scholarly journals Phytochemical and comparative transcriptome analyses reveal different regulatory mechanisms in the terpenoid biosynthesis pathways between Matricaria recutita L. and Chamaemelum nobile L.

2020 ◽  
Author(s):  
yuling tai ◽  
Xiaojuan Hou ◽  
Chun Liu ◽  
Jiameng Sun ◽  
Chunxiao Guo ◽  
...  
Keyword(s):  
Essential Oils ◽  
Rapid Evolution ◽  
Single Copy ◽  
Essential Oil Composition ◽  
Regulatory Mechanisms ◽  
Terpenoid Biosynthesis ◽  
Cytochrome P450 Enzymes ◽  
Oil Composition ◽  
Matricaria Recutita ◽  
Chamaemelum Nobile

Abstract Background Matricaria recutita (German chamomile) and Chamaemelum nobile (Roman chamomile) belong to the botanical family Asteraceae. These two herbs are not only morphologically distinguishable, but their secondary metabolites – especially the essential oils present in flowers are also different, especially the terpenoids. The aim of this project was to preliminarily identify regulatory mechanisms in the terpenoid biosynthetic pathways that differ between German and Roman chamomile by performing comparative transcriptomic and metabolomic analyses. Results We determined the content of essential oils in disk florets and ray florets in these two chamomile species, and found that the terpenoid content in flowers of German chamomile is greater than that of Roman chamomile. In addition, a comparative RNA-seq analysis of German and Roman chamomile showed that 54% of genes shared >75% sequence identity between the two species. In particular, more highly expressed DEGs (differentially expressed genes) and TF (transcription factor) genes, different regulation of CYPs (cytochrome P450 enzymes), and rapid evolution of downstream genes in the terpenoid biosynthetic pathway of German chamomile could be the main reasons to explain the differences in the types and levels of terpenoid compounds in these two species. In addition, a phylogenetic tree constructed from single copy genes showed that German chamomile and Roman chamomile are closely related to Chrysanthemum nankingense. Conclusion This work provides the first insights into terpenoid biosynthesis in two species of chamomile. The candidate unigenes related to terpenoid biosynthesis will be important in molecular breeding approaches to modulate the essential oil composition of Matricaria recutita and Chamaemelum nobile.

scholarly journals Phytochemical and comparative transcriptome analyses reveal different regulatory mechanisms in the terpenoid biosynthesis pathways between Matricaria recutita L. and Chamaemelum nobile L.

2020 ◽  
Author(s):  
yuling tai ◽  
Xiaojuan Hou ◽  
Chun Liu ◽  
Jiameng Sun ◽  
Chunxiao Guo ◽  
...  
Keyword(s):  
Essential Oils ◽  
Rapid Evolution ◽  
Single Copy ◽  
Essential Oil Composition ◽  
Regulatory Mechanisms ◽  
Terpenoid Biosynthesis ◽  
Cytochrome P450 Enzymes ◽  
Oil Composition ◽  
Matricaria Recutita ◽  
Chamaemelum Nobile

Abstract Background Matricaria recutita (German chamomile) and Chamaemelum nobile (Roman chamomile) belong to the botanical family Asteraceae. These two herbs are not only morphologically distinguishable, but their secondary metabolites – especially the essential oils present in flowers are also different, especially the terpenoids. The aim of this project was to preliminarily identify regulatory mechanisms in the terpenoid biosynthetic pathways that differ between German and Roman chamomile by performing comparative transcriptomic and metabolomic analyses. Results We determined the content of essential oils in disk florets and ray florets in these two chamomile species, and found that the terpenoid content in flowers of German chamomile is greater than that of Roman chamomile. In addition, a comparative RNA-seq analysis of German and Roman chamomile showed that 54% of genes shared >75% sequence identity between the two species. In particular, more highly expressed DEGs (differentially expressed genes) and TF (transcription factor) genes, different regulation of CYPs (cytochrome P450 enzymes), and rapid evolution of downstream genes in the terpenoid biosynthetic pathway of German chamomile could be the main reasons to explain the differences in the types and levels of terpenoid compounds in these two species. In addition, a phylogenetic tree constructed from single copy genes showed that German chamomile and Roman chamomile are closely related to Chrysanthemum nankingense. Conclusion This work provides the first insights into terpenoid biosynthesis in two species of chamomile. The candidate unigenes related to terpenoid biosynthesis will be important in molecular breeding approaches to modulate the essential oil composition of Matricaria recutita and Chamaemelum nobile.

