GENE EBNA3C: TYPES OF INFECTION BY EBV (EBV1 and EBV2), CORRELATION WITH CLINICAL AND BIOCHEMICAL PARAMETERS (AST, ALT and GGT) IN INDIVIDUALS WITH INFECTIOUS MONONUCLEOSIS OF THE METROPOLITAN AREA OF BELÉM, PARÁ, IN THE PERIOD OF 2005-2016

Metropolitan Area ◽

Biochemical Parameters ◽

Clinical Signs ◽

Metropolitan Region ◽

Significant Difference ◽

Clinical Cases

Abstract Two types of EBV (EBV1 and 2) have been shown to infect humans. Detect the types of EBV that cause infectious mononucleosis and correlate these viral types with biochemical parameters (AST, ALT and GGT) on in the metropolitan region of Belém, 2005 to 2016. A total 76 cases of infectious mononucleosis, processed the Instituto Evandro Chagas, Ananindeua, Brazil. Evaluated by PCR used primers the region EBNA 3C. EBV1 infection was observed in 71.1% (54/76) of individuals, among whom those > 14 years constituted 66.7% (36/54), the average age was 23 years, and the number of women infected was higher (61.1% [33/54]) than that of men (38.9%[21/54]). The symptoms/clinical signs observed in infection by EBV1 were cervical lymphadenopathy in 64.8% (35/54), fever in 63% (34/54), headache and arthralgia in 20.3% (11/54), and exanthema in 18.5% (10/54). Infection by EBV2 was observed in only 17.1% (13/76) of cases. Co-infection by EBV1 and EBV2, most frequently showing symptoms of fever and cervical lymphadenopathy, occurred in 66.7 (6/9) and 55.6% (5/9) of individuals. EBV1 was predominant in 71% of clinical cases of infectious mononucleosis. Correlation of biochemical parameters in infection by EBV1, EBV2, and co-infection by EBV1/2 revealed a statistically significant difference in mean changes of EBV1 in individuals older than 14 years.

Gene EBNA3C: Type of Infection by EBV (EBV1 and EBV2) Correlation With Clinical and Biochemical Parameters (AST, ALT and GGT) in Individuals With Infectious Mononucleosis in the Metropolitan Area of Belém, Pará, 2005-2016

10.21203/rs.2.10515/v3 ◽

2019 ◽

Author(s):

Talita Antonia Furtado Monteiro ◽

Iran Barros Costa ◽

Igor Brasil Costa ◽

Thais Letícia dos Santos Corrêa ◽

Beatriz Monteiro Rodrigues Coelho ◽

...

Keyword(s):

Social Science ◽

Metropolitan Area ◽

Biochemical Parameters ◽

Clinical Biochemistry ◽

Clinical Signs ◽

Metropolitan Region ◽

Significant Difference ◽

Biochemical Testing

Abstract Background: Two types of EBV (EBV1 and 2) have been shown to infect humans. This study aimed to detect the types of EBV that cause infectious mononucleosis and correlate these viral types with biochemical parameters (AST, ALT and GGT) in the metropolitan region of Belém from 2005 to 2016. Methods: A total of 76 cases of infectious mononucleosis were processed at the Instituto Evandro Chagas, Ananindeua, Brazil. PCR was used to analyze the EBNA3C region for the recognition of EBV types. Biochemical testing (AST, ALT and GGT) was performed by the COBAS INTEGRA clinical biochemistry PLUS 400 / ROCHE automatic analyzer. The data were evaluated using the Statistical Package for Social Science - SPSS 17.0 and GraphPadPrism 7.0 for Windows (GraphPad software, San Diego, CA, USA). Results: EBV1 infection was observed in 71.1% (54/76) of individuals, among whom those > 14 years constituted 66.7% (36/54); the average age was 23 years, and the number of women infected was higher (61.1% [33/54]) than that of men 38.9%[21/54]). The symptoms/clinical signs observed in infection by EBV1 were cervical lymphadenopathy in 64.8% (35/54), fever in 63% (34/54), headache and arthralgia in 20.3% (11/54), and exanthema in 18.5% (10/54). Infection by EBV2 was observed in only 17.1% (13/76) of cases. Coinfection by EBV1 and EBV2, most frequently showing symptoms of fever and cervical lymphadenopathy, occurred in 66.7% (6/9) and 55.6% (5/9) of individuals. Conclusion: EBV1 was predominant in 71% of clinical cases of infectious mononucleosis. The correlation of biochemical parameters in infection by EBV1, EBV2, and coinfection by EBV1/2 revealed a statistically significant difference in mean changes of EBV1 in individuals older than 14 years.

