An operon consisting of a P-type ATPase gene and a transcriptional regulator gene responsible for cadmium resistances in Bacillus vietamensis 151-6 and Bacillus marisflavi 151-25

Abstract Background: Cadmium (Cd) is a severely toxic heavy metal to most microorganisms. Many bacteria have developed Cd 2+ resistance. Results: In this study, we isolated two different Cd 2+ resistance Bacillus sp . strains, Bacillus vietamensis 151-6 and Bacillus marisflavi 151-25 , which could be grown in the presence of Cd 2+ at concentration up to 0.3 mM and 0.8 mM, respectively. According to the genomic sequencing, transcriptome analysis under cadmium stress, and other related experiments, a gene cluster in plasmid p25 was found to be a major contributor to Cd 2+ resistance in B. marisflavi 151-25. The cluster in p25 contained orf4802 and orf4803 which encodes an ATPase transporter and a transcriptional regulator protein, respectively. Although 151-6 has much lower Cd 2+ resistance than 151-25, they contained similar gene cluster, but in different locations. A gene cluster on the chromosome containing orf4111 , orf4112 and orf4113 , which encodes an ATPase transporter, a cadmium efflux system accessory protein and a cadmium resistance protein, respectively, was found to play a major role on the Cd 2+ resistance for B. vietamensis 151-6. Conclusions: This work described cadmium resistance mechanisms in newly isolated Bacillus vietamensis 151-6 and Bacillus marisflavi 151-25. Based on homologies to the cad system (CadA-CadC) in Staphylococcus aureus and analysis of transcriptome under Cd 2+ induction, we inferred that the mechanisms of cadmium resistance in B. marisflavi 151-25 was as same as the cad system in S. aureus . Although Bacillus vietamensis 151-6 also had the similar gene cluster to B. marisflavi 151-25 and S. aureus , its transcriptional regulatory mechanism of cadmium resistance was not same. This study explored the cadmium resistance mechanism for B. vietamensis 151-6 and B. marisflavi 151-25 and has expanded our understanding of the biological effects of cadmium.

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An operon consisting of a P-type ATPase gene and a transcriptional regulator gene responsible for cadmium resistances in Bacillus vietamensis 151-6 and Bacillus marisflavi 151-25

10.21203/rs.2.11501/v6 ◽

2020 ◽

Author(s):

Xiaoxia Yu ◽

Zundan Ding ◽

Yangyang Ji ◽

Jintong Zhao ◽

Xiaoqing Liu ◽

...

Keyword(s):

Gene Cluster ◽

Biological Effects ◽

Resistance Mechanism ◽

Transcriptional Regulator ◽

Cadmium Stress ◽

Cadmium Resistance ◽

Toxic Heavy Metal ◽

Cad System ◽

Atpase Gene ◽

Similar Gene

Abstract Background: Cadmium (Cd) is a severely toxic heavy metal to most microorganisms. Many bacteria have developed Cd 2+ resistance. Results: In this study, we isolated two different Cd 2+ resistance Bacillus sp . strains, Bacillus vietamensis 151-6 and Bacillus marisflavi 151-25 , which could be grown in the presence of Cd 2+ at concentration up to 0.3 mM and 0.8 mM, respectively. According to the genomic sequencing, transcriptome analysis under cadmium stress, and other related experiments, a gene cluster in plasmid p25 was found to be a major contributor to Cd 2+ resistance in B. marisflavi 151-25. The cluster in p25 contained orf4802 and orf4803 which encodes an ATPase transporter and a transcriptional regulator protein, respectively. Although 151-6 has much lower Cd 2+ resistance than 151-25, they contained similar gene cluster, but in different locations. A gene cluster on the chromosome containing orf4111 , orf4112 and orf4113 , which encodes an ATPase transporter, a cadmium efflux system accessory protein and a cadmium resistance protein, respectively, was found to play a major role on the Cd 2+ resistance for B. vietamensis 151-6. Conclusions: This work described cadmium resistance mechanisms in newly isolated Bacillus vietamensis 151-6 and Bacillus marisflavi 151-25. Based on homologies to the cad system (CadA-CadC) in Staphylococcus aureus and analysis of transcriptome under Cd 2+ induction, we inferred that the mechanisms of cadmium resistance in B. marisflavi 151-25 was as same as the cad system in S. aureus . Although Bacillus vietamensis 151-6 also had the similar gene cluster to B. marisflavi 151-25 and S. aureus , its transcriptional regulatory mechanism of cadmium resistance was not same. This study explored the cadmium resistance mechanism for B. vietamensis 151-6 and B. marisflavi 151-25 and has expanded our understanding of the biological effects of cadmium.

