Antioxidant activity, xanthine oxidase inhibitory activity, and compounds determination of Cipadessa baccifera leaf extract

Objective: The purpose of this research was to determine antioxidant activity of green tea leaf extract, the value of sun protection factor (SPF),physical properties, and stability of green tea cream.Methods: Green tea leaf extract was obtained by maceration using 96% ethanol. Cream was prepared in three formulas with various concentrationof the green tea leaves extract. The physical evaluations included organoleptic, pH, viscosity, adhesion, spreadability, and stability tests weredone. The determination of the SPF value is calculated using the Mansur equation. Extract of green tea leaf has strong antioxidant activity(IC50 2.19 μg/ml).Results: The green tea leaf extract showed high antioxidant activity (2.19 μg/m). All formulas are organoleptically creamy brownish-green to brown,with a distinctive green tea odor and homogeneous. All formulas met the requirements of physical properties of cream. The creams showed significantchange while they were stored at 4°C and at 40±2°C, but showed no difference when they were stored ad 26°C. SPF values of cream are 0.54; 2.03,and 2.41, respectively.Conclusions: It is clearly indicated that the sunscreen cream of green tea leaf extract is potential to be further developed as cosmetic preparations.

Download Full-text

Antioxidant Activity Test of Effervescent Granules Morinda citrifolia L Leaf Extract with DPPH Free Radical Absorption Method

Journal Of Tropical Pharmacy And Chemistry ◽

10.25026/jtpc.v5i4.285 ◽

2021 ◽

Vol 5 (4) ◽

pp. 365-368

Author(s):

Muthmainah Tuldjanah ◽

Niluh Puspita Dewi ◽

Dian Rahmawati

Keyword(s):

Antioxidant Activity ◽

Free Radical ◽

Leaf Extract ◽

Natural Antioxidants ◽

Morinda Citrifolia ◽

Absorption Method ◽

Immersion Method ◽

Activity Test ◽

Ic50 Values

Morinda citrifolia. L leaf is a plant that has the potential as an antioxidant due to the presence of compounds in the form of flavonoids and other phenolic compounds that can function as natural antioxidants. In connection with this, research was carried out to Morinda citrifolia. L formulate leaf extract in the form of effervescent granules. Morinda citrifolia leaf extract was macerated with ethanol 96% as solvent. Phytochemical screening showsthat the extract contains flavonoids, tannins, saponins and alkaloids. The extract was formulated with various concentrations of F1 (20%), F2 (25%), F3 (4%). Determination of antioxidant activity using the DPPH free radical immersion method. The results showed that the concentration in the granules influenced antioxidant activity with IC50 values of 109.05 ppm, 101.33 ppm and 73.28 ppm, respectively

Download Full-text

Potential of Miana Leaf Extract as Expectorant (Profile Place of Growing, Antioxidant, Sputum Contaminants, Antibacterial, MIC, MKC Expectorant)

Majalah Obat Tradisional ◽

10.22146/mot.52500 ◽

2020 ◽

Vol 25 (2) ◽

pp. 94

Author(s):

Sesilia Rante Pakadang ◽

Santi Sinala ◽

Alfrida Monica Salasa ◽

St Ratnah ◽

Sisilia Teresia Rosmala Dewi ◽

...

Keyword(s):

