scholarly journals Reference materials of catalytic activity as a means of ensuring the metrological traceability of measurement results. KATAL

2019 ◽  
Vol 14 (1-2) ◽  
pp. 25-32
Author(s):  
E. V. Kulyabina ◽  
O. N. Melkova ◽  
E. A. Guskova ◽  
T. V. Grebennikova
Keyword(s):  
Catalytic Activity ◽  
Bacillus Anthracis ◽  
Reference Materials ◽  
Mass Concentration ◽  
Ebola Virus ◽  
Protective Antigen ◽  
Metrological Traceability ◽  
Measurement Results ◽  
Traceability Of Measurement Results ◽  
Recombinant Protective Antigen

Introduction. The article considers the problems of metrological support of catalytic activity measurements. The paper gives certain characteristics of the State Primary Special Measurement Standard for the unit of catalytic activity – KATAL and examines the role of reference materials (RMs) in ensuring the metrological traceability of measurement results.Materials and methods. A method for measuring the concentration of the recombinant protective antigen of Bacillus anthracis and the recombinant GP-protein of the Ebola virus, which means in using «sandwich» enzyme-linked immunosorbent assay, was certified by FGUP «VNIIMS» as a measurement procedure. The following RMs tested by FGUP «VNIIMS» can be used for the development and production of appropriate control samples when performing measurements for comparisons: the RM for the mass concentration of the recombinant protective antigen of Bacillus anthracis in phosphate-saline solution, the RM for the mass concentration of the recombinant GP-protein of the Ebola virus, and the RM for the mass concentration of recombinant Clostridium Difficile toxin.Results. The paper presents the main reactions of the method for measuring the catalytic activity of catalysts for heterogeneous processes, which are also used to carry out exhaust gas cleaning processes.Discussion and conclusions. Thus, conditions have been created for building hierarchies of calibrations of in-demand objects established on the basis of the List of Critical Technologies of the Russian Federation, state programs for the development of industrial sectors.

Problems of ensuring metrological traceability of measurement results of indicators of quality for food products and food raw materials

2020 ◽  
pp. 64-70
Author(s):  
Mariya Y. Medvedevskikh ◽  
Anna S. Sergeeva
Keyword(s):  
Reference Materials ◽  
Mass Fraction ◽  
Nutritional Value ◽  
Animal Feed ◽  
Raw Materials ◽  
Meat Products ◽  
Metrological Traceability ◽  
Food Products ◽  
Freeze Dried ◽  
Measurement Results

The article raises the problem of ensuring metrological traceability of the measurement results of indicators of quality and nutritional value for food products and food raw materials: water (moisture), nitrogen (protein, crude protein), fat, ash and carbohydrates. The problem under consideration can be solved by applying reference materials of food composition, traceable to state primary measurement standards GET 173-2017 and GET 176-2019 and primary reference measurement procedures (PRMP), for attestation of measurement procedures and accuracy checking of measurement results. The article discusses the results of the PRMP development of mass fraction of fat, ash and carbohydrates in food products and food raw materials, as well as mass fraction of crude fat (oil content) in oil crops seeds and products based on them. The paper also presents metrological characteristics of reference materials of composition of dry dairy products, grain-milk dry porridges for nutrition of babies, grain dry porridges for nutrition of babies, egg powder, freeze-dried meat products, animal feed. The results of the work allow for building a chain of metrological traceability from GET 173-2017, GET 176-2019 and PRMP to routine measurement procedures, thereby ensuring the uniformity of measurements of nutritional value of food products.

scholarly journals Attenuated Nontoxinogenic and Nonencapsulated Recombinant Bacillus anthracis Spore Vaccines Protect against Anthrax

2000 ◽  
Vol 68 (8) ◽  
pp. 4549-4558 ◽  
Author(s):  
S. Cohen ◽  
I. Mendelson ◽  
Z. Altboum ◽  
D. Kobiler ◽  
E. Elhanany ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Protective Immunity ◽  
Protective Antigen ◽  
Cell Vaccine ◽  
Lethal Challenge ◽  
Electrospray Mass Spectroscopy ◽  
Immunological Tests ◽  
Anthrax Spores ◽  
Biochemical Analyses ◽  
Recombinant Protective Antigen

ABSTRACT Several highly attenuated spore-forming nontoxinogenic and nonencapsulated Bacillus anthracis vaccines differing in levels of expression of recombinant protective antigen (rPA) were constructed. Biochemical analyses (including electrospray mass spectroscopy and N terminus amino acid sequencing) as well as biological and immunological tests demonstrated that the rPA retains the characteristics of native PA. A single immunization of guinea pigs with 5 × 107 spores of one of these recombinant strains, MASC-10, expressing high levels of rPA (≥100 μg/ml) from a constitutive heterologous promoter induced high titers of neutralizing anti-PA antibodies. This immune response was long lasting (at least 12 months) and provided protection against a lethal challenge of virulent (Vollum) anthrax spores. The recombinant B. anthracis spore vaccine appears to be more efficacious than the vegetative cell vaccine. Furthermore, while results clearly suggest a direct correlation between the level of expression of PA and the potency of the vaccine, they also suggest that some B. anthracisspore-associated antigen(s) may contribute in a significant manner to protective immunity.

scholarly journals The requirements of the international standards on traceability of measurement results at OJSC «BSW – Management Company of Holding «BMC»

2019 ◽  
pp. 70-72
Author(s):  
E. V. Borisenko ◽  
I. V. Buyanova
Keyword(s):  
Metrological Traceability ◽  
International Standards ◽  
Measuring Instruments ◽  
International Level ◽  
Product Testing ◽  
Management Company ◽  
Uniformity Of Measurements ◽  
Effective Functioning ◽  
Measurement Results ◽  
Traceability Of Measurement Results

A serious barrier to the sale of products on the market is the trust in organizations that perform measurements during product testing, as well as calibration (verification) of the used measuring instruments. Confidence and recognition of measurement results at the international level is ensured through an accreditation mechanism. To ensure effective functioning at the international level, accreditation organizations enter into a multilateral agreement that provides for the use of common standards in the conduct of accreditation. One of the important requirements of these standards is the implementation and demonstration of metrological traceability, which is a tool for ensuring the uniformity of measurements.

Problems of ensuring the uniformity of blood pressure measurements

Metrologiya ◽  
2020 ◽  
pp. 46-71
Author(s):  
V. F. Romanovskiy ◽  
A. M. Romanovskaya ◽  
E. A. Nenasheva
Keyword(s):  
Blood Pressure ◽  
Pulse Wave ◽  
Metrological Traceability ◽  
The Body ◽  
Pressure Measurements ◽  
Wave Method ◽  
Invasive Blood Pressure ◽  
Non Invasive ◽  
Measurement Results ◽  
Traceability Of Measurement Results

The problems of ensuring the unity of non-invasive blood pressure measurements are considered. It is shown that the artery and surrounding tissues of the body serve as a means of comparing the values of blood pressure and air pressure in the cuff, and that the metrological traceability of measurement results to pressure standards only partially determines the reliability of these results. The potential possibilities of the surface pulse wave method in comparison with the Korotkov tone method are estimated.

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