scholarly journals Whole genome analysis and characterization of Salmonella enterica subsp. enterica serovar Typhimurium strain UPM 260 for mediated genetic manipulation

2020 ◽  
Author(s):  
Najwa Syahirah Roslan ◽  
Nurulfiza Mat Isa ◽  
Abdul Rahman Omar ◽  
Mohd. Hair Bejo ◽  
Aini Ideris
Keyword(s):  
Salmonella Enterica ◽  
Functional Characterization ◽  
Poultry Meat ◽  
Whole Genome Sequence ◽  
Health Concern ◽  
Type I ◽  
Whole Genome ◽  
Typhimurium Strain ◽  
Serovar Typhimurium ◽  
The Impact

Abstract Background Salmonella enterica serovar Typhimurium persists as one of the most frequent food-borne zoonoses, causing a major public health concern worldwide. Furthermore, Salmonella infection has a large economic impact. Globally, the main sources of infection for humans include the consumption of contaminated poultry meat and eggs. In animals however, Salmonella transmission usually occurs horizontally from infected birds and contaminated environments. Hence, to delve further on how the impact of this disease can be lessened, an epidemiological study needs to be performed. It is vital to determine the genomic sequences of microorganisms to understand their biology and functional characterization. Thus, we determined the whole-genome sequence and virulence profile of S. enterica serovar Typhimurium strain UPM 260 isolated from Perak, Malaysia. Whole genome sequencing (WGS) using paired-end sequencing generated 107 contigs with a total genome size of 4.9 Mbp and 52% G+C content. The contigs were annotated for phylogenetic and functional analysis. Results Through the analysis, it is revealed that the genome were resistant to a number of antimicrobial drug classes including aminoglycoside, fluoroquinolone, tetracycline and phenicol. Also found in UPM 260 genome were three intact prophages (Fels-1, Gifsy-2 and one unique prophage, mEp390). The genome housed four types of restriction-modification systems (RMS) and Type I-E subtype of CRISPR-Cas system. Two metal resistance operons (mer and cop) and six pathogenicity islands (SPIs) were also discovered in UPM 260 genome. The SPIs contributed mostly to the bacterial virulence properties since 1054 CDS were reported to be homologous to the virulence factors in the database VFDB. Conclusion This study benefits us specifically in the field of genome engineering where gene-based genetic manipulations can be applied in reducing the prevalence and pathogenicity in Salmonella.

scholarly journals Whole-Genome Sequence of Salmonella enterica subsp. enterica Serovar Typhimurium Strain UPM 260, Isolated from a Broiler Chicken in Perak, Malaysia

2017 ◽  
Vol 5 (46) ◽  
Author(s):  
Najwa Syahirah Roslan ◽  
Shagufta Jabeen ◽  
Nurulfiza Mat Isa ◽  
Abdul Rahman Omar ◽  
Mohd Hair Bejo ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Salmonella Enterica ◽  
Broiler Chicken ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Content Type ◽  
Typhimurium Strain ◽  
Serovar Typhimurium

ABSTRACT Salmonella enterica subsp. enterica serovar Typhimurium is one of several well-categorized Salmonella serotypes recognized globally. Here, we report the whole-genome sequence of S. Typhimurium strain UPM 260, isolated from a broiler chicken.

scholarly journals Whole-Genome Sequence of Salmonella enterica subsp. enterica Serovar Typhimurium Strain WG49 and Escherichia coli Strain WG5 Used in South Africa for Phage Detection in Water Samples

2018 ◽  
Vol 6 (26) ◽  
Author(s):  
L. Bothma ◽  
D. Gonzalez-Ibeas ◽  
C. Mienie ◽  
C. C. Bezuidenhout ◽  
R. Adeleke
Keyword(s):  
Escherichia Coli ◽  
South Africa ◽  
Genome Sequence ◽  
Water Samples ◽  
Salmonella Enterica ◽  
Coli Strain ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Content Type ◽  
Serovar Typhimurium

Salmonella enterica subsp. enterica serovar Typhimurium WG49 is widely used for enumeration of F-specific RNA (F-RNA) coliphages in water.

scholarly journals Whole genome sequence analysis of NDM-1, CMY-4, and SHV-12 coproducing Salmonella enterica serovar Typhimurium isolated from a case of fatal burn wound infection

2018 ◽  
Vol Volume 11 ◽  
pp. 2491-2495 ◽  
Author(s):  
Kokila Banerjee ◽  
Priyadharshini Sekar ◽  
Padma Krishnan ◽  
Alice Wattam ◽  
Subhendu Roy ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Wound Infection ◽  
Genome Sequence ◽  
Salmonella Enterica ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Burn Wound ◽  
Genome Sequence Analysis ◽  
Serovar Typhimurium

scholarly journals Chlortetracycline Enhances Tonsil Colonization and Fecal Shedding of Multidrug-ResistantSalmonella entericaSerovar Typhimurium DT104 without Major Alterations to the Porcine Tonsillar and Intestinal Microbiota

