scholarly journals Exogenous Jaagsiekte Sheep Retrovirus type 2 (exJSRV2) related to ovine pulmonary adenocarcinoma (OPA) in Romania: prevalence, anatomical forms, pathological description, immunophenotyping and virus identification

2020 ◽  
Author(s):  
Corina Toma ◽  
Valentin Bâlteanu ◽  
Septiumiu Tripon ◽  
Adrian Trifa ◽  
Alexandra Rema ◽  
...  
Keyword(s):  
Pulmonary Adenocarcinoma ◽  
Neoplastic Disease ◽  
Ovine Pulmonary Adenocarcinoma ◽  
Jaagsiekte Sheep Retrovirus ◽  
Virus Identification ◽  
Atypical Form ◽  
Eastern European Countries ◽  
The Uk ◽  
First Time

Abstract Background. Ovine pulmonary adenocarcinoma (OPA) is a neoplastic disease caused by exogenous Jaagsiekte Sheep Retrovirus (exJSRV). The prevalence of JSRV-related OPA in Eastern European countries, including Romania is unknown. We aimed to investigate: the prevalence and morphological features of OPA (classical and atypical forms) in Romania, the immunophenotype of the pulmonary tumors and their relationships with exJSRV infection. Methods. Lung tumors from 34 slaughtered adult ewes were grossly evaluated. Cytology, histology, immunocytochemistry, immunohistochemistry, electron microscopy and DNA testing were subsequently performed. Results. Out of 2693 examined sheep, 34 had OPA (1.26% prevalence). The diaphragmatic lobes were the most affected. Grossly, the classical OPA was identified in 88.24% of investigated cases and the atypical OPA in 11.76% that included solitary myxomatous nodules. Histopathology results confirmed the presence of OPA in all suspected cases, which were classified into acinar and papillary types. Myxoid growths (MGs) were diagnosed in 6 classical OPA cases and in 2 cases of atypical form. Lung adenocarcinoma was positive for MCK and TTF-1, and MGs showed immunoreaction for Vimentin, Desmin and SMA; Ki67 expression of classical OPA was higher than atypical OPA and MGs. JSRV-MA was identified by IHC (94.11%) in both epithelial and mesenchymal cells of OPA. ICC and TEM also confirmed the JSRV within the neoplastic cells. ExJSRV was identified by PCR in 97.05% of analyzed samples. Phylogenetic analysis revealed the presence of the exJSRV type 2 in Romanian sheep affected by lung cancer and showed a high similarity with the UK strain (AF105220.1). Conclusions. In this study, we confirmed for the first time in Romania the presence of exJSRV in naturally occurring OPA in sheep. Additionally, we described the first report of atypical OPA in Romania, and to the best of our knowledge, in Eastern Europe. Finally, we showed that MGs have a myofibroblastic origin.

scholarly journals Exogenous Jaagsiekte Sheep Retrovirus type 2 related to ovine pulmonary adenocarcinoma in Romania: prevalence, anatomical forms, pathological description, immunophenotyping and virus identification

2019 ◽  
Author(s):  
Corina Toma ◽  
Valentin Bâlteanu ◽  
Septiumiu Tripon ◽  
Adrian Trifa ◽  
Alexandra Rema ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Pulmonary Adenocarcinoma ◽  
Neoplastic Disease ◽  
Ovine Pulmonary Adenocarcinoma ◽  
Jaagsiekte Sheep Retrovirus ◽  
Virus Identification ◽  
Atypical Form ◽  
Eastern European Countries ◽  
The Uk

