DETEKSI CMV DAN PYMoV PADA BENIH LADA (Piper nigrum) DENGAN TEKNIK ELISA

Salah satu kendala penting pada budidaya tanaman lada yaitu penyakit kerdil yang disebabkan oleh Piper yellow mottle virus (PYMoV) dan Cucumber mosaic virus (CMV). Tindakan pencegahan penyebaran penyakit dapat dilakukan salah satunya dengan penggunaan benih sehat bebas dari infeksi PYMoV dan CMV. Tujuan penelitian adalah mendeteksi keberadaan PYMoV dan CMV pada benih lada siap tanam secara serologi menggunakan teknik ELISA. Benih lada yang dideteksi berumur 5 bulan, berasal dari penangkar benih di Sukabumi (Jawa Barat) dan Purbalingga (Jawa Tengah). Varietas lada yang diambil di Sukabumi adalah Natar 1, Petaling, dan Lampung Daun Kecil., masing-masing sebanyak 10 sampel, sedangkan di Purbalingga hanya ada Natar 1, diambil 30 sampel. Di setiap lokasi pembibitan juga dilakukan pengamatan terhadap gejala infeksi virus yang ditemukan. Deteksi virus dilakukan secara Double Antibody Sandwich (DAS)-ELISA menggunakan antiserum Banana streak virus (BSV) untuk PYMoV dan antiserum CMV untuk deteksi CMV. Hasil ELISA dibaca nilai absorbannya dengan elisa reader pada panjang gelombang 405 nm. Sampel dinilai positif, jika nilai absorbansinya 1,5 kali lebih besar daripada kontrol negatif. Gejala infeksi virus pada benih bervariasi, yaitu klorotik, belang, dan belang disertai perubahan bentuk daun. Hasil pengujian ELISA menunjukkan bahwa 66% benih lada dari Sukabumi dan 46% dari Purbalingga terinfeksi virus CMV. Namun, tidak ada satu pun yang menunjukkan reaksi positif terhadap antiserum PYMoV. Hal ini diduga karena konsentrasi PYMoV terlalu rendah sehingga tidak terdeteksi secara ELISA. Untuk mencegah penyebaran virus, deteksi virus pada benih lada penting dilakukan sebelum digunakan sebagai bahan tanaman.

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Identification and transmission of Piper yellow mottle virus and Cucumber mosaic virus infecting black pepper (Piper nigrum ) in Sri Lanka

Plant Pathology ◽

10.1046/j.0032-0862.2002.00757.x ◽

2002 ◽

Vol 51 (5) ◽

pp. 537-545 ◽

Cited By ~ 29

Author(s):

D. P. P. De Silva ◽

P. Jones ◽

M. W. Shaw

Keyword(s):

Sri Lanka ◽

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Black Pepper ◽

Mottle Virus ◽

Piper Nigrum ◽

Yellow Mottle

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DETEKSI VIRUS PADA BIBIT LADA (Piper nigrum) DENGAN TEKNIK ELISA

Buletin Penelitian Tanaman Rempah dan Obat ◽

10.21082/bullittro.v27n2.2016.157-164 ◽

2017 ◽

Vol 27 (2) ◽

pp. 157 ◽

Cited By ~ 1

Author(s):

Maya Mariana ◽

NFN Miftakhurohmah

Keyword(s):

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Mottle Virus ◽

Piper Nigrum ◽

Yellow Mottle

Salah satu penyakit penting pada tanaman lada yaitu penyakit kerdil yang disebabkan oleh Piper yellow mottle virus (PYMoV) dan Cucumber mosaic virus (CMV). Tindakan pencegahan penyebaran penyakit ini dapat dilakukan dengan penggunaan bibit sehat. Deteksi dini PYMoV dan CMV pada bibit lada merupakan salah satu cara untuk mendapatkan bibit sehat, yang dapat dilakukan secara serologi menggunakan teknik ELISA. Tujuan penelitian adalah mendeteksi keberadaan virus pada bibit lada siap tanam pada pembibitan di Kabupaten Sukabumi dan Purbalingga. Sampel daun yang diuji diambil dari bibit lada berumur kurang lebih 5 bulan. Varietas yang diamati adalah Natar 1, Petaling, dan Lampung Daun Kecil. Hasil pengujian menunjukkan bahwa bibit lada terinfeksi virus di kedua lokasi pembibitan.

