Gene EBNA3C: Type of Infection by EBV (EBV1 and EBV2) Correlation With Clinical and Biochemical Parameters (AST, ALT and GGT) in Individuals With Infectious Mononucleosis in the Metropolitan Area of Belém, Pará, 2005-2016

Abstract Background: Two types of Epstein Barr virus (EBV1 and 2) have been shown to infect humans. This study aimed to detect the types of EBV that cause infectious mononucleosis and correlate these viral types with clinical parameters in the metropolitan region of Belém from 2005 to 2016.Methods: A total of 76 cases of infectious mononucleosis (IM) were processed at the Instituto Evandro Chagas, Ananindeua, Brazil. PCR was used to analyze the EBNA 3C region for the recognition of EBV types. Biochemical testing (aspartate aminotransferase (AST), alanine aminotransferase (ALT) and gamma-glutamyl transferase (GGT) was performed by the COBAS INTEGRA clinical biochemistry PLUS 400 / ROCHE automatic analyzer. The data were evaluated using the Statistical Package for Social Science - SPSS 17.0 and GraphPadPrism 7.0 for Windows.Results: EBV1 infection was observed in 71.1% (54/76) of individuals, among whom those > 14 years constituted 66.7% (36/54); the average age was 23 years, and the number of women infected was higher (61.1% [33/54]) than that of men 38.9%[21/54]). The symptoms/clinical signs observed in infection by EBV1 were cervical lymphadenopathy in 64.8% (35/54), fever in 63% (34/54), headache and arthralgia in 20.3% (11/54), and exanthema in 18.5% (10/54). Infection by EBV2 was observed in only 17.1% (13/76) of cases. Coinfections by EBV1 and EBV2, most frequently showing symptoms of fever and cervical lymphadenopathy, occurred in 66.7% (6/9) and 55.6% (5/9) of individuals. Alterations to AST were confirmed in 14.8% (8/54), EBV2 of cases in 7.7% (1/13) in EBV1 infection. Conclusions: EBV1 was predominant in 71% of clinical cases of infectious mononucleosis. The correlation of biochemical parameters in infection by EBV1, EBV2, and coinfections by EBV1/2 revealed a statistically significant difference in mean changes of EBV1 in individuals older than 14 years.

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Epstein Barr virus genotypes (EBV1/EBV2) in individuals with infectious mononucleosis in the metropolitan area of Belém, Brazil, between 2005 and 2016

10.21203/rs.3.rs-16300/v1 ◽

2020 ◽

Author(s):

Talita Antonia Furtado Monteiro ◽

Iran Barros Costa ◽

Igor Brasil Costa ◽

Thais Letícia dos Santos Corrêa ◽

...

Keyword(s):

