scholarly journals EFFECT OF ROOM TEMPERATURE ON THE QUALITY OF DNA FROM EARPHONE SWAB BY OBSERVING MITOCHONDRIAL DNA [mtDNA] D-LOOP REGION OF 126 bp (HVS II, nt 34-159) AND 143 bp (HVS I, nt 16268-16410)

2017 ◽  
Vol 53 (2) ◽  
pp. 86
Author(s):  
Ahmad Yudianto ◽  
Nola Margaret
Keyword(s):  
Room Temperature ◽  
Dna Analysis ◽  
Prolonged Exposure ◽  
Forensic Identification ◽  
Loop Region ◽  
Buccal Swabs ◽  
Vaginal Swabs ◽  
Long Time ◽  
D Loop

The objects contained in crime scene and its surroundings have significance in the examination of forensic identification. The most commonly used specimens in the examination for identification are blood/blood spots, semen patches, vaginal swabs, buccal swabs and bones, including items often used by the perpetrator/victim the last time. For example, a mobile phone hearing aid (headset/earphone). In the use of earphones attached to the outer ear skin so it is suspected there is a serumen attached to the tool. One factor that can affect the quality of DNA is the prolonged exposure. Until now in Indonesia the effect of long exposure to room temperature on the quality of DNA on the DNA material of earphone swabs through DNA analysis has not been widely known. The type of study was laboratory experimental. Used earphones are exposed at room temperature within 1, 7, 14 and 20 days. The results of this study indicate that the environmental effect, ie the duration of exposure, affects the decrease in DNA content significantly from day 1 to 20. Detection of PCR mtDNA D-Loop HVS I visualization results 143 bp nt: 16268 16410 shows positive detection results (+) Only at day 1 exposure to room temperature [4 sample/66,67%] and day 7 [3 sample/50%]. Visualization of PCR results mtDNA D-Loop HVS II 126bp nt: 34 -159 was performed on day 1 day room exposure [2 sample/33,37%] and day 7 [6 sample/100%]. Conclusion, the duration of exposure to room temperature affect the quality of DNA from earpiece swab material. Decreased levels of earphones DNA swabs showed a significance value (p <0.005) against the effect of long time exposure to room temperature.

scholarly journals ANALYSIS OF EARPHONE SWAB MITOCHONDRIAL DNA AS AN ALTERNATIVE MATERIAL FOR IDENTIFICATION EXAMINATION

2017 ◽  
Vol 52 (3) ◽  
pp. 169
Author(s):  
Ahmad Yudianto ◽  
Yeti Eka Sispitasri ◽  
Nola Margaret
Keyword(s):  
Mitochondrial Dna ◽  
Hearing Aids ◽  
Room Temperature ◽  
Nuclear Dna ◽  
Personal Identification ◽  
Identification Methods ◽  
Dna Assay ◽  
Buccal Swabs ◽  
Vaginal Swabs ◽  
Alternative Material

Identification include fingerprint, property, medical, dental, serologic and exclusion methods. In the development, identification methods led to molecular forensics, a new field of science evolving since the 1980s, known as DNA fingerprinting. Specimens widely used in DNA assay for identification are blood spots/bloods, semen spots, vaginal swabs, buccal swabs and bones. In addition to these specimens, the last objects often used by the perpetrators/victims can be used, such as hearing aids (headsets/earphones). In its use, earphones are attached to the outer ear skin; thus, the earwax is suspected to adhere to the device. To date, in Indonesia personal identification is performed through swabs of earphones/headsets using the DNA profiling method. In particular, mitochondrial DNA has not been widely used for identification. The present study was of laboratory experimental. Earphones which have been used for 3 days were placed in room temperature for 1, 7, 14 and 20 days. Results showed that the environmental factor of exposure duration had an effect of a significant decrease in the levels of DNA from day 1 to day 20. Only 126-bp mtDNA (HVS II) was detected on the samples of day 1 and continued with sequencing. Mitochondrial DNA has better durability and relatively higher number of copies than those of nuclear DNA. This leads to greater possibility of success in amplification, given the higher number of mitochondrial DNA copies and the fact that mitochondrial DNA is a single locus that allows recombination.

scholarly journals Effect of Storage Condition on Seed Quality of Some Dhaincha (Sesbania spp.) Accessions

2016 ◽  
Vol 14 (1) ◽  
pp. 83-91
Author(s):  
M Towhidul Karim ◽  
Fazlul Haque ◽  
A K M Golam Sarwar ◽  
M Ayub Ali
Keyword(s):  
Room Temperature ◽  
Seed Quality ◽  
Storage Condition ◽  
Storage Conditions ◽  
Pathology Laboratory ◽  
Seed Pathology ◽  
Vigour Index ◽  
Long Time ◽  
Fusarium Sp

