scholarly journals Multimodal Imaging Probe for Melanoma Evaluating of PTK7 Expression by Sgc8-c Aptamer Recognition

2021 ◽  
Author(s):  
Estefanía Sicco ◽  
Amy Mónaco ◽  
Marcelo Fernandez ◽  
María Moreno ◽  
Victoria Calzada ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Dna Aptamer ◽  
Specific Expression ◽  
Imaging Probe ◽  
Molecular Imaging Probes ◽  
Imaging Probes ◽  
Diagnosis Of Cancer ◽  
Animal Tumor

Abstract Melanoma is one of the most aggressive and deadly skin cancers, and although histopathological criteria are used for its prognosis, biomarkers are necessary to identify the different evolution stages. The applications of molecular imaging include the in vivo diagnosis of cancer with probes that recognize the tumor-biomarkers specific expression allowing external images acquisitions and evaluations of the biological process in quali-quantitative ways. Aptamers are oligonucleotides that recognize targets with high affinity and specificity presenting advantages that make them interesting molecular imaging probes. Sgc8-c (DNA-aptamer) selectively recognizes PTK7-receptor overexpressed in various types of tumors. Herein, Sgc8-c was evaluated, in two melanoma models, non-metastatic and metastatic, as molecular imaging probe for in vivo diagnostic. Firstly, two probes, radio- and fluorescent-probe, were in vitro evaluated verifying the high specific PTK7 recognition and its internalization in tumor cells by the endosomal route. Secondly, in vivo proof of concept was performed in animal tumor models. Likewise, they have rapid clearance from blood exhibiting excellent target (tumor)/non-target organ ratios. Furthermore, optimal biodistribution was observed 24 hours after probes-injections accumulating almost exclusively in the tumor tissue. Sgc8-c is a potential tool for their specific use in the early detection of melanoma.

scholarly journals Metastatic and non-metastatic melanoma imaging using Sgc8-c aptamer PTK7-recognizer

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Estefanía Sicco ◽  
Amy Mónaco ◽  
Marcelo Fernandez ◽  
María Moreno ◽  
Victoria Calzada ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Metastatic Melanoma ◽  
Dna Aptamer ◽  
Specific Expression ◽  
Molecular Imaging Probes ◽  
Imaging Probes ◽  
Melanoma Model ◽  
In Vivo Diagnosis

AbstractMelanoma is one of the most aggressive and deadly skin cancers, and although histopathological criteria are used for its prognosis, biomarkers are necessary to identify the different evolution stages. The applications of molecular imaging include the in vivo diagnosis of cancer with probes that recognize the tumor-biomarkers specific expression allowing external image acquisitions and evaluation of the biological process in quali-quantitative ways. Aptamers are oligonucleotides that recognize targets with high affinity and specificity presenting advantages that make them interesting molecular imaging probes. Sgc8-c (DNA-aptamer) selectively recognizes PTK7-receptor overexpressed in various types of tumors. Herein, Sgc8-c was evaluated, for the first time, in a metastatic melanoma model as molecular imaging probe for in vivo diagnostic, as well as in a non-metastatic melanoma model. Firstly, two probes, radio- and fluorescent-probe, were in vitro evaluated verifying the high specific PTK7 recognition and its internalization in tumor cells by the endosomal route. Secondly, in vivo proof of concept was performed in animal tumor models. In addition, they have rapid clearance from blood exhibiting excellent target (tumor)/non-target organ ratios. Furthermore, optimal biodistribution was observed 24 h after probes injections accumulating almost exclusively in the tumor tissue. Sgc8-c is a potential tool for their specific use in the early detection of melanoma.

scholarly journals Nanobodies as Versatile Tool for Multiscale Imaging Modalities

Biomolecules ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1695
Author(s):  
Marco Erreni ◽  
Tilo Schorn ◽  
Francesca D’Autilia ◽  
Andrea Doni
Keyword(s):  
Molecular Imaging ◽  
Super Resolution ◽  
Imaging Modalities ◽  
Molecular Imaging Probes ◽  
Imaging Probes ◽  
Versatile Tool ◽  
And Function ◽  
Preclinical And Clinical Studies

Molecular imaging is constantly growing in different areas of preclinical biomedical research. Several imaging methods have been developed and are continuously updated for both in vivo and in vitro applications, in order to increase the information about the structure, localization and function of molecules involved in physiology and disease. Along with these progresses, there is a continuous need for improving labeling strategies. In the last decades, the single domain antigen-binding fragments nanobodies (Nbs) emerged as important molecular imaging probes. Indeed, their small size (~15 kDa), high stability, affinity and modularity represent desirable features for imaging applications, providing higher tissue penetration, rapid targeting, increased spatial resolution and fast clearance. Accordingly, several Nb-based probes have been generated and applied to a variety of imaging modalities, ranging from in vivo and in vitro preclinical imaging to super-resolution microscopy. In this review, we will provide an overview of the state-of-the-art regarding the use of Nbs in several imaging modalities, underlining their extreme versatility and their enormous potential in targeting molecules and cells of interest in both preclinical and clinical studies.