scholarly journals Phytochemical and comparative transcriptome analyses reveal different regulatory mechanisms in the terpenoid biosynthesis pathways between Matricaria recutita L. and Chamaemelum nobile L.

2020 ◽  
Author(s):  
yuling tai ◽  
Xiaojuan Hou ◽  
Chun Liu ◽  
Jiameng Sun ◽  
Chunxiao Guo ◽  
...  
Keyword(s):  
Essential Oils ◽  
Rapid Evolution ◽  
Single Copy ◽  
Essential Oil Composition ◽  
Regulatory Mechanisms ◽  
Terpenoid Biosynthesis ◽  
Cytochrome P450 Enzymes ◽  
Oil Composition ◽  
Matricaria Recutita ◽  
Chamaemelum Nobile

Abstract Background Matricaria recutita (German chamomile) and Chamaemelum nobile (Roman chamomile) belong to the botanical family Asteraceae. These two herbs are not only morphologically distinguishable, but their secondary metabolites – especially the essential oils present in flowers are also different, especially the terpenoids. The aim of this project was to preliminarily identify regulatory mechanisms in the terpenoid biosynthetic pathways that differ between German and Roman chamomile by performing comparative transcriptomic and metabolomic analyses. Results We determined the content of essential oils in disk florets and ray florets in these two chamomile species, and found that the terpenoid content in flowers of German chamomile is greater than that of Roman chamomile. In addition, a comparative RNA-seq analysis of German and Roman chamomile showed that 54% of genes shared >75% sequence identity between the two species. In particular, more highly expressed DEGs (differentially expressed genes) and TF (transcription factor) genes, different regulation of CYPs (cytochrome P450 enzymes), and rapid evolution of downstream genes in the terpenoid biosynthetic pathway of German chamomile could be the main reasons to explain the differences in the types and levels of terpenoid compounds in these two species. In addition, a phylogenetic tree constructed from single copy genes showed that German chamomile and Roman chamomile are closely related to Chrysanthemum nankingense. Conclusion This work provides the first insights into terpenoid biosynthesis in two species of chamomile. The candidate unigenes related to terpenoid biosynthesis will be important in molecular breeding approaches to modulate the essential oil composition of Matricaria recutita and Chamaemelum nobile.

scholarly journals Phytochemical and comparative transcriptome analyses reveal different regulatory mechanisms in the terpenoid biosynthesis pathways between Matricaria recutita L. and Chamaemelum nobile L.

2020 ◽  
Author(s):  
yuling tai(Former Corresponding Author) ◽  
Xiaojuan Hou ◽  
Chun Liu ◽  
Jiameng Sun ◽  
Chunxiao Guo ◽  
...  
Keyword(s):  
Essential Oils ◽  
Rapid Evolution ◽  
Single Copy ◽  
Essential Oil Composition ◽  
Regulatory Mechanisms ◽  
Terpenoid Biosynthesis ◽  
Cytochrome P450 Enzymes ◽  
Oil Composition ◽  
Matricaria Recutita ◽  
Chamaemelum Nobile