Infectious Mononucleosis ◽

Epstein Barr Virus ◽

Health Care Service ◽

Clinical Signs ◽

Cervical Lymphadenopathy ◽

Metropolitan Region ◽

Gamma Glutamyl Transferase ◽

Barr Virus ◽

Epstein Barr ◽

Glutamyl Transferase

Abstract Background: Two types of Epstein Barr virus (EBV1/EBV2) have been shown to infect humans by causing infectious mononucleosis, their genome being very similar, except for regions of the EBNA genes. This study aimed to describe the EBV genotypes in cases of infectious mononucleosis in the metropolitan region of Belém, Brazil, from 2005 to 2016. Methods: A total of 8.295 suspected cases with symptoms/signs of infectious mononucleosis (MI) were investigated by doctors of infectious diseases at the Evandro Chagas Institute Health Care Service from January 2005 to December 2016. In 3.0% (251/8.295) of the cases were positive by enzymatic immunoassay were submitted to PCR for EBNA3C region to detect the types of EBV. Biochemical testing involving aspartate aminotransferase, alanine aminotransferase and gamma-glutamyl transferase were realized. Results: The identification of EBV types by PCR was verified in 30.3% (76/251) of individuals, being 71.1% (54/76) classified as EBV1, 17.1% (13/76) as EBV2 and 11.8% (9/76) as EBV1+EBV2. The number of women infected with EBV1 was higher (61.1% - 33/54) than for men (38.9% - 21/54), most were over 14 years old (66.7%-36/54). The main symptoms/clinical signs observed in EBV1 infection were: cervical lymphadenopathy (64.8%-35/54), fever (63%-34/54), headache (20.3%-11/54), arthralgia (20.3%-11/54) and exanthema (18.5%-10/54). In EBV2 infection, it was also detected in all age groups, with the exception of two groups, with an average age of 24 years. The presence of fever in 76.9% (10/13) with an average duration of 18 days and lymphadenopathy in 53.8 (7/13) were the most relevant signs/symptoms in EBV2. In contrast, EBV1+EBV2 co-infection was more frequent in the £5 year age group, affecting 20.0% (2/10). Women presented 66.7% (6/9) more positive cases. The symptoms involving EBV1+EBV2 co-infection were more related to fever (66.7%-6/9) and cervical lymphadenopathy (55.6% -5/9). The average of enzymatic values according to type of EBV was statistically significant (p <0.05) in individuals with EBV1 infection in those over 14 years of age. Conclusions: A pioneering study that molecularly identified the genotypes of EBV in 30.3% of cases, with circulation of EBV1, EBV2 and co-infection EBV1+EBV2 in cases of infectious mononucleosis in the northern region of Brazil.

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Gene EBNA3C: Type of Infection by EBV (EBV1 and EBV2) Correlation With Clinical and Biochemical Parameters (AST, ALT and GGT) in Individuals With Infectious Mononucleosis in the Metropolitan Area of Belém, Pará, 2005-2016

10.21203/rs.2.10515/v3 ◽

2019 ◽

Author(s):

Talita Antonia Furtado Monteiro ◽

Iran Barros Costa ◽

Igor Brasil Costa ◽

Thais Letícia dos Santos Corrêa ◽

Beatriz Monteiro Rodrigues Coelho ◽

...

Keyword(s):

Social Science ◽

Infectious Mononucleosis ◽

Metropolitan Area ◽

Biochemical Parameters ◽

Clinical Biochemistry ◽

Clinical Signs ◽

Cervical Lymphadenopathy ◽

Metropolitan Region ◽

Significant Difference ◽

Biochemical Testing

Abstract Background: Two types of EBV (EBV1 and 2) have been shown to infect humans. This study aimed to detect the types of EBV that cause infectious mononucleosis and correlate these viral types with biochemical parameters (AST, ALT and GGT) in the metropolitan region of Belém from 2005 to 2016. Methods: A total of 76 cases of infectious mononucleosis were processed at the Instituto Evandro Chagas, Ananindeua, Brazil. PCR was used to analyze the EBNA3C region for the recognition of EBV types. Biochemical testing (AST, ALT and GGT) was performed by the COBAS INTEGRA clinical biochemistry PLUS 400 / ROCHE automatic analyzer. The data were evaluated using the Statistical Package for Social Science - SPSS 17.0 and GraphPadPrism 7.0 for Windows (GraphPad software, San Diego, CA, USA). Results: EBV1 infection was observed in 71.1% (54/76) of individuals, among whom those > 14 years constituted 66.7% (36/54); the average age was 23 years, and the number of women infected was higher (61.1% [33/54]) than that of men 38.9%[21/54]). The symptoms/clinical signs observed in infection by EBV1 were cervical lymphadenopathy in 64.8% (35/54), fever in 63% (34/54), headache and arthralgia in 20.3% (11/54), and exanthema in 18.5% (10/54). Infection by EBV2 was observed in only 17.1% (13/76) of cases. Coinfection by EBV1 and EBV2, most frequently showing symptoms of fever and cervical lymphadenopathy, occurred in 66.7% (6/9) and 55.6% (5/9) of individuals. Conclusion: EBV1 was predominant in 71% of clinical cases of infectious mononucleosis. The correlation of biochemical parameters in infection by EBV1, EBV2, and coinfection by EBV1/2 revealed a statistically significant difference in mean changes of EBV1 in individuals older than 14 years.

