scholarly journals Functional analysis of GhCHS, GhANR and GhLAR in colored fiber formation of Gossypium hirsutum L.

2019 ◽  
Author(s):  
Gao Jianfang ◽  
Shen Li ◽  
Yuan Jingli ◽  
Zheng Hongli ◽  
Su Quansheng ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Cotton Fiber ◽  
Fiber Formation ◽  
Gossypium Hirsutum L ◽  
Expression Levels ◽  
Color Formation ◽  
Colored Cotton ◽  
Natural Colored Cotton ◽  
Key Genes ◽  
Color Depth

Abstract Background The formation of natural colored fibers mainly results from the accumulation of different anthocyanidins and their derivatives in the fibers of Gossypium hirsutum L. Chalcone synthase (CHS) is the first committed enzyme of flavonoid biosynthesis, and anthocyanidins are transported into fiber cell after biosynthesis mainly by Anthocyanidin reductase (ANR) and Leucoanthocyanidin reductase (LAR) to present diverse colors with distinct stability. The biochemical and molecular mechanism of pigment formation in natural colored cotton fiber is not clear. Results The three key genes of GhCHS , GhANR and GhLAR were predominantly expressed in the developing fibers of colored cotton. In the GhCHSi , GhANRi and GhLARi transgenic cottons, the expression levels of GhCHS , GhANR and GhLAR significantly decreased in the developing cotton fiber, negatively correlated with the content of anthocyanidins and the color depth of cotton fiber. In colored cotton Zongxu1 (ZX1) and the GhCHSi , GhANRi and GhLARi transgenic lines of ZX1, HZ and ZH, the anthocyanidin contents of the leaves, cotton kernels, the mixture of fiber and seedcoat were all changed and positively correlated with the fiber color. Conclusion The three genes of GhCHS , GhANR and GhLAR were predominantly expressed early in developing colored cotton fibers and identified to be a key genes of cotton fiber color formation. The expression levels of the three genes affected the anthocyanidin contents and fiber color depth. So the three genes played a crucial part in cotton fiber color formation and has important significant to improve natural colored cotton quality and create new colored cotton germplasm resources by genetic engineering.

scholarly journals Functional analysis of GhCHS, GhANR and GhLAR in colored fiber formation of Gossypium hirsutum L.

2019 ◽  
Author(s):  
Gao Jianfang ◽  
Shen Li ◽  
Yuan Jingli ◽  
Zheng Hongli ◽  
Su Quansheng ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Cotton Fiber ◽  
Fiber Formation ◽  
Gossypium Hirsutum L ◽  
Expression Levels ◽  
Color Formation ◽  
Colored Cotton ◽  
Natural Colored Cotton ◽  
Key Genes ◽  
Color Depth

Abstract Background The formation of natural colored fibers mainly results from the accumulation of different anthocyanidins and their derivatives in the fibers of Gossypium hirsutum L. Chalcone synthase (CHS) is the first committed enzyme of flavonoid biosynthesis, and anthocyanidins are transported into fiber cell after biosynthesis mainly by Anthocyanidin reductase (ANR) and Leucoanthocyanidin reductase (LAR) to present diverse colors with distinct stability. The biochemical and molecular mechanism of pigment formation in natural colored cotton fiber is not clear. Results The three key genes of GhCHS , GhANR and GhLAR were predominantly expressed in the developing fibers of colored cotton. In the GhCHSi , GhANRi and GhLARi transgenic cottons, the expression levels of GhCHS , GhANR and GhLAR significantly decreased in the developing cotton fiber, negatively correlated with the content of anthocyanidins and the color depth of cotton fiber. In colored cotton Zongxu1 (ZX1) and the GhCHSi , GhANRi and GhLARi transgenic lines of ZX1, HZ and ZH, the anthocyanidin contents of the leaves, cotton kernels, the mixture of fiber and seedcoat were all changed and positively correlated with the fiber color. Conclusion The three genes of GhCHS , GhANR and GhLAR were predominantly expressed early in developing colored cotton fibers and identified to be a key genes of cotton fiber color formation. The expression levels of the three genes affected the anthocyanidin contents and fiber color depth. So the three genes played a crucial part in cotton fiber color formation and has important significant to improve natural colored cotton quality and create new colored cotton germplasm resources by genetic engineering.

scholarly journals Functional analysis of GhCHS, GhANR and GhLAR in colored fiber formation of Gossypium hirsutum L.

