Engineering Elizabethkingia meningoseptica sp. F2 for Vitamin K2 production guided by genome analysis.

2020 ◽  
Author(s):  
Qiang Yang ◽  
zhiming zheng ◽  
Hui Liu ◽  
Peng Wang ◽  
Li Wang ◽  
...  
Keyword(s):  
Metabolic Pathway ◽  
Genome Analysis ◽  
Genomic Sequence ◽  
Efflux Pump ◽  
Synteny Block ◽  
Vitamin K2 ◽  
Evolutionary Status ◽  
Multidrug Efflux Pump ◽  
Elizabethkingia Meningoseptica ◽  
A Genome

Abstract Background The species in family Elizabethkingia meningoseptica are interesting strain for investigating Vitamin K2 metabolic analysis. However, their genomic sequence, metabolic pathway, potential abilities, and evolutionary status are still unknown. Results This study therefore aimed to perform a genome sequencing of Elizabethkingia meningoseptica sp. F2 and further accomplished comparative analysis with other Vitamin K2 strains reveals overall identifying its unique/shared metabolic genes across genomes. The 3,874,794–base pair sequence of Elizabethkingia meningoseptica sp. F2 is presented. Of 3,539 genes annotation was applied. Results of synteny block demonstrated Elizabethkingia meningoseptica sp. F2 shares high levels of synteny with Elizabethkingia meningoseptica ATCC 13253 and Elizabethkingia meningoseptica NBRC 12535. Identification of Vitamin K2 metabolic pathway in Elizabethkingia meningoseptica sp. F2 were also accomplished. In addition, Elizabethkingia meningoseptica sp. F2 was resistant to gentamicin, streptomycin, ampicillin and caramycin, consistent with the presence of multiple genes encoding diverse multidrug efflux pump protein in the genome. Furthermore, By co-overexpression experiments of MenA and MenG, we showed that Vitamin K2 content was enhanced by 37% compared with control strain. Conclusions The genome analysis of Elizabethkingia meningoseptica sp. F2 in conjunction with the comparative metabolic pathways analysis among the E.coli, Bacillus subtilis and Streptomyces provided a useful information on the Vitamin K2 biosynthetic pathway and other related pathways at systems level.

scholarly journals Molecular Insights into an Antibiotic Enhancer Action of New Morpholine-Containing 5-Arylideneimidazolones in the Fight against MDR Bacteria

2021 ◽  
Vol 22 (4) ◽  
pp. 2062
Author(s):  
Aneta Kaczor ◽  
Karolina Witek ◽  
Sabina Podlewska ◽  
Veronique Sinou ◽  
Joanna Czekajewska ◽  
...  
Keyword(s):  
Molecular Modelling ◽  
Efflux Pump ◽  
Gram Negative Bacteria ◽  
Potential Mechanism ◽  
Multidrug Efflux ◽  
Aromatic Rings ◽  
Allosteric Site ◽  
Multidrug Efflux Pump ◽  
Dual Action

In the search for an effective strategy to overcome antimicrobial resistance, a series of new morpholine-containing 5-arylideneimidazolones differing within either the amine moiety or at position five of imidazolones was explored as potential antibiotic adjuvants against Gram-positive and Gram-negative bacteria. Compounds (7–23) were tested for oxacillin adjuvant properties in the Methicillin-susceptible S. aureus (MSSA) strain ATCC 25923 and Methicillin-resistant S. aureus MRSA 19449. Compounds 14–16 were tested additionally in combination with various antibiotics. Molecular modelling was performed to assess potential mechanism of action. Microdilution and real-time efflux (RTE) assays were carried out in strains of K. aerogenes to determine the potential of compounds 7–23 to block the multidrug efflux pump AcrAB-TolC. Drug-like properties were determined experimentally. Two compounds (10, 15) containing non-condensed aromatic rings, significantly reduced oxacillin MICs in MRSA 19449, while 15 additionally enhanced the effectiveness of ampicillin. Results of molecular modelling confirmed the interaction with the allosteric site of PBP2a as a probable MDR-reversing mechanism. In RTE, the compounds inhibited AcrAB-TolC even to 90% (19). The 4-phenylbenzylidene derivative (15) demonstrated significant MDR-reversal “dual action” for β-lactam antibiotics in MRSA and inhibited AcrAB-TolC in K. aerogenes. 15 displayed also satisfied solubility and safety towards CYP3A4 in vitro.