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GENE EBNA3C: TYPES OF INFECTION BY EBV (EBV1 and EBV2), CORRELATION WITH CLINICAL AND BIOCHEMICAL PARAMETERS (AST, ALT and GGT) IN INDIVIDUALS WITH INFECTIOUS MONONUCLEOSIS OF THE METROPOLITAN AREA OF BELÉM, PARÁ, IN THE PERIOD OF 2005-2016

10.21203/rs.2.10515/v2 ◽

2019 ◽

Author(s):

Talita Antonia Furtado Monteiro ◽

Iran Barros Costa ◽

Igor Brasil Costa ◽

Thais Letícia dos Santos Corrêa ◽

Beatriz Monteiro Rodrigues Coelho ◽

...

Keyword(s):

Infectious Mononucleosis ◽

Metropolitan Area ◽

Biochemical Parameters ◽

Clinical Signs ◽

Cervical Lymphadenopathy ◽

Metropolitan Region ◽

Significant Difference ◽

Clinical Cases

Abstract Two types of EBV (EBV1 and 2) have been shown to infect humans. Detect the types of EBV that cause infectious mononucleosis and correlate these viral types with biochemical parameters (AST, ALT and GGT) on in the metropolitan region of Belém, 2005 to 2016. A total 76 cases of infectious mononucleosis, processed the Instituto Evandro Chagas, Ananindeua, Brazil. Evaluated by PCR used primers the region EBNA 3C. EBV1 infection was observed in 71.1% (54/76) of individuals, among whom those > 14 years constituted 66.7% (36/54), the average age was 23 years, and the number of women infected was higher (61.1% [33/54]) than that of men (38.9%[21/54]). The symptoms/clinical signs observed in infection by EBV1 were cervical lymphadenopathy in 64.8% (35/54), fever in 63% (34/54), headache and arthralgia in 20.3% (11/54), and exanthema in 18.5% (10/54). Infection by EBV2 was observed in only 17.1% (13/76) of cases. Co-infection by EBV1 and EBV2, most frequently showing symptoms of fever and cervical lymphadenopathy, occurred in 66.7 (6/9) and 55.6% (5/9) of individuals. EBV1 was predominant in 71% of clinical cases of infectious mononucleosis. Correlation of biochemical parameters in infection by EBV1, EBV2, and co-infection by EBV1/2 revealed a statistically significant difference in mean changes of EBV1 in individuals older than 14 years.

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GENE EBNA3C: TYPES OF INFECTION BY EBV (EBV1 and EBV2), CORRELATION WITH CLINICAL AND BIOCHEMICAL PARAMETERS (AST, ALT and GGT) IN INDIVIDUALS WITH INFECTIOUS MONONUCLEOSIS OF THE METROPOLITAN AREA OF BELÉM, PARÁ, IN THE PERIOD OF 2005-2016

10.21203/rs.2.10515/v1 ◽

2019 ◽

Author(s):

Talita Antonia Furtado Monteiro ◽

Iran Barros Costa ◽

Igor Brasil Costa ◽

Thais Letícia dos Santos Corrêa ◽

Beatriz Monteiro Rodrigues Coelho ◽

...

Keyword(s):

Infectious Mononucleosis ◽

Metropolitan Area ◽

Biochemical Parameters ◽

Clinical Signs ◽

Cervical Lymphadenopathy ◽

Metropolitan Region ◽

Significant Difference ◽

Clinical Cases

Abstract Two types of EBV (EBV1 and 2) have been shown to infect humans. Detect the types of EBV that cause infectious mononucleosis and correlate these viral types with biochemical parameters (AST, ALT and GGT) on in the metropolitan region of Belém, 2005 to 2016. A total 76 cases of infectious mononucleosis, processed the Instituto Evandro Chagas, Ananindeua, Brazil. Evaluated by PCR used primers the region EBNA 3C. EBV1 infection was observed in 71.1% (54/76) of individuals, among whom those > 14 years constituted 66.7% (36/54), the average age was 23 years, and the number of women infected was higher (61.1% [33/54]) than that of men (38.9%[21/54]). The symptoms/clinical signs observed in infection by EBV1 were cervical lymphadenopathy in 64.8% (35/54), fever in 63% (34/54), headache and arthralgia in 20.3% (11/54), and exanthema in 18.5% (10/54). Infection by EBV2 was observed in only 17.1% (13/76) of cases. Co-infection by EBV1 and EBV2, most frequently showing symptoms of fever and cervical lymphadenopathy, occurred in 66.7 (6/9) and 55.6% (5/9) of individuals. EBV1 was predominant in 71% of clinical cases of infectious mononucleosis. Correlation of biochemical parameters in infection by EBV1, EBV2, and co-infection by EBV1/2 revealed a statistically significant difference in mean changes of EBV1 in individuals older than 14 years.

