scholarly journals The effect of canceroembryonic antigen on cytokine production by immunocompetent blood cells in patients with breast cancer

2018 ◽  
Vol 17 (3) ◽  
pp. 5-12 ◽  
Author(s):  
A. I. Autenshlus ◽  
T. A. Kunts ◽  
K. V. Karpukhina ◽  
E. S. Mikhailova ◽  
N. A. Varaksin ◽  
...  
Keyword(s):  
Invasive Ductal Carcinoma ◽  
Immune Cells ◽  
Blood Cells ◽  
Cytokine Production ◽  
Ductal Carcinoma ◽  
Precancerous Lesions ◽  
Breast Tumors ◽  
Precancerous Conditions ◽  
Immunocompetent Cells ◽  
Gm Csf

Aim. Investigation of the influence of canceroembryonic antigen (CEA) on the immune cells in patients with breast tumors.Materials and methods. Peripheral blood of 67 patients with invasive ductal carcinoma (n = 44), fibroadenoma (n = 12) and breast pre-cancer (n = 11). In the supernatants of the blood cells cytokine production induced by CEA was determined by ELISA.Results.It was found that CEA stimulated production of IL-6, IL-8, IL-10, IL-1β, IL-1Ra, TNFα, G-CSF, GM-CSF and the protein MCP-1 by blood immune cells in patients with fibroadenoma compared with groups of patients with invasive ductal carcinoma and precancerous lesions. Stimulation index of CEA on cytokine production by immunocompetent cells in patients in these groups was lower than in groups of patients with fibroadenoma due to initially high levels of spontaneous cytokine production.Results.  In  patients  with  fibroadenoma  CEA  was  found  to  stimulate  the  production  of  IL-6,  IL-8,  IL-10,  IL-1β,  IL-1Ra,  TNFa,  G-CSF,  GM-CSF  and  MCP-1  compared  with  groups  of  patients  with  invasive ductal carcinoma and precancerous conditions. Indexes of the influence of CEA on cytokine production by immunocompetent cells in these groups was lower than in the fibroadenoma due to initially high spontaneous production of cytokines.Conclusion.  Evident  spontaneous  cytokine-producing  function  of  immunocompetent  blood  cells  was revealed in patients with invasive ductal carcinoma and precancerous conditions compared to patients with fibroadenoma.  The  differences  between  the  indices  of  CEA  influence  on  cytokine  production  in  patients with malignant, benign and precancerous conditions can serve as a basis for the development of methods of differential diagnosis of breast tumors.

Cytokine production by blood immune cells, tumor and its microenvironment, characteristic of extracellular matrix in patients with invasive ductal carcinoma of no special type

2019 ◽  
Vol 65 (5) ◽  
pp. 424-431
Author(s):  
A.I. Autenshlyus ◽  
K.I. Davletova ◽  
A.A. Studenikina ◽  
E.S. Mikhaylova ◽  
N.A. Varaksin ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Invasive Ductal Carcinoma ◽  
Immune Cells ◽  
Cytokine Production ◽  
Ductal Carcinoma ◽  
Solid Phase ◽  
Enzyme Linked Immunosorbent Assay ◽  
Gm Csf ◽  
Lymphatic Metastases ◽  
The Moment

