scholarly journals Characterization of LC-MS Based Urine Metabolomics in Healthy Subjects Across the Life Span

2020 ◽  
Author(s):  
Xiaoyan Liu ◽  
Xiaoyi Tian ◽  
Qinghong Shi ◽  
Haidan Sun ◽  
Jing Li ◽  
...  
Keyword(s):  
Biomarker Discovery ◽  
High Resolution Mass Spectrometry ◽  
Estrogen Metabolism ◽  
Healthy Children ◽  
Urine Metabolites ◽  
Clinical Cohort ◽  
Disease Biomarker ◽  
Gender And Age ◽  
Urine Metabolomics

Abstract Background: Previous studies reported that gender and age could influence urine metabolomics, which should be considered in biomarker discovery. As a consequence, for the baseline of urine metabolomics characteristics, it becomes critical to avoid confounding effects in clinical cohort studies. Results: In this study, we provided a comprehensive lifespan characterization of urine metabolomics in a cohort of 348 healthy children and 315 adults aged 1 to 70 years using liquid chromatography coupled with high resolution mass spectrometry. Our results suggested that gender-dependent urine metabolites are much greater in adults than in children. The pantothenate and CoA biosynthesis and alanine metabolism pathways were enriched in early life. Androgen and estrogen metabolism showed high activity during adolescence and youth stages. Pyrimidine metabolism was enriched in the old stage. Conclusion: Based on the above analysis, metabolomic characteristics of each age stage were provided. This work could help us understand the baseline of urine metabolism characteristics and contribute to further studies of clinical disease biomarker discovery.

scholarly journals Characterization of LC-MS based urine metabolomics in healthy subjects across the life span

2020 ◽  
Author(s):  
Xiaoyan Liu ◽  
Xiaoyi Tian ◽  
Qinghong Shi ◽  
Haidan Sun ◽  
Jing Li ◽  
...  
Keyword(s):  
Biomarker Discovery ◽  
High Resolution Mass Spectrometry ◽  
Estrogen Metabolism ◽  
Healthy Children ◽  
Urine Metabolites ◽  
Clinical Cohort ◽  
Disease Biomarker ◽  
Gender And Age ◽  
Urine Metabolomics

AbstractPrevious studies reported that gender and age could influence urine metabolomics, which should be considered in biomarker discovery. As a consequence, for the baseline of urine metabolomics characteristics, it becomes critical to avoid confounding effects in clinical cohort studies. In this study, we provided a comprehensive lifespan characterization of urine metabolomics in a cohort of 348 healthy children and 315 adults aged 1 to 70 years using liquid chromatography coupled with high resolution mass spectrometry. Our results suggested that gender-dependent urine metabolites are much greater in adults than in children. The pantothenate and CoA biosynthesis and alanine metabolism pathways were enriched in early life. Androgen and estrogen metabolism showed high activity during adolescence and youth stages. Pyrimidine metabolism was enriched in the old stage. Based on the above analysis, metabolomic characteristics of each age stage were provided. This work could help us understand the baseline of urine metabolism characteristics and contribute to further studies of clinical disease biomarker discovery.

scholarly journals Characterization of LC-MS Based Urine Metabolomics in Healthy Children and Adults

2021 ◽  
Author(s):  
Xiaoyan Liu ◽  
Xiaoyi Tian ◽  
Qinghong Shi ◽  
Haidan Sun ◽  
Jing Li ◽  
...  
Keyword(s):  
Biomarker Discovery ◽  
Estrogen Metabolism ◽  
Healthy Children ◽  
Age Group ◽  
Urine Metabolites ◽  
Disease Biomarker ◽  
Gender And Age ◽  
Comprehensive Characterization ◽  
Urine Metabolomics

Abstract Previous studies reported that gender, age, diets or lifestyles could influence urine metabolomics, which should be considered in biomarker discovery. As a consequence, for the baseline of urine metabolomics characteristics, it becomes critical to avoid confounding effects in clinical cohort studies. In this study, we provided a comprehensive characterization of urine metabolomics in a cohort of 348 healthy children (Aged 1~18) and 315 adults (Aged 20-78) for evaluation gender and age effects. Our results suggested that urine metabolites showed larger gender differences in children than in adults. For both male/boy and female/girl, each age group showed specific metabolic characterization. Especially, the pantothenate and CoA biosynthesis and alanine metabolism pathways were enriched in early life. Androgen and estrogen metabolism showed high activity during adolescence and youth stages. Pyrimidine metabolism was enriched in the old stage. This work could help us understand the baseline of urine metabolism characteristics and contribute to further studies of clinical disease biomarker discovery.

