scholarly journals Revealing contrasting genetic variation and study of genetic diversity in urdbean (Vigna mungo (L.) Hepper) using SDS-PAGE of seed storage proteins

2016 ◽  
Vol 7 ◽  
Author(s):  
Swapan Kumar Tripathy ◽  
Priyadarshini Mohanty ◽  
Monalisha Jena ◽  
Gokul Bihari Dash ◽  
Kedareswar Pradhan ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Storage Protein ◽  
Storage Proteins ◽  
Seed Storage Protein ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Similarity Index ◽  
Seed Storage ◽  
Protein Profiling ◽  
Sds Page

<p><strong>Total seed storage protein profiles of 20 urdbean genotypes including the popular variety T9 were analysed by Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). 14 genotypes could be clearly identified based on genotype-specific seed protein fingerprints while rest of the test genotypes were categorized into three protein types. Dendrogram based on electrophoretic data clustered the genotypes into seven groups at 78.5% phenon level.  TU 95-1 with TU 12-25-4 revealed lowest similarity index value (0.33) followed by TU 95-1 with PU 30 and KU 96-3(SI=0.35). Clustering pattern revealed distinctly divergent group formed by TPU 95-1 and TPU 4. These may serve as a valuable source genotype in recombination breeding.   </strong></p><p><strong>Key words: </strong>Seed storage protein profiling, SDS-PAGE, Genetic variation, urdbean.<strong></strong></p>

Revealing genetic diversity in land races and wild accessions of mungbean [Vigna radiata (L.) Wilczek] using SDS-PAGE of seed storage proteins

2015 ◽  
Author(s):  
Ananya Panda ◽  
Swapan K. Tripathy
Keyword(s):  
Storage Proteins ◽  
Seed Storage Protein ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Land Races ◽  
Sds Page ◽  
Wild Accessions ◽  
Total Seed

Total seed storage protein profiles of 74 mungbean land races, three wild accessions and a popular variety ‘Jyoti’ of Odisha were analysed by Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). 32 genotypes could be clearly identified based on genotype-specific seed protein fingerprints while rest of the test genotypes were categorized into eight protein types. Genotypes included in each protein type had 100% homology and some of these could be duplicates. In this pursuit, a few specific polypeptide markers have been detected for identification of the land races/ genotypes. Dendrogram based on electrophoretic data clustered the genotypes into seven groups at 70% phenon level. Paralakhemundi local, Samarjhola local and Phulbani local-D; and three wild accessions (TCR 20, TCR 213 and TCR 243) were comparatively divergent from other genotypes. Besides, Jyoti, Kalahandi local 2A, Sikri local, kodala local A and TCR 20 were identified to be protein rich with high seed yield. TCR 20 being morphologically similar to mungbean, moderately high protein content and high yielding as well as resistant to drought and bruchids; it may serve as a valuable source genotype in recombination breeding

Profiling the Seed Storage Protein Among Different Genotypes of Trigonella foenum-graecum L. (Fenugreek)

2019 ◽  
Author(s):  
Nisha . ◽  
Priyanka Khati ◽  
P B Rao
Keyword(s):  
Storage Protein ◽  
Gel Electrophoresis ◽  
Storage Proteins ◽  
Seed Storage Protein ◽  
Sodium Dodecyl ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Protein Band ◽  
Trigonella Foenum Graecum ◽  
Trigonella Foenum Graecum L

A qualitative as well as quantitative categorization of seed storage proteins profiles of 23 genotypes of Trigonella foenum- graecum L. were performed by using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) for exploring the level of genetic discrepancy at seed storage protein level. Total soluble proteins were resolved on 10% resolving gel. A dendrogram was constructed on the basis of weight of seed storage proteins, which divide total genotypes into two groups further classified into different sub groups containing different genotypes in them. The bands obtained from gel electrophoresis can serve as a potent tool in discrimination of different genotypes on the basis of their protein content. Proteins with molecular weight 66, 43 and 35 kDa were found in all the genotypes except Fgk-76, PR, Rmt-303, PEB and Rmt-361, The 43 kDa protein band was found missing in Fgk-67, AFg-2, AM-2, AFg-4, Fgk-73, although the protein with 35 kDa weight was present in all the genotypes but not in Rmt-303 same as 63 kDa which is not present in Fgk-70 and 55 kDa protein band was found missing in Fgk-67, Afg-4 and Rmt-361.

