scholarly journals Biophysical Characterization of Chemically Synthesized Antimicrobial Peptide ‘Mastoparan’ and Evaluation of its Activity on Staphylococcus aureus Isolated from Mastitic Milk

2019 ◽  
Vol 8 (11) ◽  
pp. 368-374
Author(s):  
Rajkumar James Singh ◽  
Sameer Shrivastava ◽  
So nal ◽  
Pallavi Shah ◽  
Purnima Gogoi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Peptide ◽  
Biophysical Characterization ◽  
Mastitic Milk

scholarly journals Characterization of hemolytic activity of Staphylococcus aureus strains isolated from bovine mastitic milk

2001 ◽  
Vol 155 (4) ◽  
pp. 339-344 ◽  
Author(s):  
Terhi Ali-Vehmas ◽  
Maija Vikerpuur ◽  
Satu Pyörälä ◽  
Faik Atroshi
Keyword(s):  
Staphylococcus Aureus ◽  
Hemolytic Activity ◽  
Mastitic Milk

scholarly journals Identification and characterization of differentially expressed exosomal microRNAs in bovine milk infected with Staphylococcus aureus

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Shaoyang Ma ◽  
Chao Tong ◽  
Eveline M. Ibeagha-Awemu ◽  
Xin Zhao
Keyword(s):  
Staphylococcus Aureus ◽  
Target Gene ◽  
Protein Transport ◽  
Target Genes ◽  
Bovine Milk ◽  
Differentially Expressed ◽  
Control Group ◽  
Mastitic Milk ◽  
Identification And Characterization

Abstract Background MicroRNAs (miRNAs) in milk-derived exosomes may reflect pathophysiological changes caused by mastitis. This study profiled miRNAs in exosomes from both normal milk and mastitic milk infected by Staphylococcus aureus (S. aureus). The potential targets for differentially expressed (DE) miRNAs were predicted and the target genes for bta-miR-378 and bta-miR-185 were also validated. Results Total RNA from milk exosomes was collected from healthy cows (n = 3, the control group) and S. aureus infected cows (n = 6, the SA group). Two hundred ninety miRNAs (221 known and 69 novel ones) were identified. Among them, 22 known and 15 novel miRNAs were differentially expressed. Target genes of DE miRNAs were significantly enriched in intracellular protein transport, endoplasmic reticulum and identical protein binding. The expression of two miRNAs (bta-miR-378 and bta-miR-185) with high read counts and log2 fold changes (> 3.5) was significantly higher in mastitic milk infected with S. aureus. One target gene (VAT1L) of bta-miR-378 and five target genes (DYRK1B, MLLT3, HP1BP3, NPR2 and PGM1) of bta-miR-185 were validated. Conclusion DE miRNAs in exosomes from normal and S. aureus infected milk were identified. The predicted targets for two DE miRNAs (bta-miR-378 and bta-miR-185) were further validated. The linkage between the validated target genes and diseases suggested that we should pay particular attention to exosome miRNAs from mastitic milk in terms of milk safety.

scholarly journals Identification and characterization of differentially expressed exosomal microRNAs in bovine milk infected with Staphylococcus aureus

2019 ◽  
Author(s):  
Shaoyang Ma ◽  
Chao Tong ◽  
Eveline M. Ibeagha-Awemu ◽  
Xin Zhao
Keyword(s):  
Staphylococcus Aureus ◽  
Target Gene ◽  
Protein Transport ◽  
Target Genes ◽  
Bovine Milk ◽  
Differentially Expressed ◽  
Control Group ◽  
Mastitic Milk ◽  
Identification And Characterization

Abstract Background: MicroRNAs (miRNA) in milk-derived exosomes may reflect pathophysiological changes caused by mastitis. This study profiled miRNAs in exosomes from both normal milk and mastitic milk infected by Staphylococcus aureus (S. aureus). The potential targets for differentially expressed (DE) miRNAs were predicted and the target genes for bta-miR-378 and bta-miR-185 were also validated. Results: Total RNA from milk exosomes was collected from healthy cows (n=3, the control group) and S. aureus infected cows (n=6, the SA group). Two hundred ninety miRNAs (221 known and 69 novel ones) were identified. Among them, 22 known and 15 novel miRNAs were differentially expressed. Target genes of DE miRNAs were significantly enriched in intracellular protein transport, endoplasmic reticulum and identical protein binding. Two miRNAs (bta-miR-378 and bta-miR-185) with high read counts and log 2 fold changes (>3.5) were significantly higher in mastitic milk infected with S. aureus. One target gene (VAT1L) of bta-miR-378 and five target genes (DYRK1B, MLLT3, HP1BP3, NPR2 and PGM1) of bta-miR-185 were validated. Conclusion: DE miRNAs in exosomes from normal and S. aureus infected milk were identified. The predicted targets for two DE miRNAs (bta-miR-378 and bta-miR-185) were further validated. The linkage between the validated target genes and diseases suggested that we should pay particular attention to exosome miRNAs from mastitic milk in terms of milk safety.