scholarly journals Grape Seeds Proanthocyanidins: Advanced Technological Preparation and Analytical Characterization

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 418
Author(s):  
Paolo Morazzoni ◽  
Paola Vanzani ◽  
Sandro Santinello ◽  
Antonina Gucciardi ◽  
Lucio Zennaro ◽  
...  
Keyword(s):  
High Resolution Mass Spectrometry ◽  
Industrial Process ◽  
Gel Permeation Chromatography ◽  
Grape Seed ◽  
Direct Infusion ◽  
Grape Seeds ◽  
Flow Filtration ◽  
Process Patent ◽  
Shotgun Approach

A “green” solvent-free industrial process (patent pending) is here described for a grape seed extract (GSE) preparation (Ecovitis™) obtained from selected seeds of Veneto region wineries, in the northeast of Italy, by water and selective tangential flow filtration at different porosity. Since a comprehensive, non-ambiguous characterization of GSE is still a difficult task, we resorted to using an integrated combination of gel permeation chromatography (GPC) and electrospray ionization high resolution mass spectrometry (ESI-HRMS). By calibration of retention time and spectroscopic quantification of catechin as chromophore, we succeeded in quantifying GPC polymers up to traces at n = 30. The MS analysis carried out by the ESI-HRMS method by direct-infusion allows the detection of more than 70 species, at different polymerization and galloylation, up to n = 13. This sensitivity took advantage of the nanoscale shotgun approach, although paying the limit of missed separation of stereoisomers. GPC and MS approaches were remarkably well cross-validated by overlapping results. This simple integrated analytical approach has been used for quality control of the production of Ecovitis™. The emerging feature of Ecovitis™ vs. a popular benchmark in the market, produced by a different technology, is the much lower content of species at low n and the corresponding increase of species at high n.

scholarly journals A combined flow injection/reversed phase chromatography – high resolution mass spectrometry workflow for accurate absolute lipid quantification with 13C- internal standards

The Analyst ◽  
2021 ◽  
Author(s):  
Harald Schoeny ◽  
Evelyn Rampler ◽  
Yasin El Abiead ◽  
Felina Hildebrand ◽  
Olivia Zach ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Flow Injection ◽  
High Resolution Mass Spectrometry ◽  
Reversed Phase ◽  
Internal Standards ◽  
Lipid Quantification ◽  
High Resolution Mass ◽  
Resolution Mass

We propose a fully automated novel workflow for lipidomics based on flow injection- followed by liquid chromatography high resolution mass spectrometry (FI/LC-HRMS). The workflow combined in-depth characterization of the lipidome...

Synthesis and characterization of meso-to-meso directly linked porphyrin-diazaporphyrin triads

2018 ◽  
Vol 22 (09n10) ◽  
pp. 814-820
Author(s):  
Yingying Jia ◽  
Ling Xu ◽  
Bangshao Yin ◽  
Mingbo Zhou ◽  
Jianxin Song
Keyword(s):  
Nickel Complex ◽  
High Resolution Mass Spectrometry ◽  
Dihedral Angles ◽  
X Ray Diffraction ◽  
Extinction Coefficients ◽  
X Ray ◽  
H Nmr ◽  
First Time ◽  
Molar Extinction Coefficients

Beginning with 5,10,15-triarylporphyrin-nickel complex, five meso-to-meso directly linked porphyrin-diazaporphyrin triads were successfully prepared for the first time through a series of reactions including formylation via Vilsmeier–Haack reaction, condensation with pyrrole, bromination with [Formula: see text]-Bromosuccinimide (NBS), oxidation with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ), metal-templated cyclization of dibromodipyrrin-metal complexes with NaN[Formula: see text] and demetalization. All these triads were comprehensively characterized by [Formula: see text]H NMR, high-resolution mass spectrometry and UV-vis absorption. In addition, the structure of compound 6Ni was unambiguously determined by X-ray diffraction analysis, which showed that the two dihedral angles are both 86.65 (4)[Formula: see text] between each mean plane of porphyrin and that of central diazaporphyrin The UV-vis absorption spectra disclosed that the longest wavelengths of Soret bands and Q bands for these triads were observed at 429 and 642 nm, respectively. In contrast to diazaporphyrin-porphyrin dyads, diazaporphyrin dimers and diazaporphyrin monomers reported previously the molar extinction coefficients, particularly for triad 8Ni are much higher.

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