scholarly journals Simple Strategy for Rapid and Sensitive Detection of 2019 novel coronavirus Based on Antibody

2020 ◽  
Author(s):  
Xinjun Hu ◽  
Huiyang Deng ◽  
Yibing Shang ◽  
Mengnan Fan ◽  
Feng Yue
Keyword(s):  
Colloidal Gold ◽  
Detection Rate ◽  
Detection Sensitivity ◽  
Rt Pcr ◽  
Immunochromatographic Strip ◽  
Simple Strategy ◽  
Immunochromatographic Strip Test ◽  
Pcr Assays ◽  
Novel Coronavirus ◽  
Strip Test

Abstract Objectives: Since December 2019, acute respiratory disease due to 2019 novel coronavirus emerged in Wuhan city and rapidly spread throughout China. Real-time RT-PCR is widely deployed in diagnostic virology. However, the positive detection rates of RT-PCR are only 30% to 50%. Therefore, we propose a simple strategy for rapidly and sensitively detecting the IgM/IgG antibody against 2019-nCoV using a colloidal gold-based immunochromatographic strip test.Methods A total of 41 clinically 2019-nCoV suspected cases (23 males and 18 females) were enrolled. The sensitivity of colloidal gold-based immunochromatographic strip test and of RT-PCR were compared and evaluated. McNemar’s test was used to compare the detection rate of both assays (P<0.05).Results: The Antibody was detected in 63.4% (26/41) of blood specimens using the assay. In contrast, the 2019-nCoV was detected in 46.3% (19/41) of nasal and pharyngeal swab specimens using the RT-PCR assays. The detection rate obtained by this assay was markedly higher than that obtained by the RT-PCR assays (P=0.039)Conclusion: This detection assay exhibits a higher detection sensitivity than RT-PCR. More important, the assay shows the benefits of easy operation and setup. We believe that the sensitive and time-saving approach may be used as an auxiliary diagnostic tool for 2019-nCoV detection and virus screening and confirmation.

scholarly journals Development of a Rapid Fluorescent Immunochromatographic Test to Detect Respiratory Syncytial Virus

2018 ◽  
Vol 19 (10) ◽  
pp. 3013 ◽  
Author(s):  
Trinh Thuy Tien ◽  
Hyun Park ◽  
Hien Tuong ◽  
Seung-Taek Yu ◽  
Du-Young Choi ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Rt Pcr ◽  
Immunochromatographic Strip ◽  
Copy Numbers ◽  
Rsv Infection ◽  
Immunochromatographic Strip Test ◽  
Polymerase Chain ◽  
Syncytial Virus ◽  
Strip Test ◽  
Europium Nanoparticles

Human respiratory syncytial virus (RSV) is one of the most common viruses infecting the respiratory tracts of infants. The rapid and sensitive detection of RSV is important to minimize the incidence of infection. In this study, novel monoclonal antibodies (mAbs; B11A5 and E8A11) against RSV nucleoprotein (NP) were developed and applied to develop a rapid fluorescent immunochromatographic strip test (FICT), employing europium nanoparticles as the fluorescent material. For the FICT, the limits of detection of the antigen and virus were 1.25 µg/mL and 4.23 × 106 TCID50/mL, respectively, corresponding to 4.75 × 106 ± 5.8 ×105 (mean ± SD) RNA copy numbers per reaction mixture for RSV NP. A clinical study revealed a sensitivity of 90% (18/20) and specificity of 98.18% (108/110) for RSV detection when comparing the performance to that of reverse transcription polymerase chain reaction (RT-PCR), representing a 15% improvement in sensitivity over the SD Bioline rapid kit. This newly developed FICT could be a useful tool for the rapid diagnosis of RSV infection.

Stability of control materials for α-thalassemia immunochromatographic strip test

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Paweena Yanpanya ◽  
Chedtapak Ruengdit ◽  
Panida Pongpunyayuen ◽  
Sakorn Pornprasert
Keyword(s):  
Immunochromatographic Strip ◽  
Immunochromatographic Strip Test ◽  
Strip Test

scholarly journals Immunochromatographic Strip Test for Rapid Detection of Nevirapine in Plasma Samples from Human Immunodeficiency Virus-Infected Patients

2007 ◽  
Vol 51 (9) ◽  
pp. 3361-3363 ◽  
Author(s):  
Tim R. Cressey ◽  
Sawitree Nangola ◽  
Yardpiroon Tawon ◽  
Mookda Pattarawarapan ◽  
Marc Lallemant ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Limit Of Detection ◽  
Drug Level ◽  
Plasma Samples ◽  
Immunochromatographic Strip ◽  
Resource Limited ◽  
Qualitative Detection ◽  
Immunodeficiency Virus ◽  
Immunochromatographic Strip Test ◽  
Strip Test

ABSTRACT We report a novel one-step immunochromatographic strip test for the rapid, qualitative detection of nevirapine in plasma samples from human immunodeficiency virus-infected patients. The sensitivity was 100% (95% confidence interval [95% CI], 97.8 to 100%), and the specificity was 99.5% (95% CI, 97.2 to 99.9%). The limit of detection was 25 ng/ml. Immunochromatographic strip tests are simple, rapid, and cheap assays that could greatly facilitate drug level monitoring in resource-limited settings.

Development of a rapid, immunochromatographic strip test for serum asialo α1-acid glycoprotein in patients with hepatic disease

2006 ◽  
Vol 308 (1-2) ◽  
pp. 116-123 ◽  
Author(s):  
Eun Young Lee ◽  
Ji Hyun Kang ◽  
Kyoung A. Kim ◽  
Tai Wha Chung ◽  
Hee Jung Kim ◽  
...  
Keyword(s):  
Hepatic Disease ◽  
Immunochromatographic Strip ◽  
Acid Glycoprotein ◽  
Immunochromatographic Strip Test ◽  
Strip Test

Development of an Immunochromatographic Strip Test for Rapid Detection of Melamine in Raw Milk, Milk Products and Animal Feed

2011 ◽  
Vol 59 (11) ◽  
pp. 6064-6070 ◽  
Author(s):  
Xiangmei Li ◽  
Pengjie Luo ◽  
Shusheng Tang ◽  
Ross C. Beier ◽  
Xiaoping Wu ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Animal Feed ◽  
Raw Milk ◽  
Immunochromatographic Strip ◽  
Milk Products ◽  
Immunochromatographic Strip Test ◽  
Strip Test
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