scholarly journals Standardisation of an Agroinfiltration Protocol for Eggplant Fruits and Proving its Usefulness by Over-expressing the SmHQT Gene

2019 ◽  
Author(s):  
Prashant Kaushik
Keyword(s):  
Metabolic Engineering ◽  
Chlorogenic Acid ◽  
Transient Expression ◽  
Acid Content ◽  
Plant Transformation ◽  
Transformation Vector ◽  
Gene Construct ◽  
Native Promoter ◽  
Ideal System ◽  
Plant Transformation Vector

Eggplant is a fruit vegetable of family Solanaceae, and eggplant fruits are of different shape and sizes that render them as an ideal system for metabolic engineering. Here, we have developed an agroinfiltration protocol for the transient expression of a gene in the eggplant fruit using GUS bearing; pCAMBIA1304 vector. Thereafter, to prove the effectiveness of the developed protocol, we have used the eggplant hydroxycinnamoyl CoA-quinate transferase (SmHQT), which is the central enzyme studied to increase the chlorogenic acid content, in a gene construct with the specific promoter in a plant transformation vector (pBIN19). Also, in our cassette, we also co-expressed the P19 protein of Tomato bushy stunt virus (native promoter) to overexpress the protein. Overall, using the protocol, the chlorogenic content was increased by more than two folds in the transgenic tissues.

scholarly journals Gene Stacking for Fungal Resistance in Plant Transformation Vector

2017 ◽  
Vol 14 (3) ◽  
pp. 1211-1219
Author(s):  
Sonia Sharma ◽  
Gurtej Singh ◽  
Sadiq Pasha Shaik ◽  
Gagandeep Singh ◽  
Sumangala Bhat ◽  
...  
Keyword(s):  
Plant Transformation ◽  
Fruit Production ◽  
Fungal Diseases ◽  
Fungal Resistance ◽  
Hindiii Site ◽  
Transformation Vector ◽  
Fungal Disease Resistance ◽  
Genes Encoding ◽  
Production Form ◽  
Plant Transformation Vector

ABSTRACT: Fungal diseases like early blight, late blight, fusarium wilt cause 30-40 per cent loss in fruit production. Form past decade many transgenic plants had been developed using genes encoding chitinases and glucanases with the objective of imparting fungal disease resistance. Since the genes encoding chitinase and glucanase act synergistically. The study was performed to construct plant transformation vector pRAGS carrying both ech42 and bgn under single T-DNA region. Initially, HindIII site at 5' end of earlier cloned bgn (T. harzianum) was removed using primers during reamplification of the gene. The amplicons were cloned into pTZ57R/T containing T overhangs at Eco321 site and transferred to E. coli DH5a and further to plant transformation vector pBI121 which was named as pRA121. In order to clone another gene (ech42) into pRA121, expression cassette from iHP vector was transferred to pRA121 and named as pRAG121. Further in order to gain XhoI site for cloning ech42 gene into pRAG121, ech42 (pSUM1) was cloned into pYES2/CT, named as pSAG1, ech42 from pSAG1 cloned with KpnI and XhoI in pRAG121 and named as pRAGS121. The vector constructed in the present study can be used to transform important crop plants to have enhanced resistance to fungal diseases.

Construction of a new type of multi-gene plant transformation vector and genetic transformation of tobacco

2017 ◽  
Vol 61 (1) ◽  
pp. 13-23 ◽  
Author(s):  
Y. Dong ◽  
Y. C. Ren ◽  
M. S. Yang ◽  
J. Zhang ◽  
T. Qiu ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Plant Transformation ◽  
Transformation Vector ◽  
New Type ◽  
Plant Transformation Vector

The 35S promoter used in a selectable marker gene of a plant transformation vector affects the expression of the transgene

