiple linear regression analysis were used to measure multivariate effects. The level of significance was set to be P<0.05. Results: Thirty-five lupus patients and 40 healthy subjects were females (P=0.847). Vitamin D deficiency was observed in the case (42.1%) and control (11.4%) groups. The mean value of serum vitamin D3 level was 35.3 ng/mL in the control group, as well as 24.6 ng/mL and 21.3 ng/mL in patients with mild and severe SLE, respectively (P=0.024). Conclusion: In this study, high levels of 25-hydroxy vitamin D were observed among the healthy subjects compared to patients with SLE. Eventually, the level of vitamin D significantly decreased by increasing the severity of SLE activity.

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Reduced serum concentrations of 25-hydroxy vitamin D in Egyptian patients with systemic lupus erythematosus: Relation to disease activity

Medical Science Monitor ◽

10.12659/msm.882131 ◽

2011 ◽

Vol 17 (12) ◽

pp. CR711-CR718 ◽

Cited By ~ 42

Author(s):

Rasha T. Hamza ◽

Khaled S. Awwad ◽

Mohamed K. Ali ◽

Amira I. Hamed

Keyword(s):

Systemic Lupus Erythematosus ◽

Vitamin D ◽

Disease Activity ◽

Lupus Erythematosus ◽

Serum Concentrations ◽

Systemic Lupus ◽

Egyptian Patients ◽

25 Hydroxy Vitamin D

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1,25-(OH)2D3/Vitamin D receptor alleviates systemic lupus erythematosus by downregulating Skp2 and upregulating p27

Cell Communication and Signaling ◽

10.1186/s12964-019-0488-2 ◽

2019 ◽

Vol 17 (1) ◽

Cited By ~ 1

Author(s):

Dan Liu ◽

Yu-Xuan Fang ◽

Xia Wu ◽

Wei Tan ◽

Wei Zhou ◽

...

Keyword(s):

Systemic Lupus Erythematosus ◽

Vitamin D ◽

Vitamin D Receptor ◽

Immune Cells ◽

Lupus Erythematosus ◽

Inflammatory Responses ◽

Urinary Protein ◽

Inflammatory Factors ◽

Systemic Lupus ◽

Protein Levels

Abstract Background Recent evidence has suggested that the 1,25(OH)2D3/Vitamin D receptor (VDR) acts to suppress the immune response associated with systemic lupus erythematosus (SLE), a serious multisystem autoimmune disease. Hence, the aim of the current study was to investigate the mechanism by which 1,25-(OH)2D3/VDR influences SLE through regulating the Skp2/p27 signaling pathway. Methods Initially, the levels of 1,25(OH)2D3, VDR, Skp2, and p27 were measured in collected renal tissues and peripheral blood. Meanwhile, the levels of inflammatory factors, biochemical indicators (BUN, Cr, anti-nRNP IgG, anti-dsDNA IgG) and urinary protein levels were assayed in in VDRinsert and VDR-knockout mice in response to 1,25(OH)2D3 supplement. In addition, the distribution of splenic immune cells was observed in these mice. Results Among the SLE patients, the levels of 1,25(OH)2D3, VDR and p27 were reduced, while the levels of Skp2 were elevated. In addition, the levels of anti-nRNP IgG and anti-dsDNA IgG were increased, suggesting induction of inflammatory responses. Notably, 1,25(OH)2D3/VDR mice had lower concentrations of BUN and Cr, urinary protein levels, precipitation intensity of the immune complex and complement, as well as the levels of anti-nRNP IgG and anti-dsDNA IgG in SLE mice. Additionally, 1,25(OH)2D3 or VDR reduced the degree of the inflammatory response while acting to regulate the distribution of splenic immune cells. Conclusion This study indicated that 1,25-(OH)2D3/VDR facilitated the recovery of SLE by downregulating Skp2 and upregulating p27 expression, suggesting the potential of 1,25-(OH)2D3/VDR as a promising target for SLE treatment.

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Bone turnover markers in relation to vitamin D status and disease activity in adults with systemic lupus erythematosus

Lupus ◽

10.1177/0961203318815593 ◽

2018 ◽

Vol 28 (2) ◽

pp. 156-162 ◽

Cited By ~ 5

Author(s):

A Sarkissian ◽

V Sivaraman ◽

S Bout-Tabaku ◽

S P Ardoin ◽

M Moore-Clingenpeel ◽

...

Keyword(s):

Systemic Lupus Erythematosus ◽

Vitamin D ◽

Bone Resorption ◽

Bone Turnover ◽

Disease Activity ◽

Lupus Erythematosus ◽

Bone Turnover Markers ◽

Systemic Lupus ◽

25 Hydroxy Vitamin D ◽

Turnover Markers

Objective Patients with systemic lupus erythematosus (SLE) have altered bone metabolism and are at risk of osteoporosis. The aim of this study was to examine bone turnover markers in relation to vitamin D, disease activity, and clinical risk factors in patients with established SLE. Methods Clinical registry and biorepository data of 42 SLE patients were assessed. Serum samples were analyzed for osteocalcin as a marker of bone formation, C-terminal telopeptide of type 1 collagen (CTX) as a marker for bone resorption, and 25-hydroxy vitamin D. Results Patients with a Systemic Lupus Erythematosus Disease Activity Index 2000 (SLEDAI) score of 3 or greater had a lower median osteocalcin level ( P = 0.02) and lower 25-hydroxy vitamin D levels ( P = 0.03) than those with a score of less than 3. No significant differences in bone turnover markers were observed between patients dichotomized into subgroups using a 25-hydroxy vitamin D cut-off of 30 ng/mL or by a daily prednisone dose greater than or 5 mg or less. Osteocalcin levels were negatively correlated with SLEDAI scores ( P = 0.034), and were positively correlated with the CTX index (a ratio of measured CTX value to the upper limit of the normal value for age and gender) ( P < 0.01). No association between the CTX index and SLEDAI scores was found. Conclusion SLE disease activity may have direct effects on bone formation, but no effects on bone resorption in this cohort of established SLE patients, probably related to the inflammation-suppressing effects of glucocorticoids, thereby inhibiting cytokine-induced osteoclast activity. A fine balance exists between disease control and the use of glucocorticoids with regard to bone health.

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