scholarly journals Effect of arsenic stress on 5-methylcytosine, photosynthetic parameters and nutrient content in arsenic hyperaccumulator Pteris cretica (L.) var. Albo-lineata

2020 ◽  
Author(s):  
Veronika Zemanová ◽  
Marek Popov ◽  
Daniela Pavlíková ◽  
Pavel Kotrba ◽  
František Hnilička ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Water Potential ◽  
Methylation Status ◽  
Nutrient Content ◽  
Physiological Parameters ◽  
Photosynthetic Parameters ◽  
High Exposure ◽  
Arsenic Accumulation ◽  
Pteris Cretica ◽  
As Toxicity

Abstract Background Arsenic toxicity induces a range of metabolic responses in plants, including DNA methylation. The focus of this paper was on the relationship between As-induced stress and plant senescence in the hyperaccumulator Pteris cretica var. Albo-lineata (Pc-Al). We assume difference in physiological parameters and level of DNA methylation in young and old fronds as symptoms of As toxicity. Results The As accumulation of Pc-Al fronds, grown in pots of haplic chernozem contaminated with 100 mg As kg-1 for 122 days, decreased with age. Content of As was higher in young than old fronds. The highest As content was determined in old fronds of Pc-Al grown in pots with 250 mg As kg-1. The increase with age was confirmed for determined nutrients – Cu, Mg, Mn, S and Zn. A significant elevation of analysed nutrients was showed in old fronds. Arsenic accumulation affected DNA methylation status in fronds, but content of 5-methylcytosine (5mC) decreased only in old fronds of Pc-Al (from 25% to 12%). Determined photosynthetic processes showed a decrease of fluorescence, photosynthetic rate and chlorophylls of As treatments in young and old fronds. Water potential was decreased by As in both fronds. Thinning of the sclerenchymatous inner cortex and a reduction in average tracheid metaxylem in the vascular cylinder was showed in roots of As treatment. Irrespective to fronds age, physiological parameters positively correlated with a 5mC while negatively with direct As toxicity. Opposite results were found for contents of Cu, Mg, Mn, S and Zn. Conclusions The results of this paper point to changes in the metabolism of the hyperaccumulator plant Pc-Al, upon low and high exposure to As contamination. The significant impact of As on DNA methylation was found in old fronds. Irrespective to fronds age, significant correlations were confirmed for 5mC and As toxicity. Our analysis of the very low water potential values and lignification of cell walls in roots showed that transports of assimilated metabolites and water between roots and fronds were reduced. As was showed by our results, epigenetic changes could affect studied parameters of the As hyperaccumulator Pc-Al, especially in old fronds.

scholarly journals Effect of arsenic stress on 5-methylcytosine, photosynthetic parameters and nutrient content in arsenic hyperaccumulator Pteris cretica (L.) var. Albo-lineata

2020 ◽  
Author(s):  
Veronika Zemanová ◽  
Marek Popov ◽  
Daniela Pavlíková ◽  
Pavel Kotrba ◽  
František Hnilička ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Water Potential ◽  
Methylation Status ◽  
Nutrient Content ◽  
Physiological Parameters ◽  
Photosynthetic Parameters ◽  
High Exposure ◽  
Arsenic Accumulation ◽  
Pteris Cretica ◽  
As Toxicity