The aim of this research was to study cytokine production by blood immune cells, tumor, and its microenvironment, and characterize extracellular matrix of patients with invasive ductal carcinoma of no special type and lymphatic metastases. Spontaneous and polyclonal activators stimulated production of cytokines by blood immune cells, tumor and its microenvironment were studied in 95 patients with invasive ductal carcinoma of no special type. The concentration of IL-2, IL-4, IL-6, IL-8, IL-10, IL-17, IL-18, IL-1β, IL-1Ra, TNF-α, IFN-γ, G-CSF, GM-CSF, VEGF and MCP-1 was determined by the solid-phase enzyme-linked immunosorbent assay. The condition of fibrous component and presence of neutral glycoproteins and sulfated glycosaminoglycans were evaluated during the research of extracellular matrix. Regional lymphatic metastases were detected in 35 of 95 patients. It was shown that in the presence or absence of lymphatic metastases index of polyclonal activators influence on the production of cytokines by blood immune cells was different for IL-6, IL-8, and IL-1β; while in the case of cytokine production by tumor and its microenvironment the index of influence was different for IL-2 and IL-17. The presence of lymphatic metastases corresponded with the rise of cytokines spontaneous production, while the absence of lymphatic metastases corresponded with the rise of cytokines production stimulated by polyclonal activators. The value of indices of polyclonal activators influence on the production of cytokines by blood immune cells pointed to the highly stimulating effect of polyclonal activators while the value of indices of polyclonal activators influence on cytokines production by tumor and its microenvironments pointed to the low and sometimes even absent effect of polyclonal activators. Basing on these data we propose a ratio of indices of polyclonal activators influence for the better evaluation of the probability of lymphatic metastases during preoperative period. After characterizing extracellular matrix we found out a point threshold, which, in 100% of cases, predicted the presence of lymphatic metastases basing on the condition of extracellular matrix. Using the data acquired, we are proposing a risk group for metastasis among women with no lymphatic metastases in the moment of check-up.

scholarly journals CYTOKINE-PRODUCING RESOURCE OF IMMUNOCOMPETENT BLOOD CELLS IN BREAST TUMORS AND PRECANCEROUS CHANGES OF MAMMARY GLAND

2018 ◽  
Vol 20 (5) ◽  
pp. 681-690
Author(s):  
E. S. Mikhaylova ◽  
N. A. Varaksin ◽  
S. A. Arkhipov ◽  
A. V. Golovanova ◽  
A. A. Studenikina ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Blood Cells ◽  
Cytokine Production ◽  
Ductal Carcinoma ◽  
Gm Csf ◽  
Invasive Ductal Cancer ◽  
Ductal Cancer ◽  
Polyclonal Activator ◽  
Sclerosing Adenosis

At present, only ductal carcinoma in situ is included into the group of precancerous lesions of mammary ducts, according to International Agency for the Study of Cancer. However, based on recent publications, in addition to ductal carcinoma in situ, sclerosing adenosis, intraductal proliferative lesions and radial scar may be also attributed to precancerous changes. A variety of both benign and malignant events in mammary gland, the features of neoplastic growth and age of the patients require new approaches to study of carcinogenic events in mammary gland. As based on the known role of cytokines in genesis of malignancies, the aim of the study was to evaluate the cytokine-producing resource of immunocompetent blood cells in malignant, benign and precancerous mammary disorders. To assess the cytokine-producing resource of immunocompetent blood cells in the patients, we studied quantitative effects of polyclonal activators upon production of cytokines by immunocompetent blood cells of patients with invasive ductal cancer representing a histological type of adenocarcinoma (group I), and patients with non-malignant breast neoplasias (group II). At subsequent step, the patients with non-malignant neoplasms of the breast were divided into a subgroup of patients with only fibroadenoma and mastopathy (group III), and a group which included patients with precancerous diseases, i.e., sclerosing adenosis and interductal proliferates (group IV). Concentrations of IL-2, IL-6, IL-8, IL-10, IL-17, IL-18, IL-1β, IL-1ra, TNFα, IFNγ, G-CSF, GM-CSF, VEGF, and MCP-1 were determined by solid-phase enzyme immunoassay. When comparing groups I and II, we revealed higher influence of polyclonal activators upon production of G-CSF and GM-CSF in patients with invasive ductal cancer. When comparing the influence of polyclonal activation for cytokine production in patients of I and III groups, higher values were registered in patients with invasive ductal cancer (production of IL-2, G-CSF, and GM-CSF), and in patients with fibroadenoma and mastopathy (IL-18, and TNFαproduction). When comparing patients of groups I and IV, higher indexes of the polyclonal activator effects were found only for IL-1ra, G-CSF, and VEGF production in invasive ductal cancer. When comparing the indexes of polyclonal activator influence upon cytokine production of groups III and IV, higher values were obtained in patients with benign changes for the following cytokines: IL-8, IL-18, IL-1β, IL-1ra and TNFα, in contrast to patients with sclerosing adenosis and proliferates. The lower indexes of polyclonal activating effects upon the production of a number of cytokines in patients with precancerous changes, as compared to patients with malignant and benign breast tumors, do not indicate a decreased functional activity of immunocompetent blood cells. However, those may be due to high level of spontaneous cytokine production in sclerosing adenosis and interductal proliferates.

