scholarly journals Biorefinery Cascade Processing For Converting Corncob To Xylooligosaccharides And Glucose By Maleic Acid Pretreatment

2021 ◽  
Author(s):  
Qibo Zhang ◽  
Ali Nawaz ◽  
Kankan Jiang ◽  
Xin Zhou ◽  
Yong Xu
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Maleic Acid ◽  
Low Cost ◽  
Value Added ◽  
The Other ◽  
Acid Pretreatment ◽  
Experimental Procedure ◽  
Solids Loading ◽  
Novel Strategy

Abstract Corncob as an abundance and low-cost waste resource has received increasing attention to produce value added chemicals, it is rich in xylan and regarded as the most preferable feedstock for preparing xylooligosaccharides, which possesses highly commercial value due to a range of health benefits. The strategy with xylooligosaccharides as core products can cut costs and improve economic efficiency of biorefinery. Therefore, a cascade processing for converting corncob to xylooligosaccharides and glucose by sequential maleic acid pretreatment and enzymatic hydrolysis was design. Based on overarching aim of this study that maximally yielding xylooligosaccharides, corncob was first subjected to response surface methodology experimental procedure for optimizing the conditions. Correspondingly, a maximum xylooligosaccharides yield of 52.9% was achieved with 0.5% maleic acid at 155 °C for 26 min. Maleic acid, a non-toxic and edible catalyst, was able to effectively hydrolyze xylan into xylooligosaccharides and simultaneously generate a positive pretreated effect for improve the enzymatic hydrolysis efficiency. Finally, an enzymatic hydrolysis yield of 87.5% was achieved from maleic acid-treated corncob at 10% solids loading. This cascade processing may provide a novel strategy for the other biomass wastes utilization.

scholarly journals Peningkatan Produksi Gula Pereduksi dari Tandan Kosong Kelapa Sawit dengan Praperlakuan Asam Organik pada Reaktor Bertekanan

REAKTOR ◽  
2017 ◽  
Vol 16 (4) ◽  
pp. 199
Author(s):  
Fahriya Puspita Sari ◽  
Nissa Nurfajrin Solihat ◽  
Sita Heris Anita ◽  
Fitria Fitria ◽  
Euis Hermiati
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oxalic Acid ◽  
Organic Acid ◽  
Oil Palm ◽  
Maleic Acid ◽  
Reducing Sugar ◽  
Sugar Production ◽  
Empty Fruit Bunch ◽  
Acid Pretreatment ◽  
Severity Factor

ENHANCEMENT OF REDUCING SUGAR PRODUCTION FROM OIL PALM EMPTY FRUIT BUNCH BY PRETREATMENT USING ORGANIC ACID IN PRESSURIZED REACTOR. Organic acids are potential to create more environmentally friendly process in the pretreatment of lignocellulosic biomass for bioethanol production. This study was aimed to investigate the influence of organic acid pretreatment in reducing sugar production in a pressurized reactor with various resident times and temperatures on enzymatic hydrolysis of OPEFB. Two different organic acids (maleic acid and oxalic acid) were used in the pretreatment of oil palm empty fruit bunch (OPEFB) using a pressurized reactor. Factorial design using three different temperatures (170, 180, and 190°C) and four resident times (15, 30, 45, and 60 min) were employed, followed by enzymatic hydrolysis. Each condition conducted two repetitions. Analysis was conducted on the reducing sugar that was produced after saccharification by means of the severity factor of each pretreatment condition. Maleic acid showed higher reducing sugar yield with lower severity factor than oxalic acid with the same operating conditions. The highest yield of reducing sugars (80.84%) was obtained using maleic acid at 170 for 60 minutes with severity factor of 1.836. Keywords: bioethanol; organic acid pretreatment; pressurized reactor; severity factor; oil palm empty fruit bunches;   Abstrak Asam organik berpotensi dalam membantu proses praperlakuan dari biomassa lignoselulosa untuk memproduksi bioetanol yang ramah lingkungan. Penelitian ini bertujuan untuk mengetahui pengaruh asam organik, suhu dan waktu operasi terhadap produksi gula pereduksi dengan reaktor bertekanan pada tandan kosong kelapa sawit. Dua asam organik yang berbeda yaitu asam oksalat dan asam maleat digunakan untuk proses praperlakuan tandan kosong kelapa sawit (TKKS) dengan bantuan reaktor bertekanan. Dalam proses praperlakuan digunakan tiga suhu yang berbeda yaitu suhu 170, 180, dan 190°C dan empat waktu operasi 15, 30, 45, dan 60 min yang dilanjutkan dengan proses hidrolisis enzimatis. Setiap kondisi dilakukan dua kali pengulangan. Analisa yang digunakan adalah analisa uji gula pereduksi dan severity factor pada kondisi tiap praperlakuan. Asam maleat menunjukkan hasil yang lebih baik dengan severity factor yang lebih rendah dibandingkan menggunakan asam oksalat dengan kondisi operasi yang sama. Hasil yang didapatkan menunjukkan bahwa praperlakuan tandan kosong kelapa sawit dengan bantuan reaktor bertekanan memiliki rendemen gula pereduksi optimum sebesar 80,84% dengan menggunakan asam maleat pada suhu 170°C selama 60 menit dengan severity factor sebesar 1,836. Kata kunci: bioetanol; praperlakuan asam organik; reaktor bertekanan; severity factor; tandan kosong kelapa sawit.