Abstract Background Arsenic toxicity induces a range of metabolic responses in plants, including DNA methylation. The focus of this paper was on the relationship between As-induced stress and plant senescence in the hyperaccumulator Pteris cretica var. Albo-lineata (Pc -Al). We assume difference in physiological parameters and level of DNA methylation in young and old fronds as symptoms of As toxicity. Results The As accumulation of Pc -Al fronds, grown in pots of haplic chernozem contaminated with 100 mg As kg -1 for 122 days, decreased with age. Content of As was higher in young than old fronds. The highest As content was determined in old fronds of Pc -Al grown in pots with 250 mg As kg -1. The increase with age was confirmed for determined nutrients – Cu, Mg, Mn, S and Zn. A significant elevation of analysed nutrients was showed in old fronds. Arsenic accumulation affected DNA methylation status in fronds, but content of 5-methylcytosine (5mC) decreased only in old fronds of Pc -Al (from 25% to 12%). Determined photosynthetic processes showed a decrease of fluorescence, photosynthetic rate and chlorophylls of As treatments in both fronds. Water potential was decreased by As in both fronds. Thinning of the sclerenchymatous inner cortex and a reduction in average tracheid metaxylem in the vascular cylinder was showed in roots of As treatment. Irrespective to fronds age, physiological parameters positively correlated with a 5mC while negatively with direct As toxicity. Opposite results were found for contents of Cu, Mg, Mn, S and Zn. Conclusions The results of this paper point to changes in the metabolism of Pc -Al, upon low and high exposure to As contamination. The significant impact of As on DNA methylation was found in old fronds. Irrespective to fronds age, significant correlations were confirmed for 5mC and As toxicity. Our analysis of the very low water potential values and lignification of cell walls in roots showed that transports of assimilated metabolites and water between roots and fronds were reduced. As was showed by our results, epigenetic changes could affect studied parameters of As hyperaccumulator Pc -Al, especially in old fronds.

scholarly journals Effect of arsenic stress on 5-methylcytosine, photosynthetic parameters and nutrient content in arsenic hyperaccumulator Pteris cretica (L.) var. Albo-lineata

2020 ◽  
Author(s):  
Veronika Zemanová ◽  
Marek Popov ◽  
Daniela Pavlíková ◽  
Pavel Kotrba ◽  
František Hnilička ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Water Potential ◽  
Methylation Status ◽  
Nutrient Content ◽  
Physiological Parameters ◽  
Photosynthetic Parameters ◽  
High Exposure ◽  
Pteris Cretica ◽  
As Toxicity ◽  
Hyperaccumulator Plant

Abstract Background: Arsenic toxicity induces a range of metabolic responses in plants, including DNA methylation. The focus of this paper was on the relationship between As-induced stress and plant senescence in the hyperaccumulator Pteris cretica var. Albo-lineata (Pc-Al). We assume difference in physiological parameters and level of DNA methylation in young and old fronds as symptoms of As toxicity.Results: The As accumulation of Pc-Al fronds, grown in pots of haplic chernozem contaminated with 100 mg As kg-1 for 122 days, decreased with age. Content of As was higher in young than old fronds for variants with 100 mg As kg-1 (2800 and 2000 mg As kg-1 dry matter, respectively). The highest As content was determined in old fronds of Pc-Al grown in pots with 250 mg As kg-1. The increase with age was confirmed for determined nutrients – Cu, Mg, Mn, S and Zn. A significant elevation of all analysed nutrients was showed in old fronds. Arsenic accumulation affected DNA methylation status in fronds, but content of 5-methylcytosine (5mC) decreased only in old fronds of Pc-Al (from 25% to 12%). Determined photosynthetic processes showed a decrease of fluorescence, photosynthetic rate and chlorophylls of As treatments in young and old fronds. Water potential was decreased by As in both fronds. Thinning of the sclerenchymatous inner cortex and a reduction in average tracheid metaxylem in the vascular cylinder was showed in roots of As treatment. Irrespective to fronds age, physiological parameters positively correlated with a 5mC while negatively with direct As toxicity. Opposite results were found for contents of Cu, Mg, Mn, S and Zn.Conclusions: The results of this paper point to changes in the metabolism of the hyperaccumulator plant Pc-Al, upon low and high exposure to As contamination. The significant impact of As on DNA methylation was found in old fronds. Irrespective to fronds age, significant correlations were confirmed for 5mC and As toxicity. Our analysis of the very low water potential values and lignification of cell walls in roots showed that transports of assimilated metabolites and water between roots and fronds were reduced. As was showed by our results, epigenetic changes could affect studied parameters of the As hyperaccumulator plant Pc-Al, especially in old fronds.

scholarly journals Effect of arsenic stress on 5-methylcytosine, photosynthetic parameters and nutrient content in arsenic hyperaccumulator Pteris cretica (L.) var. Albo-lineata