Increase in cytokine production (IL-1β, IL-6, TNF-α but not IFN-γ, GM-CSF or LIF) by stimulated whole blood cells in postmenopausal osteoporosis

Maturitas ◽  
1997 ◽  
Vol 26 (1) ◽  
pp. 63-71 ◽  
Author(s):  
S.X. Zheng ◽  
Y. Vrindts ◽  
M. Lopez ◽  
D. De Groote ◽  
P.F. Zangerle ◽  
...  
Keyword(s):  
Postmenopausal Osteoporosis ◽  
Whole Blood ◽  
Blood Cells ◽  
Cytokine Production ◽  
Gm Csf ◽  
Tnf Α ◽  
Ifn Γ ◽  
Whole Blood Cells

scholarly journals Invasive ductal carcinoma and small lymphocytic lymphoma/chronic lymphocytic leukemia manifesting as a collision breast tumor: A case report and literature review

2021 ◽  
Vol 16 (1) ◽  
pp. 867-871
Author(s):  
Xiaowen Chen ◽  
Jianli Chen ◽  
Sihai Liao ◽  
Yuwen Cao
Keyword(s):  
Chronic Lymphocytic Leukemia ◽  
Invasive Ductal Carcinoma ◽  
Breast Tumor ◽  
Ductal Carcinoma ◽  
Breast Tumors ◽  
Collision Tumor ◽  
Lymphocytic Leukemia ◽  
Epstein Barr Virus Infection ◽  
Small Lymphocytic Lymphoma ◽  
Age Related

Abstract Collision breast tumors, consisting of breast cancer (BC) and non-Hodgkin’s lymphoma (NHL), are extremely rare. Here we report the case of a 64-year-old woman with a collision tumor in her left breast mass that was composed of invasive ductal carcinoma and small lymphocytic lymphoma/chronic lymphocytic leukemia. In addition, we reviewed the published comparable English-language literature. Collision breast tumor composed of BC and NHL is extremely rare. For that reason, there is a lack of consensus about the underlying mechanism, and diagnosing it without delay remains a complex clinical challenge. We found that post-menopausal, age-related estrogen levels changes and Epstein-Barr virus infection are possible pathogenic factors. However, the symptoms are almost identical, and it is difficult to distinguish a simple breast tumor from a breast collision tumor. In this study, we reviewed the clinical features of all patients with BC and NHL colliding breast tumors; this information might enable early identification and prevention of misdiagnosis.

Effect of macrophage-activating factor (GcMAF-RF) upon ex vivo polarization of macrophages, activation of dendritic cells and production of cytokines by human whole blood cells

2021 ◽  
Vol 23 (2) ◽  
pp. 257-274
Author(s):  
S. S. Kirikovich ◽  
E. V. Levites ◽  
E. V. Dolgova ◽  
A. S. Proskurina ◽  
G. S. Ritter ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Whole Blood ◽  
Blood Cells ◽  
Cytokine Production ◽  
Ex Vivo ◽  
Human Whole Blood ◽  
Gm Csf ◽  
Healthy Donors ◽  
Final Maturation ◽  
Whole Blood Cells