scholarly journals Identification and characterization of differentially expressed exosomal microRNAs in bovine milk infected with Staphylococcus aureus

2019 ◽  
Author(s):  
Shaoyang Ma ◽  
Chao Tong ◽  
Eveline M. Ibeagha-Awemu ◽  
Xin Zhao
Keyword(s):  
Staphylococcus Aureus ◽  
Target Gene ◽  
Protein Transport ◽  
Target Genes ◽  
Bovine Milk ◽  
Differentially Expressed ◽  
Control Group ◽  
Mastitic Milk ◽  
Identification And Characterization

Abstract Background: MicroRNAs (miRNA) in milk-derived exosomes may reflect pathophysiological changes caused by mastitis. This study profiled miRNAs in exosomes from both normal milk and mastitic milk infected by Staphylococcus aureus (S. aureus). The potential targets for differentially expressed (DE) miRNAs were predicted and the target genes for bta-miR-378 and bta-miR-185 were also validated. Results: Total RNA from milk exosomes was collected from healthy cows (n=3, the control group) and S. aureus infected cows (n=6, the SA group). Two hundred ninety miRNAs (221 known and 69 novel ones) were identified. Among them, 22 known and 15 novel miRNAs were differentially expressed. Target genes of DE miRNAs were significantly enriched in intracellular protein transport, endoplasmic reticulum and identical protein binding. Two miRNAs (bta-miR-378 and bta-miR-185) with high read counts and log 2 fold changes (>3.5) were significantly higher in mastitic milk infected with S. aureus. One target gene (V AT1L) of bta-miR-378 and five target genes (DYRK1B , MLLT3 , HP1BP3 , NPR2 and PGM1) of bta-miR-185 were validated. Conclusion: DE miRNAs in exosomes from normal and S. aureus infected milk were identified. The predicted targets for two DE miRNAs (bta-miR-378 and bta-miR-185) were further validated. The linkage between the validated target genes and diseases suggested that we should pay particular attention to exosome miRNAs from mastitic milk in terms of milk safety.

Molecular characterization of intercellular adhesion gene in Staphylococcus aureus isolated from bovine mastitic milk

2011 ◽  
Vol 44 (2) ◽  
pp. 247-252 ◽  
Author(s):  
Somanath Kundalik Chavhan ◽  
Dewanand Rajaram Kalorey ◽  
Anshuja Arunkumar Nagdive ◽  
Hemant J. Purohit ◽  
Sukhadeo B. Barbuddhe ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Characterization ◽  
Intercellular Adhesion ◽  
Mastitic Milk

Biophysical Characterization of Thin Films of Zinc Oxide Prepared by Sol-Gel

2021 ◽  
Vol 19 (1) ◽  
pp. 57-61
Author(s):  
Abothur Almohana ◽  
Radea Hateem Jawad
Keyword(s):  
Thin Film ◽  
Thin Films ◽  
Staphylococcus Aureus ◽  
Sol Gel ◽  
Biophysical Characterization ◽  
Force Microscopy ◽  
Nano Powder ◽  
Atomic Force ◽  
Zno Powder

In this research, ZnO nano-powder was used, and the work was in two biological and physical parts. Where the work of the first part was to examine the effect of ZnO powder on Staphylococcus aureus, and the result of inhibition was 100%. As for the second part, the preparation of a thin film by the Sol-Gel method, where thin films were deposited on glass, the structural properties and topography of the surface were studied. The prevailing trend (101). Also, the results of the atomic force microscopy (AFM) tests showed that the resulting granular size of the prepared and annealed films with a temperature (400 ° C) was less than 100 nm, wherein an anti-bacterial nanoscale S. aureus was made.

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