2019 ◽  
Author(s):  
Gao Jianfang ◽  
Shen Li ◽  
Yuan Jingli ◽  
Zheng Hongli ◽  
Su Quansheng ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Cotton Fiber ◽  
Fiber Formation ◽  
Gossypium Hirsutum L ◽  
Expression Levels ◽  
Color Formation ◽  
Colored Cotton ◽  
Natural Colored Cotton ◽  
Key Genes ◽  
Color Depth

Abstract Background The formation of natural colored fibers mainly results from the accumulation of different anthocyanidins and their derivatives in the fibers of Gossypium hirsutum L. Chalcone synthase (CHS) is the first committed enzyme of flavonoid biosynthesis, and anthocyanidins are transported into fiber cell after biosynthesis mainly by Anthocyanidin reductase (ANR) and Leucoanthocyanidin reductase (LAR) to present diverse colors with distinct stability. The biochemical and molecular mechanism of pigment formation in natural colored cotton fiber is not clear. Results The three key genes of GhCHS , GhANR and GhLAR were predominantly expressed in the developing fibers of colored cotton. In the GhCHSi , GhANRi and GhLARi transgenic cottons, the expression levels of GhCHS , GhANR and GhLAR significantly decreased in the developing cotton fiber, negatively correlated with the content of anthocyanidins and the color depth of cotton fiber. In colored cotton Zongxu1 (ZX1) and the GhCHSi , GhANRi and GhLARi transgenic lines of ZX1, HZ and ZH, the anthocyanidin contents of the leaves, cotton kernels, the mixture of fiber and seedcoat were all changed and positively correlated with the fiber color. Conclusion The three genes of GhCHS , GhANR and GhLAR were predominantly expressed early in developing colored cotton fibers and identified to be a key genes of cotton fiber color formation. The expression levels of the three genes affected the anthocyanidin contents and fiber color depth. So the three genes played a crucial part in cotton fiber color formation and has important significant to improve natural colored cotton quality and create new colored cotton germplasm resources by genetic engineering.

scholarly journals Functional analysis of GhCHS, GhANR and GhLAR in colored fiber formation of Gossypium hirsutum L

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Jianfang Gao ◽  
Li Shen ◽  
Jingli Yuan ◽  
Hongli Zheng ◽  
Quansheng Su ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Cotton Fiber ◽  
Fiber Formation ◽  
Pigment Formation ◽  
Expression Levels ◽  
Color Formation ◽  
Colored Cotton ◽  
Natural Colored Cotton ◽  
Key Genes ◽  
Color Depth

Abstract Background The formation of natural colored fibers mainly results from the accumulation of different anthocyanidins and their derivatives in the fibers of Gossypium hirsutum L. Chalcone synthase (CHS) is the first committed enzyme of flavonoid biosynthesis, and anthocyanidins are transported into fiber cells after biosynthesis mainly by Anthocyanidin reductase (ANR) and Leucoanthocyanidin reductase (LAR) to present diverse colors with distinct stability. The biochemical and molecular mechanism of pigment formation in natural colored cotton fiber is not clear. Results The three key genes of GhCHS, GhANR and GhLAR were predominantly expressed in the developing fibers of colored cotton. In the GhCHSi, GhANRi and GhLARi transgenic cottons, the expression levels of GhCHS, GhANR and GhLAR significantly decreased in the developing cotton fiber, negatively correlated with the content of anthocyanidins and the color depth of cotton fiber. In colored cotton Zongxu1 (ZX1) and the GhCHSi, GhANRi and GhLARi transgenic lines of ZX1, HZ and ZH, the anthocyanidin contents of the leaves, cotton kernels, the mixture of fiber and seedcoat were all changed and positively correlated with the fiber color. Conclusion The three genes of GhCHS, GhANR and GhLAR were predominantly expressed early in developing colored cotton fibers and identified to be a key genes of cotton fiber color formation. The expression levels of the three genes affected the anthocyanidin contents and fiber color depth. So the three genes played a crucial part in cotton fiber color formation and has important significant to improve natural colored cotton quality and create new colored cotton germplasm resources by genetic engineering.

