Isolation and Characterization of Avian Influenza Virus Based on Macroscopic Examination of Native Chicken Embryo

The aim of this research was to isolate and characterize the virus based on macroscopic examination of native chickens embryo infected with the AI virus. This research used 10 infected native chickens from Ateuk Pahlawan and Doi villages. All chickens were examined to identify the present of AI using rapid test and in ovo virus propagation. Rapid test showed 7 chickens from Ateuk Pahlawan villageand 5 chickens from Doi village were AI positive. Propagation of the viruses in 11 days old chicken embryo in ovo showed that embryo died 5 days post inoculated whereas macroscopic examination showed dwarfed embryo, hemorrhagic, and loss of feather.

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Isolation and Characterization of a Novel H9N2 Influenza Virus in Korean Native Chicken Farm

Avian Diseases Digest ◽

10.1637/9925-977411-digest.1 ◽

2011 ◽

Vol 6 (4) ◽

pp. e68-e69

Author(s):

Yu-Na Lee ◽

Dong-Hun Lee ◽

Jae-Keun Park ◽

Tea-Hyun Lim ◽

Ha-Na Youn ◽

...

Keyword(s):

Influenza Virus ◽

H9n2 Influenza Virus ◽

Isolation And Characterization ◽

Native Chicken ◽

Chicken Farm ◽

Korean Native Chicken

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Isolation and Characterization of a Novel H9N2 Influenza Virus in Korean Native Chicken Farm

Avian Diseases ◽

10.1637/9774-050911-case.1 ◽

2011 ◽

Vol 55 (4) ◽

pp. 724-727 ◽

Cited By ~ 17

Author(s):

Yu-Na Lee ◽

Dong-Hun Lee ◽

Jae-Keun Park ◽

Tae-Hyun Lim ◽

Ha-Na Youn ◽

...

Keyword(s):

Influenza Virus ◽

H9n2 Influenza Virus ◽

Isolation And Characterization ◽

Native Chicken ◽

Chicken Farm ◽

Korean Native Chicken

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Avian influenza and Newcastle disease virusindead chickens in markets in Dhaka, Bangladesh in 2011-2012

Bangladesh Veterinarian ◽

10.3329/bvet.v33i1.33308 ◽

2017 ◽

Vol 33 (1) ◽

pp. 8-15

Author(s):

LR Barman ◽

RD Sarker ◽

BC Das ◽

EH Chowdhury ◽

PM Das ◽

...

Keyword(s):

Influenza Virus ◽

Avian Influenza ◽

Newcastle Disease ◽

Rapid Test ◽

Type A ◽

Antigen Test ◽

Rt Pcr ◽

Test Kit ◽

Live Bird

A virological survey for avian influenza (AI) and Newcastle disease (ND) was conducted in two selected live bird markets (LBMs), namely Kaptan Bazar and Karwan Bazar in Dhaka city, Bangladesh from August 2011 to July 2012. A total of 513 dead chickens were collected. An immune-chromatographic rapid antigen test for Type A influenza virus and both conventional and real time RT-PCR were used for the detection and characterization of AI and ND viruses. All carcasses were first screened by the rapid antigen test kit and 93 were positive for Type A influenza virus. RT-PCR on a representative number of rapid antigen test positive samples (n = 24) confirmed the presence of Type A influenza virus and mostly H5 influenza virus (22 out of 24 tested samples). Influenza rapid test negative samples (n = 420) were subjected to routine necropsy. Heat stress, suffocation and physical injury were the most common cause of mortality (163 cases), followed by ND, suspected to be the cause of 85 deaths. On molecular investigation of these 85 samples, the presence of ND virus was confirmed in 59 and AI virus in 6; 15 were negative for both ND and AI viruses and 5 were unsuitable for investigation. Among the 59 ND confirmed cases 18 also contained AI virus. In summary, out of 513 carcasses 117 (22.81%) contained AI virus and 59 (11.50%) contained ND virus. Eighteen (3.51%) carcasses contained both AI and ND viruses. The findings suggest that both AI and ND should be considered as major threats to the poultry industry.Bangl. vet. 2016. Vol. 33, No. 1, 8-15

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