An experiment was conducted at the Seed Pathology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, to understand the effect of storage conditions on seed quality of different accessions of dhaincha (Sesbania spp.). Seeds of twenty accessions were collected and stored in a refrigerator (4°C) and laboratory at normal room temperature (25-30°C). Sprouting (%), germination (%), vigour index and the incidence of seed-borne pathogens were recorded following ISTA procedures. Germination and emergence were higher when seeds were stored in the refrigerator than those in room temperature. The vigour index was also high when the seeds were stored in a refrigerator. Fungi belonging to genera Aspergillus, Fusarium, Rhizopus, and Penicillium were associated with seeds of the different accessions of dhaincha. Seed borne infection of Fusarium sp. was highest (14%) in G-13 when seeds were stored at room temperature. It was evident from the results that the quality of the seeds of dhaincha accessions was retained for a long time when they were stored at low (around 4°C) temperature.The Agriculturists 2016; 14(1) 83-91

scholarly journals Impact of storage on sensory quality of blackcurrant juices prepared with or without enzymatic treatment at industrial scale

2020 ◽  
Vol 246 (12) ◽  
pp. 2611-2620
Author(s):  
Oskar Laaksonen ◽  
Leenamaija Mäkilä ◽  
Mika Jokinen ◽  
Tapio Metz ◽  
Heikki Kallio ◽  
...  
Keyword(s):  
Room Temperature ◽  
Sensory Quality ◽  
Enzyme Treatment ◽  
Industrial Scale ◽  
Colour Intensity ◽  
Long Time ◽  
The Stability ◽  
Sensory Profiles ◽  
Monomeric Anthocyanins

AbstractBlackcurrant juices prepared with or without enzyme treatment at industrial scale were stored at room temperature or + 4 °C for 1 year, and their sensory profiles, chemical composition and rheological properties were investigated. The juice without enzyme treatment had less intense colour and it was perceived and measured as more viscose than the juice produced with enzyme assistance. Content of monomeric anthocyanins decreased significantly during storage in both juice types under both storing conditions. However, changes in colour intensity were observed only in the non-enzymatic juices. In the discrimination tests based on the appearance, the stored samples were detected as significantly different. Storage resulted in significant changes in contents of hydroxycinnamic acids and flavonols, but this did not have an impact on the bitterness or astringency of the juices. Moreover, sugar and acid contents were mainly unchanged during storage. Storage resulted in a decrease in dynamic viscosity and greater differences in viscoelastic properties for the juice with enzymatic assistance. This study provides important information about the stability of juices typically stored at room temperature for a long time.

scholarly journals Environmental Odour Nuisance Assessment in Urbanized Area: Analysis and Comparison of Different and Integrated Approaches

Atmosphere ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 690
Author(s):  
Tiziano Zarra ◽  
Vincenzo Belgiorno ◽  
Vincenzo Naddeo
Keyword(s):  
Prolonged Exposure ◽  
Industrial Plant ◽  
Dispersion Modelling ◽  
Time Analysis ◽  
Location Factors ◽  
Long Time ◽  
Analysis Programme ◽  
Odour Emissions ◽  
Short Time

Prolonged exposure to odour emissions causes annoyance which leads to nuisance and consequently to complaints. Different methodologies exist in the literature to evaluate odour impacts, but not all are suitable to assess environmental odour nuisance. Information about their applicability criteria and comparison, is scarce and referred to short time analysis. The research presents and discusses the application of different methods to characterize and assess odour nuisance around an industrial plant localized in a sensitive area. Experimental activities are carried out through a long-time analysis programme. Field inspections and predictive methods are investigated and compared. A modification of the traditional dispersion modelling approach is proposed in order to adapt its application for the prediction of the odour nuisance. The offensiveness and location factors are identified as key parameters in the quantification of the perceived nuisance. The integrated dispersion modelling multi-level approach is highlighted as the most suitable for defining the plant strategies. The paper provides useful information to characterize environmental odour problems and identify appropriate solutions for an effective management of odorous sources, with the aim of reducing complaints, restoring the proper relationship between odorous plants and the surrounding communities and increasing the overall quality of the environment.

scholarly journals Pengaruh Suhu Penyimpanan Obat Kapsul Komersil Terhadap Organoleptik

2020 ◽  
Vol 2 (1) ◽  
pp. 10-17
Author(s):  
Venny Diah Ningsih
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Room Temperature ◽  
Storage Temperature ◽  
Drug Product ◽  
Ascorbic Acid Content ◽  
Cold Temperature ◽  
Excessive Heat ◽  
Long Time

