Immunomodulation Effect of Kaempferia galanga Ethanolic Extract On In Vitro Lymphocyte Cell Proliferation

<p><em>Kaempferia galanga</em> L. is a traditional medicine with antitumor properties, as indicated by its immunomodulatory activities. This study aimed to determine the effect of <em>K. galanga</em> on lymphocyte cell proliferation activity as an indicator of immunomodulatory properties. This study was conducted at the Indonesian Research Center for Veterinary Science (February to April 2018). The immunomodulatory activity of the extract was evaluated with an <em>in vitro</em> splenocyte proliferation assay. The assay was based on cellular enzymatic synthesis to transform the XTT from formazan tetrazolium as an indicator. The <em>K. galanga</em> extract was obtained by 96% ethanol extraction. The test was conducted in an aseptic condition, consisted of five treatment groups with three replications each. Three groups of splenocyte cell culture, each with extract concentration of 2.5 µg.ml^-1, 25 µg.ml^-1, and 250 µg.ml^-1, as well as a positive (Concanavalin A/Con A) and negative (cell only) control. The cell suspension (10x10⁴cells/ml) was distributed on 96-well plates and cultured following the treatment groups. The same five plates were made for five days of observation and retrieved daily by observing an Elisa reader at 450 nm. The extract of <em>K. galanga </em>at 2.5 µg.ml^-1, 25 µg.ml^-1, and 250 µg.ml^-1 significantly (P &lt;0.05) promoted splenocyte proliferation compared to control. Therefore, it was expected that <em>K. galanga </em>has a high potential to be used as immunomodulators. Hence, further investigations should be done to clarify the mechanisms of the immunomodulatory effect of <em>K. galanga</em> as an antitumor <em>in vivo</em>.</p>

PENGARUH KOMBINASI PUPUK ORGANIK DAN PUPUK HAYATI TERHADAP PERTUMBUHAN, PRODUKSI DAN MUTU PEGAGAN

<em>Gotu kola is one of the medicinal plants that has begun to be cultivated by the Indonesian people. It’s cultivation needs to follow the GAP to produce the qualified yield, including the use of fertilizers. Biofertilizers have been widely developed to reduce adverse effects of chemical fertilizers to the environment, thus an organic cultivation method can be an alternative. The aim of this study was to determine the effect of biofertilizer on growth and yield of gotu kola. This research was an experimental study with a completely randomized design (CRD) using 8</em> <em>planting media treatments consisting of (a) Soil + manure + dolomite; (b) Soil manure + 1 g biofertilizer; (c) Soil + dolomite + 1g biofertilizer; (d) Soil + manure + 3 biofertilizer; (e) Soil + dolomite + 3 biofertilizer; (f) Soil + manure + 5 g biofertilizer; (g) Soil + dolomite + 5 g biofertilizer; and (h) Soil + 3 g</em> <em>biofertilizer. Parameters observed were leaf area, stem length, number of leaves, number of tillers, fresh weight, dry weight and asiaticoside content. The results showed that combination treatment of biofertilizer (1 g) with dolomite (30 g) gives better effect in increasing the number of tillers and number of leaves, while the combination of biofertilizer (5 g) and dolomite (30 g) is the best for asiaticoside levels in gotu cola.</em>

FARMING PRODUCTION COSTS AND RELATIVE COMPETITIVENESS OF INDONESIAN BLACK AND WHITE PEPPERS

<p>Indonesia is one of the major pepper (Piper nigrum L.) producer countries in the world. The main pepper products are distinguished into black and white pepper. Each region has a tradition to produce each of the products and influencing cultivation practices and cost structure. This research was aimed to analyze the cost of productions of black and white pepper and their relative competitiveness to the pepper price at the farm level with conventional and improved cultivation practices. The survey methods were used to obtain the primary data from respondents selected with the snowball sampling method. Lampung and Bangka Belitung Islands were chosen to represent the black and white peppers of smallholders, respectively. The result showed that the farms with conventional cultivation practices did not have sustainable relative competitiveness, indicating higher production costs than the lowest prices received in the long term. On the other hand, relative competitiveness was relatively better in farms that implemented improved cultivation practices. Therefore, to achieve sustainable relative competitiveness, pepper farms should apply improved cultivation practices. The relative competitiveness of white pepper was better than black pepper because the productivity of white pepper was higher even though the production cost was also a little bit higher than black pepper.</p>

