scholarly journals Evaluation and Efficacy of the Antibacterial Activity of Silver and Gold Nanoparticles Synthesize from Camelus dromedarius (Camel) Milk against Oral Pathogenic Bacteria

Author(s):  
Kamini Parmar O.P. Jangir
2020 ◽  
Vol 4 (2) ◽  

Metal nanoparticles possess an extensive scientific and technological significance due to their unique physiochemical properties and their potential applications in different fields like medicine. Silver and gold nanoparticles have shown to have antibacterial and cytotoxic activities. Conventional methods used in the synthesis of the metal nanoparticles involve use of toxic chemicals making them unsuitable for use in medical field. In our continued effort to explore for simple and eco-friendly methods to synthesize the metal nanoparticles, we here describe synthesis and characterization of gold and silver nanoparticles using Gonaderma lucidum, wild non-edible medicinal mushroom. G. lucidum mushroom contain bioactive compounds which can be involved in the reduction, capping and stabilization of the nanoparticles. Antibacterial activity analysis was done on E. coli and S. aureus. The synthesis was done on ultrasonic bath. Characterization of the metal nanoparticles was done by UV-VIS., High Resolution Transmission Electron Microscope (HRTEM) and FTIR. HRTEM analysis showed that both silver and gold nanoparticles were spherical in shape with an average size of 15.82±3.69 nm for silver and 24.73±5.124nm for gold nanoparticles (AuNPs). FTIR analysis showed OH and -C=C- stretching vibrations, an indication of presence of functional groups of biomolecules capping both gold and silver nanoparticles. AgNPs showed inhibition zones of 15.5±0.09mm and 13.3±0.14mm while AuNPs had inhibition zones of 14.510±0.35 and 13.3±0.50mm on E. coli and S. aureus respectively. The findings indicate the potential use of AgNPs and AuNPs in development of drugs in management of pathogenic bacteria.


2013 ◽  
Vol 54 (2) ◽  
pp. 196-202 ◽  
Author(s):  
Palanivel Velmurugan ◽  
Mahudunan Iydroose ◽  
Sang-Myung Lee ◽  
Min Cho ◽  
Jung-Hee Park ◽  
...  

2020 ◽  
pp. 562-570
Author(s):  
Amr M. Abdou ◽  
Riham H. Hedia ◽  
Shimaa T. Omara ◽  
Mai M. Kandil ◽  
M. A. Bakry ◽  
...  

The aim of current study was to isolate and identify naturally occurring probiotic Lactobacillus species in buffalo milk, camel milk, and camel urine to investigate their susceptibility to antibiotics and their antibacterial activity against pathogenic bacteria. A total number of seven samples which included three milk samples from buffalo, three milk samples from camel, and one urine sample from camel were collected and used in this study. The samples were cultured, and 18 isolated strains were identified by using 16S rRNA multiplex Polymerase Chain Reaction analysis, which was performed following DNA extraction from the isolated bacteria. Buffalo and camel milk were different in their Lactobacilli content. All Lactobacilli strains that were found in both camel milk and camel urine, were also found in buffalo milk, Lactobacilli strains in camel milk and urine were generally more resistant to the antibiotic. Lactobacilli isolated from buffalo milk, camel milk, and also camel urine presented variable degrees of antibacterial activity against pathogenic bacteria. Further studies should be conducted with more samples to gain more information in the field of antibacterial activity of probiotic lactobacilli and to understand the mechanisms of their activity. Hopefully, they can be used as natural alternatives instead of synthetic antibiotics.


Author(s):  
Snežana Radisavljević ◽  
Biljana Petrović

Gold nanoparticles (AuNPs) are widely used in biomedical applications, especially diagnostic and drug delivery. The antibacterial activity of nanoparticles depends on the dimensions of the particles. AuNPs may associate with the surface of the cell membrane and cause disorder such as respiration and permeability. The method of binding of particles for bacteria depends on their surface available for interaction. Smaller particles which have the larger surface area available for interaction will show better bactericidal effect than the larger particles. Useful antibacterial agents should also be toxic to various pathogenic bacteria with the ability to coat different surfaces like biomaterials, devices, textiles, food packaging, and so on. The biological and physiochemical properties of synthesized AuNPs have impact on the use of gold nanoparticles like antimicrobial agents, especially for water purification, as well as other biomedical applications.


Nanomaterials ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 819 ◽  
Author(s):  
You Jeong Lee ◽  
Kwangho Song ◽  
Song-Hyun Cha ◽  
Seonho Cho ◽  
Yeong Shik Kim ◽  
...  

Sesquiterpenoids from the flower bud extract of Tussilago farfara were effectively utilized as a reducing agent for eco-friendly synthesis of silver and gold nanoparticles. The silver and gold nanoparticles had a characteristic surface plasmon resonance at 416 nm and 538 nm, respectively. Microscopic images revealed that both nanoparticles were spherical, and their size was measured to be 13.57 ± 3.26 nm for the silver nanoparticles and 18.20 ± 4.11 nm for the gold nanoparticles. The crystal structure was determined to be face-centered cubic by X-ray diffraction. Colloidal stability of the nanoparticle solution was retained in a full medium, which was used in the cell culture experiment. The antibacterial activity result demonstrated that the silver nanoparticles showed better activity (two- to four-fold enhancement) than the extract alone on both Gram-positive and Gram-negative bacteria. Interestingly, the highest antibacterial activity was obtained against vancomycin-resistant Enterococci Van-A type Enterococcus faecium. Cytotoxicity on cancer cell lines confirmed that gold nanoparticles were more cytotoxic than silver nanoparticles. The highest cytotoxicity was observed on human pancreas ductal adenocarcinoma cells. Therefore, both nanoparticles synthesized with the sesquiterpenoids from T. farfara flower bud extract can be applicable as drug delivery vehicles of anticancer or antibacterial agents for future nanomedicine applications.


2015 ◽  
Vol 39 (10) ◽  
pp. 8080-8086 ◽  
Author(s):  
Muhammad Nisar ◽  
Shujaat Ali Khan ◽  
Muhammad Raza Shah ◽  
Ajmal Khan ◽  
Umar Farooq ◽  
...  

The fluoroquinolone drug moxifloxacin (Mox) has been used to protect silver and gold nanoparticles. The nano-conjugates exhibited urease inhibition and antibacterial activity.


2011 ◽  
Vol 60 (1) ◽  
pp. 3-12 ◽  
Author(s):  
ANNA KUREK ◽  
ANNA M. GRUDNIAK ◽  
ANNA KRACZKIEWICZ-DOWJAT ◽  
KRYSTYNA I. WOLSKA

Studies on new antibacterial therapeutics and strategies are currently being conducted in many microbiological, pharmaceutical and biochemical laboratories. The antibacterial activity of plant-derived compounds as well as silver and gold nanoparticles is the subject of this minireview. The application of photodynamic therapy is also discussed.


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