EBV Viral Load in Tumor Cells Did Not Predict Tumor Extensiveness in Nasopharyngeal Cancer

Abstract Background:Nasopharyngeal cancer is commonly associated with EBV infection, especially the undifferentiated non keratinized histology. EBV DNA quantification through nasopharyngeal brushing was previously reported not related with disease stages. This study aimed to reinvestigate relationship of EBV viral load in tumor tissue with tumor extensiveness by more accurate EBV DNA quantification through microscopically confirmed tumor cells from nasopharyngeal biopsy. Method:The specimens for EBV DNA quantification was derived from histopathology slides which was pre-treated following QIAsymphony® SP Protocol for tissue DNA extraction. Then the extracted DNA underwent real time Polymerase Chain Reaction (RT-PCR) using artus® EBV RG PCR Kit for EBV DNA quantification. The tumor volume was determined by delineating gross tumor based on 3D imaging of the patient’s nasopharynx.Result:Twenty-four subjects were included in this study. All subjects were stage III and above with more males (75%) than females. EBV viral load in tumor cells were found to have no correlation with tumor volume both in local and nodal. The median local tumor volume was 81.3 cm3 ± 80 cm3. The median EBV viral load in tumor cells was 95,644.8 copies/100ng of DNA ± 224,758.4 copies/100ng of DNA. The median nodal or regional tumor volume was 35.7 cm3 ± 73.63 cm3.Conclusion:EBV viral load from tumor cells from nasopharyngeal biopsy has no relationship with tumor extensiveness in nasopharyngeal cancer. The presence and number of EBV in tumor cells did not translate into larger or smaller tumor. The EBV viral proteins and RNAs were perhaps more likely to confer some prognostic information due to the facts that those molecules were related with carcinogenesis.

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Epstein–Barr Virus (EBV) Viral Load in Tumor Cells Did Not Predict Tumor Extensiveness in Nasopharyngeal Cancer

Microbiology Research ◽

10.3390/microbiolres12010011 ◽

2021 ◽

Vol 12 (1) ◽

pp. 150-156

Author(s):

Soehartati A. Gondhowiardjo ◽

Handoko ◽

Marlinda Adham ◽

Lisnawati Rachmadi ◽

Henry Kodrat ◽

...

Keyword(s):

Viral Load ◽

Tumor Cells ◽

Epstein Barr Virus ◽

Tumor Volume ◽

Nasopharyngeal Cancer ◽

Dna Quantification ◽

Barr Virus ◽

Nasopharyngeal Biopsy ◽

Ebv Dna ◽

Epstein Barr

Background: Nasopharyngeal cancer is commonly associated with Epstein–Barr virus (EBV) infection, especially undifferentiated non-keratinized histology. EBV DNA quantification through nasopharyngeal brushing was previously reported to be not related to disease stage. This study aimed to reinvestigate the relationship of EBV viral load in tumor tissue with tumor extensiveness by more accurate EBV DNA quantification through microscopically confirmed tumor cells from nasopharyngeal biopsy. Method: The specimens for EBV DNA quantification were derived from histopathology slides which were pre-treated following the QIAsymphony® SP protocol for tissue DNA extraction. Then, the extracted DNA underwent real-time polymerase chain reaction (RT-PCR) using the artus® EBV RG PCR Kit for EBV DNA quantification. The tumor volume was determined by delineating the gross tumor based on 3D imaging of the patient’s nasopharynx. Result: Twenty-four subjects were included in this study. All subjects were stage III and above, with more males (75%) than females. EBV viral load in tumor cells was found to have no correlation to tumor volume both in local and nodal regions. The median local tumor volume was 81.3 cm3 ± 80 cm3. The median EBV viral load in tumor cells was 95,644.8 ± 224,758.4 copies/100 ng of DNA. The median nodal or regional tumor volume was 35.7 ± 73.63 cm3. Conclusion: EBV viral load from tumor cells from nasopharyngeal biopsy has no relationship with tumor extensiveness in nasopharyngeal cancer. The presence and amount of EBV in tumor cells did not translate into larger or smaller tumors. The EBV viral proteins and RNAs were perhaps more likely to confer some prognostic information due to the fact that those molecules were related to carcinogenesis.

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Is there a correlation between serum Epstein-Barr virus DNA level and tumor metabolic activity, TNM staging and tumor load in nasopharyngeal cancer patients?

Journal of Clinical Oncology ◽

10.1200/jco.2017.35.15_suppl.e17543 ◽

2017 ◽

Vol 35 (15_suppl) ◽

pp. e17543-e17543 ◽

Cited By ~ 1

Author(s):

Aysel Ahmedova ◽

Kubra Ozkaya ◽

Makbule Tambas ◽

Ugur Gezer ◽

Emre Ozgur ◽

...

