scholarly journals Evaluation of the Interactive Effect Pretreatment Parameters via Three Types of Microwave-Assisted Pretreatment and Enzymatic Hydrolysis on Sugar Yield

Processes ◽  
2020 ◽  
Vol 8 (7) ◽  
pp. 787 ◽  
Author(s):  
Saleem Ethaib ◽  
Rozita Omar ◽  
Mustapa Kamal Siti Mazlina ◽  
Awang Biak Dayang Radiah
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Interactive Effect ◽  
Reducing Sugar ◽  
Sugar Yield ◽  
Solid Loading ◽  
Acid Pretreatment ◽  
Glucose Yield ◽  
Sago Palm ◽  
Microwave Assisted

This study aims to evaluate the sugar yield from enzymatic hydrolysis and the interactive effect pretreatment parameters of microwave-assisted pretreatment on glucose and xylose. Three types of microwave-assisted pretreatments of sago palm bark (SPB) were conducted for enzymatic hydrolysis, namely: microwave-sulphuric acid pretreatment (MSA), microwave-sodium hydroxide pretreatment (MSH), and microwave-sodium bicarbonate (MSB). The experimental design was done using a response surface methodology (RSM) and Box–Behenken Design (BBD). The pretreatment parameters ranged from 5–15% solid loading (SL), 5–15 min of exposure time (ET), and 80–800 W of microwave power (MP). The results indicated that the maximum total reducing sugar was 386 mg/g, obtained by MSA pretreatment. The results also illustrated that the higher glucose yield, 44.3 mg/g, was found using MSH pretreatment, while the higher xylose yield, 43.1 mg/g, resulted from MSA pretreatment. The pretreatment parameters MP, ET, and SL showed different patterns of influence on glucose and xylose yield via enzymatic hydrolysis for MSA, MSH, and MSB pretreatments. The analyses of the interactive effect of the pretreatment parameters MP, ET, and SL on the glucose yield from SPB showed that it increased with the high MP and longer ET, but this was limited by low SL values. However, the analysis of the interactive effect of the pretreatment parameters on xylose yields revealed that MP had the most influence on the xylose yield for MSA, MSH, and MSB pretreatments.

scholarly journals Microwave-assisted Dilute Acid Pretreatment and Enzymatic Hydrolysis of Sago Palm Bark

BioResources ◽  
2016 ◽  
Vol 11 (3) ◽  
Author(s):  
Saleem Ethaib ◽  
Rozita Omar ◽  
Mustapa Kamal Siti Mazlina ◽  
Awang Biak Dayang Radiah ◽  
Syams Syafiie
Keyword(s):  
Enzymatic Hydrolysis ◽  
Dilute Acid Pretreatment ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Sago Palm ◽  
Microwave Assisted ◽  
Hydrolysis Of

Acid-Alkaline Two-Stage Pretreatments of Corn Stover for Enhancing Enzymatic Saccharification

2013 ◽  
Vol 724-725 ◽  
pp. 207-211 ◽  
Author(s):  
Hai Song Wang ◽  
Hong Ling Gao ◽  
Bin Li ◽  
Xin Dong Mu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Corn Stover ◽  
Lignocellulosic Biomass ◽  
Enzymatic Saccharification ◽  
Reducing Sugar ◽  
Sugar Yield ◽  
Alkaline Pretreatment ◽  
Two Stage ◽  
Glucose Yield ◽  
Second Stage

For the enzymatic saccharification of lignocellulosic biomass, single acid or alkaline pretreatment is not satisfactory because of the low sugar yields together with the neutralization of residual chemicals before enzymatic hydrolysis. Herein, an acid-alkaline two-stage pretreatment process was designed to treat corn stover. During the process, the pretreated liquid from the first stage and the solid residues from the second stage were mixed together for the subsequent simultaneous enzymatic hydrolysis, where a mixture of cellulase with an activity loading of 20 FPU/g substrate, cellobiase with an activity loading of 5 U/g substrate, and xylanase with an activity loading of 200 U/g substrate was used. Compared to the single acid or alkaline pretreatment, the acid-alkaline two-stage pretreatment could significantly improve the enzymatic saccharification, and 91.2% glucose yield with 52.56% of the theoretical total reducing sugar yield was achieved after the subsequent enzymatic hydrolysis.