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An operon consisting of a P-type ATPase gene and a transcriptional regulator gene responsible for cadmium resistances in Bacillus vietamensis 151-6 and Bacillus marisflavi 151-25

10.21203/rs.2.11501/v3 ◽

2020 ◽

Author(s):

Xiaoxia Yu ◽

Zundan Ding ◽

Yangyang Ji ◽

Jintong Zhao ◽

Xiaoqing Liu ◽

...

Keyword(s):

Gene Cluster ◽

Biological Effects ◽

Resistance Mechanism ◽

Transcriptional Regulator ◽

Cadmium Stress ◽

Cadmium Resistance ◽

Toxic Heavy Metal ◽

Cad System ◽

Atpase Gene ◽

Similar Gene

Abstract Background: Cadmium (Cd) is a severely toxic heavy metal to most microorganisms. Many bacteria have developed Cd 2+ resistance. Results: In this study, we isolated two different Cd 2+ resistance Bacillus sp . strains, Bacillus vietamensis 151-6 and Bacillus marisflavi 151-25 , which could be grown in the presence of Cd 2+ at concentration up to 0.3 mM and 0.8 mM, respectively. According to the genomic sequencing, transcriptome analysis under cadmium stress, and other related experiments, a gene cluster in plasmid p25 was found to be a major contributor to Cd 2+ resistance in B. marisflavi 151-25. The cluster in p25 contained orf4802 and orf4803 which encodes an ATPase transporter and a transcriptional regulator protein, respectively. Although 151-6 has much lower Cd 2+ resistance than 151-25, they contained similar gene cluster, but in different locations. A gene cluster on the chromosome containing orf4111 , orf4112 and orf4113 , which encodes an ATPase transporter, a cadmium efflux system accessory protein and a cadmium resistance protein, respectively, was found to play a major role on the Cd 2+ resistance for B. vietamensis 151-6. Conclusions: This work described cadmium resistance mechanisms in newly isolated Bacillus vietamensis 151-6 and Bacillus marisflavi 151-25. Based on homologies to the cad system (CadA-CadC) in Staphylococcus aureus and analysis of transcriptome under Cd 2+ induction, we inferred that the mechanisms of cadmium resistance in B. marisflavi 151-25 was as same as the cad system in S. aureus . Although Bacillus vietamensis 151-6 also had the similar gene cluster to B. marisflavi 151-25 and S. aureus , its transcriptional regulatory mechanism of cadmium resistance was not same. This study explored the cadmium resistance mechanism for B. vietamensis 151-6 and B. marisflavi 151-25 and has expanded our understanding of the biological effects of cadmium.

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An operon consisting of a P-type ATPase gene and a transcriptional regulator gene responsible for cadmium resistances in Bacillus vietamensis 151-6 and Bacillus marisflavi 151-25

10.21203/rs.2.11501/v2 ◽

2019 ◽

Author(s):

Xiaoxia Yu ◽

Zundan Ding ◽

Yangyang Ji ◽

Jintong Zhao ◽

Xiaoqing Liu ◽

...

Keyword(s):

Gene Cluster ◽

Biological Effects ◽

Resistance Mechanism ◽

Transcriptional Regulator ◽

Cadmium Stress ◽

Cadmium Resistance ◽

Toxic Heavy Metal ◽

Cad System ◽

Atpase Gene ◽

Similar Gene

Abstract Background: Cadmium (Cd) is a severely toxic heavy metal to most microorganisms. Many bacteria have developed Cd 2+ resistance. Results: In this study, we isolated two different Cd 2+ resistance Bacillus sp . strains, Bacillus vietamensis 151-6 and Bacillus marisflavi 151-25 , which could be grown in the presence of Cd 2+ at concentration up to 0.3 mM and 0.8 mM, respectively. According to the genomic sequencing, transcriptome analysis under cadmium stress, and other related experiments, a gene cluster in plasmid p25 was found to be a major contributor to Cd 2+ resistance in B. marisflavi 151-25. The cluster in p25 contained orf4802 and orf4803 which encodes an ATPase transporter and a transcriptional regulator protein, respectively. Although 151-6 has much lower Cd 2+ resistance than 151-25, they contained similar gene cluster, but in different locations. A gene cluster on the chromosome containing orf4111 , orf4112 and orf4113 , which encodes an ATPase transporter, a cadmium efflux system accessory protein and a cadmium resistance protein, respectively, was found to play a major role on the Cd 2+ resistance for B. vietamensis 151-6. Conclusions: This work described cadmium resistance mechanisms in newly isolated Bacillus vietamensis 151-6 and Bacillus marisflavi 151-25. Based on homologies to the cad system (CadA-CadC) in Staphylococcus aureus and analysis of transcriptome under Cd 2+ induction, we inferred that the mechanisms of cadmium resistance in B. marisflavi 151-25 was as same as the cad system in S. aureus . Although Bacillus vietamensis 151-6 also had the similar gene cluster to B. marisflavi 151-25 and S. aureus , its transcriptional regulatory mechanism of cadmium resistance was not same. This study explored the cadmium resistance mechanism for B. vietamensis 151-6 and B. marisflavi 151-25 and has expanded our understanding of the biological effects of cadmium.

