Studies with Brugia pahangi. 16. Precipitation antibody in infected cats detected by counterimmunoelectrophoresis

1978 ◽  
Vol 52 (2) ◽  
pp. 127-130 ◽  
Author(s):  
R. S. Desowitz ◽  
P. Oothuman ◽  
D. A. Denham
Keyword(s):  
Adult Worm ◽  
Brugia Pahangi ◽  
Infective Larvae ◽  
Precipitin Reaction ◽  
Adult Worm Antigen

ABSTRACTIn cats infected with normal, or irradiated, infective (L3) larvae of Brugia pahangi counterimmunoelectro-phoresis revealed the presence of antibody to soluble antigens derived from microfilariae, adults and infective larvae of the same parasite. Infected cats with a persistently high to moderate microfilaraemia gave positive precipitin reactions to L3, microfilarial and adult worm antigens. Cats which had become amicrofilaraemic had antibody to L3 and microfilarial antigens but not to adult worm antigen. Serum from cats inoculated with irradiated L3 larvae produced a precipitin reaction only to the L3 antigen.

Delayed macrofilaricidal activity of diethylcarbamazine against Brugia pahangi in Mongolian jirds

2004 ◽  
Vol 78 (4) ◽  
pp. 293-295 ◽  
Author(s):  
Y. Fujimaki ◽  
P. Sithithaworn ◽  
Y. Mitsui ◽  
Y. Aoki
Keyword(s):  
Adult Worm ◽  
Post Infection ◽  
Recovery Rate ◽  
Treated Group ◽  
Post Treatment ◽  
Brugia Pahangi ◽  
Infective Larvae ◽  
Final Treatment ◽  
Antifilarial Activity

AbstractThe macrofilaricidal activity of diethylcarbamazine (DEC) was confirmed in jirds infected with Brugia pahangi. Seventy jirds were inoculated subcutaneously with 100 infective larvae. At 20 weeks post-infection, the microfilaraemic jirds were divided into two groups, untreated and treated. For the treated group, 200 mg kg−1 of DEC was injected intraperitoneally for 5 consecutive days. One, 4, 8, 12, 16 and 27 weeks after the final treatment, 4–7 jirds in each group were sacrificed to measure adult worm burdens. The number of adult worms recovered from treated jirds was comparable to controls at earlier necropsy (1 and 4 weeks post-treatment). However, at late necropsy (8 weeks and later) the recovery rate of adult worms in treated jirds was significantly lower than that in untreated controls, indicating an adultcidal effect of DEC. The present study demonstrates that DEC requires 8 weeks to kill B. pahangi adult worms in jirds and that the Mongolian jird is a useful model for screening antifilarial activity.

Attempted vaccination of jirds (Meriones unguiculatus) againstBrugia pahangiwith radiation attenuated infective larvae

1986 ◽  
Vol 60 (2) ◽  
pp. 149-155 ◽  
Author(s):  
W. Chusattayanond ◽  
D. A. Denham
Keyword(s):  
Meriones Unguiculatus ◽  
Brugia Pahangi ◽  
Infective Larvae

ABSTRACTJirds were vaccinated by three to five subcutaneous (SC) injections of infective larvae ofBrugia pahangiwhich had been irradiated at 25, 45 or 90 krads from a60Co source. They were challenged either SC or intraperitoneally. Vaccination with four doses of 50 larvae irradiated with 25 krads produced 49·3% resistance to IP challenge worms and 39·8% against SC challenge worms. Five doses of larvae irradiated with 45 krads produced 62% resistance to SC challenge. Three doses of larvae irradiated with 90 krads produced 74·9% resistance to SC challenge and five doses produced 76·2% resistance. The reasons why irradiated larvae produce resistance whereas normal larvae do not are discussed.

scholarly journals In vitro and in vivo anthelmintic activity of extracts from Artemisia parviflora and A. sieversiana

2017 ◽  
Vol 54 (3) ◽  
pp. 218-224 ◽  
Author(s):  
S. Irum ◽  
H. Ahmed ◽  
B. Mirza ◽  
K. Donskow-Łysoniewska ◽  
A. Muhammad ◽  
...  
Keyword(s):  
Adult Worm ◽  
Plant Extracts ◽  
Small Ruminants ◽  
Anthelmintic Activity ◽  
Gastrointestinal Nematodes ◽  
Economic Losses ◽  
Egg Hatching ◽  
Infective Larvae

