scholarly journals The Potential Molecular Mechanism of Autologous Adipose Tissue Grafting in Treating Keloid Patients: a Case-control Study

2021 ◽  
Author(s):  
Jiong Zhou ◽  
Ji-Yang Shen ◽  
Li-En Tao ◽  
Huan Chen
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Survival Rate ◽  
Molecular Mechanisms ◽  
Healing Rate ◽  
Adipose Derived Stem Cells ◽  
Promising Alternative ◽  
Keloid Fibroblast ◽  
Keloid Fibroblasts ◽  
Wound Healing Rate

Abstract Background: Keloids represent the dysregulation of cutaneous wound healing caused by aberrant fibroblast activities. Adipose-derived stem cells have been recognized as a promising treatment for keloids. However, their molecular mechanisms have not been fully elucidated. Methods: Skin biopsies were obtained from 10 keloid patients and 9 healthy volunteers. Fibroblasts isolated from all samples were divided into 2 groups, one co-cultured with adipose-derived stem cells, the other growing independently. Between each group, we compared the wound healing rate, fibroblasts’ survival rate, apoptosis rates, mRNA expressions and protein level of Col-1, Col-3, CTGF, P-4-HB. Results: In our research, no significant differences between normal fibroblasts and keloid fibroblasts in terms of wound-healing rate, survival rate, or apoptosis rate were found at the baseline. Adipose-derived stem cells strongly suppressed keloid fibroblasts’ proliferative and invasive behavior, but negatively regulated keloid fibroblast apoptosis. The further measurement of key components in keloid formation showed that adipose-derived stem cells upregulated Col-3 and CTGF levels in normal fibroblasts but downregulated protein expression of CTGF and P-4-HB in keloid fibroblasts. Conclusions: Adipose-derived stem cells had the potential to serve as a promising alternative for keloid treatment.

Role of topical insulin therapy as an adjunct in the management of pressure ulcer

2020 ◽  
Vol 2 (3) ◽  
pp. 01-03
Author(s):  
Ravi Chittoria
Keyword(s):  
Wound Healing ◽  
Insulin Therapy ◽  
Pressure Ulcer ◽  
Healing Rate ◽  
Pressure Sore ◽  
Pressure Sores ◽  
Established Method ◽  
Wound Bed Preparation ◽  
Wound Healing Rate

Pressure ulcer or pressure sore is one of the complications seen in bedridden patients. Management of these ulcers is often challenging. But there is no well-established method that accelerates the wound healing rate. Various adjunctive methods are used for wound bed preparation before definitive reconstruction plan is made. Here we describe our experience in the role of insulin therapy as an adjunct in the management of pressure sores.

Prospective application of exosomes derived from adipose‐derived stem cells in skin wound healing: A review

2019 ◽  
Vol 19 (3) ◽  
pp. 574-581 ◽  
Author(s):  
He Qiu ◽  
Shuo Liu ◽  
Kelun Wu ◽  
Rui Zhao ◽  
Lideng Cao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Skin Wound ◽  
Adipose Derived Stem Cells ◽  
Skin Wound Healing ◽  
Prospective Application

scholarly journals COMPARISON OF WOUND HEALING RATE AND TIME TO COMPLETE WOUND HEALING ACCORDING TO WOUND LOCATION IN CRITICAL LIMB ISCHEMIA

2013 ◽  
Vol 61 (10) ◽  
pp. E2105
Author(s):  
Norihiro Kobayashi ◽  
Muramatsu Toshiya ◽  
Tsukahara Reiko ◽  
Ito Yoshiaki ◽  
Hirano Keisuke
Keyword(s):  
Wound Healing ◽  
Critical Limb Ischemia ◽  
Healing Rate ◽  
Limb Ischemia ◽  
Complete Wound Healing ◽  
Wound Healing Rate

Extracellular vesicles derived from adipose-derived stem cells accelerate diabetic wound healing by suppressing the expression of matrix metalloproteinase-9

2021 ◽  
Vol 22 ◽  
Author(s):  
Jiang-wen Wang ◽  
Yuan-zheng Zhu ◽  
Xuan Hu ◽  
Jia-ying Nie ◽  
Zhao-hui Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mouse Model ◽  
Adipose Derived Stem Cells ◽  
Collagen Deposition ◽  
Diabetic Wound ◽  
Diabetic Wound Healing ◽  
Diabetic Wounds ◽  
Metalloproteinase 9

