Acute toxicity of silver nitrate to in vitro fertilization of the sand dollar, Echinarachnius parma
Considered one of the most toxic heavy metals, interest in silver (both ionic and bound forms) has increased over the past few years due to the production of consumer goods containing Ag⁺-releasing nanoparticles. Investigation into acceptable environmental limits has generated a substantial amount of evidence that even at very low concentrations, silver exposure is detrimental to organism health. This study employed the echinoderm fertilization assay to evaluate acute silver toxicity to a marine invertebrate, Echinarachnius parma. Gametes were procured from E. parma and fertilization success under control conditions was compared to that at varying treatment concentrations of silver nitrate. Exposure to silver nitrate significantly decreased percent fertilization in all treatment concentrations. Remarkably, at concentrations as low as 10⁻⁹ M AgNO₃ percent fertilization decreased by 20-30% compared to the control. The results of this study are consistent with the existing literature, adding to the expanding collection of data that emphasizes the need for more stringent environmental silver regulation criteria in order to ensure the protection of aquatic ecosystems.