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Rapid detection of Piper yellow mottle virus and Cucumber mosaic virus infecting black pepper (Piper nigrum) by loop-mediated isothermal amplification (LAMP)

Journal of Virological Methods ◽

10.1016/j.jviromet.2013.06.012 ◽

2013 ◽

Vol 193 (1) ◽

pp. 190-196 ◽

Cited By ~ 34

Author(s):

A.I. Bhat ◽

A. Siljo ◽

K.P. Deeshma

Keyword(s):

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Rapid Detection ◽

Black Pepper ◽

Isothermal Amplification ◽

Mottle Virus ◽

Piper Nigrum ◽

Loop Mediated Isothermal Amplification ◽

Yellow Mottle

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KETAHANAN BEBERAPA KULTIVAR CABAI TERHADAP CUCUMBER MOSAIC VIRUS DAN CHILLI VEINAL MOTTLE VIRUS

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/j.hptt.27130-139 ◽

2012 ◽

Vol 7 (2) ◽

pp. 130-139

Author(s):

Muhammad Taufik ◽

Sri Hendrastuti Hidayat ◽

Sriani Sujiprihati ◽

Gede Suastika ◽

Sientje Mandang Sumaraw

Keyword(s):

Virus Infection ◽

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Yield Loss ◽

Disease Incidence ◽

Growth And Yield ◽

Mottle Virus ◽

Tolerance Response ◽

Chilli Veinal Mottle Virus ◽

Das Elisa

Resistance Evaluation of Chillipepper Cultivars for Cucumber Mosaic Virus and Chilli Veinal Mottle Virus. The use of resistance culivars is an important strategy for management of virus infection in chillipepper. A research was undergone to study the effect of single and mix infection of CMV and ChiVMV on the disease incidence and on the growth and yield of nine chillipepper cultivars, i.e. Cilibangi 4, Cilibangi 5, Cilibangi 6, Helem, Jatilaba, Tit Bulat, Tit Segitiga, Tit Super and Tampar. Mechanical inoculation was conducted to transmit the virus. Infection of the virus was then confirmed with DAS-ELISA. In general, inoculated chillipepper cultivars developed similar symptoms, i.e. mosaic type for CMV and mottle type for ChiVMV. More severe symptom was not always observed from mix infection of CMV and ChiVMV. Disease incidence occurred in the range of 16.67 – 86.0% and this caused 18.3 – 98.6% yield loss. Based on symptom expression, ELISA result, and reduction on yield, it can be concluded that all chillipepper cultivars used in this study could not hold up the virus infection. However, several cultivars showed tolerance response : Jatilaba, Tit Super, and Tampar for CMV; Cilibangi 4 for ChiVMV; Tit Super for mix infection; and Cilibangi 5 for CMV, ChiVMV, and mix infection. Further evaluation and investigation involving different chillipepper cultivars should be conducted.

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PCR multiplex para a detecção de BSV e CMV em bananeiras micropropagadas

Summa Phytopathologica ◽

10.1590/s0100-54052007000300003 ◽

2007 ◽

Vol 33 (3) ◽

pp. 229-232 ◽

Cited By ~ 2

Author(s):

Daniel Vasquez Figueiredo ◽

Paulo Sergio Torres Brioso

Keyword(s):