Gene EBNA3C: Type of Infection by EBV (EBV1 and EBV2) Correlation With Clinical and Biochemical Parameters (AST, ALT and GGT) in Individuals With Infectious Mononucleosis in the Metropolitan Area of Belém, Pará, 2005-2016

10.21203/rs.2.10515/v5 ◽

2019 ◽

Author(s):

Talita Antonia Furtado Monteiro ◽

Iran Barros Costa ◽

Igor Brasil Costa ◽

Thais Letícia dos Santos Corrêa ◽

Beatriz Monteiro Rodrigues Coelho ◽

...

Keyword(s):

Biochemical Parameters ◽

Clinical Signs ◽

Metropolitan Region ◽

Gamma Glutamyl Transferase ◽

Barr Virus ◽

Significant Difference ◽

Epstein Barr ◽

Glutamyl Transferase

Abstract Background: Two types of Epstein Barr virus (EBV1 and 2) have been shown to infect humans. This study aimed to detect the types of EBV that cause infectious mononucleosis and correlate these viral types with clinical parameters in the metropolitan region of Belém from 2005 to 2016.Methods: A total of 76 cases of infectious mononucleosis (IM) were processed at the Instituto Evandro Chagas, Ananindeua, Brazil. PCR was used to analyze the EBNA 3C region for the recognition of EBV types. Biochemical testing (aspartate aminotransferase (AST), alanine aminotransferase (ALT) and gamma-glutamyl transferase (GGT) was performed by the COBAS INTEGRA clinical biochemistry PLUS 400 / ROCHE automatic analyzer. The data were evaluated using the Statistical Package for Social Science - SPSS 17.0 and GraphPadPrism 7.0 for Windows.Results: EBV1 infection was observed in 71.1% (54/76) of individuals, among whom those > 14 years constituted 66.7% (36/54); the average age was 23 years, and the number of women infected was higher (61.1% [33/54]) than that of men 38.9%[21/54]). The symptoms/clinical signs observed in infection by EBV1 were cervical lymphadenopathy in 64.8% (35/54), fever in 63% (34/54), headache and arthralgia in 20.3% (11/54), and exanthema in 18.5% (10/54). Infection by EBV2 was observed in only 17.1% (13/76) of cases. Coinfections by EBV1 and EBV2, most frequently showing symptoms of fever and cervical lymphadenopathy, occurred in 66.7% (6/9) and 55.6% (5/9) of individuals. Alterations to AST were confirmed in 14.8% (8/54), EBV2 of cases in 7.7% (1/13) in EBV1 infection. Conclusions: EBV1 was predominant in 71% of clinical cases of infectious mononucleosis. The correlation of biochemical parameters in infection by EBV1, EBV2, and coinfections by EBV1/2 revealed a statistically significant difference in mean changes of EBV1 in individuals older than 14 years.

Gene EBNA3C: Type of Infection by EBV (EBV1 and EBV2) Correlation With Clinical and Biochemical Parameters (AST, ALT and GGT) in Individuals With Infectious Mononucleosis in the Metropolitan Area of Belém, Pará, 2005-2016

10.21203/rs.2.10515/v4 ◽

2019 ◽

Author(s):

Talita Antonia Furtado Monteiro ◽

Iran Barros Costa ◽

Igor Brasil Costa ◽

Thais Letícia dos Santos Corrêa ◽

Beatriz Monteiro Rodrigues Coelho ◽

...