scholarly journals Evaluation of the Interactive Effect Pretreatment Parameters via Three Types of Microwave-Assisted Pretreatment and Enzymatic Hydrolysis on Sugar Yield

Processes ◽  
2020 ◽  
Vol 8 (7) ◽  
pp. 787 ◽  
Author(s):  
Saleem Ethaib ◽  
Rozita Omar ◽  
Mustapa Kamal Siti Mazlina ◽  
Awang Biak Dayang Radiah
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Interactive Effect ◽  
Reducing Sugar ◽  
Sugar Yield ◽  
Solid Loading ◽  
Acid Pretreatment ◽  
Glucose Yield ◽  
Sago Palm ◽  
Microwave Assisted

This study aims to evaluate the sugar yield from enzymatic hydrolysis and the interactive effect pretreatment parameters of microwave-assisted pretreatment on glucose and xylose. Three types of microwave-assisted pretreatments of sago palm bark (SPB) were conducted for enzymatic hydrolysis, namely: microwave-sulphuric acid pretreatment (MSA), microwave-sodium hydroxide pretreatment (MSH), and microwave-sodium bicarbonate (MSB). The experimental design was done using a response surface methodology (RSM) and Box–Behenken Design (BBD). The pretreatment parameters ranged from 5–15% solid loading (SL), 5–15 min of exposure time (ET), and 80–800 W of microwave power (MP). The results indicated that the maximum total reducing sugar was 386 mg/g, obtained by MSA pretreatment. The results also illustrated that the higher glucose yield, 44.3 mg/g, was found using MSH pretreatment, while the higher xylose yield, 43.1 mg/g, resulted from MSA pretreatment. The pretreatment parameters MP, ET, and SL showed different patterns of influence on glucose and xylose yield via enzymatic hydrolysis for MSA, MSH, and MSB pretreatments. The analyses of the interactive effect of the pretreatment parameters MP, ET, and SL on the glucose yield from SPB showed that it increased with the high MP and longer ET, but this was limited by low SL values. However, the analysis of the interactive effect of the pretreatment parameters on xylose yields revealed that MP had the most influence on the xylose yield for MSA, MSH, and MSB pretreatments.

scholarly journals Utilization of Agricultural Waste for the Production of Xylooligosaccharides Using Response Surface Methodology and Their In Vitro Prebiotic Efficacy

2021 ◽  
Author(s):  
Nagamani Kathiresan ◽  
Lingesh Gopal ◽  
Vijay Karuppiah ◽  
Renuka Naveenethan ◽  
David Ravindran Abraham ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Response Surface ◽  
Sugarcane Bagasse ◽  
Agricultural Waste ◽  
Relative Yield ◽  
Value Added ◽  
A Value ◽  
Hydrolysis Of

