scholarly journals Investigation of human plasma depletome from patients with schizophrenia

2019 ◽  
Author(s):  
Licia Carla da Silva Costa ◽  
Daniel Martins de Souza ◽  
Sheila Garcia Rosa ◽  
Paulo A. Baldasso ◽  
Johann Steiner
Keyword(s):  
Mass Spectrometry ◽  
Blood Plasma ◽  
Psychiatric Disorders ◽  
Treatment Outcomes ◽  
Human Plasma ◽  
High Abundance ◽  
Improve Treatment ◽  
Dynamic Concentration ◽  
Shotgun Mass Spectrometry ◽  
Potential Biomarkers

The proteome of blood plasma is an interesting source of biomarkers and a potential way to improve treatment outcomes in psychiatric disorders. Respire that, its wide dynamic concentration range makes reducing its complexity necessary. Thus, in proteomic studies, a few of the most abundant proteins are depleted and normally discarded. This high-abundance fraction, called the depletome, however, is a source of potential biomarkers due to nonspecific bindings with low abundance proteins. In this work, we aimed to characterize the high-abundance fraction using a shotgun mass spectrometry approach. These proteins show the importance of studying depletome proteins in the quest for biomarkers.

scholarly journals The possibility of using PlasmaDeepDive™ MRM panel in clinical diagnostics

2015 ◽  
Vol 61 (2) ◽  
pp. 272-278
Author(s):  
Iu.V. Miroshnichenko ◽  
N.A. Petushkova ◽  
N.E. Moskaleva ◽  
N.B. Teryaeva ◽  
V.G. Zgoda ◽  
...  
Keyword(s):  
Nervous System ◽  
Blood Plasma ◽  
Human Plasma ◽  
Human Blood ◽  
Proteomic Analysis ◽  
Clinical Diagnostics ◽  
Human Blood Plasma ◽  
Plasma Samples ◽  
Human Plasma Samples ◽  
Potential Biomarkers

Concentrations of 46 proteins have been determined in human blood plasma using PlasmaDeepDive™ MRM Panel ("Biognosys AG", Switzerland). 18 of them were included into the group of proteins with higher concentrations, also identified by the shotgun proteomic analysis. Based on literature data it is concluded that the PlasmaDeepDive™ MRM Panel is applicable for studies of human plasma samples for potential biomarkers of various nervous system disorders.

Diagnosis of pancreatic cancer via1H NMR metabolomics of human plasma

The Analyst ◽  
2018 ◽  
Vol 143 (24) ◽  
pp. 5974-5978 ◽  
Author(s):  
Lenka Michálková ◽  
Štěpán Horník ◽  
Jan Sýkora ◽  
Lucie Habartová ◽  
Vladimír Setnička
Keyword(s):  
Pancreatic Cancer ◽  
Blood Plasma ◽  
Human Plasma ◽  
Nmr Metabolomics ◽  
H Nmr ◽  
Potential Biomarkers

The investigation of blood plasma via1H NMR metabolomics revealed a panel of potential biomarkers of pancreatic cancer.

scholarly journals Analysis of α- and β-amanitin in Human Plasma at Subnanogram per Milliliter Levels by Reversed Phase Ultra-High Performance Liquid Chromatography Coupled to Orbitrap Mass Spectrometry

Toxins ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 671
Author(s):  
Thomas P. Bambauer ◽  
Lea Wagmann ◽  
Armin A. Weber ◽  
Markus R. Meyer
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Blood Plasma ◽  
Human Plasma ◽  
Human Blood ◽  
Reversed Phase ◽  
Urine Samples ◽  
Human Blood Plasma ◽  
Plasma Samples ◽  
Valuable Alternative