Abstract Background: Matricaria recutita (German chamomile) and Chamaemelum nobile (Roman chamomile) belong to the botanical family Asteraceae. These two herbs are not only morphologically distinguishable, but their secondary metabolites – especially the essential oils present in flowers are also different, especially the terpenoids. The aim of this project was to preliminarily identify regulatory mechanisms in the terpenoid biosynthetic pathways that differ between German and Roman chamomile by performing comparative transcriptomic and metabolomic analyses. Results: We determined the content of essential oils in disk florets and ray florets in these two chamomile species, and found that the terpenoid content in flowers of German chamomile is greater than that of Roman chamomile. In addition, a comparative RNA-seq analysis of German and Roman chamomile showed that 54% of genes shared >75% sequence identity between the two species. In particular, more highly expressed DEGs (differentially expressed genes) and TF (transcription factor) genes, different regulation of CYPs (cytochrome P450 enzymes), and rapid evolution of downstream genes in the terpenoid biosynthetic pathway of German chamomile could be the main reasons to explain the differences in the types and levels of terpenoid compounds in these two species. In addition, a phylogenetic tree constructed from single copy genes showed that German chamomile and Roman chamomile are closely related to Chrysanthemum nankingense.Conclusion: This work provides the first insights into terpenoid biosynthesis in two species of chamomile. The candidate unigenes related to terpenoid biosynthesis will be important in molecular breeding approaches to modulate the essential oil composition of Matricaria recutita and Chamaemelum nobile.

scholarly journals Phytochemical and comparative transcriptome analyses reveal different regulatory mechanisms in the terpenoid biosynthesis pathways between Matricaria recutita L. and Chamaemelum nobile L.

2020 ◽  
Author(s):  
Yuling Tai ◽  
Xiaojuan Hou ◽  
Chun Liu ◽  
Jiameng Sun ◽  
Chunxiao Guo ◽  
...  
Keyword(s):  
Essential Oils ◽  
Rapid Evolution ◽  
Single Copy ◽  
Essential Oil Composition ◽  
Regulatory Mechanisms ◽  
Terpenoid Biosynthesis ◽  
Cytochrome P450 Enzymes ◽  
Oil Composition ◽  
Matricaria Recutita ◽  
Chamaemelum Nobile

Abstract Background Matricaria recutita (German chamomile) and Chamaemelum nobile (Roman chamomile) belong to the botanical family Asteraceae. These two herbs are not only morphologically distinguishable, but their secondary metabolites – especially the essential oils present in flowers are also different, especially the terpenoids. The aim of this project was to preliminarily identify regulatory mechanisms in the terpenoid biosynthetic pathways that differ between German and Roman chamomile by performing comparative transcriptomic and metabolomic analyses. Results We determined the content of essential oils in disk florets and ray florets in these two chamomile species, and found that the terpenoid content in flowers of German chamomile is greater than that of Roman chamomile. In addition, a comparative RNA-seq analysis of German and Roman chamomile showed that 54% of genes shared >75% sequence identity between the two species. In particular, more highly expressed DEGs (differentially expressed genes) and TF (transcription factor) genes, different regulation of CYPs (cytochrome P450 enzymes), and rapid evolution of downstream genes in the terpenoid biosynthetic pathway of German chamomile could be the main reasons to explain the differences in the types and levels of terpenoid compounds in these two species. In addition, a phylogenetic tree constructed from single copy genes showed that German chamomile and Roman chamomile are closely related to Chrysanthemum nankingense. Conclusion This work provides the first insights into terpenoid biosynthesis in two species of chamomile. The candidate unigenes related to terpenoid biosynthesis will be important in molecular breeding approaches to modulate the essential oil composition of Matricaria recutita and Chamaemelum nobile.

scholarly journals Phytochemical and comparative transcriptome analysis reveals different regulatory mechanisms in terpenoids pathways between Matricaria recutita L. and Chamaemelum nobile L

2019 ◽  
Author(s):  
Yuling Tai ◽  
Xiaojuan Hou ◽  
Chun Liu ◽  
Jiameng Sun ◽  
Chunxiao Guo ◽  
...  
Keyword(s):  
Essential Oil ◽  
Rapid Evolution ◽  
Essential Oil Composition ◽  
Regulatory Mechanisms ◽  
Rna Seq ◽  
Cytochrome P450 Enzymes ◽  
Oil Composition ◽  
High Identity ◽  
Matricaria Recutita ◽  
Chamaemelum Nobile