Medicinal products have quality standards or qualities that are very important to determine the therapeutic effect of a drug. One of the external factors that affect drug quality is temperature, because temperature can affect the instability of the quality of a drug product. This study aims to determine the effect of storage temperature of commercial capsule drugs on organoleptic. The research method uses a quantitative approach and experimental method by measuring the ascorbic acid content of the vitamin C tablet drug samples, with variations in temperature, namely cold (5°C), room temperature (27°C) and excessive heat (48°C) for a long time 180 minutes time. The results showed that temperature had a significant effect on the content of ascorbic acid in commercial vitamin C tablets with the results in sample I cold temperature (5°C) 101.2%, room temperature (27°C) 97.6% and overheating temperature (48°C). 90.5%. In sample II the cold temperature (5°C) was 101.8%, the room temperature (27°C) was 103.3% and the overheat temperature (48°C) was 95.3% after 180 minutes of storage.   Keywords: Temperature, Capsule Medicine, Organoleptic ABSTRAK   Mutu atau kualitas suatu produk obat merupakan faktor penting untuk mengetahui efek terapetik dari suatu obat. Salah satu faktor eksternal yang dapat mempengaruhi mutu obat adalah suhu karena dapat mempengaruhi ketidakstabilan sifat fisikakimia senyawa aktif dalam sediaan. Penelitian ini bertujuan untuk mengetahui pengaruh suhu penyimpanan obat kapsul komersil terhadap organoleptik. Metode penelitian menggunakan pendekatan kuantitatif dan eksperimental dengan cara mengukur kadar asam askorbat dari sampel obat tablet vitamin C dengan variasi suhu yaitu dingin (5°C), suhu ruang (27°C) dan suhu panas berlebih (48°C) dengan lama waktu simpan 180 menit. Hasil penelitian menunjukkan bahwa suhu berpengaruh secara nyata terhadap kandungan asam askorbat dalam obat tablet vitamin C komersil dengan hasil pada sampel I suhu dingin (5°C) 101.2%, suhu ruang (27°C) 97.6% dan suhu panas berlebih (48°C) 90.5%. Pada sampel II suhu dingin (5°C) 101.8%, suhu ruang (27°C) 103.3% dan suhu panas berlebih (48°C) 95.3% setelah 180 menit penyimpanan.   Kata Kunci: Suhu, Obat Kapsul, Organoleptik

scholarly journals Mitochondrial DNA Analysis for the Cytochrome b Gene and D-loop Region from the Bull Shark Carcharhinus leucas

1996 ◽  
Vol 62 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Toru Kitamura ◽  
Akira Takemura ◽  
Shugo Watabe ◽  
Toru Taniuchi ◽  
Makoto Shimizu
Keyword(s):  
Mitochondrial Dna ◽  
Dna Analysis ◽  
Loop Region ◽  
Carcharhinus Leucas ◽  
Mitochondrial Dna Analysis ◽  
D Loop ◽  
Bull Shark ◽  
I Gene

scholarly journals ANALISIS DNA MITOKONDRIA SWAB EARPHONE SEBAGAI BAHAN ALTERNATIF PEMERIKSAAN IDENTIFIKASI

1970 ◽  
Vol 7 ◽  
Author(s):  
Ahmad Yudianto ◽  
Yeti Eka Sispitasri ◽  
Nola Margaret
Keyword(s):  
Mitochondrial Dna ◽  
Hearing Aids ◽  
Exposure Duration ◽  
Room Temperature ◽  
Nuclear Dna ◽  
Personal Identification ◽  
Identification Methods ◽  
Dna Assay ◽  
Buccal Swabs ◽  
Vaginal Swabs

Identification include fingerprint, property, medical, dental, serologic and exclusion methods. In the development, identification methods led to molecular forensics, a new field of science evolving since the 1980s, known as DNA fingerprinting. Blood spots/bloods, semen spots, vaginal swabs, buccal swabs and bones are specimens widely used in DNA assay for identification. In addition to these specimens, the last objects often used by the perpetrators/victims can be used, such as hearing aids (headsets/earphones). In its use, earphones are attached to the outer ear skin; thus, the earwax is suspected to adhere to the device. To date, in Indonesia personal identification is performed through swabs of earphones/headsets using the DNA profiling method. In particular, mitochondrial DNA has not been widely used for identification. The present study was of laboratory experimental. Earphones which have been used for 3 days were placed in room temperature for 1, 7, 14 and 20 days. Results showed that the environmental factor of exposure duration had an effect of a significant decrease in the levels of DNA from day 1 to day 20. Only 126-bp mtDNA (HVS II) was detected on the samples of day 1 and continued with sequencing. Mitochondrial DNA has better durability and relatively higher number of copies than those of nuclear DNA. This leads to greater possibility of success in amplification, given the higher number of mitochondrial DNA copies and the fact that mitochondrial DNA is a single locus that allows recombination.  

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