Keyword(s):

Biochemical Parameters ◽

Clinical Signs ◽

Metropolitan Region ◽

Gamma Glutamyl Transferase ◽

Barr Virus ◽

Significant Difference ◽

Epstein Barr ◽

Glutamyl Transferase

Abstract Background: Two types of Epstein Barr virus (EBV1 and 2) have been shown to infect humans. This study aimed to detect the types of EBV that cause infectious mononucleosis and correlate these viral types with clinical parameters in the metropolitan region of Belém from 2005 to 2016.Methods: A total of 76 cases of infectious mononucleosis (IM) were processed at the Instituto Evandro Chagas, Ananindeua, Brazil. PCR was used to analyze the EBNA 3C region for the recognition of EBV types. Biochemical testing (aspartate aminotransferase (AST), alanine aminotransferase (ALT) and gamma-glutamyl transferase (GGT) was performed by the COBAS INTEGRA clinical biochemistry PLUS 400 / ROCHE automatic analyzer. The data were evaluated using the Statistical Package for Social Science - SPSS 17.0 and GraphPadPrism 7.0 for Windows.Results: EBV1 infection was observed in 71.1% (54/76) of individuals, among whom those > 14 years constituted 66.7% (36/54); the average age was 23 years, and the number of women infected was higher (61.1% [33/54]) than that of men 38.9%[21/54]). The symptoms/clinical signs observed in infection by EBV1 were cervical lymphadenopathy in 64.8% (35/54), fever in 63% (34/54), headache and arthralgia in 20.3% (11/54), and exanthema in 18.5% (10/54). Infection by EBV2 was observed in only 17.1% (13/76) of cases. Coinfections by EBV1 and EBV2, most frequently showing symptoms of fever and cervical lymphadenopathy, occurred in 66.7% (6/9) and 55.6% (5/9) of individuals. Alterations to AST were confirmed in 14.8% (8/54), EBV2 of cases in 7.7% (1/13) in EBV1 infection. Conclusions: EBV1 was predominant in 71% of clinical cases of infectious mononucleosis. The correlation of biochemical parameters in infection by EBV1, EBV2, and coinfections by EBV1/2 revealed a statistically significant difference in mean changes of EBV1 in individuals older than 14 years.

Epstein Barr virus genotypes (EBV1/EBV2) in individuals with infectious mononucleosis in the metropolitan area of Belém, Brazil, between 2005 and 2016

10.21203/rs.3.rs-16300/v1 ◽

2020 ◽

Author(s):

Talita Antonia Furtado Monteiro ◽

Iran Barros Costa ◽

Igor Brasil Costa ◽

Thais Letícia dos Santos Corrêa ◽

...

Keyword(s):

