scholarly journals Analysis of poly-functional acrylates and methacrylates by reversed-phase liquid chromatography with photodiode-array detector.

1992 ◽  
Vol 41 (1) ◽  
pp. 29-35
Author(s):  
Hatsuo SUGITANI ◽  
Masato FUKASAWA ◽  
Kouichi KATOU
Keyword(s):  
Liquid Chromatography ◽  
Reversed Phase ◽  
Array Detector ◽  
Reversed Phase Liquid Chromatography ◽  
Photodiode Array Detector ◽  
Photodiode Array ◽  
Phase Liquid

scholarly journals Quantitative Analysis of Forskolin in Coleus forskohlii (Lamiaceae) by Reversed-Phase Liquid Chromatography

2003 ◽  
Vol 86 (3) ◽  
pp. 467-470 ◽  
Author(s):  
Brian T Schaneberg ◽  
Ikhlas A Khan
Keyword(s):  
Liquid Chromatography ◽  
Limit Of Detection ◽  
Reversed Phase ◽  
Array Detector ◽  
Coleus Forskohlii ◽  
Reversed Phase Liquid Chromatography ◽  
Mass Spectrometry Experiment ◽  
Marker Compound ◽  
Photodiode Array Detector ◽  
Phase Liquid

Abstract A rapid method was developed for the evaluation of forskolin in Coleus forskohlii Briq. (Lamiaceae). Forskolin was quantitated in the root and stem of dried C. forskohlii and in 17 market products by reversed-phase liquid chromatography (LC) with a photodiode array detector at 210 nm. The temperature was held constant at 30°C, and the retention time of forskolin was approximately 6.8 min. The samples were extracted with acetonitrile by sonication. The precision of the method was confirmed by a standard deviation <5.0% (n = 3), and forskolin recovery was 99.1%. Limit of detection was 1.5 μg/mL, and the response was linear through zero from 6.3 to 630 μg/mL with a correlation coefficient (R2) of 0.9998. Identity of the marker compound was confirmed by an LC/mass spectrometry experiment. The method was successful in the qualitative and quantitative evaluation of the marker compound in C. forskohlii plant material and in market products claiming to contain C. forskohlii.

scholarly journals ESTIMATION OF GUGGULSTERONE-Z IN GOKSHURADI GUGGULU USING REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

2018 ◽  
Vol 11 (11) ◽  
pp. 204
Author(s):  
Kanan G Gamit ◽  
Niraj Y Vyas ◽  
Nishit D Patel ◽  
Manan A Raval
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Reversed Phase ◽  
Array Detector ◽  
Limit Of Quantification ◽  
Photodiode Array Detector ◽  
Validation Parameters ◽  
Photodiode Array

Objective: A study was aimed to estimate guggulsterone-Z (GZ) in Gokshuradi Guggulu (GG).Methods: An analytical method was developed and validated using Waters Alliance high-performance liquid chromatography system (Empower software), equipped with photodiode array detector. Separation was achieved using Phenomenex, C-18 (250 mm×4.6 mm, 5 μ) column. Mobile phase consisted of acetonitrile:water (70:30,v/v). Flow rate was set to 1 ml/min and detection was performed at 251 nm.Results and Discussion: Validation parameters such as linearity, precision, accuracy, limit of detection, limit of quantification, and robustness were performed. Amount of GZ was estimated using linearity equation.Conclusion: GG was found to contain 0.815±0.03 g% w/w GZ. Validated method may be used as one of the parameters to standardize the formulation.

scholarly journals Quantitative Determination of Usnic Acid in Usnea lichen and Its Products by Reversed-Phase Liquid Chromatography with Photodiode Array Detector

2005 ◽  
Vol 88 (5) ◽  
pp. 1265-1268 ◽  
Author(s):  
Xiuhong Ji ◽  
Ikhlas A Khan
Keyword(s):  
Liquid Chromatography ◽  
Weight Control ◽  
Limit Of Detection ◽  
Usnic Acid ◽  
Reversed Phase ◽  
Array Detector ◽  
Plant Materials ◽  
Photodiode Array Detector ◽  
Wide Range ◽  
Photodiode Array

Abstract Usnic acid, a lichen substance, has a wide range of pharmaceutical applications, including antibiotic, antimycotic, antifeedant, antitubercular, antitumor, and analgesic activities. Some products containing usnic acid are marketed as weight control supplements; however, hepatotoxicity and acute liver failures were reported as severe side effects. The usnic acid content present in the plant materials and market products was analyzed by reversed-phase high-pressure liquid chromatography with a photodiode array detector at 233 nm. A Waters XTerra RP18 (150 × 4.6 mm; 5 μm particle size) column was the stationary phase; mobile phase was aqueous 0.1% acetic acid and acetonitrile gradient at flow rate of 1.0 mL/min. The temperature was held constant at 30°C. The retention time of usnic acid was approximately 13.3 min. Acetone extraction of the samples took place with sonication. The precision of the method was confirmed by a standard deviation below 3.0% (n = 3) and usnic acid recovery was 99.0%. Limit of detection was 0.4 μg/mL and the response was linear from 1.4 to 570.0 μg/mL with a correlation coefficient (R2) of 0.9991. The content of usnic acid in 4 raw materials and 22 finished products was analyzed.

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