scholarly journals Effect of arsenic stress on 5-methylcytosine, photosynthetic parameters and nutrient content in Pteris cretica var. Albo-lineata hyperaccumulator

2019 ◽  
Author(s):  
Veronika Zemanová ◽  
Marek Popov ◽  
Daniela Pavlíková ◽  
Pavel Kotrba ◽  
František Hnilička ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Methylation Status ◽  
Nutrient Content ◽  
Control Treatment ◽  
Photosynthetic Parameters ◽  
Gas Exchange Parameters ◽  
Pteris Cretica ◽  
Arsenic Stress ◽  
As Toxicity

Abstract Background Arsenic (As) toxicity induces a range of metabolic responses in plants, including DNA methylation. The focus of this paper was on the relationship between As-induced long-term stress and plant senescence in the hyperaccumulator Pteris cretica var. Albo-lineata ( Pc -Al).Results We showed that Pc -Al grown in pots of haplic chernozem contaminated with 100 mg As kg -1 (As 100 ) for 122 days could accumulate more than 2000 and 2800 mg As kg -1 dry matter in old and young fronds, respectively. Analysis of 5-methylcytosine (5mC) in Pc -Al confirmed that the overall DNA methylation status in fronds of As 100 ferns was reduced in contrast to control treatment. Compared with controls, the overall DNA methylation status in fronds of As 100 ferns was reduced (by 6% in young and 10% in old fronds); however, the decrease was significant only in old fronds. The significant correlations for 5mC, in contrast to direct As toxicity, showed that decreases of chlorophylls, fluorescence and photosynthetic rate could be affected by epigenetic changes. Photosynthetic processes were determined in As 100 treatment and showed a reduction of gas-exchange parameters, and a decrease in carotenoids and chlorophylls (by 5% and 26%, respectively). Hyperaccumulation of As resulted in a significant elevation of all analysed nutrients (Cu, Mn, Zn, Mg, S) in old fronds, but not in young fronds.Conclusions The results of this paper point to complex changes in the metabolism of the hyperaccumulator plant Pc -Al, upon exposure to As contamination. The most significant impact was found in young fronds. The physiological parameters correlated more significantly with a decrease of DNA methylation than with direct As toxicity. Our analysis of the very low water potential values and lignification of cell walls in roots showed that transports of assimilated metabolites and water between roots and fronds were reduced.

scholarly journals Effect of arsenic stress on 5-methylcytosine, photosynthetic parameters and nutrient content in arsenic hyperaccumulator Pteris cretica (L.) var. Albo-lineata

2020 ◽  
Author(s):  
Veronika Zemanová ◽  
Marek Popov ◽  
Daniela Pavlíková ◽  
Pavel Kotrba ◽  
František Hnilička ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Water Potential ◽  
Methylation Status ◽  
Nutrient Content ◽  
Physiological Parameters ◽  
Photosynthetic Parameters ◽  
High Exposure ◽  
Pteris Cretica ◽  
As Toxicity ◽  
Hyperaccumulator Plant

Abstract Background: Arsenic toxicity induces a range of metabolic responses in plants, including DNA methylation. The focus of this paper was on the relationship between As-induced stress and plant senescence in the hyperaccumulator Pteris cretica var. Albo-lineata (Pc-Al). We assume difference in physiological parameters and level of DNA methylation in young and old fronds as symptoms of As toxicity.Results: The As accumulation of Pc-Al fronds, grown in pots of haplic chernozem contaminated with 100 mg As kg-1 for 122 days, decreased with age. Content of As was higher in young than old fronds for variants with 100 mg As kg-1 (2800 and 2000 mg As kg-1 dry matter, respectively). The highest As content was determined in old fronds of Pc-Al grown in pots with 250 mg As kg-1. The increase with age was confirmed for determined nutrients – Cu, Mg, Mn, S and Zn. A significant elevation of all analysed nutrients was showed in old fronds. Arsenic accumulation affected DNA methylation status in fronds, but content of 5-methylcytosine (5mC) decreased only in old fronds of Pc-Al (from 25% to 12%). Determined photosynthetic processes showed a decrease of fluorescence, photosynthetic rate and chlorophylls of As treatments in young and old fronds. Water potential was decreased by As in both fronds. Thinning of the sclerenchymatous inner cortex and a reduction in average tracheid metaxylem in the vascular cylinder was showed in roots of As treatment. Irrespective to fronds age, physiological parameters positively correlated with a 5mC while negatively with direct As toxicity. Opposite results were found for contents of Cu, Mg, Mn, S and Zn.Conclusions: The results of this paper point to changes in the metabolism of the hyperaccumulator plant Pc-Al, upon low and high exposure to As contamination. The significant impact of As on DNA methylation was found in old fronds. Irrespective to fronds age, significant correlations were confirmed for 5mC and As toxicity. Our analysis of the very low water potential values and lignification of cell walls in roots showed that transports of assimilated metabolites and water between roots and fronds were reduced. As was showed by our results, epigenetic changes could affect studied parameters of the As hyperaccumulator plant Pc-Al, especially in old fronds.