ISOLASI DAN IDENTIFIKASI SENYAWA ANTIOKSIDAN 2-ETILHEKSIL-4-METOKSISINAMAT DARI EKSTRAK BIJI ALPUKAT (Persea americana Mill.)

<em><span lang="EN-US">Avocado (</span></em><span lang="EN-US">Persea americana</span><em><span lang="EN-US"> Mill.) is a plant widely found in tropical regions such as Indonesia. Currently, people only consume the avocado flesh and dispose of the seed. This study aimed to examine the antioxidant activity, isolate and identify the active antioxidant structure from avocado seed extracts. The trial was conducted from January to June 2021 at the Chemical Laboratory of Natural Substance, Research Center for Biotechnology, LIPI. The avocado fruit used in this research was obtained from Cibitung Central Market, Bekasi. The solvents used in the extraction were n-hexane, ethyl acetate, 96% ethanol, and water with multilevel extraction. Antioxidant activity was tested using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical method. The structure of the active compound was determined using UV-Vis spectrophotometry, Fourier Transformation Infrared Spectroscopy (FTIR), and GCMS. The 96% ethanol extract had the highest antioxidant activity with EtOH.4.3.1 isolate as the most active antioxidant isolate with IC50 23,07±1,63 μg.ml-1. The identification results of isolate EtOH.4.3.1 of avocado seed extract with UV-Vis spectrophotometry, FTIR, and GCMS indicated 2-Ethylhexyl-4-methoxycinnamate acting as an antioxidant. Further analysis with Nuclear Magnetic Resonance (NMR) spectrometry of protons and carbon is required to determine the number and position of protons and carbon in the chemical structure.</span></em>

The Compatibility of Piper retrofractum Vahl. and Curcuma xanthorrhiza Roxb. Extracts for Controlling Helopeltis antonii Sign.

<p><em>Plant extracts as a botanical insecticide can be used in either single or mixture forms. Mixed extracts have advantages: reducing dependence on one plant species, being the synergistic effect which can increase the effectiveness of formulas, and reducing the possibility of resistance emergence. This study aimed to obtain compatible and synergistic comparisons of </em>P. retrofractum<em> with </em>C. xanthorrhiza<em> extracts that effectively control the third instar nymph of </em>Helopeltis antonii<em>. The comparison of </em>P. retrofractum<em> and </em>C. xanthorrhiza<em> extracts were 4:1, 2:1, 1:1, 1:2, and 1:4 (w/w). The experiment was performed in a completely randomized design using the contact method. The LC₅₀, LC₉₀, and LC₉₅ values were calculated using the PoloPlus Ver 1.0 Program. The mixture interaction was determined from the Combination Index value. The results showed that</em> P. retrofractum<em> extract was compatible and synergistic with </em>C. xanthorrhiza<em> extract at a ratio of 2:1 (w/w). These were the best combination shown by the lowest LC₅₀, LC₉₀, and LC₉₅ values and showed consistently strong synergistic properties compared to other concentrations. The components of</em><em> </em><em>P. retrofractum</em><em> </em><em>may be responsible for the insecticidal properties are piperine; piperanine; piperidine; 3,4-Pyridinedimethanol,6-methyl; Piperidine,1-[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]-,(Z,Z)-; and (2E,4E,12E)-1-(Piperidine-1-yl)octadeca-2,4,12—trien-1-one. The insecticidal activity of </em>C. xanthorrhiza<em> may associated with its content of</em><em> xanthorrhizol, Ar-curcumene, β-curcumene, curzerene, germacrone</em>, <em>7-zingiberene, caryophyllene, and β-bisabolol. A combination of these two plant extracts has the potential to be further developed as a botanical insecticide to control</em> H. antonii<em>.</em></p>