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An operon consisting of a P-type ATPase gene and a transcriptional regular gene given the different cadmium resistances in Bacillus vietamensis 151-6 and Bacillus marisflavi 151-25

10.21203/rs.2.11501/v1 ◽

2019 ◽

Author(s):

Xiaoxia Yu ◽

Zundan Ding ◽

Yangyang Ji ◽

Jintong Zhao ◽

Xiaoqing Liu ◽

...

Keyword(s):

Gene Cluster ◽

Cadmium Stress ◽

Bacillus Sp ◽

Cadmium Resistance ◽

Toxic Heavy Metal ◽

Cad System ◽

Regulator Protein ◽

Atpase Gene ◽

Resistance Protein ◽

P Type

Abstract Cadmium (Cd) is a severely toxic heavy metal to most microorganisms. Many bacteria have developed Cd2+ resistance. In this study, we isolated two different Cd2+ resistance Bacillus sp. strains, Bacillus vietamensis 151-6 and Bacillus marisflavi 151-25, which could be grown in the presence of Cd2+ at concentration up to 0.3 mM and 0.8 mM, respectively. According to the genomic sequencing, transcriptome under cadmium stress and related biological experiments, a gene cluster in plasmid p25 containing orf4802 and orf4803, which encode an ATPase transporter and a transcriptional regulator protein, respectively, was a major contributor to Cd2+ resistance in B. marisflavi 151-25. Although 151-6 has much lower Cd2+ resistance than 151-25, they contain similar gene cluster, but in different locations. A gene cluster on the chromosome containing orf4111, orf4112 and orf4113, which encode an ATPase transporter, a cadmium efflux system accessory protein and a cadmium resistance protein, respectively, was found to be a major role on the Cd2+ resistance for B. vietamensis 151-6. Based on homologies to the cad system (CadA-CadC) in Staphylococcus aureus, the mechanisms of cadmium resistance in B. vietamensis 151-6 and B. marisflavi 151-25 were as same as the cad system. Our study on the cadmium mechanism for B. vietamensis 151-6 and B. marisflavi 151-25 proved preliminarily that the cad system is widespread in Bacillus sp. bacteria.

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The Connection between Czc and Cad Systems Involved in Cadmium Resistance in Pseudomonas putida

International Journal of Molecular Sciences ◽

10.3390/ijms22189697 ◽

2021 ◽

Vol 22 (18) ◽

pp. 9697

Author(s):

Huizhong Liu ◽

Yu Zhang ◽

Yingsi Wang ◽

Xiaobao Xie ◽

Qingshan Shi

Keyword(s):

Pseudomonas Putida ◽

Efflux Pump ◽

Response Regulator ◽

Cadmium Stress ◽

Cadmium Resistance ◽

Cad Systems ◽

Cad System ◽

Considerable Resistance ◽

High Level ◽

The Relationship

Heavy metal pollution is widespread and persistent, and causes serious harm to the environment. Pseudomonas putida, a representative environmental microorganism, has strong resistance to heavy metals due to its multiple efflux systems. Although the functions of many efflux systems have been well-studied, the relationship between them remains unclear. Here, the relationship between the Czc and Cad systems that are predominantly responsible for cadmium efflux in P. putida KT2440 is identified. The results demonstrated that CzcR3, the response regulator of two-component system CzcRS3 in the Czc system, activates the expression of efflux pump genes czcCBA1 and czcCBA2 by directly binding to their promoters, thereby helping the strain resist cadmium stress. CzcR3 can also bind to its own promoter, but it has only a weak regulatory effect. The high-level expression of czcRS3 needs to be induced by Cd2+, and this relies on the regulation of CadR, a key regulator in the Cad system, which showed affinity to czcRS3 promoter. Our study indicates that the Cad system is involved in the regulation of the Czc system, and this relationship is important for maintaining the considerable resistance to cadmium in P. putida.