2019 ◽  
Author(s):  
Veronika Zemanová ◽  
Marek Popov ◽  
Daniela Pavlíková ◽  
Pavel Kotrba ◽  
František Hnilička ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Water Potential ◽  
Methylation Status ◽  
Nutrient Content ◽  
Physiological Parameters ◽  
Photosynthetic Parameters ◽  
Arsenic Accumulation ◽  
Pteris Cretica ◽  
As Toxicity ◽  
Hyperaccumulator Plant

Abstract Background Arsenic toxicity induces a range of metabolic responses in plants, including DNA methylation. The focus of this paper was on the relationship between As-induced stress and plant senescence in the hyperaccumulator Pteris cretica var. Albo-lineata (Pc-Al). We assume difference in physiological parameters and level of DNA methylation in young and old fronds as first symptoms of As low toxicity. Results The As accumulation of Pc-Al fronds, grown in pots of haplic chernozem contaminated with 100 mg As kg-1 for 122 days, decreased with age. Content of As was higher in young than old fronds (2800 and 2000 mg As kg-1 dry matter, respectively). Opposite trend – increase with age, was confirmed for determined nutrients – Cu, Mg, Mn, S and Zn. A significant elevation of all analysed nutrients was showed in old fronds. Arsenic accumulation affected DNA methylation status in fronds, but content of 5-methylcytosine (5mC) decreased only in old fronds of Pc-Al (from 25% to 15%). Determined photosynthetic processes showed a decrease of fluorescence, photosynthetic rate and chlorophylls of As treatment in young and old fronds. Water potential was decreased by As in both fronds. Thinning of the sclerenchymatous inner cortex and a reduction in average tracheid metaxylem in the vascular cylinder was showed in roots of As treatment. Irrespective to fronds age, physiological parameters correlated more significantly with a 5mC than with direct As toxicity. Similar trend was found for contents of Cu, Mg, Mn, S and Zn. Conclusions The results of this paper point to first changes in the metabolism of the hyperaccumulator plant Pc-Al, upon exposure to As contamination. The significant impact of As on DNA methylation was found in old fronds. Irrespective to fronds age, the most significant correlations were confirmed for 5mC, in contrast to direct As toxicity. Our analysis of the very low water potential values and lignification of cell walls in roots showed that transports of assimilated metabolites and water between roots and fronds were reduced. As was showed by our results, epigenetic changes could affect studied parameters of the As hyperaccumulator plant Pc-Al, especially in old fronds.

scholarly journals Effect of arsenic stress on 5-methylcytosine, photosynthetic parameters and nutrient content in Pteris cretica var. Albo-lineata hyperaccumulator

2019 ◽  
Author(s):  
Veronika Zemanová ◽  
Marek Popov ◽  
Daniela Pavlíková ◽  
Pavel Kotrba ◽  
František Hnilička ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Methylation Status ◽  
Nutrient Content ◽  
Control Treatment ◽  
Photosynthetic Parameters ◽  
Gas Exchange Parameters ◽  
Pteris Cretica ◽  
Arsenic Stress ◽  
As Toxicity

Abstract Background Arsenic (As) toxicity induces a range of metabolic responses in plants, including DNA methylation. The focus of this paper was on the relationship between As-induced long-term stress and plant senescence in the hyperaccumulator Pteris cretica var. Albo-lineata ( Pc -Al).Results We showed that Pc -Al grown in pots of haplic chernozem contaminated with 100 mg As kg -1 (As 100 ) for 122 days could accumulate more than 2000 and 2800 mg As kg -1 dry matter in old and young fronds, respectively. Analysis of 5-methylcytosine (5mC) in Pc -Al confirmed that the overall DNA methylation status in fronds of As 100 ferns was reduced in contrast to control treatment. Compared with controls, the overall DNA methylation status in fronds of As 100 ferns was reduced (by 6% in young and 10% in old fronds); however, the decrease was significant only in old fronds. The significant correlations for 5mC, in contrast to direct As toxicity, showed that decreases of chlorophylls, fluorescence and photosynthetic rate could be affected by epigenetic changes. Photosynthetic processes were determined in As 100 treatment and showed a reduction of gas-exchange parameters, and a decrease in carotenoids and chlorophylls (by 5% and 26%, respectively). Hyperaccumulation of As resulted in a significant elevation of all analysed nutrients (Cu, Mn, Zn, Mg, S) in old fronds, but not in young fronds.Conclusions The results of this paper point to complex changes in the metabolism of the hyperaccumulator plant Pc -Al, upon exposure to As contamination. The most significant impact was found in young fronds. The physiological parameters correlated more significantly with a decrease of DNA methylation than with direct As toxicity. Our analysis of the very low water potential values and lignification of cell walls in roots showed that transports of assimilated metabolites and water between roots and fronds were reduced.