scholarly journals Effect of arsenic stress on 5-methylcytosine, photosynthetic parameters and nutrient content in arsenic hyperaccumulator Pteris cretica (L.) var. Albo-lineata

2020 ◽  
Author(s):  
Veronika Zemanová ◽  
Marek Popov ◽  
Daniela Pavlíková ◽  
Pavel Kotrba ◽  
František Hnilička ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Water Potential ◽  
Methylation Status ◽  
Nutrient Content ◽  
Physiological Parameters ◽  
Photosynthetic Parameters ◽  
High Exposure ◽  
Arsenic Accumulation ◽  
Pteris Cretica ◽  
As Toxicity

Abstract Background Arsenic toxicity induces a range of metabolic responses in plants, including DNA methylation. The focus of this paper was on the relationship between As-induced stress and plant senescence in the hyperaccumulator Pteris cretica var. Albo-lineata (Pc-Al). We assume difference in physiological parameters and level of DNA methylation in young and old fronds as symptoms of As toxicity. Results The As accumulation of Pc-Al fronds, grown in pots of haplic chernozem contaminated with 100 mg As kg-1 for 122 days, decreased with age. Content of As was higher in young than old fronds. The highest As content was determined in old fronds of Pc-Al grown in pots with 250 mg As kg-1. The increase with age was confirmed for determined nutrients – Cu, Mg, Mn, S and Zn. A significant elevation of analysed nutrients was showed in old fronds. Arsenic accumulation affected DNA methylation status in fronds, but content of 5-methylcytosine (5mC) decreased only in old fronds of Pc-Al (from 25% to 12%). Determined photosynthetic processes showed a decrease of fluorescence, photosynthetic rate and chlorophylls of As treatments in young and old fronds. Water potential was decreased by As in both fronds. Thinning of the sclerenchymatous inner cortex and a reduction in average tracheid metaxylem in the vascular cylinder was showed in roots of As treatment. Irrespective to fronds age, physiological parameters positively correlated with a 5mC while negatively with direct As toxicity. Opposite results were found for contents of Cu, Mg, Mn, S and Zn. Conclusions The results of this paper point to changes in the metabolism of the hyperaccumulator plant Pc-Al, upon low and high exposure to As contamination. The significant impact of As on DNA methylation was found in old fronds. Irrespective to fronds age, significant correlations were confirmed for 5mC and As toxicity. Our analysis of the very low water potential values and lignification of cell walls in roots showed that transports of assimilated metabolites and water between roots and fronds were reduced. As was showed by our results, epigenetic changes could affect studied parameters of the As hyperaccumulator Pc-Al, especially in old fronds.

scholarly journals Effect of arsenic stress on 5-methylcytosine, photosynthetic parameters and nutrient content in arsenic hyperaccumulator Pteris cretica (L.) var. Albo-lineata

2019 ◽  
Author(s):  
Veronika Zemanová ◽  
Marek Popov ◽  
Daniela Pavlíková ◽  
Pavel Kotrba ◽  
František Hnilička ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Water Potential ◽  
Methylation Status ◽  
Nutrient Content ◽  
Physiological Parameters ◽  
Photosynthetic Parameters ◽  
Arsenic Accumulation ◽  
Pteris Cretica ◽  
As Toxicity ◽  
Hyperaccumulator Plant

