The aim of this study was to examine the chemical composition and correlation between two types of date palm seeds (Phoenix dactylifera L.) grown in the north (Al-Bakrari) and south (Tafsert) of Libya. The chemical composition of two local date seeds was measured biochemical parameters on commonly used. Results showed that protein and lipid contents (Based on dry matter) ranged between 3.25 and 3.50 g/100 g ` and between 4.81 and 6.64 g/100 g, respectively. Carbohydrates content was about 80.74 and 82.48 g/100g for both samples. The ash content was ranged (1.85 - 2.30 g/100 g dry matter, while the moisture content did not exceed 7.6 % in any trial samples. The statistical analysis showed that lipid content had a significant positive correlation with moisture, (R2 = 0.751; p = 0.05). Further analysis indicated that both moisture and lipid had a highly significant negative correlation with ash (R2 = - 0.902 and - 0.901; p = 0.05) respectively, while ash having a highly significant positive correlation with carbohydrate (R2 = 0.967; p = 0.05). Based on the outcome of the study, it appears that the chemical composition of date seeds, it is recommended to use these residues in animals feed to obtain the highest productivity economically and utilization of the wasted resources on the other hand.