Oral HPV infection and persistence in patients with head and neck cancer

Background: Head and neck cancer (HNC) developed due to the number of risk factors, including infection of Human Papillomavirus (HPV). The genetic predisposition also plays an important role in deregulating the NF-κB pathway, and certain polymorphisms are reported to affect the pathway genes. Objectives: The present study was conducted for the detection of HPV and polymorphisms in the NF-κB1 gene of HNC patients in the Pakistani population. Methods: Genomic DNA from HNC tumors samples were extracted using the Exgene SV DNA extraction Kit. Allele-specific PCR and direct sequencing were done for analysis of NF-κB1 SNPs, 94ins/del (rs28362491), rs1598858, and rs4648068. Results: The genotypes AG (36.2%/ 12%) of rs1598858, and AG (28.3%/ 12%) and GG (28.3%/ 22%) of rs4648068 were associated with significantly (p≤0.05) increased risk of head and neck cancer in studied population. Furthermore, among the HNC cases, genotypes AGrs1598858 (p≤0.014) and GGrs4648068 (p≤0.001) had increased risk of HPV related cancers. Tobacco use (OR-3.158442; [1.140, 8.754]), lymph nodes involvement (OR 4.05128; [1.854, 8.852]), and poorly differentiated tumors (OR 1.997155; [0.940, 4.245]) were positively associated with HPV induced cancers. Conclusion: It was the first comprehensive study from Pakistan, to evaluate the polymorphic variants of NF-κB1. Genotypes AGrs4648068, GGrs4648068, and AGrs1598858 of NF-κB1 gene are associated with increased risk of head and neck cancers in the Pakistani population. It can be concluded that HPV infection, lymph nodes and tobacco use can act synergetic to each other and add up in modulating HNC when present together with intronic SNPs of NF-κB1 gene.

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A Study on Prevalence of HPV Infection in Head and Neck Cancer

Indian Journal of Cancer Education and Research ◽

10.21088/ijcer.2321.9815.5217.11 ◽

2017 ◽

Vol 5 (2) ◽

pp. 127-132

Author(s):

Palla Madhuri ◽

◽

C. Sanjeeva Kumari ◽

Keyword(s):

Head And Neck Cancer ◽

Head And Neck ◽

Neck Cancer ◽

Hpv Infection

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Alcohol and Tobacco Increases Risk of High Risk HPV Infection in Head and Neck Cancer Patients: Study from North-East Region of India

PLoS ONE ◽

10.1371/journal.pone.0140700 ◽

2015 ◽

Vol 10 (10) ◽

pp. e0140700 ◽

Cited By ~ 20

Author(s):

Rupesh Kumar ◽

Avdhesh Kumar Rai ◽

Debabrata Das ◽

Rajjyoti Das ◽

R. Suresh Kumar ◽

...

Keyword(s):

Head And Neck Cancer ◽

High Risk ◽

Head And Neck ◽

Cancer Patients ◽

Neck Cancer ◽

Hpv Infection ◽

North East ◽

East Region ◽

High Risk Hpv

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HPV Infection in Head and Neck Cancer

10.1007/978-3-319-43580-0 ◽

2017 ◽

Cited By ~ 3

Keyword(s):

Head And Neck Cancer ◽

Head And Neck ◽

Neck Cancer ◽

Hpv Infection

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A Five-MicroRNA Signature Predicts Survival and Disease Control of Patients with Head and Neck Cancer Negative for HPV Infection

Clinical Cancer Research ◽

10.1158/1078-0432.ccr-18-0776 ◽

2018 ◽

Vol 25 (5) ◽

pp. 1505-1516 ◽

Cited By ~ 18

Author(s):

Julia Hess ◽

Kristian Unger ◽

Cornelius Maihoefer ◽

Lars Schüttrumpf ◽

Ludmila Wintergerst ◽

...

Keyword(s):

Head And Neck Cancer ◽

Disease Control ◽

Head And Neck ◽

Neck Cancer ◽

Hpv Infection

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Sensitivity and specificity of oral HPV detection for HPV-positive head and neck cancer

Oral Oncology ◽

10.1016/j.oraloncology.2017.12.008 ◽

2018 ◽

Vol 77 ◽

pp. 52-56 ◽

Cited By ~ 19

Author(s):

Brooke J. Gipson ◽

Hilary A. Robbins ◽

Carole Fakhry ◽

Gypsyamber D'Souza

Keyword(s):

Head And Neck Cancer ◽

Head And Neck ◽

Sensitivity And Specificity ◽

Neck Cancer ◽

Oral Hpv

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EGFR mutations and human papillomavirus in squamous cell carcinoma of tongue and tonsil

Journal of Clinical Oncology ◽

10.1200/jco.2006.24.18_suppl.10027 ◽

2006 ◽

Vol 24 (18_suppl) ◽

pp. 10027-10027 ◽

Cited By ~ 2

Author(s):

I. Na ◽

H. Kang ◽

Y. Park ◽

J. Koh ◽

S. Cho ◽

...

Keyword(s):

Head And Neck Cancer ◽

Human Papillomavirus ◽

Head And Neck ◽

Neck Cancer ◽

Egfr Mutation ◽

Tongue Cancer ◽

Disease Free Survival ◽

Hpv Infection ◽

Egfr Mutations ◽

Tonsil Cancer

10027 Background: Some studies reported the presence of somatic mutation of EGFR in head and neck cancer. Human papillomavirus (HPV) was associated with some types of head and neck cancer. We performed this study to determine clinical significance of EGFR mutation and its association with HPV infection in patients with tongue and tonsil cancer. Methods: We sequenced exons 18–21 of EGFR TK domain and HPV typing was performed using the HPV DNA chip in tissues of patients with tongue and tonsil cancer. Univariate and multivariate analysis was performed. Results: Eighty-one patients were included. We detected EGFR mutation in 10 (12%) patients. Mutation was significantly frequent in never-smoker (21% versus 5%; P = 0.031). The mutation frequency did not differ between tongue and tonsil cancer (12% vs. 13%, respectively; P = 0.978). There was no difference between female and male (12% vs. 14%, respectively; P = 0.754). Eleven of 81 (14%) patients were HPV positive. HPV positivity was more common in young (<60 years) than older (≥60 years) patients (20% vs. 3%, respectively; P = 0.039). Patients with tonsil cancer showed more frequent HPV positivity than tongue cancer (42% vs. 2%, respectively; P < 0.001). There was no association between HPV positivity and EGFR mutation. In terms of disease-free survival, patients with EGFR mutation had better survival independently of stage and primary site (hazard ratio, 0.246; 95% CI, 0.075 to 0.802). Conclusions: We did not find association between EGFR mutation and HPV positivity. Patients with EGFR mutation showed better outcome in tongue and tonsil cancer. These results suggest that EGFR mutation may be a prognostic factor in patients with head and neck cancer. No significant financial relationships to disclose.

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