Structural Insights into Thermotoga maritima FtsH Periplasmic Domain on Substrate Recognition

2016 ◽  
Author(s):  
Jun Yop An ◽  
Humayun Sharif ◽  
Gil Bu Kang ◽  
Kyung Jin Park ◽  
Jung-Gyu Lee ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Protein Complexes ◽  
Thermotoga Maritima ◽  
Substrate Recognition ◽  
Hydrophobic Membrane ◽  
Membrane Protein Complexes ◽  
Periplasmic Domain ◽  
Ph Dependent ◽  
Structural Insights

Prompt removal of misfolded membrane proteins and misassembled membrane protein complexes is essential for membrane homeostasis. However, the elimination of these toxic proteins from the hydrophobic membrane environment has high energetic barriers. Transmembrane FtsH is the only known ATP-dependent protease responsible for this task, unlike other well-studied soluble ATP-dependent proteases. The mechanisms by which FtsH recognizes, unfolds, translocates, and proteolyzes its substrates remain unclear. Here, we report the crystal structures of the Thermotoga maritima FtsH periplasmic domain (PD) in an associative trimeric state at a 1.5-1.95 Å resolution. We also describe the pH-dependent oligomerization states of the isolated PD using dynamic light scattering. These observations help us understand how FtsH recognizes membrane-anchored misfolded proteins.

Structural insights into the oligomerization of FtsH periplasmic domain from Thermotoga maritima

2018 ◽  
Vol 495 (1) ◽  
pp. 1201-1207
Author(s):  
Jun Yop An ◽  
Humayun Sharif ◽  
Gil Bu Kang ◽  
Kyung Jin Park ◽  
Jung-Gyu Lee ◽  
...  
Keyword(s):  
Thermotoga Maritima ◽  
Periplasmic Domain ◽  
Structural Insights

pH-dependent oligomerization of BPTI in undersaturated and supersaturated solutions studied by dynamic light scattering

2002 ◽  
Vol 237-239 ◽  
pp. 289-294 ◽  
Author(s):  
Shinpei Tanaka ◽  
Mitsuo Ataka ◽  
Kazuo Onuma ◽  
Jean-Pierre Astier ◽  
Stéphane Veesler
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Ph Dependent ◽  
Supersaturated Solutions

scholarly journals Calix[4]arene-click-cyclodextrin and supramolecular structures with watersoluble NIPAAM-copolymers bearing adamantyl units: “Rings on ring on chain”

2010 ◽  
Vol 6 ◽  
pp. 784-788 ◽  
Author(s):  
Bernd Garska ◽  
Monir Tabatabai ◽  
Helmut Ritter
Keyword(s):  
Mass Spectrometry ◽  
Light Scattering ◽  
Ir Spectroscopy ◽  
Complex Formation ◽  
Dynamic Light Scattering ◽  
Click Reaction ◽  
Supramolecular Complex ◽  
H Nmr ◽  
Microwave Assisted ◽  
Ph Dependent

We describe the calixarene-cyclodextrin-coupling via click reaction starting from 5,11,17,23-tetra-tert-butyl-25,27-dipropargylether-26,28-hydroxy-calix[4]arene (calix[4]arene-dipropargylether) (2) onto 6I-azido-6I-deoxycyclomaltoheptaose (3) under microwave assisted conditions. The coupling was proven by MALDI-TOF mass spectrometry, 1H NMR and IR-spectroscopy. The pH dependent supramolecular complex formation with poly(NIPAAM) bearing attached adamantyl units was investigated by dynamic light scattering (DLS) and turbidity measurements.

Tuning of Silica Nanoparticles-Lysozyme Protein Complexes in presence of SDS Surfactant

Soft Matter ◽  
2021 ◽  
Author(s):  
Debasish Saha ◽  
Sugam Kumar ◽  
Debes Ray ◽  
Jitendra Mata ◽  
Andrew Edward Whitten ◽  
...  
Keyword(s):  
Light Scattering ◽  
Sodium Dodecyl Sulfate ◽  
Dynamic Light Scattering ◽  
Silica Nanoparticles ◽  
Anionic Surfactant ◽  
Protein Complexes ◽  
Sodium Dodecyl ◽  
Cationic Protein ◽  
Dodecyl Sulfate ◽  
Sds Surfactant

The structure of complexes of anionic silica nanoparticles (size16 nm)-lysozyme (cationic) protein, tuned by the addition of anionic surfactant sodium dodecyl sulfate (SDS), has been investigated by dynamic light scattering...

scholarly journals Controlled Release of Doxorubicin from the Drug Delivery Formulation Composed of Single-Walled Carbon Nanotubes and Congo Red: A Molecular Dynamics Study and Dynamic Light Scattering Analysis

Pharmaceutics ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 622 ◽  
Author(s):  
Anna Jagusiak ◽  
Katarzyna Chlopas ◽  
Grzegorz Zemanek ◽  
Pawel Wolski ◽  
Tomasz Panczyk
Keyword(s):  
Carbon Nanotubes ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Congo Red ◽  
Binding Capacity ◽  
Single Walled Carbon Nanotubes ◽  
Carrier System ◽  
Ph Changes ◽  
Ph Dependent ◽  
Walled Carbon Nanotubes

The controlled delivery and release of drug molecules at specific targets increases the therapeutic efficacy of treatment. This paper presents a triple complex which is a new potential drug delivery system. Triple complex contains single-walled carbon nanotubes, Congo red, and doxorubicin. Nanotubes are built of a folded graphene layer providing a large surface for binding Congo red via “face-to-face” stacking which markedly increases the binding capacity of the carrier. Congo red is a compound that self-associates to form supramolecular ribbon-like structures, which are able to bind some drugs by intercalation. The nanotube–Congo red complex can bind the model drug doxorubicin. Thus, a new triple carrier system was obtained. The aim of this paper is to present studies on the controlled release of a model anticancer drug from a triple carrier system through pH changes. The specific aim of the study was to model the structure of the obtained experimental systems and to compare the changes in the average energy of interaction between its components induced by pH changes. The studies also aimed to compare the intensity of pH-dependent changes in hydrodynamic diameters of individual components of the triple carrier system. The effect of pH changes on the stability of the analyzed systems was examined using the molecular modeling method and dynamic light scattering. The decrease in pH influenced the structure and stability of the analyzed triple systems and ensured efficient drug release. The changes in hydrodynamic diameters of the obtained fractions were examined with the use of dynamic light scattering and were confirmed by computer simulation methods. The formulation presented in this paper shows potential for a therapeutic application owing to its high drug binding capacity and pH-dependent release. This ensures prolonged local action of the drug. The results reveal that the studied complex fulfills the basic requirements for its potential use as drug carrier, thus reducing side effects and enhancing pharmacological efficacy of drugs.

pH-dependent Gelation of Gastric Mucin

2005 ◽  
Vol 897 ◽  
Author(s):  
Rama Bansil ◽  
Jonathan Celli ◽  
Benard Chasan ◽  
Shyamsundar Erramilli ◽  
Zhenning Hong ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Low Ph ◽  
Gastric Mucin ◽  
Force Microscopy ◽  
Atomic Force ◽  
Visual Evidence ◽  
Ph Dependent ◽  
Microscopic Dynamic

AbstractWe discuss the mechanism by which gastric mucin forms a gel at low pH, which serves to protect the stomach from being damaged by the acidic gastric juice that it secretes. Frequency dependence of viscoelastic moduli of pig gastric mucin gels obtained by microscopic dynamic light scattering is presented. Atomic Force Microscopy provides direct visual evidence to indicate that mucin broken into its subunits does not gel at low pH.

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