scholarly journals Functional analysis of GhCHS, GhANR and GhLAR in colored fiber formation of G. hirsutum L

2019 ◽  
Author(s):  
Yuqiang Sun ◽  
Gao Jianfang ◽  
Shen Li ◽  
Yuan Jingli ◽  
Zheng Hongli ◽  
...  
Keyword(s):  
Cotton Fiber ◽  
Fiber Formation ◽  
Pigment Formation ◽  
Expression Levels ◽  
Color Formation ◽  
Colored Cotton ◽  
Transgenic Lines ◽  
Natural Colored Cotton ◽  
Key Genes ◽  
Color Depth

Abstract Background The formation of natural colored fibers mainly results from the accumulation of different anthocyanidins and their derivatives in the fibers. Chalcone synthase (CHS) is the first committed enzyme of flavonoid biosynthesis, anthocyanidins are transported into fiber cell after biosynthesis mainly by Anthocyanidin reductase (ANR) and Leucoanthocyanidin reductase (LAR) to present diverse colors with distinct stability. The biochemical and molecular mechanism of pigment formation in natural colored cotton fiber is not clear. Results The three key genes of GhCHS, GhANR and GhLAR were predominantly expressed in the developing fibers of colored cotton. In the GhCHSi, GhANRi and GhLARi transgenic cottons, the expression levels of GhCHS, GhANR and GhLAR significantly decreased in the developing cotton fiber, negatively correlated with the content of anthocyanidins and the color depth of cotton fiber. In colored cotton Zongxu1 (ZX1) and the GhCHSi, GhANRi and GhLARi transgenic lines of ZX1, HZ and ZH, the anthocyanidin contents of the leaves, cotton kernels, the mixture of fiber and seed coat were all changed and positively correlated with the fiber color. Conclusion The three genes of GhCHS, GhANR and GhLAR were predominantly expressed early in developing colored cotton fibers and identified to be a key genes of cotton fiber color formation. The expression levels of the three genes affected the anthocyanin contents and fiber color depth. So the three genes played a crucial part in cotton fiber color formation and has important significant to improve natural colored cotton quality and create new colored cotton germplasm resources by genetic engineering.

scholarly journals Identification of the Group III WRKY Subfamily and the Functional Analysis of GhWRKY53 in Gossypium hirsutum L.

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1235
Author(s):  
Dongjie Yang ◽  
Yuanyuan Liu ◽  
Hailiang Cheng ◽  
Qiaolian Wang ◽  
Limin Lv ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Cotton Fiber ◽  
Fiber Quality ◽  
Fiber Development ◽  
Amino Acid Sequences ◽  
Fiber Formation ◽  
Biotic And Abiotic Stress ◽  
Motif Analysis ◽  
Gossypium Hirsutum L ◽  
Group Iii

WRKY transcription factors had multiple functions in plant secondary metabolism, leaf senescence, fruit ripening, adaptation to biotic and abiotic stress, and plant growth and development. However, knowledge of the group III WRKY subfamily in fiber development in upland cotton (Gossypium hirsutum L.) is largely absent. Previous studies have shown that there were 21 putative group III WRKY members in G. hirsutum L. These putative amino acid sequences from the III WRKY group were phylogenetically clustered into three clades. Multiple alignment, conservative motif analysis, and gene structure analysis showed that the members clustered together in the phylogenetic tree had similar motifs and gene structures. Expression pattern analysis revealed that variation in the expression levels of these genes in different tissues and fiber development stages. To better understand the functions of putative group III WRKY genes in G. hirsutum L., we selected the cotton fiber initiation-related gene GhWRKY53 for cloning and functional identification. The subcellular localization experiment of GhWRKY53 in Nicotiana tabacum leaves showed that it was located in the nucleus. The heterologous expression of GhWRKY53 in Arabidopsis thaliana could significantly increase the density of trichomes. Twelve proteins that interacted with GhWRKY53 were screened from the cotton fiber cDNA library by yeast two-hybrid experiment. This study findings lay a foundation for further research on the role of the GhWRKY53 during cotton fiber development and provide a new insight for further studying putative group III WRKY genes in G. hirsutum L. Our research results also provide vital information for the genetic mechanism of high-quality cotton fiber formation and essential genetic resources for cotton fiber quality improvement.

