Nitric oxide is involved in the brassinolide-induced adventitious root development in cucumber

Abstract Background: Brassinolide (BR), as a new type of plant hormones, is involved in the processes of plant growth and stress response. Previous studies have reported the roles of BR in regulating plant developmental processes and also response tolerance to abiotic stresses in plants. The main purpose of our study was to explore whether nitric oxide (NO) plays a role in the process of BR-induced adventitious root formation in cucumber ( Cucumis sativus L.). Results: Exogenous application of 1 μM BR significantly promoted adventitious rooting, while high concentrations of BR (2-8 μM) effectively inhibited adventitious rooting. NO donor (S-nitroso-N-acerylpenicillamine, SNAP) promoted the occurrence of adventitious roots. Simultaneously, BR and SNAP applied together significantly promoted adventitious rooting and the combined effect was superior to the application of BR or SNAP alone. Moreover, NO scavenger (c-PTIO) and inhibitors (L-NAME and Tungstate) inhibited the positive effects of BR on adventitious rooting. BR at 1 μM also increased endogenous NO content, NO synthase (NOS-like) and Nitrate reductase (NR) activities, while BRz (a specific BR biosynthesis inhibitor) decreased these effects. In addition, the relative expression level of NR was up-regulated by BR and SNAP, whereas BRz down-regulated it. The application of NO inhibitor (Tungstate) in BR also inhibited the up-regulation of NR . Conclusion: BR promoted the formation of adventitious roots by inducing the production of endogenous NO in cucumber.

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Nitric oxide is involved in the brassinolide-induced adventitious root development in cucumber

10.21203/rs.2.15514/v2 ◽

2019 ◽

Author(s):

Li Yutong ◽

Yue Wu ◽

Weibiao Liao ◽

Linli Hu ◽

Mohammed Mujitaba Dawuda ◽

...

Keyword(s):

Nitric Oxide ◽

Adventitious Root ◽

Adventitious Roots ◽

Adventitious Rooting ◽

Adventitious Root Formation ◽

No Donor ◽

Cucumis Sativus L ◽

Positive Effects ◽

Relative Expression Level ◽

High Concentrations

Abstract Background: Brassinolide (BR), as a new type of plant hormones, is involved in the processes of plant growth and stress response. Previous studies have reported the roles of BR in regulating plant developmental processes and also response tolerance to abiotic stresses in plants. The main purpose of our study was to explore whether nitric oxide (NO) plays a role in the process of BR-induced adventitious root formation in cucumber (Cucumis sativus L.). Results: Exogenous application of 1 μM BR significantly promoted adventitious rooting, while high concentrations of BR (2-8 μM) effectively inhibited adventitious rooting. NO donor (S-nitroso-N-acerylpenicillamine, SNAP) promoted the occurrence of adventitious roots. Simultaneously, BR and SNAP applied together significantly promoted adventitious rooting and the combined effect was superior to the application of BR or SNAP alone. Moreover, NO scavenger (c-PTIO) and inhibitors (L-NAME and Tungstate) inhibited the positive effects of BR on adventitious rooting. BR at 1 μM also increased endogenous NO content, NO synthase (NOS-like) and Nitrate reductase (NR) activities, while BRz (a specific BR biosynthesis inhibitor) decreased these effects. In addition, the relative expression level of NR was up-regulated by BR and SNAP, whereas BRz down-regulated it. The application of NO inhibitor (Tungstate) in BR also inhibited the up-regulation of NR. Conclusion: BR promoted the formation of adventitious roots by inducing the production of endogenous NO in cucumber.

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Nitric oxide is involved in the brassinolide-induced adventitious root development in cucumber

10.21203/rs.2.15514/v5 ◽

2020 ◽

Author(s):

Li Yutong ◽

Yue Wu ◽

Weibiao Liao ◽

Linli Hu ◽

Mohammed Mujitaba Dawuda ◽

...

Keyword(s):

Nitric Oxide ◽

Adventitious Root ◽

Adventitious Roots ◽

Adventitious Rooting ◽

Adventitious Root Formation ◽

No Donor ◽

Positive Effects ◽

Relative Expression Level ◽

New Type ◽

High Concentrations

Abstract Background: Brassinolide (BR), as a new type of plant hormones, is involved in the processes of plant growth and stress response. Previous studies have reported the roles of BR in regulating plant developmental processes and also response tolerance to abiotic stresses in plants. The main purpose of our study was to explore whether nitric oxide (NO) plays a role in the process of BR-induced adventitious root formation in cucumber (Cucumis sativus L.). Results: Exogenous application of 1 μM BR significantly promoted adventitious rooting, while high concentrations of BR (2-8 μM) effectively inhibited adventitious rooting. NO donor (S-nitroso-N-acerylpenicillamine, SNAP) promoted the occurrence of adventitious roots. Simultaneously, BR and SNAP applied together significantly promoted adventitious rooting and the combined effect was superior to the application of BR or SNAP alone. Moreover, NO scavenger (c-PTIO) and inhibitors (L-NAME and Tungstate) inhibited the positive effects of BR on adventitious rooting. BR at 1 μM also increased endogenous NO content, NO synthase (NOS-like) and Nitrate reductase (NR) activities, while BRz (a specific BR biosynthesis inhibitor) decreased these effects. In addition, the relative expression level of NR was up-regulated by BR and SNAP, whereas BRz down-regulated it. The application of NO inhibitor (Tungstate) in BR also inhibited the up-regulation of NR. Conclusion: BR promoted the formation of adventitious roots by inducing the production of endogenous NO in cucumber.