scholarly journals Techniques for Molecular Imaging Probe Design

2011 ◽  
Vol 10 (6) ◽  
pp. 7290.2011.00003 ◽  
Author(s):  
Fred Reynolds ◽  
Kimberly A. Kelly
Keyword(s):  
Molecular Imaging ◽  
Target Selection ◽  
Relevant Information ◽  
Probe Design ◽  
Imaging Probe ◽  
Molecular Imaging Probes ◽  
Genomics And Proteomics ◽  
Imaging Probes ◽  
Molecular Imaging Probe ◽  
Underlying Mechanisms

Molecular imaging allows clinicians to visualize disease-specific molecules, thereby providing relevant information in the diagnosis and treatment of patients. With advances in genomics and proteomics and underlying mechanisms of disease pathology, the number of targets identified has significantly outpaced the number of developed molecular imaging probes. There has been a concerted effort to bridge this gap with multidisciplinary efforts in chemistry, proteomics, physics, material science, and biology—all essential to progress in molecular imaging probe development. In this review, we discuss target selection, screening techniques, and probe optimization with the aim of developing clinically relevant molecularly targeted imaging agents.

Recent advances in molecular imaging probes for β-amyloid plaques

MedChemComm ◽  
2015 ◽  
Vol 6 (3) ◽  
pp. 391-402 ◽  
Author(s):  
Masahiro Ono ◽  
Hideo Saji
Keyword(s):  
Molecular Imaging ◽  
Optical Imaging ◽  
Amyloid Plaques ◽  
Recent Advances ◽  
Molecular Imaging Probes ◽  
Imaging Probes ◽  
Β Amyloid ◽  
The Brain

We review recent advances in our development of molecular imaging probes for PET, SPECT, and optical imaging for in vivo detection of β-amyloid plaques in the brain.

scholarly journals Radiolabeled Molecular Imaging Probes for the In Vivo Evaluation of Cellulose Nanocrystals for Biomedical Applications

2018 ◽  
Vol 20 (2) ◽  
pp. 674-683 ◽  
Author(s):  
Surachet Imlimthan ◽  
Sofia Otaru ◽  
Outi Keinänen ◽  
Alexandra Correia ◽  
Kalle Lintinen ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Cellulose Nanocrystals ◽  
Biomedical Applications ◽  
In Vivo Evaluation ◽  
Molecular Imaging Probes ◽  
Imaging Probes

scholarly journals Mitochondrial-Targeted Molecular Imaging in Cardiac Disease

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Jinhui Li ◽  
Jing Lu ◽  
You Zhou
Keyword(s):  
Molecular Imaging ◽  
Cardiac Disease ◽  
Single Photon ◽  
Photon Emission ◽  
Emission Computed Tomography ◽  
Positron Emission ◽  
Molecular Imaging Probes ◽  
Imaging Probes ◽  
Targeted Molecular Imaging

The present study aimed to discuss the role of mitochondrion in cardiac function and disease. The mitochondrion plays a fundamental role in cellular processes ranging from metabolism to apoptosis. The mitochondrial-targeted molecular imaging could potentially illustrate changes in global and regional cardiac dysfunction. The collective changes that occur in mitochondrial-targeted molecular imaging probes have been widely explored and developed. As probes currently used in the preclinical setting still have a lot of shortcomings, the development of myocardial metabolic activity, viability, perfusion, and blood flow molecular imaging probes holds great potential for accurately evaluating the myocardial viability and functional reserve. The advantages of molecular imaging provide a perspective on investigating the mitochondrial function of the myocardium in vivo noninvasively and quantitatively. The molecular imaging tracers of single-photon emission computed tomography and positron emission tomography could give more detailed information on myocardial metabolism and restoration. In this study, series mitochondrial-targeted99mTc-,123I-, and18F-labeled tracers displayed broad applications because they could provide a direct link between mitochondrial dysfunction and cardiac disease.

CLIC1 antibody conjugated nanoscale contrast agent as a sensitive and targeted molecular imaging probe for gallbladder cancer diagnosis

RSC Advances ◽  
2016 ◽  
Vol 6 (29) ◽  
pp. 24104-24110 ◽  
Author(s):  
Wei Lu ◽  
Ning Wang ◽  
YanYan Chu ◽  
Linzhu Zhou ◽  
Maolan Li ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Contrast Agent ◽  
Contrast Agents ◽  
Gallbladder Cancer ◽  
Cancer Diagnosis ◽  
Imaging Probe ◽  
Molecular Imaging Probes ◽  
Imaging Probes ◽  
Molecular Imaging Probe ◽  
Targeted Molecular Imaging

CLIC1 antibody-conjugated nano-scale contrast agents exhibit a fast and sensitive detection of gallbladder tumors and may be used in the future as powerful targeted molecular imaging probes for gallbladder cancer diagnosis.

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