Abstract Ovine pulmonary adenocarcinoma (OPA) is a neoplastic disease caused by exogenous Jaagsiekte Sheep Retrovirus (exJSRV). The prevalence of JSRV-related OPA in Eastern European countries, including Romania is unknown. We aimed to investigate: the prevalence and morphological features of OPA (classical and atypical forms) in Romania, the immunophenotype of the pulmonary tumors and their relationships with exJSRV infection. Lung tumors from 28 slaughtered adult sheep and 6 necropsied individuals were grossly evaluated. Cytology, histology, immunocytochemistry, immunohistochemistry, electron microscopy and DNA testing were subsequently performed. Out of 2693 examined sheep, 34 had OPA (1.26% prevalence). The diaphragmatic lobe was the most affected. Grossly, the classical OPA was identified in 88.24% of investigated cases and the atypical OPA in 11.76% that included solitary myxomatous nodules. Histopathology results confirmed the presence of OPA in all suspected cases, which were classified into acinar and papillary types. Myxoid growths (MGs) were diagnosed in 6 classical OPA cases and in 2 cases of atypical form. JSRV-MA was identified by IHC (94.11%) in both epithelial and mesenchymal cells of OPA. Immunocytochemistry and electron microscopy also confirmed the JSRV within the neoplastic cells. Adenocarcinoma was positive for MCK and TTF-1, and MGs showed immunoreaction for Vimentin, Desmin and SMA; Ki67 expression of classical OPA was higher than atypical OPA and MGs. Phylogenetic analysis revealed the presence of the exJSRV type 2 in Romanian sheep affected by lung cancer and showed a high similarity with the UK strain (AF105220.1), confirming exJSRV2 for the first time in Romania. Additionally, we described the first report of atypical OPA in Romania, and to the best of our knowledge, in Eastern Europe. Finally, we showed that MGs have a myofibroblastic origin.

scholarly journals Detection of Jaagsiekte sheep retrovirus in apparently healthy sheep by real-time TaqMan PCR in comparison with histopathological findings

2016 ◽  
Vol 60 (1) ◽  
pp. 7-12 ◽  
Author(s):  
Aliasghar Bahari ◽  
Masoud Sabouri Ghannad ◽  
Omid Dezfoulian ◽  
Fereydon Rezazadeh ◽  
Ali Sadeghi-Nasab
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Mediastinal Lymph Node ◽  
Pulmonary Adenocarcinoma ◽  
Ovine Pulmonary Adenocarcinoma ◽  
Jaagsiekte Sheep Retrovirus ◽  
Tissue Samples ◽  
Blood Samples ◽  
Proviral Dna ◽  
Healthy Sheep

Abstract Introduction: The aim of this study was to use TaqMan real-time PCR technique to investigate Jaagsiekte sheep retrovirus (JSRV) proviral DNA in whole blood samples of sheep, and compare the results to those of histopathological examinations. Material and Methods: Eighty blood samples from clinically healthy sheep were randomly collected before the animals were slaughtered. Ten tissue samples from each lung and associated caudal mediastinal lymph node were taken. Results: Fifteen (18.75%) blood samples were found to contain proviral DNA, and 11 (13.75%) corresponding lung samples showed microscopic changes consistent with ovine pulmonary adenocarcinoma. None of the samples displayed metastases to the caudal mediastinal lymph nodes. The prominent pattern of neoplastic nodules consisted of acinar (alveolar) form. Conclusion: The results indicated the higher sensitivity of real-time PCR compared to histopathological examinations in detection of ovine pulmonary adenocarcinoma.

scholarly journals Alveolar Type II Cells Expressing Jaagsiekte Sheep Retrovirus Capsid Protein and Surfactant Proteins Are the Predominant Neoplastic Cell Type in Ovine Pulmonary Adenocarcinoma

2002 ◽  
Vol 39 (3) ◽  
pp. 341-352 ◽  
Author(s):  
J. A. Platt ◽  
N. Kraipowich ◽  
F. A. Villafane ◽  
J. C. DeMartini
Keyword(s):  
Tumor Cells ◽  
Capsid Protein ◽  
Surfactant Protein ◽  
Neoplastic Cell ◽  
Pulmonary Adenocarcinoma ◽  
Clara Cell ◽  
Alveolar Type ◽  
Type Ii ◽  
Ovine Pulmonary Adenocarcinoma ◽  
Jaagsiekte Sheep Retrovirus