KERAGAMAN GENETIK 64 AKSESI KUNYIT ASAL INDONESIA BERDASARKAN MARKA P450-BASED ANALOGUE (PBA)

<em>Turmeric is a rhizome producing plant with many utilization such as for consumption, medicine, and colorant industries. The development of superior turmeric varieties in Indonesia needs to be supported by genetic diversity information availability. Despite its potential, genetic diversity information of Indonesian turmeric has not been widely observed. A molecular marker is used to address genetic diversity information with the accurate result due to minimum environmental influences. PBA can detect the P450 gene as a functional marker, which is related to the synthesis of secondary metabolites in a wide genome area. Thus, it can be used as an alternative marker to identify genetic diversity. This research aimed to obtain genetic diversity information of 64 turmeric accessions using eight primer sets of P450-Based Analogue (PBA). The study was conducted in the Central Laboratory of Padjadjaran University from June 2019 to January 2020. Results showed that the full 133 bands were detected with a range of allele number 8 - 45 bands and an average of 22.3 bands per allele. PIC analysis showed six primer sets of PBA had high polymorphisms ranged from 0.90 to 0.98, hence categorized </em><em>PBA as a highly informative marker. Cluster analysis divided 64 turmeric accessions into two main clusters based on a similarity index ranged from 0.01 to 0.83. The accession of CL-GTL01 origins from Gorontalo had a low similarity coefficient of 0.01 to the other 64 accessions cluster. On the other hand, CL-NTB01 dan CL-PPB01 had the highest similarity index of 0.83. Based on the PIC value, the total number of polymorphic bands, and genetic distance, it can be concluded that local Indonesian turmeric had wide diversity based on PBA marker.</em>

AKTIVITAS METABOLIT SEKUNDER PEMACU PERTUMBUHAN DARI BAKTERI ENDOFIT ASAL KUNYIT PUTIH (Curcuma zedoaria ROSC)

<em><span>Endophytic bacteria colonize the plant tissue and contribute to the host plant's growth, development, and adaptation. This study aimed to examine the endophytic bacteria diversity associated with white turmeric (</span></em><span>Curcuma zedoaria</span><em><span>) and determine its activity as a plant growth promoter. White turmeric plants were obtained from the Indonesian Spices and Medicinal Crop Research Institute (Balittro). The isolation of endophytic bacteria from leaves, a primary and secondary rhizome of white turmeric using Nutrient Agar (NA) medium. Identification of the isolates was conducted based on 16S rDNA. The isolates were tested for their ability as a plant growth promoter and enzyme producer. Based on the morphological characteristic, 21 isolates were obtained from the primary rhizome (47.62%), secondary rhizome (19.05%), and leaves (33.33%), respectively. The sequencing result of 16S rDNA showed that the endophytic bacteria community consisted of four phyla, </span></em><span>β-proteobacteria</span><span>, γ-proteobacteria, Flavobacteria,<em> </em>Firmicutes,<em> which represented eight genera. Five isolates had several activities as a plant growth promoter, while six isolates had several enzymatic activities. Thirteen isolates had both activities, as a plant growth promoter and enzyme producer, while eight isolates only had single action. Endophytic bacteria potential as plant growth promoters can be used for supporting the cultivation of white turmeric plants.</em></span>