scholarly journals A novel recyclable furoic acid-assisted pretreatment for sugarcane bagasse biorefinery in co-production of xylooligosaccharides and glucose

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Lin Dai ◽  
Tian Huang ◽  
Kankan Jiang ◽  
Xin Zhou ◽  
Yong Xu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Production Cost ◽  
Solid Loading ◽  
Fed Batch ◽  
Acid Pretreatment ◽  
Furoic Acid ◽  
Cooling Crystallization ◽  
Mineral Acids ◽  
Hydrolysis Of

Abstract Background Pretreatment is the key step for utilizing lignocellulosic biomass, which can extract cellulose from lignin and disrupt its recalcitrant crystalline structure to allow much more effective enzymatic hydrolysis; and organic acids pretreatment with dual benefic for generating xylooligosaccharides and boosting enzymatic hydrolysis has been widely used in adding values to lignocellulose materials. In this work, furoic acid, a novel recyclable organic acid as catalyst, was employed to pretreat sugarcane bagasse to recover the xylooligosaccharides fraction from hemicellulose and boost the subsequent cellulose saccharification. Results The FA-assisted hydrolysis of sugarcane bagasse using 3% furoic acid at 170 °C for 15 min resulted in the highest xylooligosaccharides yield of 45.6%; subsequently, 83.1 g/L of glucose was harvested by a fed-batch operation with a solid loading of 15%. Overall, a total of 120 g of xylooligosaccharides and 335 g glucose could be collected from 1000 g sugarcane bagasse starting from the furoic acid pretreatment. Furthermore, furoic acid can be easily recovered by cooling crystallization. Conclusion This work put forward a novel furoic acid pretreatment method to convert sugarcane bagasse into xylooligosaccharides and glucose, which provides a strategy that the sugar and nutraceutical industries can be used to reduce the production cost. The developed process showed that the yields of xylooligosaccharides and byproducts were controllable by shortening the reaction time; meanwhile, the recyclability of furoic acid also can potentially reduce the pretreatment cost and potentially replace the traditional mineral acids pretreatment.

Optimization of Fermentable Sugar Production from Pineapple Leaf Waste (Ananas comosus [L.] Merr) by Enzymatic Hydrolysis

2021 ◽  
Vol 18 (1) ◽  
pp. 73-80
Author(s):  
Yohanita Restu Widihastuty ◽  
Sutini Sutini ◽  
Aida Nur Ramadhani
Keyword(s):  
Enzymatic Hydrolysis ◽  
Complex Structure ◽  
Reducing Sugar ◽  
Sugar Yield ◽  
Ananas Comosus ◽  
Optimum Operating ◽  
Cellulose Content ◽  
Optimum Operating Condition ◽  
Operating Condition ◽  
Cellulase Enzyme

Pineapple leaf waste is one agricultural waste that has high cellulose content. Pineapple leaf waste's complex structure contains a bundle of packed fiber that makes it hard to remove lignin and hemicellulose structure, so challenging to produce reducing sugar. Dried pineapple leaf waste pretreated with a grinder to break its complex structure. Delignification process using 2% w/v NaOH solution at 87oC for 60 minutes has been carried out to remove lignin and hemicellulose structure so reducing sugar could be produced. Delignified pineapple leaf waste has been enzymatic hydrolyzed using cellulase enzyme (6 mL, 7 mL, and 8 mL) to produce reducing sugar. The sample was incubated in an incubator shaker at 155 rpm at 45, 55, and 60oC for 72 hours. Determination of reducing sugar yield had been carried out using the Dubois method and HPLC. The model indicated that the optimum operating condition of enzymatic hydrolysis is 7 mL of cellulase enzyme at 55oC to produce 96,673 mg/L reducing sugar. This result indicated that the enzymatic hydrolysis operating condition improved the reducing sugar yield from pineapple leaf waste. The optimum reducing sugar yield can produce biofuel by the saccharification process.