scholarly journals Transcriptome and Methylome Changes in Two Contrasting Mungbean Genotypes in Response to Drought Stress

2021 ◽  
Author(s):  
Peilei Zhao ◽  
Bao Ma ◽  
Chunmei Cai ◽  
Jihua Xu
Keyword(s):  
Dna Methylation ◽  
Genetic Variation ◽  
Drought Stress ◽  
Stress Responses ◽  
Methylation Status ◽  
Physiological Parameters ◽  
Gene Body ◽  
Sequence Context ◽  
Transcriptomic Data ◽  
Growth Distribution

Abstract Background: Due to drought stress, the growth, distribution, and production of mungbean is severely restricted. Previous study combining physiological and transcriptomic data indicated different genotypes of mungbean exhibited variable responses when exposed to drought stress. Aside from the genetic variation, the modifications of environmentally induced epigenetics alterations on mungbean drought-stress responses were still elusive. Results: In this study, firstly, we compared the drought tolerance capacity at seedling stages by detecting physiological parameters in two contrasting genotypes wild mungbean 61 and cultivar 70 in response to drought stress. We found that wild mungbean 61 showed lower level of MDA and higher levels of SOD, POD, and CAT, suggesting wild mungbean 61 exhibited stronger drought resistances. Transcriptomic analysis indicated totally 2659 differentially expressed genes (DEGs) were detected when 61 compared with 70 (C61 vs C70), and the number increased to 3121 in the comparison of drought-treated 70 compared with drought-treated 61 (D70 vs D61). In addition, when drought-treated 61 and 70 were compared with their controls, the DEGs were 1117 and 185 respectively, with more down-regulated DEGs than up-regulated in D61 vs C61, which was opposite in D70 vs C70. Interestingly, corresponding to this, after drought stress, more hypermethylated differentially methylated regions (DMRs) in 61 were detected and more hypomethylated DMRs in 70 were detected. Further analysis suggested that the main variations between 61 and 70 existed in CHH methylation in promoter. Moreover, the preference of methylation status alterations in D60 vs C60 and D70 vs C70 also fell in CHH sequence context. Further analysis of the correlation between DMRs and DEGs indicated in both D61 vs C61 and D70 vs C70, the DMRs in gene body was significantly negatively correlated with DEGs. Conclusion: The physiological parameters in this research suggested that wild mungbean 61 was more resistant to drought stress, with more hypermethylated DMRs and less hypomethylated DMRs after drought stress, corresponding to more down-regulated DEGs than up-regulated DEGs. Among the three DNA methylation contexts CG, CHG, and CHH, asymmetric CHH contexts were more dynamic and prone to be altered by drought stress and genotypic variations.