This article is the second communication in a series of articles devoted to the effects of a domestic preparation of macrophage-activating factor (GcMAF-RF) and assessment of its biological properties. The aim of this work was to study the effect of the GcMAF-RF upon M0 → M1 polarization of macrophages (Mph), and activation of the professional properties of ex vivo generated antigen-presenting dendritic cells (DC), as well as on ex vivo production of pro-inflammatory (TNFα, IL-1β, IL-6, IFNγ, IL-17, IL-18) and anti-inflammatory (TGF-β, IL-4, IL-10) cytokines, growth factors (IL-2, GM-CSF, G-CSF, VEGF) and chemokines (MCP, IL-8) by the whole blood cells from healthy donors. Mph and DC were generated from the monocytes (3 to 5×106 /ml) derived from adherent fraction of peripheral blood mononuclear cells (MNC) of healthy donors. Granulocyte/macrophage colony-stimulating factor (rhGM-CSF) was used to obtain Mph, whereas DC production was induced by GM-CSF and interferon-α. To provide M1 polarizing signals, bacterial lipopolysaccharide (LPS from E. coli 0114:B4) was used in controls. In experimental series, GcMAF-RF was added 48 h before the end of culture. The stimulating effect of the obtained Mph and DC upon cell proliferation was assessed in allogeneic mixed culture of leukocytes (alloMLC) using radiometric technique, by 3 H-thymidine incorporation. The influence index (IR) of Mph or DC upon allo-SCL was calculated as the ratio of the proliferative response of MNCs in the presence of Mph, or DC to the level of spontaneous MNC proliferation. To determine the cytokine production by human whole blood cells ex vivo, peripheral blood samples from 3 donors with two replicate GcMAF-RF preparations were used, at a total of 6 points. All variants of the study were carried out with mitogen-activated and non-activated blood cells. The cytokine content was determined by the ELISA assays. The effects of GcMAF-RF were quantified as a fold increase (FI), i.e., the ratio of cytokine production in the presence of GcMAF-RF to the level of their spontaneous production. It was shown that the GcMAF-RF preparation was as effective, as lipopolysaccharide (LPS), the standard Mph and DC activator which induces polarization of differentiated M0-macrophages into M1 cells and final maturation of DCs, manifesting by a significant increase in their allo-stimulatory activity in a mixed leukocyte culture (allo-MLC). Moreover, GcMAF-RF stimulates production of numerous cytokines and chemokines (TNFα, IL-1β, IL-6, IL-18, IL-4, IL-10, GM-CSF, G-CSF, VEGF, IL-8), by blood cells (granulocytes, lymphocytes, monocytes), thus indicating direct participation of the macrophage activator GcMAF-RF in various immune processes. The domestic GcMAF-RF drug induces polarization of macrophages M0 → M1, final maturation of DCs and allostimulating activity of Mf and DCs, and is also able to effectively stimulate circulating blood cells to synthesize cytokines/chemokines with pro-inflammatory and immunoregulatory activities. 

Gambaran Histopatologi Tumor Ganas Payudara di Laboratorium Patologi Anatomi RSUP M. Djamil Padang Tahun 2017

2019 ◽  
Vol 1 (1) ◽  
pp. 7-14
Author(s):  
Ana Stesia Suarfi ◽  
Debie Anggraini ◽  
Nurwiyeni Nurwiyeni
Keyword(s):  
Invasive Ductal Carcinoma ◽  
Breast Tissue ◽  
Ductal Carcinoma ◽  
Malignant Tumors ◽  
Histopathological Examination ◽  
Breast Tumors ◽  
Pathology Laboratory ◽  
Cross Sectional ◽  
Anatomy Laboratory ◽  
Malignant Breast

Background: Malignant breast tumors are a disease where there is overgrowth or uncontrolled development of breast tissue cells. The method of mediating malignant breast tumors up to now which is the gold standard is histopathological examination, which in this examination can determine the type of malignant or benign breast tumors. Histopathological examination of breast tissue is needed as a definitive diagnosis in determining the type of breast malignant tumor and the degree of histopathology. Objective: To determine the histopathological picture of malignant breast tumors at the Pathology Anatomy Laboratory of RSUP. M. Djamil Padang in 2017.Method: This research is a descriptive study with cross sectional design. The sample of this study were 47 people with breast malignant tumors at the Anatomy Pathology Laboratory of Dr. M. Djamil Hospital in 2017 which met the inclusion and exclusion criteria. The research data was obtained from the status of the Pathology Anatomy Laboratory at RSUP.DR.M.Djamil Padang in 2017. Results: The highest frequency of malignant breast tumors in the age group 26-65 years (95.7%), female gender (100%), histopathology type invasive ductal carcinoma (63.8%), histopathological degree (68.1%). Conclusion: The description of the incidence of malignant breast tumors in the laboratory of Dr. M.Djamil Hospital Padang in 2017 mostly occurs at the age of 26-65 years, female, histopathological type of invasive ductal carcinoma, degree II.

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