Abstract Background Arsenic toxicity induces a range of metabolic responses in plants, including DNA methylation. The focus of this paper was on the relationship between As-induced stress and plant senescence in the hyperaccumulator Pteris cretica var. Albo-lineata (Pc-Al). We assume difference in physiological parameters and level of DNA methylation in young and old fronds as first symptoms of As low toxicity. Results The As accumulation of Pc-Al fronds, grown in pots of haplic chernozem contaminated with 100 mg As kg-1 for 122 days, decreased with age. Content of As was higher in young than old fronds (2800 and 2000 mg As kg-1 dry matter, respectively). Opposite trend – increase with age, was confirmed for determined nutrients – Cu, Mg, Mn, S and Zn. A significant elevation of all analysed nutrients was showed in old fronds. Arsenic accumulation affected DNA methylation status in fronds, but content of 5-methylcytosine (5mC) decreased only in old fronds of Pc-Al (from 25% to 15%). Determined photosynthetic processes showed a decrease of fluorescence, photosynthetic rate and chlorophylls of As treatment in young and old fronds. Water potential was decreased by As in both fronds. Thinning of the sclerenchymatous inner cortex and a reduction in average tracheid metaxylem in the vascular cylinder was showed in roots of As treatment. Irrespective to fronds age, physiological parameters correlated more significantly with a 5mC than with direct As toxicity. Similar trend was found for contents of Cu, Mg, Mn, S and Zn. Conclusions The results of this paper point to first changes in the metabolism of the hyperaccumulator plant Pc-Al, upon exposure to As contamination. The significant impact of As on DNA methylation was found in old fronds. Irrespective to fronds age, the most significant correlations were confirmed for 5mC, in contrast to direct As toxicity. Our analysis of the very low water potential values and lignification of cell walls in roots showed that transports of assimilated metabolites and water between roots and fronds were reduced. As was showed by our results, epigenetic changes could affect studied parameters of the As hyperaccumulator plant Pc-Al, especially in old fronds.

scholarly journals Effect of arsenic stress on 5-methylcytosine, photosynthetic parameters and nutrient content in arsenic hyperaccumulator Pteris cretica (L.) var. Albo-lineata

2020 ◽  
Author(s):  
Veronika Zemanová ◽  
Marek Popov ◽  
Daniela Pavlíková ◽  
Pavel Kotrba ◽  
František Hnilička ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Water Potential ◽  
Methylation Status ◽  
Nutrient Content ◽  
Physiological Parameters ◽  
Photosynthetic Parameters ◽  
High Exposure ◽  
Arsenic Accumulation ◽  
Pteris Cretica ◽  
As Toxicity

Abstract Background Arsenic toxicity induces a range of metabolic responses in plants, including DNA methylation. The focus of this paper was on the relationship between As-induced stress and plant senescence in the hyperaccumulator Pteris cretica var. Albo-lineata (Pc -Al). We assume difference in physiological parameters and level of DNA methylation in young and old fronds as symptoms of As toxicity. Results The As accumulation of Pc -Al fronds, grown in pots of haplic chernozem contaminated with 100 mg As kg -1 for 122 days, decreased with age. Content of As was higher in young than old fronds. The highest As content was determined in old fronds of Pc -Al grown in pots with 250 mg As kg -1. The increase with age was confirmed for determined nutrients – Cu, Mg, Mn, S and Zn. A significant elevation of analysed nutrients was showed in old fronds. Arsenic accumulation affected DNA methylation status in fronds, but content of 5-methylcytosine (5mC) decreased only in old fronds of Pc -Al (from 25% to 12%). Determined photosynthetic processes showed a decrease of fluorescence, photosynthetic rate and chlorophylls of As treatments in both fronds. Water potential was decreased by As in both fronds. Thinning of the sclerenchymatous inner cortex and a reduction in average tracheid metaxylem in the vascular cylinder was showed in roots of As treatment. Irrespective to fronds age, physiological parameters positively correlated with a 5mC while negatively with direct As toxicity. Opposite results were found for contents of Cu, Mg, Mn, S and Zn. Conclusions The results of this paper point to changes in the metabolism of Pc -Al, upon low and high exposure to As contamination. The significant impact of As on DNA methylation was found in old fronds. Irrespective to fronds age, significant correlations were confirmed for 5mC and As toxicity. Our analysis of the very low water potential values and lignification of cell walls in roots showed that transports of assimilated metabolites and water between roots and fronds were reduced. As was showed by our results, epigenetic changes could affect studied parameters of As hyperaccumulator Pc -Al, especially in old fronds.