2018 ◽  
Vol 85 (4) ◽  
Author(s):  
Devin B. Holman ◽  
Bradley L. Bearson ◽  
Heather K. Allen ◽  
Daniel C. Shippy ◽  
Crystal L. Loving ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
The United States ◽  
Multidrug Resistant ◽  
Foodborne Illness ◽  
Content Type ◽  
Fecal Shedding ◽  
Typhimurium Strain ◽  
Serovar Typhimurium ◽  
Collateral Effects ◽  
The Impact

ABSTRACTSalmonellaspp. are estimated to cause 1.2 million cases of human foodborne illness each year in the United States, and pigs can often be asymptomatically colonized withSalmonellaspp. (>50% of farms). Recent reports state that 18.3% ofSalmonella entericaserovar Typhimurium isolates are resistant to ≥3 antimicrobial classes, and multidrug-resistant (MDR) strains are associated with an increased hospitalization rate and other complications. Chlortetracycline is commonly used in swine production to prevent/treat various diseases; therefore, chlortetracycline treatment of pigs unknowingly colonized with MDRSalmonellamay have collateral effects onSalmonellaspp. (and other gut bacteria). In this study, we determined the effect of in-feed chlortetracycline (400 g/ton) on shedding and colonization of pigs challenged with the MDRS. Typhimurium strain DT104 (n= 11/group). We also assessed the impact on the fecal microbiota over the 12-day experimental period and on the ileum, cecum, and tonsil microbiota at 7 days postinoculation (dpi). In MDRS. Typhimurium-inoculated pigs, chlortetracycline administration significantly increased fecal shedding at 2 dpi (+1.4 log10CFU/g;P < 0.001) and enhanced tonsil colonization (+3.1 log10CFU/g;P < 0.001). There were few major alterations detected in the gut or tonsillar microbiota of pigs treated with MDRS. Typhimurium and/or chlortetracycline. The tonsillar transcriptome was largely unaffected despite increased colonization by MDRS. Typhimurium following inoculation of the chlortetracycline-treated pigs. These results highlight the idea that chlortetracycline administration can enhance shedding and colonization of MDRS. Typhimurium in pigs, which could increase the risk of environmental dissemination of MDRSalmonellastrains.IMPORTANCESalmonellaspp. are an important cause of foodborne illness in North America, and pork products are associated with sporadic cases and outbreaks of human salmonellosis. Isolates ofSalmonellamay be resistant to multiple antibiotics, and infections with multidrug-resistant (MDR)Salmonellaspp. are more difficult to treat, leading to increased hospitalization rates. Swine operations commonly use antimicrobials, such as chlortetracycline, to prevent/treat infections, which may have collateral effects on pig microbial populations. Recently, we demonstrated that chlortetracycline induces the expression of genes associated with pathogenesis and invasion in MDRSalmonella entericaserovar Typhimuriumin vitro. In our current study, we show increased tonsillar colonization and fecal shedding of the MDRS. Typhimurium strain DT104 from pigs administered chlortetracycline. Therefore, pigs unknowingly colonized with multidrug-resistantSalmonellaspp. and receiving chlortetracycline for an unrelated infection may be at a greater risk for disseminating MDRSalmonellaspp. to other pigs and to humans through environmental or pork product contamination.

scholarly journals Structural and Functional Characterization of ScsC, a Periplasmic Thioredoxin-Like Protein from Salmonella enterica Serovar Typhimurium

2013 ◽  
Vol 19 (13) ◽  
pp. 1494-1506 ◽  
Author(s):  
Mark Shepherd ◽  
Begoña Heras ◽  
Maud E. S. Achard ◽  
Gordon J. King ◽  
M. Pilar Argente ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Functional Characterization ◽  
Serovar Typhimurium

scholarly journals Characterization of a Laboratory-Derived, High-Level Ampicillin-Resistant Salmonella enterica Serovar Typhimurium Strain That Caused Meningitis in an Infant

2002 ◽  
Vol 46 (5) ◽  
pp. 1604-1606 ◽  
Author(s):  
Cheng-Hsun Chiu ◽  
Chishih Chu ◽  
Lin-Hui Su ◽  
Wan-Yu Wu ◽  
Tsu-Lan Wu
Keyword(s):  
Cerebrospinal Fluid ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Cloning Vector ◽  
Typhimurium Strain ◽  
Serovar Typhimurium ◽  
High Level ◽  
Lactamase Gene

ABSTRACT A Salmonella enterica serovar Typhimurium strain that harbored a plasmid carrying a TEM-1-type β-lactamase gene was isolated from the blood and cerebrospinal fluid of an infant with meningitis. This 3.2-kb plasmid was further characterized to be a nonconjugative pGEM series cloning vector containing a foreign insert. The strain was likely laboratory derived and contaminated the environment before it caused the infection.

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