AKTIVITAS ANTIINFLAMASI EKSTRAK ETANOL DAUN KOPI ARABIKA (Coffea arabica L.) PADA EDEMA TIKUS

<em>The coffee leaf contains flavonoid compounds that are known as an antiinflammation agent. The study aimed to determine antiinflammatory activity of arabica coffee's leaf extract (</em>Coffea arabica<em> L.) against edema on white rat soles of carrageenan-induced rats. The research began with the maceration extraction of coffee leaf in 70% ethanol. The inflammation test was held by measuring the formation of edema using a plethysmometer. The dosages of arabica coffee leaf extract used were 60 mg.kg^-1, 120 mg.kg^-1, and 180 mg.kg^-1 rat weight of white Sprague Dawley rat (</em>Rattus norvegicus<em>). The data were analyzed by Kruskal Wallis and Mann Whitney non-parametric tests. Phytochemical screening showed that arabica coffee leaf extract contained flavonoid, saponin, alkaloid, tannin and triterpenoids compound. The arabica coffee leaf extract showed the best antiinflammatory activity at the dose of 180 mg.kg^-1 rat weight with 71.66% inhibition percentage at the 5^th hour, which was better than the positive control (66.91% edema inhibition at the same hour). This result determined that the leaf extract of arabica coffee had antiinflammatory activity. However, it is necessary to study the acute and chronic toxicity and formulates the arabica coffee leaf extract as a pharmacy product.</em>

POTENSI EKSTRAK DAUN PALIASA (Kleinhovia hospita) SEBAGAI ANTI Plasmodium falciparum

<em>Paliasa (</em>Kleinhovia hospita<em>) is known as a plant that has been used empirically for malaria treatment, especially in Eastern Indonesia. However, scientific publications regarding to the antiplasmodial activity of these natural resources are still limited. The aim of this study was to examine the potency of paliasa leaves as antiplasmodial against </em>Plasmodium falciparum<em> parasite. The procedure included sample and extract preparation, antiplasmodial in vitro activity testing on </em>P. falciparum<em> strain 3D7, and phytochemical screening using Thin Layer Chromatography (TLC). The extracts and fractions were prepared through maceration process for 72 hours with 96% ethanol, then continued with multilevel liquid-liquid partition using hexane, ethyl acetate and 96% methanol. Antiplasmodial </em>in vitro<em> testing showed that ethyl acetate (IC₅₀ </em><em>1.08</em> <em>µg.ml^-1</em><em>) and hexane (IC₅₀ 1.24 µg.ml^-1) fractions were include to the highly activity category. The research samples contained alkaloids, triterpenoids and steroids as the major compounds. The terpenoid alkaloid compound was a cycloartane triterpenoid alkaloid that had been isolated from paliasa leaves. Therefore, it is assumed that the leaf of palasia has a compound with antiplasmodial activity. However, more research needs to be done to determine the active compound and the antiplasmodial mechanisms involved</em>

Antioxidant and Anti-Elastase Activity of Ethanol Extract of Tomato (Solanum lycopersicum L.)

Skin aging due to the damage caused by ultraviolet radiation and toxic ingredients in cosmetics is still a problem. Tomato has antioxidant and skin protection activities. The study aimed to investigate the potential of tomato as an antioxidant and elastase inhibitor. A 170 g of tomato simplicial powder was extracted using ethanol 70% by the maceration method. Antioxidant activity was measured through 2,2’-Azino-bis 3-ethyl benzothiazoline-6-sulphonic acid (ABTS)-reducing activity. The antiaging activity was measured through anti-elastase activity. Tomato extract (SLE) showed strong ABTS-reducing activity (IC₅₀ = 86.66 ± 10.58) and very strong anti-elastase activity (IC₅₀= 19.73 ± 0.44). In conclusion, there was a linear correlation between antioxidant activity and anti-elastase activity. However, the antioxidant activity and anti-elastase activity of tomatoes were still below lycopene (IC₅₀ antioxidant = 49.23 ± 2.06 µg.ml^-1 and IC₅₀ anti-elastase = 10.39 ± 0.43 49.23 ± 2.06 µg.ml^-1). However, it was worth to be developed as a natural product as an antioxidant and anti-elastase. Further study is required to do fractionation to get the purer lycopene compound from tomato.