scholarly journals Peningkatan Produksi Gula Pereduksi dari Tandan Kosong Kelapa Sawit dengan Praperlakuan Asam Organik pada Reaktor Bertekanan

REAKTOR ◽  
2017 ◽  
Vol 16 (4) ◽  
pp. 199
Author(s):  
Fahriya Puspita Sari ◽  
Nissa Nurfajrin Solihat ◽  
Sita Heris Anita ◽  
Fitria Fitria ◽  
Euis Hermiati
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oxalic Acid ◽  
Organic Acid ◽  
Oil Palm ◽  
Maleic Acid ◽  
Reducing Sugar ◽  
Sugar Production ◽  
Empty Fruit Bunch ◽  
Acid Pretreatment ◽  
Severity Factor

ENHANCEMENT OF REDUCING SUGAR PRODUCTION FROM OIL PALM EMPTY FRUIT BUNCH BY PRETREATMENT USING ORGANIC ACID IN PRESSURIZED REACTOR. Organic acids are potential to create more environmentally friendly process in the pretreatment of lignocellulosic biomass for bioethanol production. This study was aimed to investigate the influence of organic acid pretreatment in reducing sugar production in a pressurized reactor with various resident times and temperatures on enzymatic hydrolysis of OPEFB. Two different organic acids (maleic acid and oxalic acid) were used in the pretreatment of oil palm empty fruit bunch (OPEFB) using a pressurized reactor. Factorial design using three different temperatures (170, 180, and 190°C) and four resident times (15, 30, 45, and 60 min) were employed, followed by enzymatic hydrolysis. Each condition conducted two repetitions. Analysis was conducted on the reducing sugar that was produced after saccharification by means of the severity factor of each pretreatment condition. Maleic acid showed higher reducing sugar yield with lower severity factor than oxalic acid with the same operating conditions. The highest yield of reducing sugars (80.84%) was obtained using maleic acid at 170 for 60 minutes with severity factor of 1.836. Keywords: bioethanol; organic acid pretreatment; pressurized reactor; severity factor; oil palm empty fruit bunches;   Abstrak Asam organik berpotensi dalam membantu proses praperlakuan dari biomassa lignoselulosa untuk memproduksi bioetanol yang ramah lingkungan. Penelitian ini bertujuan untuk mengetahui pengaruh asam organik, suhu dan waktu operasi terhadap produksi gula pereduksi dengan reaktor bertekanan pada tandan kosong kelapa sawit. Dua asam organik yang berbeda yaitu asam oksalat dan asam maleat digunakan untuk proses praperlakuan tandan kosong kelapa sawit (TKKS) dengan bantuan reaktor bertekanan. Dalam proses praperlakuan digunakan tiga suhu yang berbeda yaitu suhu 170, 180, dan 190°C dan empat waktu operasi 15, 30, 45, dan 60 min yang dilanjutkan dengan proses hidrolisis enzimatis. Setiap kondisi dilakukan dua kali pengulangan. Analisa yang digunakan adalah analisa uji gula pereduksi dan severity factor pada kondisi tiap praperlakuan. Asam maleat menunjukkan hasil yang lebih baik dengan severity factor yang lebih rendah dibandingkan menggunakan asam oksalat dengan kondisi operasi yang sama. Hasil yang didapatkan menunjukkan bahwa praperlakuan tandan kosong kelapa sawit dengan bantuan reaktor bertekanan memiliki rendemen gula pereduksi optimum sebesar 80,84% dengan menggunakan asam maleat pada suhu 170°C selama 60 menit dengan severity factor sebesar 1,836. Kata kunci: bioetanol; praperlakuan asam organik; reaktor bertekanan; severity factor; tandan kosong kelapa sawit.

scholarly journals A novel recyclable furoic acid-assisted pretreatment for sugarcane bagasse biorefinery in co-production of xylooligosaccharides and glucose