Abstract Background Matricaria recutita (German chamomile) and Chamaemelum nobile (Roman chamomile) belong to the botanical family Asteraceae. These two herbs are not only morphologically distinguishable, but their secondary metabolites – especially the essential oils in flowers are also different, especially the terpenoids. The aim of this project was to preliminarily reveal differential regulatory mechanisms in terpenoids biosynthetic pathway between German and Roman chamomile by performing comparative transcriptomic and metabolomics analyses. Results We determined the content of essential oil in disk florets and ray florets in these two chamomiles, and found that the terpenoids content of German chamomile is greater than that of Roman chamomile. In addition, comparisons between German and Roman chamomile were studied by RNA-Seq, which showed that 54% of genes shared >75% sequence identity between the two species. In particular, more highly expressed DEGs (differentially expressed genes) and TFs (transcription factors), different regulation of CYPs (cytochrome P450 enzymes) and rapid evolution of the downstream enzymes in terpenoids biosynthetic pathways in German chamomile, and maybe the reasons result to differences in the types and levels of terpenoids compounds in these two chamomiles. In addition, the phylogenetic tree of single copay genes showed that German chamomile and Roman chamomile had a high identity to C. nankingense. Conclusion This work provided first insights into the terpenoids biosynthesis of chamomiles, and the candidate unigenes related to terpenoids biosynthesis can serve as important information for molecular breeding approaches to modulate the essential oil composition.

Review of the Essential Oil Composition of Iranian Endemic and Native Taxa of Apiaceae (Umbelliferae)

2020 ◽  
Vol 24 (9) ◽  
pp. 909-1009
Author(s):  
Maryam Akaberi ◽  
Zahra Tayarani-Najaran ◽  
Iraj Mehregan ◽  
Javad Asili ◽  
Amirhossein Sahebkar ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Essential Oil Composition ◽  
Present Review ◽  
Aromatic Plants ◽  
Oil Composition

One of the most important families of Iranian flora is Apiaceae (Umbelliferae). Most of the species of this family are aromatic plants and rich in essential oils with diverse structures. In the present review, the essential oil composition of 63 genera comprising 141 Apiaceae (66.4% native 33.6% endemic) is summarized.

Evaluation of antioxidant and antimicrobial activity of Ziziphora ‎clinopodiodes essential oil eluted at different times

2020 ◽  
Vol 16 ◽  
Author(s):  
Mojgan Alizadeh ◽  
Akram Arianfar ◽  
Ameneh Mohammadi
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Antioxidant Activities ◽  
Positive Control ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Citronellic Acid ◽  
Antimicrobial And Antioxidant Activity

Objective: Ziziphora clinopodioides is an edible medicinal plant belongs to the Labiatae family that widespread all over Iran. It used as culinary and also in cold and cough treatments in Iran. The aim of present work was to evaluate the effect of different timeframes during the hydrodistillation on essential oil composition, antimicrobial and antioxidant activity. Materials and Methods: The essential oil of Z. clinopodiodes was extracted via hydrodistillation with Clevenger apparatus. The fractions of essential oil were captured at 6 times from the beginning of the distillation: (10, 20, 60, 120, 180 and 240 min). The fractions of essential oil were analyzed by GC/MS and their antibacterial, antifungal and antioxidant activities were studied by Disk - well diffusion and DPPH methods respectively. Results: Six distillation times and whole essential oil were captured during the hydrodistillation. Essential oil yield dropped off significantly during distillation progressed (1.0% for 10 min and 0.025 for 240 min). 1,8 Cineol, Isomenthone, Pulegone, Piperitenone and Citronellic acid were major compounds in fractions and they were affected by distillation times. Pulegone was major compound in all of essential oils. In antioxidant activity assay, whole essential oil was stronger than was stronger than positive control and fractions of essential oil, because of higher levels of Isomenthone, Piperitenone and Citronellic acid. Strongest antimicrobial activity against S. aureus, E. coli and C. albicans was observed from 10 min fraction. Conclusion: Our results indicated that distillation time can create essential oils with specific properties and we can achieve to more efficient essential oil in short times.