scholarly journals Differential DNA methylation by early versus late parenteral nutrition in the PICU: a biological basis for its impact on emotional and behavioral problems documented 4 years later

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
An Jacobs ◽  
Fabian Güiza ◽  
Ines Verlinden ◽  
Karolijn Dulfer ◽  
Gonzalo Garcia Guerra ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Parenteral Nutrition ◽  
Critically Ill ◽  
Behavioral Problems ◽  
Methylation Status ◽  
Critically Ill Children ◽  
Biological Basis ◽  
Cpg Sites ◽  
The Impact

Abstract Background The PEPaNIC multicenter randomized controlled trial (RCT) has shown that early administration of supplemental parenteral nutrition (early-PN) as compared with withholding PN for 1 week (late-PN) induced long-term internalizing, externalizing and total emotional/behavioral problems in critically ill children, as observed 4 years later. Early-PN was further shown to alter the methylation status of 37 CpG-sites in leukocyte DNA between admission and discharge from the pediatric intensive care unit (PICU). In a preplanned subanalysis of the PEPaNIC trial, we now investigated whether the altered methylation of these CpG-sites could statistically explain the negative impact of early-PN on emotion/behavior documented 4 years after PICU admission. Results The combination of DNA methylation data and data on behavior 4 years after PICU admission was available for 403 of the 1440 patients (aged 0–17 years at PICU admission) who were included in the PEPaNIC RCT (192 early-PN and 211 late-PN patients). Mediation analyses with use of bootstrapped multivariable non-linear regression analyses adjusted for baseline risk factors revealed that the adverse alterations by early-PN in methylation of the 37 CpG-sites together statistically explained its harmful impact on internalizing, externalizing and total emotional/behavioral problems. When adding the methylation status of the 37 CpG-sites to the models, the explanatory power improved with a 1.710 to 1.851-fold increase, and the impact of the altered methylation status of the CpG-sites explained the impact of the randomization to early-PN versus late-PN. Conclusions Abnormal DNA methylation induced by the early use of PN in the PICU provides a biological basis for its long-term harmful effect on emotion/behavior of critically ill children 4 years after PICU admission. Trial Registration ClinicalTrials.gov NCT01536275, registered February 17, 2012, https://clinicaltrials.gov/ct2/show/NCT01536275.

DNMT1 modulation in chronic hepatitis B patients and hypothetic influence on mitochondrial DNA methylation status during long-term nucleo(t)side analogs therapy

2017 ◽  
Vol 89 (7) ◽  
pp. 1208-1214 ◽  
Author(s):  
Giordano Madeddu ◽  
Silvia Ortu ◽  
Giovanni Garrucciu ◽  
Ivana Maida ◽  
Michela Melis ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Mitochondrial Dna ◽  
Chronic Hepatitis ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
Methylation Status

scholarly journals Long-Term Disturbed Expression and DNA Methylation of SCAP/SREBP Signaling in the Mouse Lung From Assisted Reproductive Technologies

2021 ◽  
Vol 12 ◽  
Author(s):  
Fang Le ◽  
Ning Wang ◽  
Qijing Wang ◽  
Xinyun Yang ◽  
Lejun Li ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Target Genes ◽  
Assisted Reproductive Technologies ◽  
Methylation Status ◽  
Reproductive Technologies ◽  
Mouse Lung ◽  
Aberrant Methylation ◽  
Aberrant Expression ◽  
Expression Levels

Assisted reproductive technology (ART) has been linked to cholesterol metabolic and respiratory disorders later in life, but the mechanisms by which biosynthetic signaling remain unclear. Lung inflammatory diseases are tightly linked with the sterol regulatory element-binding protein (SREBP) and SREBP cleavage-activating protein (SCAP), but this has not been shown in an ART offspring. Here, mouse models from a young to old age were established including in vitro fertilization (IVF), intracytoplasmic injection (ICSI), and in vivo fertilized groups. In our results, significantly higher plasma levels of CRP, IgM, and IgG were identified in the aged ICSI mice. Additionally, pulmonary inflammation was found in four aged ART mice. At three weeks, ART mice showed significantly downregulated levels of Scap, Srebp-1a, Srebp-1c, and Srebf2 mRNA in the lung. At the same time, significant differences in the DNA methylation rates of Scap-Srebfs and protein expression of nuclear forms of SREBPs (nSREBPs) were detected in the ART groups. Only abnormalities in the expression levels of Srebp-1a and Srebp-1c mRNA and nSREBP1 protein were found in the ART groups at 10 weeks. However, at 1.5 years old, aberrant expression levels and DNA methylation of SCAP, SREBP1, and SREBP2, and their associated target genes, were observed in the lung of the ART groups. Our results indicate that ART increases long-term alterations in SCAP/SREBP expression that may be associated with their aberrant methylation status in mouse.

