A Study on Prevalence of HPV Infection in Head and Neck Cancer

Background: Head and neck cancer (HNC) developed due to the number of risk factors, including infection of Human Papillomavirus (HPV). The genetic predisposition also plays an important role in deregulating the NF-κB pathway, and certain polymorphisms are reported to affect the pathway genes. Objectives: The present study was conducted for the detection of HPV and polymorphisms in the NF-κB1 gene of HNC patients in the Pakistani population. Methods: Genomic DNA from HNC tumors samples were extracted using the Exgene SV DNA extraction Kit. Allele-specific PCR and direct sequencing were done for analysis of NF-κB1 SNPs, 94ins/del (rs28362491), rs1598858, and rs4648068. Results: The genotypes AG (36.2%/ 12%) of rs1598858, and AG (28.3%/ 12%) and GG (28.3%/ 22%) of rs4648068 were associated with significantly (p≤0.05) increased risk of head and neck cancer in studied population. Furthermore, among the HNC cases, genotypes AGrs1598858 (p≤0.014) and GGrs4648068 (p≤0.001) had increased risk of HPV related cancers. Tobacco use (OR-3.158442; [1.140, 8.754]), lymph nodes involvement (OR 4.05128; [1.854, 8.852]), and poorly differentiated tumors (OR 1.997155; [0.940, 4.245]) were positively associated with HPV induced cancers. Conclusion: It was the first comprehensive study from Pakistan, to evaluate the polymorphic variants of NF-κB1. Genotypes AGrs4648068, GGrs4648068, and AGrs1598858 of NF-κB1 gene are associated with increased risk of head and neck cancers in the Pakistani population. It can be concluded that HPV infection, lymph nodes and tobacco use can act synergetic to each other and add up in modulating HNC when present together with intronic SNPs of NF-κB1 gene.

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Oral HPV infection and persistence in patients with head and neck cancer

Oral Surgery Oral Medicine Oral Pathology and Oral Radiology ◽

10.1016/j.oooo.2013.06.019 ◽

2013 ◽

Vol 116 (4) ◽

pp. 474-484 ◽

Cited By ~ 13

Author(s):

Patrizia Morbini ◽

Barbara Dal Bello ◽

Paola Alberizzi ◽

Laura Mannarini ◽

Niccolò Mevio ◽

...

Keyword(s):

Head And Neck Cancer ◽

Head And Neck ◽

Neck Cancer ◽

Hpv Infection ◽

Oral Hpv

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Alcohol and Tobacco Increases Risk of High Risk HPV Infection in Head and Neck Cancer Patients: Study from North-East Region of India

PLoS ONE ◽

10.1371/journal.pone.0140700 ◽

2015 ◽

Vol 10 (10) ◽

pp. e0140700 ◽

Cited By ~ 20

Author(s):

Rupesh Kumar ◽

Avdhesh Kumar Rai ◽

Debabrata Das ◽

Rajjyoti Das ◽

R. Suresh Kumar ◽

...

Keyword(s):

Head And Neck Cancer ◽

High Risk ◽

Head And Neck ◽

Cancer Patients ◽

Neck Cancer ◽

Hpv Infection ◽

North East ◽

East Region ◽

High Risk Hpv

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HPV Infection in Head and Neck Cancer

10.1007/978-3-319-43580-0 ◽

2017 ◽

Cited By ~ 3

Keyword(s):

Head And Neck Cancer ◽

Head And Neck ◽

Neck Cancer ◽

Hpv Infection

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A Five-MicroRNA Signature Predicts Survival and Disease Control of Patients with Head and Neck Cancer Negative for HPV Infection

Clinical Cancer Research ◽

10.1158/1078-0432.ccr-18-0776 ◽

2018 ◽

Vol 25 (5) ◽

pp. 1505-1516 ◽

Cited By ~ 18

Author(s):

Julia Hess ◽

Kristian Unger ◽

Cornelius Maihoefer ◽

Lars Schüttrumpf ◽

Ludmila Wintergerst ◽

...

Keyword(s):

Head And Neck Cancer ◽

Disease Control ◽

Head And Neck ◽

Neck Cancer ◽

Hpv Infection

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EGFR mutations and human papillomavirus in squamous cell carcinoma of tongue and tonsil

Journal of Clinical Oncology ◽

10.1200/jco.2006.24.18_suppl.10027 ◽

2006 ◽

Vol 24 (18_suppl) ◽

pp. 10027-10027 ◽

Cited By ~ 2

Author(s):

I. Na ◽

H. Kang ◽

Y. Park ◽

J. Koh ◽

S. Cho ◽

...