SummaryIn the northern areas of Pakistan, the use of Artemisia based therapeutics is a common practice. Plants of genus Artemisia are known to possess anthelmintic and therapeutic effect. Infections caused by gastrointestinal nematodes are major threat to livestock industry across the world resulting in loss of production and indirect economic losses due to high cost of anthelmintic drugs. Present study was carried out to evaluate in vitro and in vivo effect of Artemisia sieversiana and Artemisia parviflora on Haemonchus contortus, a parasitic nematode of small ruminants. Methanolic plant extract was tested against three different developmental stages using an egg hatch assay, infective larvae and adult worm motility assay. Different concentrations were used for the bioassays and post exposure mortality was recorded after 8 hr for adult worms and infective larvae, while egg inhibition percentage was observed after 27 hr. A highly significant ability to inhibit the egg hatching (100 %) was recorded for both plant extracts while, the highest activity for adult worm assay and larvicidal assay was 90 % for A. sieversiana. The highest activity for adult motility and larvicidal assay for A. parviflora was 89 % and 86.6 % respectively. For in vivo trials maximum parentage reduction was 77.0 % for A. sieversiana and 73.6 % for A. parviflora. It is concluded that selected plant extracts were effective in reducing worm burden in animals.

Acquired Resistance to Fasciola Hepatica in Cattle Using a Purified Adult Worm Antigen

1987 ◽  
Vol 37 (2) ◽  
pp. 362-369 ◽  
Author(s):  
George V. Hillyer ◽  
El Tahir M. Haroun ◽  
Anabel Hernandez ◽  
Maricelis Soler De Galanes
Keyword(s):  
Adult Worm ◽  
Fasciola Hepatica ◽  
Acquired Resistance ◽  
Adult Worm Antigen

The Influence of Seitz Filtration on the Activity of Schistosoma mansoni (Adult Worm) Antigen

1960 ◽  
Vol 46 (5) ◽  
pp. 647 ◽  
Author(s):  
Bobby J. Neal ◽  
J. Pellegrino ◽  
Benedito A. Rodrigues
Keyword(s):  
Schistosoma Mansoni ◽  
Adult Worm ◽  
Adult Worm Antigen

Studies on Filariasis. I.—The Miģration of the Infective Larvae of Brugia pahangi in Aedes togoi and their Loss from the Mosquito under Experimental Conditions

1966 ◽  
Vol 40 (3-4) ◽  
pp. 343-362 ◽  
Author(s):  
M. M. J. Lavoipierre ◽  
Beng Chuan Ho
Keyword(s):  
Blood Meal ◽  
Significant Loss ◽  
Experimental Conditions ◽  
Brugia Pahangi ◽  
Infective Larvae ◽  
Aedes Togoi

1. There is a slow but definite loss of infective forms of B. pahangi from A. togoi maintained on honey and water alone and denied access to a host.2. During the first blood meal after maturation of the filarial larvae there is a highly significant loss of infective forms from the mosquitos. On the 13th day, when they concentrate in the head of the mosquito, there is an unusually high (96·2%) loss after bloodfeeding.3. The infective larvae migrate to and from the head and proboscis, and the thorax. There is also a migration to and from the head and the proboscis. This shows itself as a rhythm with 8 day oscillations.

Possible direct effect of diethylcarbamazine on the infective larvae of Brugia pahangi

1990 ◽  
Vol 64 (4) ◽  
pp. 295-301
Author(s):  
Yasunori Fujimaki ◽  
Masaaki Shimada ◽  
Yoshinori Mitsui ◽  
Eisaku Kimura ◽  
Yoshiki Aoki
Keyword(s):  
Survival Rate ◽  
Culture System ◽  
Direct Action ◽  
Morphological Characteristics ◽  
Post Inoculation ◽  
Brugia Pahangi ◽  
Infective Larvae ◽  
Rate Of Recovery ◽  
Foetal Bovine Serum

ABSTRACTThe direct action of diethylcarbamazine (DEC) on the infective larvae of Brugia pahangi was studied. The larvae were cultured in RPMI 1640 supplemented with foetal bovine serum and antibiotics for 22 days. Most of the larvae remained alive for 8 days, but survival rate of larvae decreased rapidly from day 10 onwards. The larvae did not grow in the culture system. The addition of DEC did not affect the morbidity of the larvae and no difference was observed in the morphological characteristics between the larvae cultured in the presence or absence of DEC.The infective larvae were cultured in vitro for 5 days in the presence or absence of DEC, and inoculated into jirds. The animals were necropsied at intervals, and developing larvae and adult worms were recovered. When the larvae were cultured without DEC and then inoculated subcutaneously into jirds, 29.8% of the inoculum was recovered 3–15 days, and 25% 19–22 weeks, post-inoculation. However, when the larvae were exposed to DEC in vitro and inoculated into jirds, the rate of recovery was reduced to 25% 3–15 days post-inoculation and 2% after 19–22 weeks. When the control larvae cultured in vitro were inoculated intraperitoneally into jirds, 41·3% of inoculum was recovered 3–15 days, and 42·8% 19–22 weeks, post-inoculation. Again the corresponding value for larvae exposed to DEC in vitro was reduced to 19.8% 3–15 days, and 8% 19–22 weeks, post-inoculation. It was observed that the larvae exposed to DEC in vitro were retarded in their development in jirds. These results indicate that DEC has a direct action against the infective larvae of B. pahangi.

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