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Biochemical effects induced by deoxynivalenol intoxication in piglets

Biotechnology in Animal Husbandry ◽

10.2298/bah0702245d ◽

2007 ◽

Vol 23 (5-6-2) ◽

pp. 245-250 ◽

Cited By ~ 1

Author(s):

A. Dinischiotu ◽

D. Dinu ◽

M. Rebedea ◽

G. Stoian ◽

I. Taranu ◽

...

Keyword(s):

Biochemical Parameters ◽

Gamma Glutamyl Transferase ◽

Serum Urea ◽

Serum Total Protein ◽

Biochemical Effects ◽

Significant Difference ◽

Serum Biochemical ◽

Glutamyl Transferase ◽

Aspartate Transferase ◽

Sera Samples

Thirty-5 weeks-old pigs were fed corn-soybean diets containing 0.5 ppm and 1.5 ppm. deoxynivalenol. Sera samples were collected from ten piglets in each group at the end of 35 days of the trial to study the effect of certain serum biochemical parameters. Highly significant (P < 0.05) differences were observed for serum urea and gamma glutamyl transferase between control and mycotoxin treated groups. Mycotoxin treated groups did not reveal any significant difference for serum total protein, albumin, globulin, aspartate transferase and alanine transferase.

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EBV Infection Unmasking a Choledochal Cyst in an Infant

Case Reports in Pediatrics ◽

10.1155/2019/7320109 ◽

2019 ◽

Vol 2019 ◽

pp. 1-3

Author(s):

Hassan El Khatib ◽

Batoul Kawtharany ◽

Diyaa Mohammad ◽

Mohammad Siblini ◽

Nahida El-Rifai

Keyword(s):

Choledochal Cyst ◽

Stone Formation ◽

Liver Function Tests ◽

Gamma Glutamyl Transferase ◽

Barr Virus ◽

Ebv Infection ◽

Hepatic Involvement ◽

Epstein Barr ◽

Glutamyl Transferase

Hepatic involvement is common in acute Epstein–Barr virus (EBV) infection in children. It usually manifests as a transitory elevation of transaminases in up to 80% to 90% of patients, and they normalize by 2 to 6 weeks. A cholestatic pattern with elevated gamma-glutamyl transferase (γGT) and alkaline phosphatase (ALP) is common, in up to 60% in young adults. However, jaundice is very rare occurring in only 5% of pediatric patients. We report here an 8-month-old female with EBV infection who developed obstructive jaundice 2 weeks after the initial infection. Radiologic investigations were compatible with choledochal cyst type IVa complicated by stone formation in the common bile duct. In case of clinical exacerbation or nonamelioration of liver function tests in EVB infection, another diagnosis should be addressed. This highlights the importance of close follow-up in these patients in order not to miss a serious underlying condition such as choledochal malformation.

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Analysis of the Variability of Epstein-Barr Virus Genes in Infectious Mononucleosis: Investigation of the Potential Correlation with Biochemical Parameters of Hepatic Involvement

Journal of Medical Biochemistry ◽

10.1515/jomb-2015-0021 ◽

2016 ◽

Vol 35 (3) ◽

pp. 337-346

Author(s):

Ana Banko ◽

Ivana Lazarevic ◽

Goran Stevanovic ◽

Andja Cirkovic ◽

Danijela Karalic ◽

...