Epstein Barr Virus ◽

Health Care Service ◽

Clinical Signs ◽

Metropolitan Region ◽

Gamma Glutamyl Transferase ◽

Barr Virus ◽

Epstein Barr ◽

Glutamyl Transferase

Abstract Background: Two types of Epstein Barr virus (EBV1/EBV2) have been shown to infect humans by causing infectious mononucleosis, their genome being very similar, except for regions of the EBNA genes. This study aimed to describe the EBV genotypes in cases of infectious mononucleosis in the metropolitan region of Belém, Brazil, from 2005 to 2016. Methods: A total of 8.295 suspected cases with symptoms/signs of infectious mononucleosis (MI) were investigated by doctors of infectious diseases at the Evandro Chagas Institute Health Care Service from January 2005 to December 2016. In 3.0% (251/8.295) of the cases were positive by enzymatic immunoassay were submitted to PCR for EBNA3C region to detect the types of EBV. Biochemical testing involving aspartate aminotransferase, alanine aminotransferase and gamma-glutamyl transferase were realized. Results: The identification of EBV types by PCR was verified in 30.3% (76/251) of individuals, being 71.1% (54/76) classified as EBV1, 17.1% (13/76) as EBV2 and 11.8% (9/76) as EBV1+EBV2. The number of women infected with EBV1 was higher (61.1% - 33/54) than for men (38.9% - 21/54), most were over 14 years old (66.7%-36/54). The main symptoms/clinical signs observed in EBV1 infection were: cervical lymphadenopathy (64.8%-35/54), fever (63%-34/54), headache (20.3%-11/54), arthralgia (20.3%-11/54) and exanthema (18.5%-10/54). In EBV2 infection, it was also detected in all age groups, with the exception of two groups, with an average age of 24 years. The presence of fever in 76.9% (10/13) with an average duration of 18 days and lymphadenopathy in 53.8 (7/13) were the most relevant signs/symptoms in EBV2. In contrast, EBV1+EBV2 co-infection was more frequent in the £5 year age group, affecting 20.0% (2/10). Women presented 66.7% (6/9) more positive cases. The symptoms involving EBV1+EBV2 co-infection were more related to fever (66.7%-6/9) and cervical lymphadenopathy (55.6% -5/9). The average of enzymatic values according to type of EBV was statistically significant (p <0.05) in individuals with EBV1 infection in those over 14 years of age. Conclusions: A pioneering study that molecularly identified the genotypes of EBV in 30.3% of cases, with circulation of EBV1, EBV2 and co-infection EBV1+EBV2 in cases of infectious mononucleosis in the northern region of Brazil.

ACUTE TOXICITY EVALUATION OF ETHANOLIC EXTRACT OF ARISTOLOCHIA ALBIDA DUCH. LEAVES ON WISTAR RATS LIVER AND KIDNEY FUNCTIONS

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i7.16887 ◽

2017 ◽

Vol 9 (7) ◽

pp. 35 ◽

Cited By ~ 1

Author(s):

Felix F. D. Guinnin ◽

Jean Robert Klotoe ◽

Jean Marc Ategbo

Keyword(s):

Biochemical Parameters ◽

Statistical Difference ◽

Clinical Signs ◽

Ethanolic Extract ◽

Albino Rats ◽

Acute Oral Toxicity ◽

Oral Toxicity ◽

Mean Cell Volume ◽

Limit Test ◽

Significant Difference

Objective: Aristolochia albida Duch is frequently used by alternative medicine to treat some pathologies like hepatitis. Our main objective was to evaluate the acute oral toxicity of the ethanolic extract of this plant.Methods: Exploratory tests for acute oral toxicity are performed in vivo on Wistar albino rats in a limit test of 2000 mg/kg for 14 d in accordance with the OECD Guidelines 423. The clinical signs were observed every day, followed by measurement of body weight change, the haematological and biochemical examinations were executed and statistical analysis was performed. Results: The various clinical signs observed after administration and for 14 d were recorded and no mortality was observed. With the exception of white blood cells, mean cell volume and platelets with statistically significant difference in control (p<0.05), all the haematological parameters showed an insignificant statistical difference to the control ratio (p>0.05). For biochemical parameters, except blood glucose and total protein of control batches, which show a significant statistical difference after 14 d (p>0.05), all the biochemical parameters show the statistically insignificant difference for test and controls batches (p>0.05) as well as the weight variation of the animals.Conclusion: The ethanolic extract of the leaves of A. albida Duch (EEAr) had not toxic effect on the biochemical and hematological parameters studied at a dose of 2000 mg/kg. The lethal dose is therefore over 2000 mg/kg.

Clinical and molecular diagnosis of Chlamydophila in captive parrots in Pará State, Brazil

Semina Ciências Agrárias ◽

10.5433/1679-0359.2019v40n6p2603 ◽

2019 ◽

Vol 40 (6) ◽

pp. 2603

Author(s):

Simone Aparecida Almeida Araujo ◽

Washington Luiz Assunção Pereira ◽

Sandro Patroca da Silva ◽

Jedson Ferreira Cardoso ◽

Ednaldo da Silva Filho ◽

...