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Identification of Putative Biosynthetic Gene Clusters for Tolyporphins in Multiple Filamentous Cyanobacteria

Life ◽

10.3390/life11080758 ◽

2021 ◽

Vol 11 (8) ◽

pp. 758

Author(s):

Xiaohe Jin ◽

Yunlong Zhang ◽

Ran Zhang ◽

Kathy-Uyen Nguyen ◽

Jonathan S. Lindsey ◽

...

Keyword(s):

Gene Cluster ◽

Gene Clusters ◽

Biosynthetic Gene Cluster ◽

Filamentous Cyanobacterium ◽

Biosynthetic Gene ◽

Filamentous Cyanobacteria ◽

Biosynthetic Gene Clusters ◽

Common Component ◽

Homology Searching ◽

Similar Gene

Tolyporphins A–R are unusual tetrapyrrole macrocycles produced by the non-axenic filamentous cyanobacterium HT-58-2. A putative biosynthetic gene cluster for biosynthesis of tolyporphins (here termed BGC-1) was previously identified in the genome of HT-58-2. Here, homology searching of BGC-1 in HT-58-2 led to identification of similar BGCs in seven other filamentous cyanobacteria, including strains Nostoc sp. 106C, Nostoc sp. RF31YmG, Nostoc sp. FACHB-892, Brasilonema octagenarum UFV-OR1, Brasilonema octagenarum UFV-E1, Brasilonema sennae CENA114 and Oculatella sp. LEGE 06141, suggesting their potential for tolyporphins production. A similar gene cluster (BGC-2) also was identified unexpectedly in HT-58-2. Tolyporphins BGCs were not identified in unicellular cyanobacteria. Phylogenetic analysis based on 16S rRNA and a common component of the BGCs, TolD, points to a close evolutionary history between each strain and their respective tolyporphins BGC. Though identified with putative tolyporphins BGCs, examination of pigments extracted from three cyanobacteria has not revealed the presence of tolyporphins. Overall, the identification of BGCs and potential producers of tolyporphins presents a collection of candidate cyanobacteria for genetic and biochemical analysis pertaining to these unusual tetrapyrrole macrocycles.

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A Novel CO-Responsive Transcriptional Regulator and Enhanced H2Production by an Engineered Thermococcus onnurineus NA1 Strain

Applied and Environmental Microbiology ◽

10.1128/aem.03019-14 ◽

2014 ◽

Vol 81 (5) ◽

pp. 1708-1714 ◽

Cited By ~ 25

Author(s):

Min-Sik Kim ◽

Ae Ran Choi ◽

Seong Hyuk Lee ◽

Hae-Chang Jung ◽

Seung Seob Bae ◽

...

Keyword(s):

Production Rate ◽

Gene Cluster ◽

Type Strain ◽

Wild Type Strain ◽

Regulatory System ◽

Transcriptional Regulator ◽

Bioreactor Culture ◽

Wild Type ◽

Content Type ◽

Transcriptional Regulatory

ABSTRACTGenome analysis revealed the existence of a putative transcriptional regulatory system governing CO metabolism inThermococcus onnurineusNA1, a carboxydotrophic hydrogenogenic archaeon. The regulatory system is composed of CorQ with a 4-vinyl reductase domain and CorR with a DNA-binding domain of the LysR-type transcriptional regulator family in close proximity to the CO dehydrogenase (CODH) gene cluster. Homologous genes of the CorQR pair were also found in the genomes ofThermococcusspecies and “CandidatusKorarchaeum cryptofilum” OPF8. In-frame deletion of eithercorQorcorRcaused a severe impairment in CO-dependent growth and H2production. WhencorQandcorRdeletion mutants were complemented by introducing thecorQRgenes under the control of a strong promoter, the mRNA and protein levels of the CODH gene were significantly increased in a ΔCorR strain complemented with integratedcorQR(ΔCorR/corQR↑) compared with those in the wild-type strain. In addition, the ΔCorR/corQR↑strain exhibited a much higher H2production rate (5.8-fold) than the wild-type strain in a bioreactor culture. The H2production rate (191.9 mmol liter−1h−1) and the specific H2production rate (249.6 mmol g−1h−1) of this strain were extremely high compared with those of CO-dependent H2-producing prokaryotes reported so far. These results suggest that thecorQRgenes encode a positive regulatory protein pair for the expression of a CODH gene cluster. The study also illustrates that manipulation of the transcriptional regulatory system can improve biological H2production.