Keyword(s):

Infectious Mononucleosis ◽

Biochemical Parameters ◽

Epstein Barr Virus ◽

Geographic Region ◽

Lymphoproliferative Disease ◽

Plasma Samples ◽

Genotype 1 ◽

Barr Virus ◽

Genotype 2 ◽

Epstein Barr

SummaryBackground: Primary Epstein-Barr virus (EBV) infection is usually asymptomatic, although at times it results in the benign lymphoproliferative disease, infectious mononucleosis (IM), during which almost half of patients develop hepatitis. The aims of the present study are to evaluate polymorphisms of EBV genes circulating in IM isolates from this geographic region and to investigate the correlation of viral sequence patterns with the available IM biochemical parameters.Methods: The study included plasma samples from 128 IM patients. The genes EBNA2, LMP1, and EBNA1 were amplified using nested-PCR. EBNA2 genotyping was performed by visualization of PCR products using gel electrophoresis. Investigation of LMP1 and EBNA1 included sequence, phylogenetic, and statistical analyses.Results: The presence of EBV DNA in plasma samples showed correlation with patients’ necessity for hospitalization (p=0.034). The majority of EBV isolates was genotype 1. LMP1 variability showed 4 known variants, and two new deletions (27-bp and 147-bp). Of the 3 analyzed attributes of LMP1 isolates, the number of 33-bp repeats less than the reference 4.5 was the only one that absolutely correlated with the elevated levels of transaminases. EBNA1 variability was presented by prototype subtypes. A particular combination of EBNA2, LMP1, and EBNA1 polymorphisms, deleted LMP1/P-thr and non-deleted LMP1/P-ala, as well as genotype 1/ 4.5 33-bp LMP1 repeats or genotype 2/ 4.5 33-bp LMP1 repeats showed correlation with elevated AST (aspartate aminotransferase) and ALT (alanine transaminase).Conclusions: This is the first study which identified the association between EBV variability and biochemical parameters in IM patients. These results showed a possibility for the identification of hepatic related diagnostic EBV markers.

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Rash in primary Epstein−Barr virus infection

Medicinski pregled ◽

10.2298/mpns1204138l ◽

2012 ◽

Vol 65 (3-4) ◽

pp. 138-141 ◽

Cited By ~ 1

Author(s):

Dajana Lendak ◽

Dunja Mihajlovic ◽

Vesna Turkulov ◽

Stefan Mikic ◽

Slavica Tomic

Keyword(s):

Virus Infection ◽

Infectious Mononucleosis ◽

Epstein Barr Virus ◽

Epstein Barr Virus Infection ◽

Duration Of Symptoms ◽

Barr Virus ◽

Significant Difference ◽

Epstein Barr ◽

Acute Infectious Mononucleosis ◽

Barr Virus Infection

Introduction. Rash can be one of the symptoms in acute infectious mononucleosis. According to the classical literature sources, drug-induced rash can be associated with synthetic penicillin and Epstein-Barr virus itself. Nowadays, a lot of case reports point to the development of rash after the administration of other groups of antimicrobials. Clinical and laboratory signs and the administration of antimicrobials in acute Epstein-Barr virus infection have been correlaed with the development of rash. Material and Methods. This retrospective-prospective study (2007-2010) included 243 patients hospitalized for acute infectious mononucleosis at the Department for Infectious Diseases, Clinical Center of Vojvodina, of whom 51 had rash and 192 were without it. Epstein-Barr virus infection was confirmed by ELISA IgM EBVVCA in all patients. Results. Student?s t-test did not show a significant difference between the age, gender, duration of symptoms, leucocytes count, absolute lymphocytes count, alanine aminotransferase, aspartate aminotransferase among patients with or without rash. ?2 test did not show a significant difference among the patients treated by synthetic or pure penicillin, macrolids and 1st and 2nd generation cephalosporins. However, if we compare all these antimicrobials, there is a significant difference between them and 3rd generation cephalosporins. Only two patients developed rash without antimicrobials. Conclusion. According to our results, rash developed independently of the clinical course of disease. Previous conclusion that synthetic penicillin produces rash seems to be not true, because there are many patients who were treated with them but did not develop rash. All antimicrobials can be associated with rash, but 3rd generation cephalosporins seem to produce rash less frequently than the others.

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