scholarly journals Correlation between the Antibiotic Resistance Genes and Susceptibility to Antibiotics among the Carbapenem-Resistant Gram-Negative Pathogens

Antibiotics ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 255
Author(s):  
Salma M. Abdelaziz ◽  
Khaled M. Aboshanab ◽  
Ibrahim S. Yahia ◽  
Mahmoud A. Yassien ◽  
Nadia A. Hassouna
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Efflux Pump ◽  
Antibiotic Resistance Genes ◽  
Multiple Drug ◽  
P Value ◽  
Multidrug Efflux ◽  
Multidrug Efflux Pump ◽  
Gram Negative ◽  
Carbapenem Resistant

In this study, the correlation between the antibiotic resistance genes and antibiotic susceptibility among the carbapenem-resistant Gram-negative pathogens (CRGNPs) recovered from patients diagnosed with acute pneumonia in Egypt was found. A total of 194 isolates including Klebsiella pneumoniae (89; 46%), Escherichia coli (47; 24%) and Pseudomonas aeruginosa (58; 30%) were recovered. Of these, 34 (18%) isolates were multiple drug resistant (MDR) and carbapenem resistant. For the K. pneumoniae MDR isolates (n = 22), blaNDM (14; 64%) was the most prevalent carbapenemase, followed by blaOXA-48 (11; 50%) and blaVIM (4; 18%). A significant association (p value < 0.05) was observed between the multidrug efflux pump (AcrA) and resistance to β-lactams and the aminoglycoside acetyl transferase gene (aac-6’-Ib) gene and resistance to ciprofloxacin, azithromycin and β-lactams (except for aztreonam). For P. aeruginosa, a significant association was noticed between the presence of the blaSHV gene and the multidrug efflux pump (MexA) and resistance to fluoroquinolones, amikacin, tobramycin, co-trimoxazole and β-lactams and between the aac-6’-Ib gene and resistance to aminoglycosides. All P. aeruginosa isolates (100%) harbored the MexAB-OprM multidrug efflux pump while 86% of the K. pneumoniae isolates harbored the AcrAB-TolC pump. Our results are of great medical importance for the guidance of healthcare practitioners for effective antibiotic prescription.

scholarly journals Na+-driven multidrug efflux pump VcmA fromVibrio choleraenon-O1, a non-halophilic bacterium

2001 ◽  
Vol 203 (2) ◽  
pp. 235-239 ◽  
Author(s):  
M.Nazmul Huda ◽  
Yuji Morita ◽  
Teruo Kuroda ◽  
Tohru Mizushima ◽  
Tomofusa Tsuchiya
Keyword(s):  
Efflux Pump ◽  
Halophilic Bacterium ◽  
Multidrug Efflux ◽  
Multidrug Efflux Pump

Detecting Substrates Bound to the Secondary Multidrug Efflux Pump EmrE by DNP-Enhanced Solid-State NMR

2013 ◽  
Vol 135 (42) ◽  
pp. 15754-15762 ◽  
Author(s):  
Yean Sin Ong ◽  
Andrea Lakatos ◽  
Johanna Becker-Baldus ◽  
Klaas M. Pos ◽  
Clemens Glaubitz
Keyword(s):  
Solid State ◽  
Solid State Nmr ◽  
Efflux Pump ◽  
Multidrug Efflux ◽  
Multidrug Efflux Pump

scholarly journals Genetic Analysis of Functions Involved in Adhesion of Pseudomonas putida to Seeds