2020 ◽  
Author(s):  
Lin Dai ◽  
Tian Huang ◽  
Kankan Jiang ◽  
Xin Zhou ◽  
Yong Xu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Production Cost ◽  
Solid Loading ◽  
Fed Batch ◽  
Acid Pretreatment ◽  
Furoic Acid ◽  
Cooling Crystallization ◽  
Mineral Acids ◽  
Hydrolysis Of

Abstract Background: Pretreatment is the key step for utilizing lignocellulosic biomass, which can extract cellulose from lignin and disrupt its recalcitrant crystalline structure to allow much more effective enzymatic hydrolysis; and organic acids pretreatment with dual benefic for generating xylooligosaccharides and boosting enzymatic hydrolysis has been widely used in adding values to lignocellulose materials. In this work, furoic acid, a novel recyclable organic acid as catalyst, was employed to pretreat sugarcane bagasse to recover the xylooligosaccharides fraction from hemicellulose and boost the subsequent cellulose saccharification. Results: The FA-assisted hydrolysis of sugarcane bagasse using 3% furoic acid at 170 oC for 15 min resulted in the highest xylooligosaccharides yield of 45.6%; subsequently, 83.1 g/L of glucose was harvested by a fed-batch operation with a solid loading of 15%. Overall, a total of 120 g of xylooligosaccharides and 335 g glucose could be collected from 1000 g sugarcane bagasse starting from the furoic acid pretreatment. Furthermore, furoic acid can be easily recovered by cooling crystallization.Conclusion: This work put forward a novel furoic acid pretreatment method to convert sugarcane bagasse into xylooligosaccharides and glucose, which provides a strategy that the sugar and nutraceutical industries can be used to reduce the production cost. The developed process showed that the yields of xylooligosaccharides and byproducts were controllable by shortening the reaction time; meanwhile, the recyclability of furoic acid also can potentially reduce the pretreatment cost and potentially replace the traditional mineral acids pretreatment.

scholarly journals Effect of Subsequent Dilute Acid and Enzymatic Hydrolysis on Reducing Sugar Production from Sugarcane Bagasse and Spent Citronella Biomass

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Robinson Timung ◽  
Narendra Naik Deshavath ◽  
Vaibhav V. Goud ◽  
Venkata V. Dasu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Crystallinity Index ◽  
Sem Analysis ◽  
Reducing Sugar ◽  
Dilute Acid Pretreatment ◽  
Crystalline Cellulose ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Hydrolysis Of

This work was aimed at investigating the effect of process parameters on dilute acid pretreatment and enzymatic hydrolysis of spent citronella biomass (after citronella oil extraction) and sugarcane bagasse on total reducing sugar (TRS) yield. In acid pretreatment, the parameters studied were acid concentration, temperature, and time. At the optimized condition (0.1 M H2SO4, 120°C, and 120 min), maximum TRS obtained was 452.27 mg·g−1and 487.50 mg·g−1for bagasse and citronella, respectively. Enzymatic hydrolysis of the pretreated biomass usingTrichoderma reesei26291 showed maximum TRS yield of 226.99 mg·g−1for citronella and 282.85 mg·g−1for bagasse at 10 FPU, 50°C, and 48 hr. The maximum crystallinity index (CI) of bagasse and citronella after acid pretreatment obtained from X-ray diffraction analysis was 64.41% and 56.18%, respectively. Decreased CI after enzymatic hydrolysis process to 37.28% and 34.16% for bagasse and citronella, respectively, revealed effective conversion of crystalline cellulose to glucose. SEM analysis of the untreated and treated biomass revealed significant hydrolysis of holocellulose and disruption of lignin.

scholarly journals Optimization of microwave‐assisted alkali pretreatment of cassava rhizome for enhanced enzymatic hydrolysis glucose yield

2019 ◽  
Vol 8 (4) ◽  
Author(s):  
Sakaya Sombatpraiwan ◽  
Tiraporn Junyusen ◽  
Tawarat Treeamnak ◽  
Payungsak Junyusen
Keyword(s):  
Enzymatic Hydrolysis ◽  
Alkali Pretreatment ◽  
Glucose Yield ◽  
Microwave Assisted

scholarly journals Optimization of Sodium Hydroxide Pretreatment and Enzymatic Hydrolysis of Wheat Straw Powder by Cellulases and Xylanases From Aspergillus Niger HQ-1 Using Response Surface Methodology