Abstract Air pollution is a prominent problem recently faced in various parts of India due to the burning of stubbles (coconut husk, corn cob, paddy stubbles, sugarcane bagasse, etc.) which are rich in a lignocellulosic component that can be converted into a prebiotic known as Xylooliogsaccaride (XOS). They can be produced by autohydrolysis, acid hydrolysis and enzymatic hydrolysis of xylan. In the present study, Xylan was extracted from sugarcane bagasse using two alkalis (NaOH and KOH) and the yield was compared. Xylooligosaccharide produced by enzymatic hydrolysis and their factors influencing the yield were optimized using Response Surface Methodology. Xylan and Xylooligosaccharide was characterized by FTIR, NMR, XRD, TGA and ESI-MS. Xylooligosaccharides was investigated for their prebiotic potential by in vitro study. The maximum (Relative yield of 86%) yield of xylan was observed in 20% of NaOH. Xylan peaks at 3762cm− 1, 3347 cm− 1, 2917cm− 1 represents the OH and CH stretching of xylan. The main signals at 4.26 (H-1), 3.19 (H-2), 3.59 (H-3), 3.63 (H-4) and 3.98 (H-5) ppm determines the existence of xylan. The higher amount of XOS is pH 4.75, temperature 45°C, enzyme 4U/ml and for time of 16h. The spectrum of 5.0-5.40ppm and 4.30-4.60ppm represents the α anomeric and β anomeric protons in XOS. They are resistant digested and the reaching percentage to the intestine is 95% unhydrolyzed. The maximum prebiotic index was noted in L.plantarum (1.92) and L.fermentum (1.61). The highest prebiotic index and score was observed in L.plantarum (1.9) and L.fermentum (17). The maximum bacteriocin production of Enterococcus faecium against E.fecalis (13mm) and Streptococcus pyogenes (11mm). Therefore, utilization of agricultural residues for a value-added product not only shows a great impact on environmental issues but also could double the farmer’s income

Optimization of acid pretreatment and enzymatic hydrolysis on the production of ethanol fuel from waste banana peels

2018 ◽  
Vol 29 (8) ◽  
pp. 1354-1364 ◽  
Author(s):  
Jiaxin Guo ◽  
Zhenchi Li ◽  
Lingcheng Su ◽  
Yiu-Fai Tsang ◽  
AK An ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Tartaric Acid ◽  
Value Added ◽  
Banana Peel ◽  
Orthogonal Experimental Design ◽  
Yeast Fermentation ◽  
Banana Fruit ◽  
Optimal Conditions ◽  
Ethanol Fuel ◽  
Acid Pretreatment

This study investigated the application of waste banana peels as feedstock for the production of ethanol fuel as a second-generation biofuel. First, acid pretreatment followed by enzymatic hydrolysis converted both the cellulosic and hemicellulosic biomass of the peels into fermentable sugars, which eventually produced ethanol by yeast fermentation. The optimal conditions for the production of ethanol fuel were determined by orthogonal experimental design method. The results showed that 100 g of fresh banana would produce 31.4 g of banana peel which could be turned into 2.8 g dried peel powder. Under optimal conditions of acid pretreatment with 0.2% tartaric acid, enzymatic hydrolysis by cellulase and yeast fermentation, 115 mg of ethanol (95% purity) could be recovered by distillation from the fermentation broth, which was approximately 4% by weight of the dried peel powder. This study concluded that banana fruit, after the removal of peels for consumption or food processing, the discarded peels will no longer pose an organic waste problem to the environment if they could be recollected and converted into value-added products like ethanol fuel. We also demonstrated that tartaric acid, an organic acid used in the acid pretreatment, for the first time to our knowledge, outperformed the traditional sulfuric acid used commonly in other studies.

scholarly journals Sequential Hydrothermal HCl Pretreatment and Enzymatic Hydrolysis of Saccharina japonica Biomass

Energies ◽  
2021 ◽  
Vol 14 (23) ◽  
pp. 8053
Author(s):  
Eun-Young Park ◽  
Jung-Kyu Park
Keyword(s):  
Enzymatic Hydrolysis ◽  
Saccharina Japonica ◽  
Reducing Sugar ◽  
Single Treatment ◽  
Acid Pretreatment ◽  
Experimental Procedure ◽  
Independent Variables ◽  
Hcl Concentration ◽  
Hydrolysis Of ◽  
Temperature Time