Keyword(s):

Copy Number ◽

Epstein Barr Virus ◽

Tumor Volume ◽

Nasopharyngeal Cancer ◽

Significant Relation ◽

Dna Copy Number ◽

Barr Virus ◽

Pet Ct ◽

Ebv Dna ◽

Epstein Barr

e17543 Background: To investigate the relationship between pretreatment Epstein-Barr virus (EBV) DNA copy number and tumor metabolic activity, TNM stage and tumor volume in nasopharyngeal cancer (NPC) patients. Methods: Blood samples were collected 0-3 weeks before treatment and number of EBV DNA copies were determined by PCR . MRI and PET-CT were performed 0-4 weeks before treatment. The primary tumor volume (TVnp) and total volume of metastatic lymph nodes (TLV) were delineated separately on MRI by the same radiologist by the aid of program “Osirix” and recorded in cm3. Maximum SUV values of primary tumor (Tsuv) and metastatic lymph nodes (LNsuv) were determined with each PET-CT images. Kruskal Wallis test and Spearman's Correlation Analysis were used for the comparison of 3 or more groups with no normal distribution and the evaluation of the inter-parameter relations, respectively. Results: The study included 50 NPC patients treated between 2011 and 2015. There was no significant relation between serum EBV DNA copy number and the distribution of T stage (p = 0.81), N stage (p = 0.08), TVnp (r:0.009; p = 0.95), and Tsuv (r:-0.007, p = 0.96). However, a significant correlation was detected between EBV DNA copy number and both LNsuv (r:0.337; p = 0.017) and TLV (r:0.579; p = 0.001) (LNsuv and TLV increased as virus load increased). Conclusions: There is no significant relation between pretreatment EBV DNA load and T and N stages and TVnp in NPC patients. However, EBV DNA load has a significant correlation with TLV and LNsuv. These results may imply that TLV can be included in nodal staging and together with LNsuv be integrated in treatment planning.[Table: see text] [Table: see text]

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Comparison of Post-Treatment Plasma EBV DNA with Nasopharyngeal Biopsy in Patients after Radical (Chemo) Radiation Therapy for Non-metastatic Nasopharyngeal Cancer

International Journal of Radiation Oncology*Biology*Physics ◽

10.1016/j.ijrobp.2014.05.554 ◽

2014 ◽

Vol 90 (1) ◽

pp. S120

Author(s):

V.H. Lee ◽

D.L.W. Kwong ◽

K.O. Lam ◽

H.C.K. Sze ◽

P.P.Y. Ho ◽

...

Keyword(s):

Radiation Therapy ◽

Nasopharyngeal Cancer ◽

Post Treatment ◽

Nasopharyngeal Biopsy ◽

Ebv Dna

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Relationship of hepatocyte steatosis with viral load and genotype in chronic hepatitis C (CHC)

Journal of Hepatology ◽

10.1016/s0168-8278(01)81509-9 ◽

2001 ◽

Vol 34 ◽

pp. 173

Author(s):

M. Daimonakou ◽

I.K. Deladetsima ◽

N.C. Tassopoulos ◽

G. Karvountzis ◽

D. Tsantoulas ◽

...

Keyword(s):

Chronic Hepatitis ◽

Viral Load ◽

Hepatitis C ◽

Chronic Hepatitis C ◽

Hepatocyte Steatosis ◽

Relationship Of

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Dose-Effect Relationship of ZnPc-PDT on Tumor Cells In Vitro

Chinese Journal of Lasers ◽

10.3788/cjl201744.0307001 ◽

2017 ◽

Vol 44 (3) ◽

pp. 0307001

Author(s):

杨曼 Yang Man ◽

邢力允 Xing Liyun ◽

高卫栋 Gao Weidong ◽

顾月清 Gu Yueqing

Keyword(s):

Tumor Cells ◽

Dose Effect ◽

Effect Relationship ◽

Relationship Of

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Circulating tumor cells: a valuable marker of poor prognosis for advanced nasopharyngeal carcinoma

Molecular Medicine ◽

10.1186/s10020-019-0112-3 ◽

2019 ◽

Vol 25 (1) ◽

Cited By ~ 1

Author(s):

Guoping Ou ◽

Shan Xing ◽

Jianpei Li ◽

Lin Zhang ◽

Shulin Chen

Keyword(s):