Revealing genetic variation in upland rice using seed storage protein profiling

2015 ◽  
Vol 16 (2) ◽  
pp. 320
Author(s):  
Swapan K. Tripathy ◽  
B. R. Mohapatra ◽  
P. K. Nayak ◽  
S. Pal ◽  
N. Senapati ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Storage Protein ◽  
Seed Storage Protein ◽  
Upland Rice ◽  
Seed Storage ◽  
Protein Profiling

scholarly journals Identification of barley cultivars using SDS-PAGE electrophoresis

1992 ◽  
Vol 1 (1) ◽  
pp. 73-82
Author(s):  
Janne Roininen ◽  
Eero Nissilä ◽  
Matti Puolimatka ◽  
Seppo Pulli
Keyword(s):  
Seed Storage Protein ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Seed Storage ◽  
Hordeum Vulgare L ◽  
Banding Patterns ◽  
Breeding Lines ◽  
Barley Cultivars ◽  
Sds Page ◽  
Single Seeds

Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was applied to cultivar identification. Three different extractions methods were used to extract and fractionate the seed storage protein subunits from crushed single seeds of barley (Hordeum vulgare L.). Fifty-four genotypes including breeding lines and released cultivars were analysed and grouped according to the variation found in their protein banding patterns. In the first extraction, eight genotypes showed unique hordein subunit composition whilst remainder fell into 11 groups of 2 to 8. The other two extractions were carried out to characterize those genotypes producing identical banding patterns when using the first method. Relative mobility (REM) values for hordein bands were determined. Genetic background was found to strongly effect the determination of hordein composition of barley genotypes. Those genotypes with largely common ancestry showed often similar hordein composition and were difficult to identify whereas genotypes possessing unique hordein banding patterns had clearly exceptional pedigree. The effect of the row-type on hordein banding pattern was not clear as both two-row and many-row barleys were found to produce identical patterns. Intra-cultivar hordein polymorfism was found in three cultivars.

Assessment of Genetic Variation in Selected Members of Family Apocynaceae using SDS-PAGE

2021 ◽  
Vol 27 (1) ◽  
Author(s):  
Ravindra Singh ◽  
Mahendra Pal Singh ◽  
Rajdeep Kudesia
Keyword(s):  
Genetic Variation ◽  
Plant Species ◽  
Catharanthus Roseus ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Similarity Index ◽  
Group Method ◽  
Rauvolfia Serpentina ◽  
Thevetia Peruviana ◽  
Sds Page

Genetic variation of any plant species is very interesting in reducing genetic vulnerability as well as stabilizing production. In this regard, a study was undertaken to analyze the genetic variation among selected members of family Apocynaceae by using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). In this study, a total of 53 protein bands were analyzed, out of them all bands were polymorphic with a total of 100% polymorphism. The electrophoresis of the proteins revealed protein bands in the range of 151 kD to less than 11 kD molecular weight. The similarity index calculated on the basis of presence and absence of protein bands ranged from 0.04 to 0.200. A dendrogram was constructed based on UPGMA (unweighted pair group method using arithmetic averages) clustering method revealed three clusters. Cluster I contained three species namely Thevetia peruviana, Catharanthus roseus and Nerium indicum, in which Thevetia peruviana and Catharanthus roseus were more close than Nerium indicum, while cluster II included only one species namely Rauvolfia serpentina. Carissa carandus emerged as the most primitive species forming an out group (cluster III). Thus, this study revealed that the SDS-PAGE method plays a key role in the study of protein based variation among selected plant species.

scholarly journals Evaluation of effective protein extraction procedure to profile petiole of Moringa oleifera

2020 ◽  
Vol 7 (2) ◽  
pp. 214
Author(s):  
Zetty Amirah Zulkifli ◽  
Zaidah Rahmat
Keyword(s):  
Moringa Oleifera ◽  
Protein Extraction ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Extraction Procedure ◽  
Electrophoretic Pattern ◽  
Antioxidant Property ◽  
Extraction Methods ◽  
Protein Profiling ◽  
Sds Page

Moringa oleifera is widely known as multipurpose tree since all of its parts confer multiple functions. The leaf is highly favourable among consumers while the petiole is mostly wasted. There are numerous studies on the flavonoid and antioxidant property of the stem and twig. However, study on the petiole has never been done. There-upon, this study was conducted to develop protein profiling of the petiole. In this study, 6 different protein extraction methods were tested on the fresh petiole before its protein quantity and quality were checked via Bradford assay and Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) respectively. The in-solution digestion was then done prior to LC-MS/MS analysis. The protein electrophoretic pattern from the SDS-PAGE proves that method 6 using Tris HCl buffer with incorporation of dithiothreitol (DTT) and phenylmethylsulfonyl fluoride (PMSF) confers the best quality of protein. It produced the highest number of visible individual bands compared to other methods. Meanwhile, 93 proteins were successfully identified via LCMS analysis where the protein, signal response and carbohydrate metabolism categories confer the highest percentage. High quality and content of the protein extracted from the petiole including the antioxidant, anticancer and antidiabetic protein identified suggested that consuming this part of the plant could enhance nutrients of human body.

scholarly journals SDS-Page Seed Storage Protein Profiles in Chili Peppers (Capsicum L.)

2010 ◽  
Vol 2 (3) ◽  
pp. 86-90 ◽  
Author(s):  
Owk ANIEL KUMAR ◽  
Sape SUBBA TATA
Keyword(s):  
Storage Protein ◽  
Seed Storage Protein ◽  
Seed Storage ◽  
Protein Profiles ◽  
Sds Page ◽  
Chili Peppers

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