Background: The healing of diabetic wounds is poor due to a collagen deposition disorder. Matrix metalloproteinase-9 (MMP-9) is closely related to collagen deposition in the process of tissue repair. Many studies have demonstrated that extracellular vesicles derived from adipose-derived stem cells (ADSC-EVs) promote diabetic wound healing by enhancing collagen deposition. Objective: In this study, we explored if ADSC-EVs could downregulate the expression of MMP-9 in diabetic wounds and promote wound healing by improving collagen deposition. The potential effects of ADSC-EVs on MMP-9 and diabetic wound healing were tested both in vitro and in vivo. Methods: We first evaluated the effect of ADSC-EVs on the proliferation and MMP-9 secretion of HaCaT cells treated with advanced glycation end product-bovine serum albumin (AGE-BSA), using CCK-8 western blot and MMP-9 enzyme-linked immunosorbent assay(ELISA). Next, the effect of ADSC-EVs on the healing, re-epithelialisation, collagen deposition, and MMP-9 concentration in diabetic wound fluids was evaluated in an immunodeficient mouse model via MMP-9 ELISA and haematoxylin and eosin, Masson’s trichrome, and immunofluorescence staining for MMP-9. Results: In vitro, ADSC-EVs promoted the proliferation and MMP-9 secretion of HaCaT cells.In vivo, ADSC-EVs accelerated diabetic wound healing by improving re-epithelialisation and collagen deposition and by inhibiting the expression of MMP-9. Conclusion: ADSC-EVs possessed the healing of diabetic wounds in a mouse model by inhibiting downregulating MMP-9 and improving collagen deposition.Thus ,ADSC-EVs are a promising candidate for the treatment of diabetic wounds .

Potential of adipose-derived stem cells harvested from diabetic mice on wound healing

2009 ◽  
Vol 209 (3) ◽  
pp. S89
Author(s):  
Pouya Dastouri ◽  
Rei Ogawa ◽  
Douglas Helm ◽  
Paolo Erba ◽  
Dennis P. Orgill
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Adipose Derived Stem Cells ◽  
Diabetic Mice

scholarly journals The effect of inhibiting exosomes derived from adipose-derived stem cells via the TGF-β1/Smad pathway on the fibrosis of keloid fibroblasts

Gland Surgery ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 1046-1056
Author(s):  
Zhi-Yuan Wu ◽  
Hui-Jun Zhang ◽  
Zhi-Hong Zhou ◽  
Zhan-Peng Li ◽  
Si-Mu Liao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Smad Pathway ◽  
Tgf Β1 ◽  
Keloid Fibroblasts

scholarly journals Treatment of Cutaneous Ulcers with Multilayered Mixed Sheets of Autologous Fibroblasts and Peripheral Blood Mononuclear Cells

2018 ◽  
Vol 47 (1) ◽  
pp. 201-211 ◽  
Author(s):  
Takahiro Mizoguchi ◽  
Koji Ueno ◽  
Yuriko Takeuchi ◽  
Makoto Samura ◽  
Ryo Suzuki ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Healing Rate ◽  
Mononuclear Cells ◽  
Mixed Cell ◽  
Peripheral Blood Mononuclear ◽  
Fibroblast Migration ◽  
Autologous Fibroblasts ◽  
Blood Mononuclear Cells ◽  
Wound Healing Rate

Background/Aims: We have developed a mixed-cell sheet consisting of autologous fibroblasts and peripheral blood mononuclear cells with a high potency for angiogenesis and wound healing against refractory cutaneous ulcers in mouse and rabbit models. To increase the effectiveness of the mixed sheet, we developed a multilayered mixed sheet. Methods: We assessed the therapeutic effects of multilayered sheets on cutaneous ulcers in mice. Growth factors and chemokines were assessed by enzyme-linked immunosorbent assay. Angiogenesis and fibroblast migration were measured by using tube formation and migration assays. Wound healing rate of cutaneous ulcers was evaluated in mice with diabetes mellitus. Results: The concentration of secreted vascular endothelial growth factor, hepatocyte growth factor, transforming growth factor, C-X-C motif chemokine ligand (CXCL)-1, and CXCL-2 in multilayered sheets was much higher than that in single-layered mixed-cell sheets (single-layered sheets) and multilayered sheets of fibroblasts alone (fibroblast sheets). The supernatant in multilayered sheets enhanced angiogenic potency and fibroblast migration compared with single-layered and fibroblast sheets in an in vitro experiment. The wound healing rate in the multilayered sheet-treated group was higher compared with the no-treatment group (control) at the early stage of healing. Moreover, both vessel lumen area and microvessel density in tissues treated with multilayered sheets were significantly increased compared with tissues in the control group. Conclusion: Multilayered sheets promoted wound healing and microvascular angiogenesis in the skin by supplying growth factors and cytokines. Accordingly, our data suggest that multilayered sheets may be a promising therapeutic material for refractory cutaneous ulcers.

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