Human schistosomiasis mansoni: intensity of infection differentially affects the production of interleukin-10, interferon-γ and interleukin-13 by soluble egg antigen or adult worm antigen stimulated cultures

The circumoval precipitin test (COPT), enzyme-linked immunosorbent assay (ELISA) and the immunoblotting anti-adult worm antigen (AWA) and soluble egg antigen (SEA) tests were applied to 17 chronically schistosome-infected patients for the detection of anti-Schistosoma mansoni antibodies before and on four occasions after oxamniquine administration over a period of six months. Compared to a control group, schistosomiasis patients showed high levels of IgG antibodies in AWA and SEA-ELISA. A decrease in IgG levels was observed six months after treatment, although negative reactions were not obtained. Significant decreases in IgG1, IgG3 and, mainly, IgG4, but not anti-SEA IgG2 levels were observed six months after treatment, again without negativity. Analysis of anti-AWA IgG antibodies by immunoblotting before treatment showed a 31 kDa strand in 14 patients (82%) which disappeared in three cases up to six months after treatment; furthermore, anti-SEA IgG antibodies showed the same band in nine patients (53%) before treatment, which disappeared in only four cases up to six months after treatment.

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Cytokine Production in Acute versus Chronic Human Schistosomiasis Mansoni: The Cross‐Regulatory Role of Interferon‐γ and Interleukin‐10 in the Responses of Peripheral Blood Mononuclear Cells and Splenocytes to Parasite Antigens

The Journal of Infectious Diseases ◽

10.1086/314748 ◽

1999 ◽

Vol 179 (6) ◽

pp. 1502-1514 ◽

Cited By ~ 76

Author(s):

Silvia M. L. Montenegro ◽

Paulo Miranda ◽

Siddhartha Mahanty ◽

Frederico G. C. Abath ◽

Kirte M. Teixeira ◽

...

Keyword(s):

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Cytokine Production ◽

Interleukin 10 ◽

Interferon Γ ◽

Peripheral Blood Mononuclear ◽

Schistosomiasis Mansoni ◽

Blood Mononuclear Cells

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TGF-β and IL-10 regulation of IFN-γ produced in Th2-type schistosome granulomas requires IL-12

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.2001.281.4.g940 ◽

2001 ◽

Vol 281 (4) ◽

pp. G940-G946 ◽

Cited By ~ 3

Author(s):

Khurram Qadir ◽

Ahmed Metwali ◽

Arthur M. Blum ◽

Jie Li ◽

David E. Elliott ◽

...

Keyword(s):

Transforming Growth Factor ◽

Transforming Growth Factor Β ◽

Interleukin 10 ◽

Interferon Γ ◽

T Helper 2 ◽

Subunit Mrna ◽

Cba Mice ◽

Neutralizing Monoclonal Antibody ◽

Ifn Γ ◽

Egg Antigen

Interleukin-10 (IL-10) and transforming growth factor-β (TGF-β) regulate CD4+T cell interferon-γ (IFN-γ) secretion in schistosome granulomas. The role of IL-12 was determined using C57BL/6 and CBA mice. C57BL/6 IL-4 −/− granuloma cells were stimulated to produce IFN-γ when cultured with IL-10 or TGF-β neutralizing monoclonal antibody. In comparison, C57BL/6 wild-type (WT) control granuloma cells produced less IFN-γ. IL-12, IL-18, and soluble egg antigen stimulated IFN-γ release from C57BL/6 IL-4 −/− and WT mice. IFN-γ production in C57 IL-4 −/− and WT granulomas was IL-12 dependent, because IL-12 blockade partly abrogated IFN-γ secretion after stimulation. All granuloma cells released IL-12 (p70 and p40), and IL-12 production remained constant after anti-TGF-β, anti-IL-10, recombinant IL-18, or antigen stimulation. C57 WT and IL-4 −/− mouse granuloma cells expressed IL-12 receptor (IL-12R) β1-subunit mRNA but little β2 mRNA. TGF-β or IL-10 blockade did not influence β1 or β2 mRNA expression. CBA mouse dispersed granuloma cells released no measurable IFN-γ, produced IL-12 p70 and little p40, and expressed IL-12R β2 and little β1 mRNA. In T helper 2 (Th2) granulomas of C57BL/6 WT and IL-4 −/− mice, cells produce IL-12 (for IFN-γ production) and IL-10 and TGF-β modulate IFN-γ secretion via mechanisms independent of IL-12 and IL-12R mRNA regulation. We found substantial differences in control of granuloma IFN-γ production and IL-12 circuitry in C57BL/6 and CBA mice.

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Zelluläre Interferon-γ und Interleukin-13 Immunantwort bei Typ 1, Typ 2 und Latentem Autoimmune Diabetes

Diabetologie und Stoffwechsel ◽

10.1055/s-0032-1314649 ◽

2012 ◽

Vol 7 (S 01) ◽

Author(s):

A Strom ◽

B Menart ◽

MC Simon ◽

MN Pham ◽

H Kolb ◽

...

Keyword(s):

Autoimmune Diabetes ◽

Interferon Γ ◽

Interleukin 13

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Serum levels of sCD23, interleukin-10 and interferon-γ in patients with coeliac disease

Journal of Gastroenterology and Hepatology ◽

10.1111/j.1440-1746.1997.tb00535.x ◽

1997 ◽

Vol 12 (9-10) ◽

pp. 685-689 ◽

Cited By ~ 2

Author(s):

AS BANSAL ◽

J BRUCE ◽

A THOMSON ◽

P KERLIN

Keyword(s):

Coeliac Disease ◽

Serum Levels ◽

Interleukin 10 ◽

Interferon Γ

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Immunoprecipitins in human schistosomiasis detected with adult worm antigens released by 3M KC1

Journal of Helminthology ◽

10.1017/s0022149x00007276 ◽

1977 ◽

Vol 51 (1) ◽

pp. 71-72 ◽

Cited By ~ 3

Author(s):

Mauro Scapin ◽

Miriam Tendler

Keyword(s):

Schistosoma Mansoni ◽

Adult Worm ◽

Calcium Chloride ◽

Surface Proteins ◽

Immunochemical Characterization ◽

Human Schistosomiasis

Several methods are being applied for solubilizing adult Schistosoma mansoni antigens which may allow their immunochemical characterization and purification. Studies on antigens and immunoprecipitins in schistosome infections have been carried out with homogenized extracts of adult worms and/or larval forms of Schistosoma mansoni in saline (Biguet et al., 1962; Kagan and Norman, 1963; Silva and Ferri, 1965), or water (Kent, 1963). Kusel (1972) studied surface proteins in S. mansoni membrane, treating the worms with 0·5% saponin in 3% calcium chloride. Murrel et al. (1974) compared by immunodiffusion a crude culture antigen with extracts obtained by freeze-thawing of adult worms and by treatment in 3M KCl.

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