This study investigated the production of fermentable sugars from carbohydrate-rich macroalgae Saccharina japonica using sequential hydrolysis (hydrothermal acid pretreatment and enzymatic hydrolysis) to determine the maximum reducing sugar yield (RSy). The sequential hydrolysis was predicted by three independent variables (temperature, time, and HCl concentration) using response surface methodology (RSM). Enzymatic hydrolysis (8.17% v/wbiomass Celluclast® 1.5 L, 26.4 h, 42.6 °C) was performed after hydrothermal acid pretreatment under predicted conditions (143.6 °C, 22 min, and 0.108 N HCl concentration). Using this experimental procedure, the yields of hydrothermal acid pretreatment, enzymatic hydrolysis, and sequential hydrolysis were 115.6 ± 0.4 mg/g, 117.7 ± 0.3 mg/g, and 183.5 ± 0.6 mg/g, respectively. Our results suggested that sequential hydrolysis of hydrothermal acid pretreatment and enzymatic hydrolysis was more efficient than their single treatment.

scholarly journals Utilization of Agricultural Waste for the Production of Xylooligosaccharides Using Response Surface Methodology and Their In Vitro Prebiotic Efficacy

2021 ◽  
Author(s):  
Nagamani Kathiresan ◽  
Lingesh Gopal ◽  
Renuka Naveenathan ◽  
Vijay Karuppiah ◽  
David Ravindran Abraham ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Response Surface ◽  
Sugarcane Bagasse ◽  
Agricultural Waste ◽  
Relative Yield ◽  
Value Added ◽  
A Value ◽  
Hydrolysis Of

Abstract Air pollution is a prominent problem recently faced in various parts of India due to the burning of stubbles (coconut husk, corn cob, paddy stubbles, sugarcane bagasse, etc.) which are rich in a lignocellulosic component that can be converted into a prebiotic known as Xylooliogsaccaride (XOS). They can be produced by autohydrolysis, acid hydrolysis and enzymatic hydrolysis of xylan. In the present study, Xylan was extracted from sugarcane bagasse using two alkalis (NaOH and KOH) and the yield was compared. Xylooligosaccharide produced by enzymatic hydrolysis and their factors influencing the yield were optimized using Response Surface Methodology. Xylan and Xylooligosaccharide was characterized by FTIR, NMR, XRD, TGA and ESI-MS. Xylooligosaccharides was investigated for their prebiotic potential by in vitro study. The maximum (Relative yield of 86%) yield of xylan was observed in 20% of NaOH. Xylan peaks at 3762cm-1, 3347 cm-1, 2917cm-1 represents the OH and CH stretching of xylan. The main signals at 4.26 (H-1), 3.19 (H-2), 3.59 (H-3), 3.63 (H-4) and 3.98 (H-5) ppm determines the existence of xylan. The higher amount of XOS is pH 4.75, temperature 45°C, enzyme 4U/ml and for time of 16h. The spectrum of 5.0-5.40ppm and 4.30-4.60ppm represents the α anomeric and β anomeric protons in XOS. They are resistant digested and the reaching percentage to the intestine is 95% unhydrolyzed. The maximum prebiotic index was noted in L.plantarum (1.92) and L.fermentum (1.61). The highest prebiotic index and score was observed in L.plantarum (1.9) and L.fermentum (17). The maximum bacteriocin production of Enterococcus faecium against E.fecalis (13mm) and Streptococcus pyogenes (11mm). Therefore, utilization of agricultural residues for a value-added product not only shows a great impact on environmental issues but also could double the farmer’s income

scholarly journals Revalorization of sunflower stalk pith as feedstock for the coproduction of pectin and glucose using a two-step dilute acid pretreatment process