Keyword(s):

Metropolitan Area ◽

Clinical Signs ◽

Infectious Agent ◽

Chi Square ◽

Breeding Sites ◽

Incidence Data ◽

Significant Difference ◽

Species Specific ◽

Conservation Breeding ◽

Globally Distributed

Parrots (Order Psittaciformes) are globally distributed birds that, together with members of the Columbiformes, represent the most susceptible animals, in regards to infection by Chlamydophila psittaci, which is an obligate, zoonotic, intracellular bacterium that causes chlamydiosis in domestic and wild birds and psittacosis in humans. The aim of the present study was to assess the occurrence of C. psittaci in Brazilian psittacids kept in conservation breeding sites in Pará State, Brazil. Cloacal and oropharynx swab samples were collected from 201 psittacids that were distributed among four breeding sites: Metropolitan Area of Belém (C1 and C2), Northeastern Pará (C3), and Low Amazon (C4). The samples were screened for C. psittaci using semi-nested PCR, and the resulting incidence data were analyzed using proportion and chi-square tests. Chlamydophila infection was confirmed for all the breeding sites, with an overall prevalence of 31.84%, and no species-specific predisposition was observed. Furthermore, 13.93% of the sampled birds eliminated the infectious agent by the cloaca, whereas 11.44% eliminated the agent by the oropharynx, and 6.47% eliminated the agent by both routes. Moreover, there was a significant difference between the incidence of Chlamydophila infection of breeding sites C2 and C3 (p=0.029), which yielded the smallest and largest number of diagnosed cases, respectively. In the present study, most of the birds (27.86%) were considered unapparent carriers of Chlamydophila infection, and only 3.98% of the birds yielded both a positive diagnosis and clinical signs of chlamydiosis.

The cerebrospinal fluid changes and clinical characteristics of aseptic meningitis in Kawasaki disease

10.21203/rs.3.rs-16533/v1 ◽

2020 ◽

Author(s):

Fan Hu ◽

Xiaoqing Shi ◽

Yang Fan ◽

Hanmin Liu ◽

Kaiyu Zhou

Keyword(s):

Cerebrospinal Fluid ◽

Kawasaki Disease ◽

Aseptic Meningitis ◽

Clinical Symptoms ◽

Clinical Signs ◽

C Reactive Protein ◽

Neck Stiffness ◽

Reactive Protein ◽

Significant Difference

Abstract Background: Aseptic meningitis is not a common feature in Kawasaki disease (KD). However, it could cause difficulty in making correct and in-time diagnosis Methods: We retrospectively investigated patients of KD and bacterial meningitis (BM). Totally 38 KD patients and 126 BM patients were brought into this study. Clinical symptoms, signs and laboratory examinations were compared between the two groups, which included: duration of fever before lumbar puncture, conjunctiva injection, oral cavity change, rash, cervical lymphadenopathy and extremities change, vomiting, front fontanel bulging, neck stiffness, leukocytes, hemoglobin, platelets, C-reactive protein, cerebrospinal fluid examinations, liver function and urinalysis. Results: In clinical signs, vomit and neck stiffness were more prevalent in BM. KD patients showed higher blood leukocyte (p<0.001) and C-reactive protein (p<0.001) in the early febrile stage. Glucose in cerebrospinal fluid of BM patients was significantly lower than KD patients (p=0.003). In ROC curve, the optimal cutoff value of CSF glucose was 2.945mmol/L with the sensitivity of 84.2% and specificity of 71.4%. Pyuria was more prevalent in KD patients (p<0.001). There was no significant difference in front fontanel bulging, hemoglobin, platelet, alanine transaminase, aspartate transaminase, albumin, cerebrospinal fluid leukocytes, cerebrospinal fluid protein and cerebrospinal fluid lactate dehydrogenase. Conclusions ： Full investigation of clinical manifestation and laboratory tests is necessary to distinguish KD with aseptic meningitis and BM. In CSF study, glucose level is more efficient than other items to distinguish these two diseases. Decreased CSF glucose is possibly an indicator of BM rather than KD.