Keyword(s):

Head And Neck Cancer ◽

Human Papillomavirus ◽

Head And Neck ◽

Neck Cancer ◽

Egfr Mutation ◽

Tongue Cancer ◽

Disease Free Survival ◽

Hpv Infection ◽

Egfr Mutations ◽

Tonsil Cancer

10027 Background: Some studies reported the presence of somatic mutation of EGFR in head and neck cancer. Human papillomavirus (HPV) was associated with some types of head and neck cancer. We performed this study to determine clinical significance of EGFR mutation and its association with HPV infection in patients with tongue and tonsil cancer. Methods: We sequenced exons 18–21 of EGFR TK domain and HPV typing was performed using the HPV DNA chip in tissues of patients with tongue and tonsil cancer. Univariate and multivariate analysis was performed. Results: Eighty-one patients were included. We detected EGFR mutation in 10 (12%) patients. Mutation was significantly frequent in never-smoker (21% versus 5%; P = 0.031). The mutation frequency did not differ between tongue and tonsil cancer (12% vs. 13%, respectively; P = 0.978). There was no difference between female and male (12% vs. 14%, respectively; P = 0.754). Eleven of 81 (14%) patients were HPV positive. HPV positivity was more common in young (<60 years) than older (≥60 years) patients (20% vs. 3%, respectively; P = 0.039). Patients with tonsil cancer showed more frequent HPV positivity than tongue cancer (42% vs. 2%, respectively; P < 0.001). There was no association between HPV positivity and EGFR mutation. In terms of disease-free survival, patients with EGFR mutation had better survival independently of stage and primary site (hazard ratio, 0.246; 95% CI, 0.075 to 0.802). Conclusions: We did not find association between EGFR mutation and HPV positivity. Patients with EGFR mutation showed better outcome in tongue and tonsil cancer. These results suggest that EGFR mutation may be a prognostic factor in patients with head and neck cancer. No significant financial relationships to disclose.

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PO-128: HPV Infection in Head and Neck Cancer. Is P16 Immunohistochemistry a Reliable Surrogate Marker?

Radiotherapy and Oncology ◽

10.1016/s0167-8140(15)34747-2 ◽

2013 ◽

Vol 106 ◽

pp. S54-S55

Author(s):

F. Bussu ◽

C. Parrilla ◽

G. Delogu ◽

M. Sali ◽

N. Dinapoli ◽

...

Keyword(s):

Head And Neck Cancer ◽

Head And Neck ◽

Neck Cancer ◽

Surrogate Marker ◽

Hpv Infection ◽

P16 Immunohistochemistry

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Immunometabolic Alterations by HPV Infection: New Dimensions to Head and Neck Cancer Disparity

JNCI Journal of the National Cancer Institute ◽

10.1093/jnci/djy207 ◽

2019 ◽

Vol 111 (3) ◽

pp. 233-244 ◽

Cited By ~ 5

Author(s):

Sanjib Chaudhary ◽

Koelina Ganguly ◽

Sakthivel Muniyan ◽

Ramesh Pothuraju ◽

Zafar Sayed ◽

...

Keyword(s):

Head And Neck Cancer ◽

Head And Neck ◽

Neck Cancer ◽

Hpv Infection ◽

Cancer Disparity

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The impact of HPV infection on survival of patients with non-oropharyngeal head and neck cancer.

Journal of Clinical Oncology ◽

10.1200/jco.2017.35.15_suppl.6048 ◽

2017 ◽

Vol 35 (15_suppl) ◽

pp. 6048-6048 ◽

Cited By ~ 1

Author(s):

Samer Alsidawi ◽

Gustavo Figueiredo Marcondes Westin ◽

Ashish V. Chintakuntlawar ◽

Scott H. Okuno ◽

Katharine Andress Rowe Price

Keyword(s):

Multivariate Analysis ◽

Head And Neck Cancer ◽

Head And Neck ◽

Neck Cancer ◽

Tumor Grade ◽

Hpv Infection ◽

Smoking History ◽

Hpv Status ◽

Number Of Patients ◽

The Impact

6048 Background: The role of Human Papilloma Virus (HPV) infection in non-oropharyngeal squamous cell carcinoma (non-OPSCC) of the head and neck is unknown. Current available studies have yielded conflicting results due to limited number of patients. We present a large analysis from the National Cancer Database (NCDB) evaluating HPV-positive non-OPSCC. Methods: Using the NCDB registry, we included adults diagnosed with non-OPSCC from 2004-2012 with available HPV status. A cohort of patients with OPSCC was analyzed for HPV prevalence comparison. Survival analysis was performed using Kaplan-Meier method and stratified using HPV-status. The prognostic effect of variables was studied using Cox proportional hazards models. The JMP software was used for statistical analysis. Results: A total of 8726 non-OPSCC patients were identified and primary sites included the oral cavity (50%), larynx (41%) and hypopharynx (9%). 11% of non-OPSCC patients had evidence of infection with high-risk HPV strains compared to 61% of OPSCC patients. HPV-positive non-OPSCC patients presented at slightly younger age, had more advanced stage and higher tumor grade compared to HPV-negative patients (P < 0.01). HPV-positive non-OPSCC patients had better survival than HPV-negative patients (HR 0.82, 95% CI 0.72-0.93, P < 0.01) and this was most pronounced in patients with locally advanced disease (5-year survival 50% versus 40%, HR 0.69, 95% CI 0.6-0.8, P < 0.01). A univariate and multivariate analysis were performed adjusting for age, sex, race, stage, primary site, Charlson/Deyo comorbidity score, financial income, tumor grade, surgery, radiation and chemotherapy administration. Smoking history was unavailable. HPV positivity was an independent predictor of better survival in non-OPSCC in multivariate analysis (HR 0.69, 95% CI 0.59-0.8, P < 0.01). Conclusions: HPV infection is seen in a subset of patients with non-OPSCC head and neck cancer and these present with more advanced tumors. The survival of patients with HPV-positive non-OPSCC is significantly better than HPV-negative tumors. Routine HPV testing and enrollment in treatment de-intensification clinical trials similar to OPSCC might be appropriate for this patient population.

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