scholarly journals Correction to: Functional analysis of GhCHS, GhANR and GhLAR in colored fiber formation of Gossypium hirsutum L

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Jianfang Gao ◽  
Li Shen ◽  
Jingli Yuan ◽  
Hongli Zheng ◽  
Quansheng Su ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Gossypium Hirsutum ◽  
Fiber Formation ◽  
Gossypium Hirsutum L

scholarly journals Functional genomics of fuzzless-lintless mutant of Gossypium hirsutum L. cv. MCU5 reveal key genes and pathways involved in cotton fibre initiation and elongation

BMC Genomics ◽  
2012 ◽  
Vol 13 (1) ◽  
pp. 624 ◽  
Author(s):  
Kethireddy Venkata Padmalatha ◽  
Deepak P Patil ◽  
Krishan Kumar ◽  
Gurusamy Dhandapani ◽  
Mogilicherla Kanakachari ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Functional Genomics ◽  
Cotton Fibre ◽  
Gossypium Hirsutum L ◽  
Key Genes

scholarly journals Controle biológico conservativo: Plantas herbáceas e a diversidade e abundância de dermápteros em algodoeiro colorido

2019 ◽  
Vol 12 (3) ◽  
pp. 132-140
Author(s):  
João Marcos Amario de Sousa ◽  
Danilo Henrique da Matta
Keyword(s):  
Biological Control ◽  
Gossypium Hirsutum ◽  
Fagopyrum Esculentum ◽  
Herbaceous Plants ◽  
Faunal Analysis ◽  
Pitfall Traps ◽  
Gossypium Hirsutum L ◽  
Weed Plants ◽  
Colored Cotton ◽  
Fagopyrum Esculentum Moench

Resumo. No presente estudo avaliou-se o efeito de plantas herbáceas floríferas (PHF) e plantas espontâneas (PE) sobre Dermaptera em algodoeiro colorido. O experimento foi conduzido em área com algodoeiro, Gossypium hirsutum L., cultivar BRS verde (Malvaceae), contendo bordas com plantas herbáceas floríferas (PHF) e plantas espontâneas (PE), durante o período de outubro/2011 a maio/2013. A amostragem foi quinzenal utilizando-se armadilhas tipo alçapão (Pitfall). Os dados obtidos foram submetidos às análises de fauna e variância. O total de 3.527 indivíduos e quatro espécies de Dermaptera foram capturados no algodoeiro colorido e plantas herbáceas. As espécies de dermápteros predominante foi Labidura riparia (Pallas). A maior diversidade de espécies de dermápteros ocorreu em plantas espontâneas (H'= 0,661) e o maior número de indivíduos de em Fagopyrum esculentum Moench e plantas espontâneas.Conservative biological control: Herbaceous plants on diversity and abundance of dermaptera in colored cottonAbstract. The aim of this study was to evaluate the effect of flowering herbaceous plants (FHP) and weed plants (WP) on Dermaptera in colored cotton. The study was conducted in colored cotton, Gossypium hirsutum L., BRS Verde, with FHP and WP, from October/2011 to May/2013. The sampling was fortnightly with pitfall traps. The data were submitted to faunal analysis and analysis of variance (ANOVA). The total of 3,527 individuals and four species of Dermaptera were captured in colored cotton and herbaceous plants. Labidura riparia (Pallas) were Dermaptera predominant, respectively. The higher diversity of species for Dermaptera the greater number was in Fagopyrum esculentum Moench and weed plants (H'= 0,661).

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