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Streak Virus ◽

Banana Streak Virus ◽

Pcr Multiplex

Um protocolo de PCR multiplex foi estabelecido para a detecção do Banana streak virus (BSV) e do Cucumber mosaic virus (CMV) em bananeiras micropropagadas. Estes vírus são responsáveis por perdas na produção de bananas em todo o mundo. Alguns trabalhos descrevem a integração do BSV no genoma B da bananeira. Contudo, a existência de bananeiras híbridas livres do BSV tem sido demonstrada. Ademais, determinadas estirpes do CMV não são transmitidas mecanicamente sob condições de laboratório, nem tampouco detectadas por testes sorológicos. Como conseqüência, a indexação de matrizes para cultura de tecido algumas vezes se mostra ineficiente. A metodologia apresentada neste trabalho sobrepõe esta dificuldade, pois se baseia na detecção do ácido nucléico viral presente em amostras foliares de bananeira. Na reação, foram usados os oligonucleotídeos BADNA 1A e BADNA 4, para a detecção do BSV, e "CMV senso" e "CMV antisenso" para a detecção do CMV. Após a eletroforese foi verificada a presença de dois fragmentos de DNA amplificados simultaneamente, um dos quais com 597 pb correspondente ao BSV e o outro, com 488 pb, correspondente ao CMV. Este resultado indica que o PCR multiplex pode ser utilizado como uma ferramenta adicional na indexação do BSV e do CMV em bananeiras propagadas por cultura de tecido.

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Incidencia y sintomatología de cinco virus en parcelas comerciales de chile seco en Aguascalientes, San Luis Potosí y Zacatecas, México

Revista Mexicana de Ciencias Agrícolas ◽

10.29312/remexca.v3i2.1471 ◽

2018 ◽

Vol 3 (2) ◽

pp. 381-390

Author(s):

Rodolfo Velásquez-Valle ◽

Luis Roberto Reveles-Torres ◽

Jaime Mena-Covarrubias

Keyword(s):

Tobacco Mosaic Virus ◽

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Potato Virus ◽

Potato Virus Y ◽

Tobacco Etch Virus ◽

Mottle Virus ◽

Pepper Mottle Virus ◽

San Luis ◽

Das Elisa

A nivel mundial el cultivo de chile es afectado por más de 60 enfermedades virales; sin embargo, poco se conoce acerca de ellas en el área productora de chile seco del norte centro de México por lo que el objetivo del presente trabajo consistió en detectar la presencia y sintomatología de cinco virus en parcelas comerciales de chile seco en los estados mencionados. Plantas de chile de los tipos mirasol y ancho fueron muestreadas y se anotó la presencia de síntomas como enanismo, clorosis, deformación de hojas, defoliación, necrosis vascular y ramas unidas. Las muestras fueron analizadas mediante la técnica DAS- ELISA empleando los antisueros para el virus del mosaico del tabaco (Tobacco mosaic virus: TMV), mosaico del pepino (Cucumber mosaic virus: CMV), Y de la papa (Potato virus Y: PVY), moteado del chile (Pepper mottle virus: PepMoV) y jaspeado del tabaco (Tobacco etch virus: TEV). Esos virus fueron identificados en plantas de chile colectadas en las parcelas comerciales de chile seco de los tres estados antes mencionados.

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Cucumber Mosaic Virus and Desmodium Yellow Mottle Virus Infections in Wild Groundnut (Apios americana)

Plant Disease ◽

10.1094/pd-74-0151 ◽

1990 ◽

Vol 74 (2) ◽

pp. 151 ◽

Cited By ~ 112

Author(s):

R. A. Valverde

Keyword(s):

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Mottle Virus ◽

Virus Infections ◽

Apios Americana ◽

Yellow Mottle

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First Report of Cucumber mosaic virus in Banana from Iran

Plant Disease ◽

10.1094/pd-89-0914a ◽

2005 ◽

Vol 89 (8) ◽

pp. 914-914 ◽

Cited By ~ 2

Author(s):

T. Ghotbi ◽

K. Bananej

Keyword(s):

