scholarly journals Advanced microscopy applied to the study of photodynamic mechanism in bacteria

2019 ◽  
Author(s):  
Júlia Bertagna ◽  
Monica Alonso Co ◽  
Duber M. M. Munar ◽  
Edmyr R. Reis ◽  
Rickson C. Mesquita
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Death ◽  
Photodynamic Therapy ◽  
Protoporphyrin Ix ◽  
Wide Field ◽  
Photodynamic Effect ◽  
Force Microscopy ◽  
Atomic Force

Photodynamic therapy (PDT) is an emergent technique used in clinical treatments for several pathologies, in which the photoactivation of photosensitizer molecule leads to cell death. Our project investigates the photodynamic effect of Protoporphyrin IX (PpIX), one of the currently most used photosensitizers, in prokaryotic cells, in order to explore its use in sterilization. Atomic Force Microscopy (AFM) and Wide Field Microscopy (WFM) were used to evaluate PDT effects on X.fastidiosa cells.

Characterization of Antimicrobial Photodynamic Therapy-TreatedStreptococci mutans: An Atomic Force Microscopy Study

2013 ◽  
Vol 31 (3) ◽  
pp. 105-109 ◽  
Author(s):  
Mary Anne Sampaio de Melo ◽  
Juliana Paiva Marques Lima Rolim ◽  
Iriana Carla Junqueira Zanin ◽  
Eduardo Bede Barros ◽  
Erivelton Façanha da–Costa ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Photodynamic Therapy ◽  
Microscopy Study ◽  
Antimicrobial Photodynamic Therapy ◽  
Atomic Force Microscopy Study ◽  
Force Microscopy ◽  
Atomic Force

The Synthesis of Methylene Blue Photosensitiser Conjugated with Gold Nanoparticles

2013 ◽  
Vol 829 ◽  
pp. 299-303 ◽  
Author(s):  
Hasan Kariminezhad ◽  
Sona Khayat Omrani ◽  
Setareh Habibzadeh ◽  
Maria Agheb
Keyword(s):  
Methylene Blue ◽  
Gold Nanoparticles ◽  
Atomic Force Microscopy ◽  
Fourier Transform ◽  
Photodynamic Therapy ◽  
Infrared Spectroscopy ◽  
Cellular Uptake ◽  
Force Microscopy ◽  
Atomic Force ◽  
Uv Visible

Nowadays, nanocarriers possess growing interest for medical usage such as photodynamic therapy. Conjunction of these nanoparticles with photosensitising drugs leads to more cellular uptake, higher photocytotoxicty, higher biocompatibility and more selectivity. Previous papers conclude that the 50 nm size nanoparticles give the highest cellular uptake for the purpose of photodynamic therapy. In this paper the gold nanoparticles were synthesized based on TurkevichFrens method. Moreover, methylene blue as a relevant photosensitiser was conjugated to achieved AuNPs. The spectrometric results of UV-visible and Fourier Transform Infrared spectroscopy (FTIR) as well as Atomic Force Microscopy (AFM) emphasis on the presence of MB sensitizers connected to resulting 50 nm gold nanoparticles.

Identification and ultrastructural imaging of photodynamic therapy-induced microfilaments by atomic force microscopy

2009 ◽  
Vol 109 (12) ◽  
pp. 1428-1434 ◽  
Author(s):  
Se-Hui Jung ◽  
Jin-Young Park ◽  
Je-Ok Yoo ◽  
Incheol Shin ◽  
Young-Myeong Kim ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Photodynamic Therapy ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Photodynamic Therapy With Propolis: Antibacterial Effects on Staphylococcus aureus, Streptococcus mutans and Escherichia coli Analysed by Atomic Force Microscopy

2020 ◽  
Vol 11 (Suppl 1) ◽  
pp. S107-S112
Author(s):  
Alice de Morais Veras Fonseca ◽  
Guilherme Herbet Leite Sampaio ◽  
Wládia Pontes Araujo ◽  
Raí Emanuel Silva ◽  
Fábio de Oliveira Silva Ribeiro ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Atomic Force Microscopy ◽  
Photodynamic Therapy ◽  
Streptococcus Mutans ◽  
Antibacterial Effects ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Nanomechanics in Monitoring the Effectiveness of Drugs Targeting the Cancer Cell Cytoskeleton

2020 ◽  
Vol 21 (22) ◽  
pp. 8786
Author(s):  
Andrzej Kubiak ◽  
Tomasz Zieliński ◽  
Joanna Pabijan ◽  
Małgorzata Lekka
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Death ◽  
Actin Filaments ◽  
High Spatial Resolution ◽  
Living Cells ◽  
Antitumor Drugs ◽  
Antitumor Therapy ◽  
Force Microscopy ◽  
Nanomechanical Properties ◽  
Atomic Force

Increasing attention is devoted to the use of nanomechanics as a marker of various pathologies. Atomic force microscopy (AFM) is one of the techniques that could be applied to quantify the nanomechanical properties of living cells with a high spatial resolution. Thus, AFM offers the possibility to trace changes in the reorganization of the cytoskeleton in living cells. Impairments in the structure, organization, and functioning of two main cytoskeletal components, namely, actin filaments and microtubules, cause severe effects, leading to cell death. That is why these cytoskeletal components are targets for antitumor therapy. This review intends to describe the gathered knowledge on the capability of AFM to trace the alterations in the nanomechanical properties of living cells induced by the action of antitumor drugs that could translate into their effectiveness.

Sign in / Sign up

Export Citation Format

Share Document