Planta ◽  
2005 ◽  
Vol 221 (4) ◽  
pp. 523-530 ◽  
Author(s):  
So Yeon Yoo ◽  
Kirsten Bomblies ◽  
Seung Kwan Yoo ◽  
Jung Won Yang ◽  
Mi Suk Choi ◽  
...  
Keyword(s):  
Plant Transformation ◽  
Selectable Marker ◽  
Marker Gene ◽  
35S Promoter ◽  
Selectable Marker Gene ◽  
Transformation Vector ◽  
Plant Transformation Vector

pBINPLUS: An improved plant transformation vector based on pBIN19

1995 ◽  
Vol 4 (4) ◽  
pp. 288-290 ◽  
Author(s):  
Fred A. van Engelen ◽  
Jos W. Molthoff ◽  
Anthony J. Conner ◽  
Jan-Peter Nap ◽  
Andy Pereira ◽  
...  
Keyword(s):  
Plant Transformation ◽  
Transformation Vector ◽  
Plant Transformation Vector

scholarly journals A Human Anti-Pseudomonas aeruginosa Serotype O6ad Immunoglobulin G1 Expressed in Transgenic Tobacco Is Capable of Recruiting Immune System Effector Function In Vitro

2007 ◽  
Vol 51 (9) ◽  
pp. 3322-3328 ◽  
Author(s):  
Michael D. McLean ◽  
Kurt C. Almquist ◽  
Yongfing Niu ◽  
Rhonda Kimmel ◽  
Zengzu Lai ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Immune System ◽  
Transgenic Tobacco ◽  
Plant Transformation ◽  
Bacterial Pathogen ◽  
Effector Function ◽  
Transformation Vector ◽  
Plant Transformation Vector ◽  
Human Igg1

ABSTRACT The production of a recombinant human IgG1 in transgenic tobacco was examined to determine whether a plant-derived antibody could recruit immune system effector function against a bacterial pathogen. A plant transformation vector was engineered to contain genes for a human kappa light chain and a human gamma-1 heavy chain with VH and VL sequences from a previously identified human IgG2 monoclonal antibody (MAb) that specifically binds to and opsonizes Pseudomonas aeruginosa serotype O6ad. Unique NcoI and NotI restriction sites were incorporated to flank these variable sequences, resulting in a plant transformation vector that could be engineered for expression of any other human IgG1 antibody, requiring only the substitution of other VH and VL antigen-binding coding sequences. The plant-produced IgG1 was determined to have high-mannose glycan content and to be capable of mediating opsonophagocytosis of P. aeruginosa serotype O6ad in vitro using human complement and human polymorphonuclear leukocytes. Thus, MAbs produced in plants from this vector could provide human IgG1 MAbs for targeting other pathogens that require the recruitment of immune system effector functions.

scholarly journals Transformation of tobacco plants with virEl gene derived from Agrobacterium tumefaciens pTiA6 and its effect on crown gall tumor formation

2001 ◽  
Vol 7 (3-4) ◽  
Author(s):  
E. Szegedi ◽  
A. Oberschall ◽  
S. Bottka ◽  
R. Oláh ◽  
D. Tinland
Keyword(s):  
Crown Gall ◽  
Plant Transformation ◽  
Northern Blot Analysis ◽  
Tumor Formation ◽  
Pcr Analysis ◽  
Wild Type ◽  
Crown Gall Tumor ◽  
Transformation Vector ◽  
Plant Transformation Vector ◽  
Foreign Dna

The VirEl protein plays a key role in the transport of VirE2 protein from the bacterium to the plant cell during crown gall tumor induction by Agrobacterium. The virEl gene of A. tutnefaciens pTiA6 was cloned into the plant transformation vector pTd33 yielding pTd93virEl that was introduced into A. tuniefaciens EHA101 and used for tobacco transformation. The presence of the foreign DNA in the putative transgenic plants was confirmed by PCR analysis. Nine of the 41 transformed plants formed only small tumors following infection with the wild-type A. vitis octopine strain AB3. This property was inherited into the T1 generation. The expression of virEl gene in TI plants was demonstrated by Northern blot analysis.  

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