Antioxidant Activity ◽

Effective Dose ◽

Leaf Extract ◽

Sputum Sample ◽

Dilution Method ◽

Klebsiella Pneumonia ◽

Enterobacter Agglomerans ◽

Dpph Method

Research has been conducted on the treatment of phlegm cough with miana leaf extract in vitro (effective dose of miana leaf extract as an expectorant and antibacterial agent causing cough with phlegm). The study aims to compare the antioxidant activity of miana leaves from 3 locations where it grows, determine the types of contaminant bacteria in the sputum of cough sufferers, determine the minimum value of inhibitor concentration (MIC) and MKC of miana leaves against the test bacteria causing cough with phlegm, determine the effective dose of miana leaves that can used as a reference for coughing up phlegm and proving the potential of miana leaves as a sputum thinner. Miana leaf extraction is done by the juicer method. Antioxidant activity testing uses the DPPH method. Determination of test bacteria is done by isolating and identifying contaminant bacteria in the sputum sample of cough with phlegm. Testing the effectiveness of miana leaves against test bacteria is determined by the liquid dilution method. Expectorant activity testing was determined by measuring the viscosity of mucus viscosity of cow intestine treated with miana leaf extract. The results found that antioxidant activity was influenced by the location where miana leaves grew with an antioxidant potential of IC72 0.072 mg/ml - 0.76 mg/ml. Contaminant bacteria from sputum samples of cough patients are Streptococcus pneumonia, Klebsiella pneumonia, Staphylococcus aureus, Staphylococcus epidermidis and Enterobacter agglomerans. MIC values for contaminant bacteria are 0.1% - 0.75% and MKC values are found between 0.25% - 1.75%. miana leaf extract has the potential as a sputum thinner at a concentration of 0.01% - 0.1%. The recommended dose of miana leaf extract as a cough with phlegm is 1.75% w/v.

Download Full-text

IN VITRO AND IN SILICO EVALUATION OF XANTHINE OXIDASE INHIBITORY ACTIVITY OF QUERCETIN CONTAINED IN SONCHUS ARVENSIS LEAF EXTRACT

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10s2.19486 ◽

2017 ◽

Vol 10 (14) ◽

pp. 50

Author(s):

Rini Hendriani ◽

Nursamsiar Nursamsiar ◽

Ami Tjitraresmi

Keyword(s):

Free Energy ◽

Xanthine Oxidase ◽

Inhibitory Activity ◽

In Silico ◽

Leaf Extract ◽

Binding Free Energy ◽

Uv Spectrophotometry ◽

Docking Simulations ◽

Oxidase Enzyme

Objective: The aim of the present study was to examine the inhibiting effects of quercetin contained in Sonchusarvensis leaf extract on the activity of xanthine oxidase, an essential enzyme for uric acid synthesis.Methods: Activity test was conducted in vitro by measuring the activity of xanthine oxidase using UV spectrophotometry and in silico by determining the interaction of quercetin and allopurinol (as comparation drug) with xanthine oxidase enzyme in terms of hydrogen bonds and binding free energy. Docking simulations were performed by Autodock4.2 package.Results: The active fraction, using the solvent n-hexane, ethyl acetate and water, tested the inhibitory activity of the xanthine oxidase enzyme in vitro obtained respectively IC50 of 263.19, 16.20 and 141.80 μg/ml. Isolates with highest activity identified as quercetin. The xanthine oxidase enzyme inhibitory activity insilico by molecular docking showed quercetin has free energy binding ˗7.71 kcal/mol, more negative than that of allopurinol ˗5.63 kcal/mol.Conclusion: This shows the affinity of quercetin stronger than that of allopurinol; so that it can be predicted that quercetin was more potential to inhibit xanthine oxidase enzyme activity. Thus the extract of the S. arvensis leaves containing the active compound quercetin was a potential use as antihyperuricemia.

Download Full-text

DETERMINATION OF PHYTOCHEMICALS, ANTIOXIDANT ACTIVITY AND BIOCHEMICAL COMPOSITION OF CHINESE MUGWORT (ARTEMISIA ARGYI L.) LEAF EXTRACT FROM NORTHEAST CHINA

Applied Ecology and Environmental Research ◽

10.15666/aeer/1706_1534915362 ◽

2019 ◽

Vol 17 (6) ◽

Author(s):

M AHMED

Keyword(s):

Antioxidant Activity ◽

Biochemical Composition ◽

Northeast China ◽

Leaf Extract ◽

Artemisia Argyi

Download Full-text

Spectrophotometric Determination of Total Flavonoid Content in Biancaea Sappan (Caesalpinia sappan L.) Leaves