scholarly journals Compositional Analysis and Aroma Evaluation of Feijoa Essential Oils from New Zealand Grown Cultivars

Molecules ◽  
2019 ◽  
Vol 24 (11) ◽  
pp. 2053 ◽  
Author(s):  
Yaoyao Peng ◽  
Karen Suzanne Bishop ◽  
Siew Young Quek
Keyword(s):  
New Zealand ◽  
Essential Oil ◽  
Essential Oils ◽  
Extraction Method ◽  
Compositional Analysis ◽  
Extraction Methods ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Active Compounds ◽  
Initial Comparison

Feijoa is an aromatic fruit and the essential oil from feijoa peel could be a valuable by-product in the juicing industry. An initial comparison of the essential oil extraction methods, steam-distillation and hydro-distillation, was conducted. The volatile compounds in the essential oils from four feijoa cultivars were identified and semi-quantified by GC-MS and the aroma active compounds in each essential oil were characterized using SPME-GC-O-MS. Hydro-distillation, with a material to water ratio of 1:4 and an extraction time of 90 min, was the optimized extraction method for feijoa essential oil. The Wiki Tu cultivar produced the highest essential oil yield among the four selected cultivars. A total of 160 compounds were detected, among which 90 compounds were reported for the first time in feijoa essential oils. Terpenes and esters were dominant compounds in feijoa essential oil composition and were also major contributors to feijoa essential oil aroma. Key aroma active compounds in feijoa essential oils were α-terpineol, ethyl benzoate, (Z)-3-hexenyl hexanoate, linalool, (E)-geraniol, 2-undecanone, 3-octanone, α-cubebene, and germacrene D. This is the first report on the optimization of the extraction method and the establishment of the aroma profile of feijoa essential oils, with a comparison of four New Zealand grown cultivars.

scholarly journals Essential Oil Composition ofPinus peuceGriseb. Needles and Twigs from Two National Parks of Kosovo

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Avni Hajdari ◽  
Behxhet Mustafa ◽  
Dashnor Nebija ◽  
Hyrmete Selimi ◽  
Zeqir Veselaj ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
National Park ◽  
Essential Oil Composition ◽  
Gas Chromatography Mass Spectrometry ◽  
Oil Composition ◽  
Plant Materials ◽  
Geographical Variations

The principal aim of this study was to analyze the chemical composition and qualitative and quantitative variability of essential oils obtained from seven naturally grown populations of thePinus peuceGrisebach, Pinaceae in Kosovo. Plant materials were collected from three populations in the Sharri National Park and from four other populations in the Bjeshkët e Nemuna National Park, in Kosovo. Essential oils were obtained by steam distillation and analyzed by GC-FID (Gas Chromatography-Flame Ionization Detection) and GC-MS (Gas Chromatography-Mass Spectrometry). The results showed that the yield of essential oils (v/wdry weight) varied depending on the origin of population and the plant organs and ranged from 0.7 to 3.3%. In total, 51 compounds were identified. The main compounds wereα-pinene (needles: 21.6–34.9%; twigs: 11.0–24%),β-phellandrene (needles: 4.1–27.7; twigs: 29.0–49.8%), andβ-pinene (needles: 10.0–16.1; twigs: 6.9–20.7%). HCA (Hierarchical Cluster Analysis) and PCA (Principal Component Analyses) were used to assess geographical variations in essential oil composition. Statistical analysis showed that the analyzed populations are grouped in three main clusters which seem to reflect microclimatic conditions on the chemical composition of the essential oils.

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