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Transcriptional Regulation of the Nitrile Hydratase Gene Cluster in Pseudomonas chlororaphis B23

Journal of Bacteriology ◽

10.1128/jb.00061-08 ◽

2008 ◽

Vol 190 (12) ◽

pp. 4210-4217 ◽

Cited By ~ 19

Author(s):

Toshihide Sakashita ◽

Yoshiteru Hashimoto ◽

Ken-Ichi Oinuma ◽

Michihiko Kobayashi

Keyword(s):

Gene Cluster ◽

Nitrile Hydratase ◽

Transcriptional Regulator ◽

Pseudomonas Chlororaphis ◽

Reading Frame ◽

Transcription Start Sites ◽

Reverse Transcription Pcr ◽

Enormous Amount ◽

Extension Analysis ◽

Arac Family

ABSTRACT An enormous amount of nitrile hydratase (NHase) is inducibly produced by Pseudomonas chlororaphis B23 after addition of methacrylamide as the sole nitrogen source to a medium. The expression pattern of the P. chlororaphis B23 NHase gene cluster in response to addition of methacrylamide to the medium was investigated. Recently, we reported that the NHase gene cluster comprises seven genes (oxdA, amiA, nhpA, nhpB, nhpC, nhpS, and acsA). Sequence analysis of the 1.5-kb region upstream of the oxdA gene revealed the presence of a 936-bp open reading frame (designated nhpR), which should encode a protein with a molecular mass of 35,098. The deduced amino acid sequence of the nhpR product showed similarity to the sequences of transcriptional regulators belonging to the XylS/AraC family. Although the transcription of the eight genes (nhpR, oxdA, amiA, nhpABC, nhpS, and acsA) in the NHase gene cluster was induced significantly in the P. chlororaphis B23 wild-type strain after addition of methacrylamide to the medium, transcription of these genes in the nhpR disruptant was not induced, demonstrating that nhpR codes for a positive transcriptional regulator in the NHase gene cluster. A reverse transcription-PCR experiment revealed that five genes (oxdA, amiA, nhpA, nhpB, and nhpC) are cotranscribed, as are two other genes (nhpS and acsA). The transcription start sites for nhpR, oxdA, nhpA, and nhpS were mapped by primer extension analysis, and putative −12 and −24 σ54-type promoter binding sites were identified. NhpR was found to be the first transcriptional regulator of NHase belonging to the XylS/AraC family.

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Physiological and Biochemical Responses ofUlva proliferaandUlva linzato Cadmium Stress

The Scientific World JOURNAL ◽

10.1155/2013/289537 ◽

2013 ◽

Vol 2013 ◽

pp. 1-11 ◽

Cited By ~ 9

Author(s):

He-ping Jiang ◽

Bing-bing Gao ◽

Wen-hui Li ◽

Ming Zhu ◽

Chun-fang Zheng ◽

...

Keyword(s):

Amino Acids ◽

Photochemical Efficiency ◽

Cadmium Stress ◽

Nutrient Content ◽

Ulva Prolifera ◽

Cadmium Resistance ◽

Relative Growth ◽

Biochemical Responses ◽

Photochemical Efficiency Of Psii ◽

Physiological And Biochemical

Responses ofUlva proliferaandUlva linzato Cd2+stress were studied. We found that the relative growth rate (RGR), Fv/Fm, and actual photochemical efficiency of PSII (Yield) of twoUlvaspecies were decreased under Cd2+treatments, and these reductions were greater inU. proliferathan inU. linza.U. proliferaaccumulated more cadmium thanU. linzaunder Cd2+stress. WhileU. linzashowed positive osmotic adjustment ability (OAA) at a wider Cd2+range thanU. prolifera.U. linzahad greater contents of N, P, Na+, K+, and amino acids thanU. prolifera. A range of parameters (concentrations of cadmium, Ca2+, N, P, K+, Cl−, free amino acids (FAAs), proline, organic acids and soluble protein, Fv/Fm, Yield, OAA, and K+/Na+) could be used to evaluate cadmium resistance inUlvaby correlation analysis. In accordance with the order of the absolute values of correlation coefficient, contents of Cd2+and K+, Yield, proline content, Fv/Fm, FAA content, and OAA value ofUlvawere more highly related to their adaptation to Cd2+than the other eight indices. Thus,U. linzahas a better adaptation to Cd2+thanU. prolifera, which was due mainly to higher nutrient content and stronger OAA and photosynthesis inU. linza.

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