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Plant Viruses ◽

Economic Losses ◽

Enzyme Linked Immunosorbent Assay ◽

Streak Virus ◽

Serological Tests ◽

Tropical Fruit ◽

Test Plants ◽

Das Elisa

Banana bunchy top virus (BBTV), Banana streak virus (BSV), and Cucumber mosaic virus (CMV) (genus Cucumovirus, family Bromoviridae [2]) cause widespread economic losses on banana (Musa sp.) throughout the world and have been reported on banana in different countries including Pakistan along its southeastern border with Iran (1). A survey was conducted from 2004–2005 to identify viruses infecting banana in greenhouses in different growing areas in northern Iran, Mazandaran Province (Sari, Babol, Behshahr, and Ghaemshahr cities). A total of 180 samples from seven banana-growing greenhouses with symptoms of mosaic, chlorosis, stunting, and fruit malformation were collected. All samples were tested for CMV with polyclonal antibodies using double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) (CMV strain D subgroup I; gifted by H. Lecoq, INRA, Avignion, France). For sap inoculation onto indicator test plants, selected ELISA-positive leaf samples were ground in chilled 0.01 M phosphate buffer, pH 7.0, containing 0.15% 2-mercaptoethanol. Chlorotic and necrotic local lesions developed on Chenopodium amaranticolor and Vigna unguiculata (cv. Mashad local) 10 and 12 days postinoculation, respectively. Cucumis sativus and Nicotiana rustica also developed systemic mosaic symptoms (3). All indicator test plants were rechecked for the presence of CMV using DAS-ELISA. On the basis of serological tests and indicator host plants reactions, CMV was identified in 32% of samples including Sari (13.8%), Babol (2.7%), Behshahr (10%) and Ghahemshahr (5%), respectively. Fifty-five samples did not react with CMV antiserum but the presence of symptoms resembling BBTV and BSV (4) emphasizes the need for further investigations to confirm the presence and identities of other viruses. References: (1) J. Bird and F. L. Wellman. Phytopathology 52:286, 1962. (2) S. K. Choi et al. J. Virol. Methods 83:67, 1999. (3) A. J. Gibbs and B. D. Harrison. Descriptions of Plant Viruses. No.1. CMI/AAB, Surrey, England, 1970. (4) R. C. Ploetz et al., eds. Compendium of Tropical Fruit Diseases. The American Phytopathological Society, St. Paul, MN, 1994.

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Reliable Detection for Bean yellow mosaic virus, Canna yellow streak virus, and Canna yellow mottle virus in Canna Varieties with Red Foliage

Plant Disease ◽

10.1094/pdis-05-14-0538-re ◽

2015 ◽

Vol 99 (2) ◽

pp. 188-194 ◽

Cited By ~ 4

Author(s):

Ravendra P. Chauhan ◽

Hayden F. Hamon ◽

Punsasi Rajakaruna ◽

Mark A. Webb ◽

Mark Payton ◽

...

Keyword(s):

Virus Infection ◽

Mosaic Virus ◽

Visual Assessment ◽

Mottle Virus ◽

Yellow Mosaic Virus ◽

Streak Virus ◽

Rt Pcr ◽

Disease Characteristics ◽

Yellow Mottle ◽

Canna Yellow Streak Virus

Cannas grow from rhizomes to produce colorful foliage that ranges from deep burgundy, bronze, green, purple veined, and variegated. Bean yellow mosaic virus (BYMV), Canna yellow streak virus (CaYSV), and Canna yellow mottle virus (CaYMV) are problematic viruses infecting cannas. Their disease characteristics have been reported in green-leaved varieties. This study investigated if rhizome planting stocks can be a source of virus infection. PCR and RT-PCR tests identified BYMV, CaYSV, and CaYMV sequences in 20 canna rhizomes and newly emerging leaves. Immunosorbent electron microscopy tests identified filamentous potyvirus particles in rhizome and leaf tissue. In addition, disease characteristics were examined in a subset of red-leaved varieties ‘Australia’, ‘Burning Ember’, and ‘Red Futurity’ planted in pots in the greenhouse. Plants were assigned identifying codes, visual disease ratings, and samples were taken for RT-PCR and PCR virus detection assays. Statistical analysis was carried out to compare disease ratings with RT-PCR and PCR test results. Visual assessment was found to be not a reliable indicator of virus infection in ‘Australia’ and ‘Burning Ember’ plants. ‘Red Futurity’ produced the most obvious pattern of mosaic disease and virus symptoms were easier to identify in this variety. This study demonstrated that visual assessment was an ineffective method for disease identification for the red-leaved varieties. Growers would be well advised to utilize molecular testing to identify infected plants to aid in the clean-up of the crop.

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