Jurnal Fitofarmaka Indonesia ◽

10.33096/jffi.v8i3.712 ◽

2021 ◽

Vol 8 (3) ◽

pp. 1-4

Author(s):

Nurlinda Nurlinda ◽

Virsa Handayani ◽

Faradiba Abdul Rasyid

Keyword(s):

Antioxidant Activity ◽

Leaf Extract ◽

Methanol Extract ◽

Total Flavonoid ◽

Total Flavonoids ◽

Total Flavonoid Content ◽

Flavonoid Content ◽

Caesalpinia Sappan ◽

Antimicrobial Treatments

AbstractBiancaea sappan (BS) is traditionally used to treat anticonvulsants, anti-inflammatory, antiproliferative, anticoagulant, antiviral, immunostimulant, antioxidant, and antimicrobial treatments. Flavonoids are found in Secang; flavonoids are secondary metabolites that have antioxidant activity. This study aims to identify the flavonoids using TLC and determination of flavonoids content in BS leaves. Initially, The methanol extract of BS was obtained by maceration with ethanol. The qualitative analysis of flavonoid was using TLC and visualization by sprayed with AlCl3. The determination of total flavonoid content is conducted based on the AlCl3 method with total flavonoids expressed in QE (Quercetin equivalent) at the maximum wavelength of 431 nm. The research results showed that BS leaves contain flavonoids and the total flavonoid content of BS leaf extract is 1.0318 mg QE / g extract.Keywords: Caesalpinia sappan; Flavonoid content, Spectrophotometric

Download Full-text

Comparative Study of Xanthine Oxidase Inhibitory Activity Of Methanolic Leaf Extracts Of CalophyllumBlancoi (Bitaog), Diospyrospilosanthera (Bolongeta) and SyzygiumCumini (Duhat)

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11ispl4.3894 ◽

2020 ◽

Vol 11 (SPL4) ◽

pp. 531-537

Author(s):

Jan Asuncion ◽

Mariane May Domingo ◽

Rave Harvey Sienna ◽

ZhaineMarille Villa ◽

Jennifer Anne Loyola

Keyword(s):

Xanthine Oxidase ◽

Inhibitory Activity ◽

Leaf Extract ◽

Leaf Extracts ◽

Standard Drug ◽

Ic50 Value ◽

Vitro Analysis ◽

In Vitro Analysis

Gout is characterized as an inflammation and warmth in the joints. It is associated with hyperuricemia wherein an upregulation of xanthine oxidase in purine degradation leads to increased levels of uric acid in the blood. Gout is not fatal. However, it affects one’s quality of life. Thus, this research primarily focuses in determining the inhibitory activity of xanthine oxidase in the methanolic leaf extract of bitaog (Calophyllumblancoi), bolongeta (Diospyrospilosanthera), and duhat (Syzygiumcumini) in gout. A quantitative-experimental research method was used in the study and the data were obtained by measuring the percent inhibition of the samples using UV-Vis spectrophotometer at 290 nm. The results showed that the methanolic leaf extract of above stated plants exhibited exemplary inhibition in comparison with the standard drug, allopurinol. The IC50 value determines the ability of the inhibitor to decrease the biotransformation of a substrate. The principle behind IC50 is, the lower the value the higher the inhibition. The bitaog (Calophyllumblancoi) trials have the lowest IC50 value with an average of 124.3 after the standard drug, followed by bolongeta (Diospyrospilosanthera) have an average of 155.3 IC50 value. Then duhat (Syzygiumcumini) showed the highest IC50 an average of 208.8. The bitaog (Calophyllumblancoi), next to allopurinol, showed the highest inhibition among all the extracts followed by the bolongeta (Diospyrospilosanthera). The least inhibitory activity was observed in duhat (Syzygiumcumini). Hence, it can be concluded that bitaog (Calophyllumblancoi), bolongeta (Diospyrospilosanthera), and duhat (Syzygiumcumini) can inhibit xanthine oxidase using in vitro analysis.

Download Full-text