2000 ◽  
Vol 182 (9) ◽  
pp. 2363-2369 ◽  
Author(s):  
Manuel Espinosa-Urgel ◽  
Amparo Salido ◽  
Juan-Luis Ramos
Keyword(s):  
Pseudomonas Putida ◽  
Calcium Binding ◽  
Efflux Pump ◽  
Protein A ◽  
Surface Proteins ◽  
Peptide Transporter ◽  
Multidrug Efflux Pump ◽  
Bacterial Populations ◽  
Abiotic Surfaces ◽  
Initial Seed

ABSTRACT Many agricultural uses of bacteria require the establishment of efficient bacterial populations in the rhizosphere, for which colonization of plant seeds often constitutes a critical first step.Pseudomonas putida KT2440 is a strain that colonizes the rhizosphere of a number of agronomically important plants at high population densities. To identify the functions involved in initial seed colonization by P. putida KT2440, we subjected this strain to transposon mutagenesis and screened for mutants defective in attachment to corn seeds. Eight different mutants were isolated and characterized. While all of them showed reduced attachment to seeds, only two had strong defects in their adhesion to abiotic surfaces (glass and different plastics). Sequences of the loci affected in all eight mutants were obtained. None of the isolated genes had previously been described in P. putida, although four of them showed clear similarities with genes of known functions in other organisms. They corresponded to putative surface and membrane proteins, including a calcium-binding protein, a hemolysin, a peptide transporter, and a potential multidrug efflux pump. One other showed limited similarities with surface proteins, while the remaining three presented no obvious similarities with known genes, indicating that this study has disclosed novel functions.

scholarly journals Gene Cloning and Characterization of VcrM, a Na+-Coupled Multidrug Efflux Pump, fromVibrio choleraeNon-O1

2003 ◽  
Vol 47 (6) ◽  
pp. 419-427 ◽  
Author(s):  
Md. Nazmul Huda ◽  
Jing Chen ◽  
Yuji Morita ◽  
Teruo Kuroda ◽  
Tohru Mizushima ◽  
...  
Keyword(s):  
Gene Cloning ◽  
Efflux Pump ◽  
Multidrug Efflux ◽  
Multidrug Efflux Pump ◽  
Gene Cloning And Characterization

scholarly journals AcrAB Multidrug Efflux Pump Is Associated with Reduced Levels of Susceptibility to Tigecycline (GAR-936) in Proteus mirabilis

2003 ◽  
Vol 47 (2) ◽  
pp. 665-669 ◽  
Author(s):  
Melissa A. Visalli ◽  
Ellen Murphy ◽  
Steven J. Projan ◽  
Patricia A. Bradford
Keyword(s):  
Proteus Mirabilis ◽  
Efflux Pump ◽  
Wild Type ◽  
Multidrug Efflux ◽  
Multidrug Efflux Pump ◽  
Transposon Insertion ◽  
Spectrum Activity ◽  
Insertion Mutants ◽  
Broad Spectrum Activity ◽  
Increased Susceptibility

ABSTRACT Tigecycline has good broad-spectrum activity against many gram-positive and gram-negative pathogens with the notable exception of the Proteeae. A study was performed to identify the mechanism responsible for the reduced susceptibility to tigecycline in Proteus mirabilis. Two independent transposon insertion mutants of P. mirabilis that had 16-fold-increased susceptibility to tigecycline were mapped to the acrB gene homolog of the Escherichia coli AcrRAB efflux system. Wild-type levels of decreased susceptibility to tigecycline were restored to the insertion mutants by complementation with a clone containing a PCR-derived fragment from the parental wild-type acrRAB efflux gene cluster. The AcrAB transport system appears to be associated with the intrinsic reduced susceptibility to tigecycline in P. mirabilis.

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