Nasopharyngeal Carcinoma ◽

Tumor Cells ◽

Circulating Tumor Cells ◽

Roc Curve ◽

Poor Prognosis ◽

Regression Models ◽

Prognostic Value ◽

Stage Iv ◽

Stage Iii ◽

Ebv Dna

Abstract Purpose To evaluate the prognostic value of circulating tumor cells (CTCs) in nasopharyngeal carcinoma (NPC). Methods Cox’s proportional hazards regression models were used to identify whether CTCs was a poor prognostic factor for NPC. Chi-square tests were used to analyze and compare the distribution characteristics of CTCs in NPC. ROC curve was used to estimate the cut-off point of CTCs. Kaplan-Meier survival analyses were used to observe the prognostic value of CTCs alone and in combined with Epstein-Barr Virus DNA (EBV-DNA). Results CTCs was confirmed to be an independent risk factor for poor prognosis of NPC by Cox’s regression models that enrolled 370 NPC cases and took age, gender, EBV-DNA and CTCs as variables. The proportion of CTCs in stage IV NPC was statistically different from that in stage III; the cut-off point of CTCs between stage IV (288 cases) and stage III (70 cases) NPC estimated by ROC curve was 0.5. The prognosis of advanced NPC patients became worse with the increase of CTCs count. The combined detection of CTCs and EBV-DNA could better predict the prognosis of NPC compared with the single detection of EBV-DNA.

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Using Epstein-Barr Viral Load Assays To Diagnose, Monitor, and Prevent Posttransplant Lymphoproliferative Disorder

Clinical Microbiology Reviews ◽

10.1128/cmr.00006-09 ◽

2010 ◽

Vol 23 (2) ◽

pp. 350-366 ◽

Cited By ~ 151

Author(s):

Margaret L. Gulley ◽

Weihua Tang

Keyword(s):

Viral Load ◽

Lymphoproliferative Disorder ◽

Clinical Status ◽

Clinical Intervention ◽

Tumor Burden ◽

Posttransplant Lymphoproliferative Disorder ◽

Cell Immunity ◽

Ebv Dna ◽

Epstein Barr ◽

Diagnose Monitor

SUMMARYEpstein-Barr virus (EBV) DNA measurement is being incorporated into routine medical practice to help diagnose, monitor, and predict posttransplant lymphoproliferative disorder (PTLD) in immunocompromised graft recipients. PTLD is an aggressive neoplasm that almost always harbors EBV DNA within the neoplastic lymphocytes, and it is often fatal if not recognized and treated promptly. Validated protocols, commercial reagents, and automated instruments facilitate implementation of EBV load assays by real-time PCR. When applied to either whole blood or plasma, EBV DNA levels reflect clinical status with respect to EBV-related neoplasia. While many healthy transplant recipients have low viral loads, high EBV loads are strongly associated with current or impending PTLD. Complementary laboratory assays as well as histopathologic examination of lesional tissue help in interpreting modest elevations in viral load. Circulating EBV levels in serial samples reflect changes in tumor burden and represent an effective, noninvasive tool for monitoring the efficacy of therapy. In high-risk patients, serial testing permits early clinical intervention to prevent progression toward frank PTLD. Restoring T cell immunity against EBV is a major strategy for overcoming PTLD, and novel EBV-directed therapies are being explored to thwart virus-driven neoplasia.

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FAMILIAL MAMMARY TUMORS IN THE RABBIT

Journal of Experimental Medicine ◽

10.1084/jem.71.3.305 ◽

1940 ◽

Vol 71 (3) ◽

pp. 305-324 ◽

Cited By ~ 34

Author(s):

Harry S. N. Greene

Keyword(s):

Tumor Cells ◽

Developmental Stages ◽

Mammary Tumors ◽

Serial Transfer ◽

Local Tissue ◽

Tissue Invasion ◽

Series Of Experiments ◽

Papillary Type ◽

Relationship Of ◽

Morphological Relationship

A series of experiments is described in which fragments derived from two mammary tumors of distinct types were transferred at different developmental stages to the anterior chamber of the eye of normal rabbits. It was found that the ability to survive and to grow progressively after transplantation was not immediately related to anaplastic cellular changes. On the other hand, there existed a definite correlation between the success of transplantation and the morphological relationship of tumor cells and the normal cells of the host. Transplantation to normal animals could not be effected during stages of local tissue invasion but was successfully performed as soon as the tumor cells manifested the ability to invade foreign tissues or to metastasize in the spontaneous host. It was concluded, therefore, that neither anaplasia nor local tissue invasion represented autonomy but, rather, stages in its development and that the final attainment of this condition was only evidenced by metastasis or by invasion in foreign tissues. The tumors were successfully transplanted to normal animals during this series of experiments and have been carried by serial transfer to the present time. The most outstanding feature in the transplantation of the papillary type tumor was the marked difference in susceptibility exhibited by the two sexes. The acinar type tumor was distinguished by high transplantability, an extremely rapid growth rate and early regression.

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