scholarly journals Flow Injection Determination of Xanthine Oxidase Inhibitory Activity and Its Application to Food Samples

2006 ◽  
Vol 22 (1) ◽  
pp. 105-109 ◽  
Author(s):  
Le Hoang LAM ◽  
Ken SAKAGUCHI ◽  
Hiroyuki UKEDA ◽  
Masayoshi SAWAMURA
2021 ◽  
Author(s):  
Syarifah Ramadhani Lubis ◽  
Subandi Subandi ◽  
Muntholib Muntholib ◽  
Jamilah Abbas ◽  
Tjandrawati Mozef

1993 ◽  
Vol 76 (6) ◽  
pp. 1255-1261 ◽  
Author(s):  
A Espinosa-Mansilla ◽  
A Muñoz Dela Peña ◽  
F Salinas

Abstract A kinetic study of the reactions of 5-hydroxymethyl-2-furfuraldehyde and furfural with 2-thiobarbituric acid (TBA) by a stopped-flow flow injection analysis technique has been undertaken. A semiautomatic method for the analytical determination of these furanic aldehydes is proposed on the basis of reaction with TBA. The proposed stopped-flow method was successfully applied to several commercial pharmaceutical preparations and food samples. The procedure is faster than the earlier procedure for determination of these compounds in foods and pharmaceuticals.


2003 ◽  
Vol 24 (1213) ◽  
pp. 2092-2098 ◽  
Author(s):  
Bartolomé M. Simonet ◽  
Angel Ríos ◽  
Félix Grases ◽  
Miguel Valcárcel

2003 ◽  
Vol 15 (7) ◽  
pp. 601-607 ◽  
Author(s):  
L. Agüí ◽  
A. Guzman ◽  
M. Pedrero ◽  
P. Yáñez-Sedeño ◽  
J. M. Pingarrón

2001 ◽  
Vol 79 (3) ◽  
pp. 337-341
Author(s):  
Hong Yeob Choi ◽  
Jin Hyang Song ◽  
Yong Sung Park ◽  
Gabriel Lord ◽  
Dong Ki Park

Superoxide dismutase (SOD) activity was measured using a flow injection-chemiluminescent assay (FI-CLA) based on xanthine–xanthine oxidase dependent superoxide (O2.–) formation. The mobile phase consists of 50 mM potassium phosphate buffer (pH 7.4) containing lucigenin (5 µM) and xanthine (0.3 mM). Under optimum conditions, bovine albumin did not affect chemiluminescence at concentrations of 1–1000 µg mL–1 and KCN inhibited 100% of the Cu, Zn-SOD activity using 5 µL of a 0.23 mM concentration. The analysis of one sample was done in less than 30 s with a relative standard deviation of ±3.1%. SOD activity in the biological samples was correlated to the amount of exogenously applied SOD. The FI-CLA method reported here appears to be one of the faster and more useful tools used to assay SOD activity.Key words: superoxide dismutase, flow injection-chemiluminescent assay, superoxide, xanthine oxidase, lucigenin.


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