Degradation of Chitin using Chitinase Produced from Molecular Identified Bacteria

Mapping Intimacies ◽

10.21203/rs.3.rs-269043/v1 ◽

2021 ◽

Author(s):

Magda M Aly ◽

Amna A Seddiq ◽

Afra M Baghdadi

Keyword(s):

Saudi Arabia ◽

Fungal Pathogens ◽

Crop Production ◽

Agar Medium ◽

Western Region ◽

Biotechnological Applications ◽

Carbon And Nitrogen ◽

Chitinolytic Bacteria ◽

Different Sources

Abstract Chitin and chitinolytic are beneficial enzymes for their biotechnological applications. Chitinases contribute to the generation of carbon and nitrogen in the ecosystem and can prohibit many fungal ailments that can threaten crop production worldwide. The goal of this work was the production and characterization of chitinase enzymes from bacteria isolated from the western region, Saudi Arabia for biocontrol fungal pathogens. Colloidal chitin from shrimp shells was prepared and used for isolation of chitinolytic bacteria on Mineral chitin agar medium from different sources. The most active isolates were AMM1which was characterized and identified as Alcaligenes aquatilis using 16SrRNA.. In conclusion, purified chitinase was success to produce from Alcaligenes aquatilis for medical uses and biocontrol process.

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Erratum: Prevalence and characterization of urolithiasis in the Western region of Saudi Arabia

Urology Annals ◽

10.4103/0974-7796.282513 ◽

2020 ◽

Vol 12 (2) ◽

pp. 203

Keyword(s):

Saudi Arabia ◽

Western Region ◽

The Western Region

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Isolation, selection, and characterization of beneficial rhizobacteria from pea, lentil, and chickpea grown in western Canada

Canadian Journal of Microbiology ◽

10.1139/w08-008 ◽

2008 ◽

Vol 54 (4) ◽

pp. 248-258 ◽

Cited By ~ 72

Author(s):

Russell K. Hynes ◽

Grant C.Y. Leung ◽

Danielle L.M. Hirkala ◽

Louise M. Nelson

Keyword(s):

Plant Growth ◽

Plant Growth Promotion ◽

Fungal Pathogens ◽

Crop Production ◽

Growth Promotion ◽

Profile Analysis ◽

Acc Deaminase ◽

Plant Growth Promoting ◽

Growth Promoting

The use of beneficial soil microorganisms as agricultural inputs for improved crop production requires selection of rhizosphere-competent microorganisms with plant growth-promoting attributes. A collection of 563 bacteria originating from the roots of pea, lentil, and chickpea grown in Saskatchewan was screened for several plant growth-promoting traits, for suppression of legume fungal pathogens, and for plant growth promotion. Siderophore production was detected in 427 isolates (76%), amino-cyclopropane-1-carboxylic acid (ACC) deaminase activity in 29 isolates (5%), and indole production in 38 isolates (7%). Twenty-six isolates (5%) suppressed the growth of Pythium sp. strain p88-p3, 40 isolates (7%) suppressed the growth of Fusarium avenaceum , and 53 isolates (9%) suppressed the growth of Rhizoctonia solani CKP7. Seventeen isolates (3%) promoted canola root elongation in a growth pouch assay, and of these, 4 isolates promoted the growth of lentil and one isolate promoted the growth of pea. Fatty acid profile analysis and 16S rRNA sequencing of smaller subsets of the isolates that were positive for the plant growth-promotion traits tested showed that 39%–42% were members of the Pseudomonadaceae and 36%–42% of the Enterobacteriaceae families. Several of these isolates may have potential for development as biofertilizers or biopesticides for western Canadian legume crops.

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