DNA Methylation Status of Mash2 in Lungs of Somatic Cell Cloning Bovines*

2010 ◽  
Vol 37 (9) ◽  
pp. 960-966 ◽  
Author(s):  
Jie CHEN ◽  
Dong-Jie LI ◽  
Cui ZHANG ◽  
Ning LI ◽  
Shi-Jie LI
Keyword(s):  
Dna Methylation ◽  
Somatic Cell ◽  
Methylation Status ◽  
Cell Cloning

scholarly journals Epigenetic Changes During Mechanically Induced Osteogenic Lineage Commitment

2015 ◽  
Vol 137 (2) ◽  
Author(s):  
Julia C. Chen ◽  
Mardonn Chua ◽  
Raymond B. Bellon ◽  
Christopher R. Jacobs
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Shear Stress ◽  
Fluid Shear Stress ◽  
Methylation Status ◽  
Lineage Commitment ◽  
Fluid Shear ◽  
Osteogenic Lineage ◽  
Osteogenic Markers ◽  
Heterochromatin Structure

Osteogenic lineage commitment is often evaluated by analyzing gene expression. However, many genes are transiently expressed during differentiation. The availability of genes for expression is influenced by epigenetic state, which affects the heterochromatin structure. DNA methylation, a form of epigenetic regulation, is stable and heritable. Therefore, analyzing methylation status may be less temporally dependent and more informative for evaluating lineage commitment. Here we analyzed the effect of mechanical stimulation on osteogenic differentiation by applying fluid shear stress for 24 hr to osteocytes and then applying the osteocyte-conditioned medium (CM) to progenitor cells. We analyzed gene expression and changes in DNA methylation after 24 hr of exposure to the CM using quantitative real-time polymerase chain reaction and bisulfite sequencing. With fluid shear stress stimulation, methylation decreased for both adipogenic and osteogenic markers, which typically increases availability of genes for expression. After only 24 hr of exposure to CM, we also observed increases in expression of later osteogenic markers that are typically observed to increase after seven days or more with biochemical induction. However, we observed a decrease or no change in early osteogenic markers and decreases in adipogenic gene expression. Treatment of a demethylating agent produced an increase in all genes. The results indicate that fluid shear stress stimulation rapidly promotes the availability of genes for expression, but also specifically increases gene expression of later osteogenic markers.

scholarly journals DNA methylation differs extensively between strains of the same geographical origin and changes with age in Daphnia magna

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Jack Hearn ◽  
Fiona Plenderleith ◽  
Tom J. Little
Keyword(s):  
Dna Methylation ◽  
Caloric Restriction ◽  
Daphnia Magna ◽  
Single Gene ◽  
Methylation Status ◽  
Strain Differences ◽  
Life History Trait ◽  
Food Level ◽  
Epigenetic Clock ◽  
Age Related

Abstract Background Patterns of methylation influence lifespan, but methylation and lifespan may also depend on diet, or differ between genotypes. Prior to this study, interactions between diet and genotype have not been explored together to determine their influence on methylation. The invertebrate Daphnia magna is an excellent choice for testing the epigenetic response to the environment: parthenogenetic offspring are identical to their siblings (making for powerful genetic comparisons), they are relatively short lived and have well-characterised inter-strain life-history trait differences. We performed a survival analysis in response to caloric restriction and then undertook a 47-replicate experiment testing the DNA methylation response to ageing and caloric restriction of two strains of D. magna. Results Methylated cytosines (CpGs) were most prevalent in exons two to five of gene bodies. One strain exhibited a significantly increased lifespan in response to caloric restriction, but there was no effect of food-level CpG methylation status. Inter-strain differences dominated the methylation experiment with over 15,000 differently methylated CpGs. One gene, Me31b, was hypermethylated extensively in one strain and is a key regulator of embryonic expression. Sixty-one CpGs were differentially methylated between young and old individuals, including multiple CpGs within the histone H3 gene, which were hypermethylated in old individuals. Across all age-related CpGs, we identified a set that are highly correlated with chronological age. Conclusions Methylated cytosines are concentrated in early exons of gene sequences indicative of a directed, non-random, process despite the low overall DNA methylation percentage in this species. We identify no effect of caloric restriction on DNA methylation, contrary to our previous results, and established impacts of caloric restriction on phenotype and gene expression. We propose our approach here is more robust in invertebrates given genome-wide CpG distributions. For both strain and ageing, a single gene emerges as differentially methylated that for each factor could have widespread phenotypic effects. Our data showed the potential for an epigenetic clock at a subset of age positions, which is exciting but requires confirmation.

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