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Nitric oxide is involved in the brassinolide-induced adventitious root development in cucumber (Cucumis sativus L.) explants

10.21203/rs.2.15514/v1 ◽

2019 ◽

Author(s):

Li Yutong ◽

Yue Wu ◽

Weibiao Liao ◽

Linli Hu ◽

Mohammed Mujitaba Dawuda ◽

...

Keyword(s):

Nitric Oxide ◽

Cucumis Sativus ◽

Adventitious Root ◽

Adventitious Roots ◽

Adventitious Rooting ◽

Adventitious Root Formation ◽

No Donor ◽

Cucumis Sativus L ◽

Positive Effects ◽

Relative Expression Level

Abstract Background: Brassinolide (BR), as a new type of plant hormones, is involved in the process of plant growth and stress response. Previous studies have reported the roles of BR in regulating plant developmental processes and also response tolerance to abiotic stresses in plants. In my study, the main purpose is to explore whether nitric oxide (NO) is involved in the process of BR-induced adventitious root formation in cucumber (Cucumis sativus L.), and whether it plays a certain role. Results: Exogenous application of 1 μM BR significantly promoted adventitious rooting, while high concentrations of BR (2-8 μM) effectively inhibited adventitious rooting. NO donor (S-nitroso-N-acerylpenicillamine, SNAP) promoted the occurrence of adventitious roots. Simultaneously, BR and SNAP mixed treatment significantly promoted adventitious rooting and the promoted effects was significantly superior to the application of BR or SNAP alone. Moreover, NO scavenger (c-PTIO) and inhibitors (L-NAME and Tungstate) inhibited the positive effects of BR on adventitious rooting. BR at 1 μM also increased endogenous NO content, NO synthase (NOS-like) and Nitrate reductase (NR) activities, while BRz (a specific BR biosynthesis inhibitor) decreased these effects. In addition, the relative expression level of NR was up-regulated by BR and SNAP, whereas BRz down-regulated it. The application of NO inhibitor (Tungstate) in BR also inhibited the up-regulation of NR. Conclusion: BR promoted the formation of adventitious roots via inducing the generation of endogenous NO.

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Nitric oxide participates in waterlogging tolerance through enhanced adventitious root formation in the euhalophyte Suaeda salsa

Functional Plant Biology ◽

10.1071/fp15120 ◽

2016 ◽

Vol 43 (3) ◽

pp. 244 ◽

Cited By ~ 48

Author(s):

Tianshu Chen ◽

Fang Yuan ◽

Jie Song ◽

Baoshan Wang

Keyword(s):

Nitric Oxide ◽

Adventitious Root ◽

Adventitious Roots ◽

Root Formation ◽

Reductase Activity ◽

Adventitious Root Formation ◽

Suaeda Salsa ◽

Cell Integrity ◽

Waterlogging Tolerance ◽

No Donor

The interactions of NO and other signalling molecules contribute to adventitious root formation in many plant species. To our knowledge, the role of NO in the adventitious root formation of plants subjected to waterlogging are as yet unknown. Populations of Suaeda salsa L., a C3 euhalophytic plant, from inland saline sites develop several adventitious roots in response to waterlogging. The NO donor sodium nitroprusside (SNP) and the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethyl-imidazoline-1–1-oxyl-3-oxide (cPTIO) were applied to S. salsa seedlings to examine the effects of NO on flooding tolerance and its possible mechanism. SNP alleviated growth inhibition and increased adventitious root formation, endogenous NO levels and adventitious root cell integrity in S. salsa subjected to waterlogging. These SNP-mediated effects were prevented by the extra application of cPTIO. SNP treatment decreased nitrate reductase activity but increased nitric oxide synthase (NOS) activity in adventitious roots. These results suggest that in S. salsa, NO participates in waterlogging tolerance by enhancing adventitious root formation and that NO generation is associated with the NOS-associated pathway.