In-vivo anti-oxidant screening of Tectona grandis extract

International Research Journal of Pharmaceutical and Applied Sciences ◽

10.26452/irjpas.v9i1.1164 ◽

2020 ◽

Vol 9 (1) ◽

pp. 1-4

Author(s):

Tamilarasi G P ◽

Sabarees G

Keyword(s):

Biochemical Parameters ◽

Tectona Grandis ◽

The Body ◽

Altered Expression ◽

Physiological Conditions ◽

Synthetic Drugs ◽

Significant Difference ◽

Anti Oxidant ◽

Radical Generation

Oxidation is an essential reaction in the human body, which determines the expression of proteins in the body. This results in the altered expression like rapid growth resulting in cancers and other disorders. Many synthetic drugs are available in the market that is effective in limiting the free radical generation and the reaction of radicals with cells. Unfortunately, all those synthetic drugs were found to cause side effects and adverse effects in the body. But given the accuracy of the predictability of the results and administration, this research focuses on testing the anti-oxidant efficiency in rat models testing the biochemical parameters. Investigations have also been done on the anti-oxidant activity of Tectona, but every research was concentrated to prove the anti-oxidant activity only. extract had been tested for anti-oxidant activity by estimating various tissue parameters and it showed better activity. As predicted, there is a significant difference in the and results which can be explained are due to the physiological conditions that exist inside the body.

Comparison of the Effects of Fenugreek Vaginal Cream and Ultra Low- Dose Estrogen on Atrophic Vaginitis

Current Drug Delivery ◽

10.2174/1567201817666200708112655 ◽

2020 ◽

Vol 17 (9) ◽

pp. 815-822

Author(s):

Maryam Safary ◽

Sevil Hakimi ◽

Noushin Mobaraki-Asl ◽

Paria Amiri ◽

Habib Tvassoli ◽

...

Keyword(s):

Postmenopausal Women ◽

Low Dose ◽

Intervention Group ◽

Clinical Signs ◽

Control Group ◽

Conjugated Estrogens ◽

Atrophic Vaginitis ◽

Vaginal Cream ◽

Intergroup Comparison ◽

Significant Difference

Introduction: Atrophic vaginitis is a common problem in postmenopausal women and results from decreased levels of blood estrogen. It is associated with symptoms of itching, burning, dyspareunia, and postmenopausal bleeding. The present study evaluated the effects of fenugreek extract on atrophic vaginitis. Materials and Methods: This randomized controlled clinical trial was performed on 60 postmenopausal women in Ardabil, Iran, in 2018. The participants were selected using block randomization with the allocation ratio 1:1. Those in the intervention group received 0.5g (the applicator filled to the half-full mark) fenugreek vaginal cream 5% twice a week for 12 weeks. The control group received conjugated estrogens vaginal cream at the dose of 0.625 mg (the applicator filled to the half-full mark) containing 0.3 mg of conjugated estrogens. Atrophic vaginitis was evaluated before and after the treatment through clinical examination, clinical signs, and measurement of Vaginal Maturation Index (VMI). Findings: After the 12-week intervention and modification of the baseline score, the mean (standard error) score for atrophic vaginitis signs was 3.100 (1.43-4.75). This difference was statistically significant in intragroup comparison and in favor of the control group in intergroup comparison (p=0.001). VMI was less than 49% in 86.7% and 46.7% of the participants in the intervention and control groups, respectively. This was a significant difference in favor of the control group (p=0.001). Conclusion: The results of this study showed that total fenugreek extract could be effective in treating signs of atrophic vaginitis, but it was not as effective as ultra-low-dose estrogen.