Ovine pulmonary adenocarcinoma is caused by jaagsiekte sheep retrovirus. To gain insight into the histogenesis and viral pathogenesis of this neoplasm, the tumor cell phenotypes and differentiation state were correlated with the distribution of jaagsiekte sheep retrovirus capsid protein in neoplastic and normal cells of the lung in nine naturally occurring and 12 experimentally induced cases of ovine pulmonary adenocarcinoma. Overall, 82% of tumor cells had ultrastructural features consistent with alveolar type II cells, 7% of tumor cells had features of Clara cells, and 11% of tumor cells were insufficiently differentiated to classify. The proportion of the neoplastic cell phenotypes varied within tumors, and no tumor consisted of a morphologically uniform cell population. To further characterize the neoplastic cell population, sections of tumors were immunostained with antibodies to surfactant protein A, surfactant protein C, and Clara cell 10-kd protein. Overall, surfactant proteins A and C were expressed in 70% and 80% of tumor cells, respectively, whereas Clara cell 10-kd protein was expressed in 17% of tumor cells. Jaagsiekte sheep retrovirus capsid protein was detected in 71% of tumor cells and in macrophages (5/21 tumors examined) and in nonneoplastic alveolar and bronchiolar cells (6/14 tumors). Expression of this viral protein in neoplastic cells, classified morphologically and by immunophenotyping primarily as of the alveolar type II lineage, implies an important role for specific virus–cell interactions in the pathogenesis of ovine pulmonary adenocarcinoma.

scholarly journals Systemic immune responses following infection with Jaagsiekte sheep retrovirus and in the terminal stages of ovine pulmonary adenocarcinoma

2002 ◽  
Vol 83 (7) ◽  
pp. 1753-1757 ◽  
Author(s):  
Christina Summers ◽  
William Neill ◽  
Patricia Dewar ◽  
Lorenzo Gonzalez ◽  
Renate van der Molen ◽  
...  
Keyword(s):  
Pulmonary Adenocarcinoma ◽  
Pokeweed Mitogen ◽  
Blood Leukocytes ◽  
Ovine Pulmonary Adenocarcinoma ◽  
Jaagsiekte Sheep Retrovirus ◽  
Adult Sheep ◽  
Specific Pathogen ◽  
Control Adult ◽  
Cellular Immune ◽  
And Control

Jaagsiekte sheep retrovirus (JSRV) is the aetiological agent of ovine pulmonary adenocarcinoma (OPA). To monitor changes in cellular immune function during JSRV infection, lymphoproliferation in response to various mitogens was measured in the blood of conventionally housed and specific-pathogen-free lambs experimentally infected with JSRV until the development of OPA and compared with uninfected control lambs. In addition, blood samples collected from adult field cases in the terminal stages of OPA and control adult sheep were compared. No difference in the proliferative response to phytohaemagglutinin and pokeweed mitogen between the animal groups was detected. In contrast, reduced responses to concanavalin A stimulation were demonstrated in the JSRV-inoculated lambs, prior to the onset of clinical disease, and also in the terminally ill adult sheep. Peripheral blood leukocytes were monitored to identify phenotypic frequency alterations. The CD4 lymphocytopaenia and neutrophilia reported previously in adult OPA cases were demonstrated but similar phenotypic changes were not identified during experimental infection.

Multiclonal pattern of Jaagsiekte sheep retrovirus integration sites in ovine pulmonary adenocarcinoma

2006 ◽  
Vol 117 (2) ◽  
pp. 254-263 ◽  
Author(s):  
Adrian W. Philbey ◽  
Christina Cousens ◽  
Jeanette V. Bishop ◽  
Clare A. Gill ◽  
James C. DeMartini ◽  
...  
Keyword(s):  
Pulmonary Adenocarcinoma ◽  
Ovine Pulmonary Adenocarcinoma ◽  
Jaagsiekte Sheep Retrovirus ◽  
Integration Sites ◽  
Retrovirus Integration
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