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Qibo Zhang ◽  
Lu Cheng ◽  
Xutong Ma ◽  
Xin Zhou ◽  
Yong Xu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Treatment Process ◽  
Value Added ◽  
Raw Material ◽  
Dilute Acid Pretreatment ◽  
Solid Loading ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Hydrolysis Method ◽  
Sulfuric Acid Treatment

Abstract Background Sunflower stalk pith, residue from the processing of sunflower, is rich in pectin and cellulose, thereby acting as an economic raw material for the acquisition of these compounds. In order to increase the commercial value of sunflower processing industry, a two-step dilute sulfuric acid treatment process was conducted on spent sunflower stalk pith to obtain the value-added products, pectin and glucose. Results In this study, pectin was firstly extracted under mild acid condition to avoid pectin degradation, which was conducted at 90 °C with a pH of 2.0 for 2 h, and ~0.14 g/g of pectin could be recovered. Then the remaining solids after pectin extraction were subjected to the reinforced treatment process with 0.75% H2SO4 at 150 °C for 30 min to further improve enzymatic hydrolysis efficiency. Moreover, by combining a fed-batch enzymatic hydrolysis strategy, a solid loading content of 16% was successfully achieved and the glucose titer reached 103.1 g/L with a yield of 83.6%. Conclusion Finally, ~140 g pectin and 260 g glucose were produced from 1 kg of raw sunflower stalk pith using the integrated biorefinery process. This work puts forward a two-step dilute acid pretreatment combined with enzymatic hydrolysis method to produce pectin and glucose from sunflower spent waste.

Dilute acid pretreatment of rice straw, structural characterization and optimization of enzymatic hydrolysis conditions by response surface methodology

RSC Advances ◽  
2015 ◽  
Vol 5 (58) ◽  
pp. 46525-46533 ◽  
Author(s):  
Siddheshwar Dnyandev Kshirsagar ◽  
Pankajkumar Ramdas Waghmare ◽  
Prakash Chandrakant Loni ◽  
Sushama Anandrao Patil ◽  
Sanjay Prabhu Govindwar
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Production Technology ◽  
Structural Characterization ◽  
Dilute Acid Pretreatment ◽  
Fermentable Sugars ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Hydrolysis Conditions ◽  
A Current

Efficient conversion of fermentable sugars from cheap lignocellulosic biomass is a current need in viable ethanol production technology.

Revalorization of Sunflower Stalk Pitch As Feedstock for the Coproduction of Pectin and Glucose Using a Two-Step Dilute Acid Pretreatment Process

2021 ◽  
Author(s):  
Qibo Zhang ◽  
Xutong Ma ◽  
Xin Zhou ◽  
Yong Xu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Value Added ◽  
Raw Material ◽  
Dilute Acid Pretreatment ◽  
Dilute Acid ◽  
Pectin Degradation ◽  
Acid Pretreatment ◽  
Hydrolysis Method ◽  
Sulfuric Acid Treatment ◽  
Value Added Products

Abstract Background: Sunflower stalk pith, residue from the processing of sunflower, is rich in pectin and cellulose, thereby acting as an economic raw material for the acquisition of these compounds. In order to increase the commercial value of sunflower processing industry, a two-step sequential dilute sulfuric acid treatment combined with subsequent enzymatic hydrolysis was conducted on spent sunflower stalk pith to obtain the value-added products, pectin and glucose. Results: In this study, pectin was firstly extracted with a mild condition to avoid pectin degradation, which conducted at 95℃ with a pH of 2.0 for 2 h, and approximately 0.12 g/g of pectin could be recovered. Then the remaining solids followed by extracted pectin were subjected to the reinforced treatment process with 0.75% H2SO4 at 150 oC for 30 min to further improving enzymatic hydrolysis efficiency. Moreover, a fed-batch enzymatic hydrolysis was successfully performed with a solid 16% content, the glucose titer reached 103.1 g/L with a yield of 83.6 %.Conclusion: Finally, approximately 140 g pectin and 260 g glucose were produced from 1 kg of raw sunflower stalk pith using the integrated biorefinery process. This work put forward a two-step dilute acid pretreatment combined with enzymatic hydrolysis method to produce pectin and glucose from sunflower spent waste.

Sign in / Sign up

Export Citation Format

Share Document