scholarly journals UJI AKTIVITAS ANTIBAKTERI DAN FITOKIMIA KULIT BAWANG MERAH (Allium cepa L.) HASIL EKSTRAKSI METODE Microwave Assisted Extraction (MAE)

2018 ◽  
Vol 11 (2) ◽  
pp. 71
Author(s):  
Fitria Dewi Sulistiyono ◽  
Trirakhma Sofihidayati ◽  
Bina Lohitasari

Ekstrak kulit bawang merah mengandung flavonoid, polifenol, saponin, terpenoid dan alkaloid. Metode ekstraksi modern dengan memanfaatkan radiasi gelombang mikro yang disebut dengan MAE (Microwave Assisted Extraction) belum banyak dilakukan. Metode MAE terbukti lebih efektif karena pemanasan pelarut secara cepat dan efisien dibandingkan metode ekstraksi secara konvensional. Tujuan penelitan ini adalah mengetahui senyawa fitokimia dan aktivitas ekstrak kulit bawang merah dengan metode MAE pada Staphylococcus aureus. Deteksi senyawa fitokimia dilakukan dengan metode kualitatif, yaitu ada tidaknya senyawa flavonoid, saponin, alkaloid dan tanin. Aktivitas ekstrak kulit bawang merah terhadap S.aureus dilakukan dengan metode kertas cakram dengan melihat zona bening/hambat yang dihasilkan. Konsentrasi ekstrak yang digunakan adalah 5, 10, 15, 20 dan 25% (b/v). Hasil penelitian menunjukkan bahwa ektrak kulit bawang merah mempunyai senyawa flavonoid, saponin dan tanin. Ekstrak kulit bawang merah dapat menghambat pertumbuhan S.aureus ditunjukkan dengan adanya zona bening dari kosentrasi 5, 10, 15, 20 dan 25% (b/v) adalah 14; 15,5; 16; 19; 19,5 mm. Hasil tersebut menunjukan bahwa MAE lebih efektif daripada metode konvensional (maserasi) dalam menghambat S. aureus. Berdasarkan hasil aktivitas antibakteri, ekstrak kulit bawang merah dapat dimanfaatkan dalam dunia industri sebagai salah satu komponen produk pencegahan infeksi bakteri.   Onion skin extract containing flavonoid , catakin , saponin , terpenoid and alkaloid . Microwave Assisted Extraction (MAE) is modern method with microwave radiation. MAE have proven more effective because a solvent warming quickly and more efficient compared other methode (maseration). This study aims to explore the phytochemical activity presenced in onion skin extract. that was obtained by the Microwave Assited Extraction (MAE) method and to evaluate the antibacterial activity against Staphilococcus aureus. The phytochemical screening of onion skin extracted by using MAE revealed the presence of alkaloids, saponins, tannins and flavonoids. The antibacterial activity of onion skin extract was carried out against S. aureus by measuring the diameter of bacterial growth inhibition zones through the diffusion method. The results of qualitative phytochemical screening tests on onion skin extract shows that the positive sample contains alkaloids, saponins, tannins and flavonoids. Range of onion skin extract concentration used in this research were 5, 10, 15, 20, and 25% w/v. The inhibition zone of extract at concentration 5, 10, 15, 20, and 25% w/v were 14.00; 15.50; 16.00; 19.00; and 19.50 mm respectively. Base on antibacterial result, onion skin extract can be used as one ingredient in the manufacture of antibacterial products.

2020 ◽  
Vol 2 (1) ◽  
pp. 102
Author(s):  
Yanu Andhiarto ◽  
Rina Andayani ◽  
Nur Hidayatul Ilmiyah

Skin infections most often found in developing countries, especially in the tropics, are purulent skin inflammation (pyoderma), the main cause of Staphylococcus aureus. The increasing number of cases of bacteria that are resistant to antibacterial can encourage the extraction of sources of antibacterial drugs from natural ingredients. One of the plants that is thought to have the antibacterial activity of Staphylococcus aureus is the Neem plant. In this study an antibacterial activity test was carried out with the well diffusion method from 96% ethanol extract of Mimba leaves extracted by percolation method. Previously 96% ethanol extract from Mimba leaves had been identified by secondary metabolites by phytochemical screening and standardized extract testing. Standarization using non-specific parameters (moisture content, ash content and drying losses) found that 96% ethanol extract of Mimba leaves entered a range of standard parameters and in phytochemical screening tests obtained 96% ethanol extract of Mimba leaves containing alcaloid, saponins, tannins, steroids / terpenoids and flavonoids. The results of the study on the antibacterial activity test showed a concentration of 75% ethanol extract of 96% Mimba leaves classified as having strong activity, at a concentration of 50% and 25% classified as having moderate activity. Based on this study it can be concluded that the three concentrations of 96% ethanol extract of Mimba leaves were able to inhibit the growth of Staphylococcus aureus bacteria.


2010 ◽  
Vol 30 (5) ◽  
pp. 567-568
Author(s):  
Xiao-li LI ◽  
Ming-yuan ZHANG ◽  
Wei-quan ZHAO ◽  
Man Li ◽  
Hai-ying TENG ◽  
...  

2015 ◽  
Vol 11 (3) ◽  
pp. 142-145 ◽  
Author(s):  
Simone Carradori ◽  
Luisa Mannina ◽  
Federica De Cosmi ◽  
Tamara Beccarini ◽  
Daniela Secci ◽  
...  

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