Sero-prevalence of Dirofilaria repens Infection in Dogs by Indirect-ELISA using Microfiarial and Adult Worm Antigen

This study was performed in order to define Schistosoma mansoni antigens that are able to function as modulator agents in the granulomatous hypersensitivity to parasite eggs in BALB/c and C57BL/6 mice. A fraction of S. mansoni, designated PIII, derived from adult worm antigen preparation (SWAP) was obtained using anion-exchange chromatography on an FPLC system. Immunization of mice with PIII in the presence of Corynebacterium parvum and Al(OH)3 as adjuvant induced an immune response in these animals as determined by ELISA and spleen cell proliferation assays against S. mansoni antigens SEA, SWAP and PIII. In addition, PIII caused a significant degree of protection against a challenge infection in immunized mice as observed by the decrease on worm burden recovered from the portal system. We also showed that PIII profoundly inhibited the vigorous anamnestic granulomatous response to eggs in the liver and lungs. This suppression correlated with a significant decrease in granuloma size. From these results we conclude that the PIII preparation contains antigens that can mediate protective anti-parasite immunity and downregulate granulomatous hypersensitivity to S. mansoni eggs.

Download Full-text

Human schistosomiasis mansoni: intensity of infection differentially affects the production of interleukin-10, interferon-γ and interleukin-13 by soluble egg antigen or adult worm antigen stimulated cultures

Transactions of the Royal Society of Tropical Medicine and Hygiene ◽

10.1016/j.trstmh.2003.11.009 ◽

2004 ◽

Vol 98 (9) ◽

pp. 514-519 ◽

Cited By ~ 30

Author(s):

Alda M.S Silveira ◽

Giovanni Gazzinelli ◽

Lúcia F Alves-Oliveira ◽

Jeffrey Bethony ◽

Andrea Gazzinelli ◽

...

Keyword(s):

Adult Worm ◽

Interleukin 10 ◽

Interferon Γ ◽

Intensity Of Infection ◽

Interleukin 13 ◽

Schistosomiasis Mansoni ◽

Human Schistosomiasis ◽

Egg Antigen ◽

Adult Worm Antigen

Download Full-text

Serological Diagnosis of North American Paragonimiasis by Western Blot Using Paragonimus kellicotti Adult Worm Antigen

American Journal of Tropical Medicine and Hygiene ◽

10.4269/ajtmh.12-0720 ◽

2013 ◽

Vol 88 (6) ◽

pp. 1035-1040 ◽

Cited By ~ 20

Author(s):

Peter U. Fischer ◽

Gary J. Weil ◽

Patricia P. Wilkins ◽

Luis A. Marcos ◽

Scott M. Folk ◽

...

Keyword(s):

Western Blot ◽

Adult Worm ◽

North American ◽

Serological Diagnosis ◽

Adult Worm Antigen

Download Full-text

Studies with Brugia pahangi. 16. Precipitation antibody in infected cats detected by counterimmunoelectrophoresis

Journal of Helminthology ◽

10.1017/s0022149x00005241 ◽

1978 ◽

Vol 52 (2) ◽

pp. 127-130 ◽

Cited By ~ 1

Author(s):

R. S. Desowitz ◽

P. Oothuman ◽

D. A. Denham

Keyword(s):

Adult Worm ◽

Brugia Pahangi ◽

Infective Larvae ◽

Precipitin Reaction ◽

Adult Worm Antigen

ABSTRACTIn cats infected with normal, or irradiated, infective (L3) larvae of Brugia pahangi counterimmunoelectro-phoresis revealed the presence of antibody to soluble antigens derived from microfilariae, adults and infective larvae of the same parasite. Infected cats with a persistently high to moderate microfilaraemia gave positive precipitin reactions to L3, microfilarial and adult worm antigens. Cats which had become amicrofilaraemic had antibody to L3 and microfilarial antigens but not to adult worm antigen. Serum from cats inoculated with irradiated L3 larvae produced a precipitin reaction only to the L3 antigen.

Download Full-text

Immunofluorescent studies in filariasis: antibody levels in rats infected with the Malayan forest filaria, Breinlia booliati

Journal of Helminthology ◽

10.1017/s0022149x00006507 ◽

1980 ◽

Vol 54 (2) ◽

pp. 155-160

Author(s):

Mulkit Singh ◽

G. B. Kane ◽

Eu-Hian Yap ◽

Beng-Chuan Ho ◽

Joon-Wah Mak ◽

...

Keyword(s):

Adult Worm ◽

High Antibody ◽

Antibody Technique ◽

Immunofluorescent Antibody ◽

Antibody Levels ◽

Indirect Immunofluorescent ◽

Very High ◽

Adult Worm Antigen

ABSTRACTAntibody levels were investigated in rats infected with Breinlia booliati using the indirect immunofluorescent antibody technique. Sonicated antigens were prepared from microfilariae and adult worms. Adult worm antigen was rather unsatisfactory. The sonicated microfilarial antigen was useful in detecting antibodies in various groups of rat sera. Very high antibody levels were seen in amicrofilaraemic rats harbouring unisexual infections of varying worm loads. The singnificance of these observations are discussed.

Download Full-text