Amatoxins are known to be one of the main causes of serious to fatal mushroom intoxication. Thorough treatment, analytical confirmation, or exclusion of amatoxin intake is crucial in the case of any suspected mushroom poisoning. Urine is often the preferred matrix due to its higher concentrations compared to other body fluids. If urine is not available, analysis of human blood plasma is a valuable alternative for assessing the severity of intoxications. The aim of this study was to develop and validate a liquid chromatography (LC)-high resolution tandem mass spectrometry (HRMS/MS) method for confirmation and quantitation of α- and β-amanitin in human plasma at subnanogram per milliliter levels. Plasma samples of humans after suspected intake of amatoxin-containing mushrooms should be analyzed and amounts of toxins compared with already published data as well as with matched urine samples. Sample preparation consisted of protein precipitation, aqueous liquid-liquid extraction, and solid-phase extraction. Full chromatographical separation of analytes was achieved using reversed-phase chromatography. Orbitrap-based MS allowed for sufficiently sensitive identification and quantification. Validation was successfully carried out, including analytical selectivity, carry-over, matrix effects, accuracy, precision, and dilution integrity. Limits of identification were 20 pg/mL and calibration ranged from 20 pg/mL to 2000 pg/mL. The method was applied to analyze nine human plasma samples that were submitted along with urine samples tested positive for amatoxins. α-Amanitin could be identified in each plasma sample at a range from 37–2890 pg/mL, and β-amanitin was found in seven plasma samples ranging from <20–7520 pg/mL. A LC-HRMS/MS method for the quantitation of amatoxins in human blood plasma at subnanogram per milliliter levels was developed, validated, and used for the analysis of plasma samples. The method provides a valuable alternative to urine analysis, allowing thorough patient treatment but also further study the toxicokinetics of amatoxins.

scholarly journals Determination of the concentration range for 267 proteins from 21 lots of commercial human plasma using highly multiplexed multiple reaction monitoring mass spectrometry

The Analyst ◽  
2020 ◽  
Vol 145 (10) ◽  
pp. 3634-3644
Author(s):  
Claudia Gaither ◽  
Robert Popp ◽  
Yassene Mohammed ◽  
Christoph H. Borchers
Keyword(s):  
Mass Spectrometry ◽  
Human Plasma ◽  
Multiple Reaction Monitoring ◽  
Reaction Monitoring ◽  
Biomarker Validation ◽  
Potential Biomarkers

Multiple reaction monitoring (MRM) is a key tool for biomarker validation and the translation of potential biomarkers into the clinic.

Remarkable temporal stability of high-abundance human plasma proteins assessed by targeted mass spectrometry

2012 ◽  
Vol 6 (11-12) ◽  
pp. 626-634 ◽  
Author(s):  
Sarah A. Randall ◽  
Matthew J. McKay ◽  
Dana Pascovici ◽  
Kate Mahon ◽  
Lisa Horvath ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Human Plasma ◽  
Plasma Proteins ◽  
Temporal Stability ◽  
High Abundance

Validation of Simvastatin Analysis Methods in Human Blood Plasma (In Vitro) Using HPLC-UV Detector

2018 ◽  
Vol 8 (2) ◽  
Author(s):  
Iyan Sopyan ◽  
Cynthia Jaya ◽  
Driyanti Rahayu
Keyword(s):  
Blood Plasma ◽  
Human Plasma ◽  
Disease Risk ◽  
Protein Precipitation ◽  
Precipitation Method ◽  
Human Blood Plasma ◽  
Analysis Method ◽  
Uv Detector ◽  
Number Of Patients ◽  
System Suitability

The use of simvastatin (SV) increases along with the increasing number of patients with hyperlipidemia and cardiovascular disease risk factors. Consequently, this condition leads to the increasing need of analytical determination of SV in blood plasma. Analysis of SV in human plasma using protein precipitation method and HPLC with UV detector has not been reported. This research was purpose to find out the rapid, accurate, and valid of SV analysis method in human plasma. In this research plasma samples were treated with protein precipitation method. The analyte was then analyzed using HPLC with C18 column 250x4 mm and 5 µm of particle size, the mobile phase contained of phosphate buffer 0.01 M (pH 4.0) and acetonitrile 30:70 v/v with flow rate 1 mL/minute, and detected at 239 nm. The analysis method was validated based on some parameters, such as selectivity, accuracy, precision, repeatability, linearity, LOD, LOQ, and system suitability. The result showed selectivity represented by Rs was 2.870, repeatability by its CV less than 2%, and linearity by its coefficient correlation (r) 0.9992 for concentration range 0.08-0.32 ppm. Based on chromatogram peak area, LOD and LOQ were 0.0132 and 0.0440 ppm respectively, accuracy and precision were 86.25-89.36% and 0.66-1.81% were obtained. The result of system suitability test from retention time and chromatogram peak area showed by its CV less than 2%. The analysis method was proved to be valid for SV analysis in human plasma

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