scholarly journals 089 EFFECT OF ROOTSTOCK ON GROWTH, PRODUCTIVITY, AND FRUIT COMPOSITION OF ZINFANDEL GRAPEVINES

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 440f-441
Author(s):  
R.K. Striegler ◽  
D.R. Wineman
Keyword(s):  
Experimental Design ◽  
Yield Components ◽  
Nutrient Content ◽  
San Joaquin Valley ◽  
Control Treatment ◽  
Long Term Effects ◽  
Fruit Composition ◽  
Four Seasons ◽  
Shoot Number

Selected rootstocks were evaluated for four seasons in a Zinfandel vineyard located in the northern San Joaquin Valley of California. The vineyard was drip-irrigated and vineyard spacing was 2.1m × 3.3m (vine × row). A two-wire vertical trellis system was used and row orientation was east to west. Vines were trained to a bilateral cordon and spur-pruned. Rootstocks included in the study were: AxR #1, Freedom, Harmony, Kober 5BB, and Teleki 5C. Vines grafted on AxR #1 rootstock were considered to be the control treatment. A randomized complete block experimental design was used. There were six blocks and plots consisted of five vines. Data collected included yield, components of yield, fruit composition, bloom petiole nutrient content, shoot number, and pruning weight. After the initial season, yields were consistently highest for vines grafted on Freedom rootstock, with yields only slightly lower on AxR #1 rootstock. Significantly lower yields were recorded for vines grafted on Kober 5BB and Teleki 5C. Rootstock did not have a consistent effect on fruit composition. Shoots/vine and shoots/meter of canopy were not significantly effected by rootstock. Dormant pruning weight was highest for Freedom and lowest for Kober 5BB. These results suggest that rootstock selection can influence vineyard productivity in the northern San Joaquin Valley. Continued research is needed to determine long term effects of the rootstocks used in this study.

scholarly journals Free amino acid regulation in fronds and roots of two Pteris cretica L. ferns under arsenic stress

2020 ◽  
Vol 66 (No. 10) ◽  
pp. 483-492
Author(s):  
Veronika Zemanová ◽  
Daniela Pavlíková ◽  
Milan Pavlík
Keyword(s):  
Amino Acid ◽  
Free Amino Acid ◽  
Carbon Metabolism ◽  
Free Amino ◽  
Characteristic Change ◽  
Pteris Cretica ◽  
Arsenic Stress ◽  
As Stress ◽  
The Relationship ◽  
As Toxicity

In the present study, free amino acid (AA) regulation in the arsenic (As) hyperaccumulating ferns was evaluated in a pot experiment to determine the relationship between As stress and the characteristic change in metabolism of AAs. The ferns Pteris cretica cv. Albo-lineata (Pc-Al) and cv. Parkerii (Pc-Pa) were exposed to As treatments at 0, 20, 100, and 250 mg As/kg for 90 days. Greater As content, as well as higher biomass production, were identified in Pc-Al compared with Pc-Pa. Ferns showed changes in the stress metabolism of free AA homeostasis. These results indicate a disturbance in nitrogen metabolism and depletion of pool assimilated carbon metabolism. In the fronds and roots, Pc-Pa accumulated higher amounts of free AAs than Pc-Al. The total free AA content, as well as the ratio of the main AA family pathway (glutamate family), were increased by the accumulation of toxic As in the ferns. Results suggest that Pc-Al tolerates higher As doses better due to changes in AA biosynthesis; however, at higher As doses, Pc-Pa upregulated AA biosynthesis due to As toxicity. The most abundant free AAs of ferns was glutamine, which was enhanced by As. Furthermore, the ratios of selected individual free AAs revealed a characteristic phenotype difference between ferns.  

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