Development of a novel microbiological method for detection of gamma irradiated spices

Irina Mladenovska; Nora Limani Bektashi; Beti Andonovic; Hristina Spasevska; Ivana Sandeva; Milica Arizanova; Slobodan Masic; Arianit A. Reka

doi:10.20450/mjcce.2021.2397

Screening of gamma irradiated spices in Korea by using a microbiological method (DEFT/APC)

Food Control ◽

10.1016/s0956-7135(02)00108-1 ◽

2003 ◽

Vol 14 (7) ◽

pp. 489-494 ◽

Cited By ~ 15

Author(s):

K.N. Oh ◽

S.Y. Lee ◽

H.J. Lee ◽

K.E. Kim ◽

J.S. Yang

Keyword(s):

Microbiological Method ◽

Gamma Irradiated

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Enterococcal Virulence Determinants In Urinary Tract Infection Patients

Bangladesh Journal of Medical Microbiology ◽

10.3329/bjmm.v6i1.19361 ◽

2012 ◽

Vol 6 (1) ◽

pp. 14-17

Author(s):

Jabin Akhter ◽

Shaheda Anwar ◽

Sharmeen Ahmed

Keyword(s):

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Virulence Factors ◽

Virulence Determinants ◽

Microtitre Plate ◽

Microbiological Method ◽

Enterococcal Infection ◽

Microtitre Plate Assay ◽

Positive Frequency

Urinary tract infection caused by Enterococci has become frequent occurrences in health care settings. Currently they emerged with increasing resistance to multiple antibiotics. Haemolysin, gelatinase and biofilm production are some markers that have been proposed as possible Enterococcal virulence factors. In view of the increasing importance of Enterococcal infection, the present study was designed to isolate and identify the Enterococci to the species level from urine of urinary tract infection patients and to investigate their possible virulence factors. Biofilm was detected on polystyrene microtitre plate to see the adherence of microorganism. Haemolysin production and gelatin hydrolysis detected by standard microbiological method. Fifty nine enterococcal isolates were speciated by conventional microbiological method and examined for their ability to form biofilm by microtitre plate assay. In this study, biofilm formations by Enterococci were found in 83.33% isolates from catheterized and 56.09% from non-catheterized patients. Aong them, E.faecalis & 50% E.faecium produced biofilm. About 43.63% E.faecalis & 10% E.faecium produced haemolysin and only one isolate were found to be gelatinase positive. Frequency of virulence factors (VFs) in combination was observed in this study. Two VFs (haemolysin and biofilm) were observed in 27.11% in combination and 3 VFs ( haemolysinm biofilm and gelatinase) were present in 1.69% isolates. These results suggest that although there may not be an absolute role for individual virulence determinants in infectivity, combinations of factors may play a role in allowing a biofilm infection to be more resistant to therapy.DOI: http://dx.doi.org/10.3329/bjmm.v6i1.19361 Bangladesh J Med Microbiol 2012; 06(01): 14-17

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Effect of Carbon Coating on the Properties of Gamma Irradiated Ultra-High-Molecular-Weight Polyethylene Specimens

Critical Reviews in Biomedical Engineering ◽

10.1615/critrevbiomedeng.v28.i34.160 ◽

2000 ◽

Vol 28 (3-4) ◽

pp. 445-449 ◽

Cited By ~ 1

Author(s):

Debi P. Mukherjee ◽

Alan L. Ogden ◽

R. H. Mayeux ◽

U. Siriwardane ◽

H. R. Patel

Keyword(s):

Molecular Weight ◽

High Molecular Weight ◽

Carbon Coating ◽

Gamma Irradiated ◽

Ultra High Molecular Weight

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Presentation of Gamma-Irradiated-in-Air Polyethylene Wear in the Form of a Synovial Cyst

Journal of Automation and Information Sciences ◽

10.1615/jlongtermeffmedimplants.v13.i5.60 ◽

2003 ◽

Vol 13 (5) ◽

pp. 413-418

Author(s):

Bryan T. Edwards ◽

Patrick B. Leach ◽

R. Scott Corpe ◽

Robert D. Zura ◽

Timothy R. Young

Keyword(s):

Polyethylene Wear ◽

Synovial Cyst ◽

Gamma Irradiated

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Microbiological Method for Eor

Revue de l Institut Français du Pétrole ◽

10.2516/ogst:1990010 ◽

1990 ◽

Vol 45 (1) ◽

pp. 115-121 ◽

Cited By ~ 4

Author(s):

E. M. Yulbarisov

Keyword(s):

Microbiological Method

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Employing Gamma Ray Irradiated Giardia lamblia as Trialed Vaccine in Experimental Animal

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.9.2.19 ◽

2019 ◽

Vol 9 (02) ◽

Author(s):

Amal Kamil Abdul Sada ◽

Amany Mohamed Al-Kaysi

Keyword(s):

Giardia Lamblia ◽

Gamma Ray ◽

Age Groups ◽

Active Phase ◽

Study Groups ◽

Negative Control ◽

Control Group ◽

High Dose ◽

Histopathological Changes ◽

Gamma Irradiated

This is an experimental trial to prepare a vaccine from gamma-irradiated Giardia lamblia which is evaluated in experimental animals. The study was conducted from December 2015 to April 2016. The field survey of the parasite was conducted from those patients attending the laboratories of the Alawi Children's Hospital in Rusafa and the Al-Yarmouk Teaching Hospital in Karkh, through which 1250 stool samples of different age groups were examined. Five groups of mice were used in the study; the first was injected with normal saline and considered as a negative control group, the second was injected with cystic form of non-irradiated Giardia lamblia and considered as a positive control group, whereas the other three groups were injected with gamma irradiated Giardia lamblia at three different doses 10, 15 and 25 rad respectively. Giardia lamblia was primarily cultivated in liver infusion agar for ten days to obtain the active phase. On the sixth day, the cystic phase was purified and standardized to be used in the infection of mice with or without the exposure of gamma rays. Mice showed high sensitivity to parasitic infestation, in the gamma non-irradiated and the irradiated with gamma 10 rad, and 15 rad irradiated groups which was 100%. The results expressed an excystation process of the depleted phases and the release of the feeder phases. The results of the three irradiated groups consisted of histopathological changes of the small, and the rectum by dissection after two weeks of infection, with intestine amputation lesions, as well as ulceration and inflammation of the inflammatory cells represented in small numbers of neutrophil, lymphocytes, and eosinophils. The presence of ulceration and fall of epithelial cells in the intestinal cavity has been shown, and different forms of the parasite have been observed. Mice which was injected with irradiated G lamblia at high dose (25 rad), not show and sensitivity to the challenge infection and no excystation of thy parasite had been done. After 2 wreaks, a comparison was achieved between all study groups in which no histopathological changes were noticed in the mice irradiated with dose of25 rad. After another two weeks, a challenge dose was given (un-attenuated G lamblia) and mice were dissected after another two weeks, no changes on the level of histopathology of intestinal tissue were noticed the results suggested that mice acquire an immunity against the parasite infection.

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