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Some Urea Derivatives Positively Affect Adventitious Root Formation: Old Concepts and the State of the Art

Plants ◽

10.3390/plants9030321 ◽

2020 ◽

Vol 9 (3) ◽

pp. 321 ◽

Cited By ~ 1

Author(s):

Ricci Ada ◽

Rolli Enrico

Keyword(s):

Adventitious Root ◽

Root Formation ◽

Developmental Process ◽

Adventitious Rooting ◽

Adventitious Root Formation ◽

Point Of View ◽

Urea Derivatives ◽

Positive Effects ◽

Differentiated Cells ◽

Structure Activity

The success of vegetative propagation programmes strongly depends on adventitious rooting, a postembryonic developmental process whereby new roots can be induced from differentiated cells in positions where normally they do not arise. This auxin-dependent organogenesis has been studied at molecular, cellular, and developmental levels, and our knowledge of the process has improved in recent years. However, bioactive compounds that enhance adventitious root formation and possibly reduce undesirable auxinic side effects are still needed to ameliorate this process. From this point of view, our structure–activity relationship studies concerning urea derivatives revealed that some of them, more specifically, the N,N′-bis-(2,3-methylenedioxyphenyl)urea (2,3-MDPU), the N,N′-bis-(3,4-methylenedioxyphenyl)urea (3,4-MDPU), the 1,3-di(benzo[d]oxazol-5-yl)urea (5-BDPU), and the 1,3-di(benzo[d]oxazol-6-yl)urea (6-BDPU), constitute a category of adventitious rooting adjuvants. The results of our studies are presented here, in order either to highlight the positive effects of the supplementation of these urea derivatives, or to better understand the nature of their interaction with auxin.

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What Makes Adventitious Roots?

Plants ◽

10.3390/plants8070240 ◽

2019 ◽

Vol 8 (7) ◽

pp. 240 ◽

Cited By ~ 6

Author(s):

Gonin ◽

Bergougnoux ◽

Nguyen ◽

Gantet ◽

Champion

Keyword(s):

Root System ◽

Adventitious Root ◽

Adventitious Roots ◽

Root Formation ◽

Primary Root ◽

Adventitious Rooting ◽

Adventitious Root Formation ◽

Terrestrial Environment ◽

Root Types ◽

Embryonic Root

The spermatophyte root system is composed of a primary root that develops from an embryonically formed root meristem, and of different post-embryonic root types: lateral and adventitious roots. Adventitious roots, arising from the stem of the plants, are the main component of the mature root system of many plants. Their development can also be induced in response to adverse environmental conditions or stresses. Here, in this review, we report on the morphological and functional diversity of adventitious roots and their origin. The hormonal and molecular regulation of the constitutive and inducible adventitious root initiation and development is discussed. Recent data confirmed the crucial role of the auxin/cytokinin balance in adventitious rooting. Nevertheless, other hormones must be considered. At the genetic level, adventitious root formation integrates the transduction of external signals, as well as a core auxin-regulated developmental pathway that is shared with lateral root formation. The knowledge acquired from adventitious root development opens new perspectives to improve micropropagation by cutting in recalcitrant species, root system architecture of crops such as cereals, and to understand how plants adapted during evolution to the terrestrial environment by producing different post-embryonic root types.

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Tissue culture of Arabidopsis thaliana explants reveals a stimulatory effect of alkamides on adventitious root formation and nitric oxide accumulation

Plant Science ◽

10.1016/j.plantsci.2007.11.003 ◽

2008 ◽

Vol 174 (2) ◽

pp. 165-173 ◽

Cited By ~ 29

Author(s):

Juan Carlos Campos-Cuevas ◽

Ramón Pelagio-Flores ◽

Javier Raya-González ◽

Alfonso Méndez-Bravo ◽

Randy Ortiz-Castro ◽

...

Keyword(s):

Nitric Oxide ◽

Tissue Culture ◽

Arabidopsis Thaliana ◽

Adventitious Root ◽

Root Formation ◽

Adventitious Root Formation

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The microRNA476a/RFL module regulates adventitious root formation through a mitochondria-dependent pathway in Populus

10.1101/852178 ◽

2019 ◽

Author(s):

Changzheng Xu ◽

Yuanxun Tao ◽

Xiaokang Fu ◽

Li Guo ◽

Haitao Xing ◽

...

Keyword(s):

Adventitious Root ◽

Developmental Plasticity ◽

Adventitious Rooting ◽

Adventitious Root Formation ◽

Auxin Signaling ◽

Stem Cuttings ◽

Pentatricopeptide Repeat ◽

Dynamic Regulation ◽

Mitochondrial Regulation ◽

Mitochondrial Homeostasis

AbstractAdventitious root (AR) formation at the base of stem cuttings determines the efficiency of clonal propagation for woody plants. Many endogenous and environmental factors influence AR formation. However, our knowledge about the regulation of AR development by mitochondrial metabolism in plants is very limited. Here we identified Populus-specific miR476a as a novel regulator of wound-induced adventitious rooting via orchestrating mitochondrial homeostasis in poplar. MiR476a exhibited inducible expression during AR formation and directly targets several Restorer of Fertility like (RFL) genes encoding mitochondrion-localized pentatricopeptide repeat proteins. Genetic modification of miR476-RFL expression revealed the miR476/RFL-mediated dynamic regulation of mitochondrial homeostasis on AR formation in transgenic poplar. Furthermore, mitochondrial perturbation via exogenous chemical inhibitor validated that the miR476a/RFL-directed AR formation depended on mitochondrial regulation though modulating the auxin pathway. Our results established a miRNA-directed mitochondrion-auxin signaling cascade required for AR development, providing novel insights into the understanding of mitochondrial regulation on plant developmental plasticity.

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