2021 ◽  
Author(s):  
Hui Zhang ◽  
Junhui Wu
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Aspergillus Niger ◽  
Sodium Hydroxide ◽  
Wheat Straw ◽  
Response Surface ◽  
Sodium Hydroxide Solution ◽  
Solid Loading ◽  
Enzyme Loading ◽  
Hydrolysis Of

Abstract To maximize fermentable sugars production, response surface methodology (RSM) was adopted to optimize pretreatment and enzymatic hydrolysis of wheat straw powder (WSP) using the crude cellulases preparation containing xylanases from Aspergillus niger HQ-1. Factors of pretreatment including sodium hydroxide concentration, pretreatment time and temperature were found to have significant effects on sugars production. Results indicated that WSP with particle size 0.3 mm should be pretreated using 1.8% (w/v) sodium hydroxide solution with 25.0% (w/v) of solid loading at 94.0°C for 46.0 min and the optimized pretreatment conditions could result in 90.9% of cellulose recovery, 54.6% of hemicellulose recovery and 72.7% of lignin removal, respectively. Furthermore, variables of enzymatic hydrolysis including enzyme loading, biomass loading and reaction time were proved to have significant effects on sugars yields. After hydrolysis at 50°C for 44.8 h with 7.1% (w/v) of biomass loading, 8.1 FPU/g of enzyme loading and 0.2% (w/v) of Tween-80, maximum yields of reducing sugar (632.92 mg/g) and xylose (149.83 mg/g) could be obtained, respectively. In addition, holocellulose and hemicellulose conversion were 81.6% and 80.0%, respectively. To the best of our knowledge, this is the first report about systematic optimization of sodium hydroxide pretreatment and enzymatic hydrolysis of WSP using RSM.

scholarly journals Alkaline post-incubation improves the saccharification of poplar after hydrogen peroxide–acetic acid pretreatment

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Peiyao Wen ◽  
Ying Zhang ◽  
Junjun Zhu ◽  
Yong Xu ◽  
Junhua Zhang
Keyword(s):  
Hydrogen Peroxide ◽  
Acetic Acid ◽  
Enzymatic Hydrolysis ◽  
High Concentration ◽  
Acid Pretreatment ◽  
Glucose Yield ◽  
Selective Delignification ◽  
Acetyl Group ◽  
The Cost ◽  
Total Lignin

Abstract Background Hydrogen peroxide–acetic acid (HPAA) is widely used in pretreatment of lignocellulose because it has a good capability in selective delignification. However, high concentration (more than 60%) of HPAA increases the cost of pretreatment and the risk of explosion. In this work, alkaline post-incubation was employed to decrease the HPAA loading and improve the saccharification of poplar. Results Pretreatment with 100% HPAA removed 91.0% lignin and retained 89.9% glucan in poplar. After poplar was pretreated by 100% HPAA at 60 °C for 2 h, the glucan conversion in enzymatic hydrolysis by cellulase increased to 90.1%. Alkaline incubation reduced the total lignin, surface lignin, and acetyl group of HPAA-pretreated poplar. More than 92% acetyl groups of HPAA-pretreated poplar were removed by alkaline incubation with 1.0% NaOH at 50 °C for 1 h. After incubation of 60% HPAA-pretreated poplar with 1.0% NaOH, the glucan conversion enhanced to 95.0%. About 40% HPAA loading in pretreatment was reduced by alkaline incubation without the decrease of glucose yield. Conclusions Alkaline post-incubation had strong ability on the deacetylation and delignification of HPAA-pretreated poplar, exhibiting a strong promotion on the enzymatic hydrolysis yield. This report represented alkaline incubation reduced the HPAA loading, improved pretreatment safety, exhibiting excellent potential application in saccharification of poplar.

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